Methods for increasing wheat grain protein content and sds sedimentation value and associated haplotypes and applications

By regulating the rez haplotype gene on wheat chromosome 4B, especially by knocking out or reducing the expression of the gene encoding Rht-B1b, EamA-B, and ZnF-B proteins, and using CRISPR-Cas9 technology and Agrobacterium-mediated transformation, the problems of low wheat grain protein content and SDS sedimentation value were solved, and wheat breeding with high protein and high SDS sedimentation value was achieved.

CN122146752APending Publication Date: 2026-06-05CHINA AGRI UNIV
View PDF 0 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHINA AGRI UNIV
Filing Date
2026-02-02
Publication Date
2026-06-05

AI Technical Summary

Technical Problem

How to improve the quality of wheat grains and/or increase the protein content and/or SDS sedimentation value of wheat grains.

Method used

By regulating the expression of rez haplotype genes on wheat chromosome 4B, including knocking out or reducing the expression of genes encoding Rht-B1b, EamA-B, and ZnF-B proteins, gene editing was performed using CRISPR-Cas9 technology, combined with Agrobacterium-mediated transgenic breeding, to cultivate wheat with high grain protein content and high SDS sedimentation value.

Benefits of technology

It significantly improved the total protein content and SDS sedimentation value of wheat grains, laying a technical foundation for breeding high-quality wheat varieties and providing a method for detecting rez haplotypes and grain quality.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure FT_1
    Figure FT_1
  • Figure FT_2
    Figure FT_2
  • Figure FT_3
    Figure FT_3
Patent Text Reader

Abstract

The application discloses a method for improving wheat kernel protein content and SDS sedimentation value and related haplotypes and application thereof. r-e-z The application first finds a very rare haplotype natural variation in wheat varieties, which has the function of regulating the related traits of wheat kernel protein content and SDS sedimentation value. r-e-z The haplotype corresponds to the deletion of three proteins, Rht-B1b, EamA-B and ZnF-B, corresponding to the genes in the 4B chromosome of wheat. r-e-z The haplotype or the knockout of the Rht-B1b gene in the wheat genome can simultaneously improve the wheat kernel protein content and the SDS sedimentation value. The application can be used for cultivating new wheat varieties with high quality and high yield in agricultural production.
Need to check novelty before this filing date? Find Prior Art