A device for testing the wet damage tolerance of rape

Abstract

The present application relates to a kind of rape wet damage tolerance experimental device, including two planting boxes, two planting boxes are all equipped with photosynthometer, planting groove, light source, temperature sensor, air conditioning system, humidity sensor, humidity regulator and water supply system;The method for carrying out rape wet damage tolerance evaluation is: in two planting grooves, all rape is planted, the temperature, humidity and illumination of two planting boxes are kept same, the soil of the rape in one planting groove is flooded by water at 3-4 leaf stage and is planted to wet damage to test end, another planting groove is planted healthily, on the fifth day and the sixth day of water flooding, the average number of two days of stomatal conductance of wet damage planting rape and healthy planting rape is obtained by the stomatal conductance detection of photosynthometer to two planting boxes.This application aims to provide a kind of rape wet damage tolerance experimental device, which is environment-controllable and can quickly and accurately judge the wet tolerance of rape, solves the problem that the environment is uncontrollable when testing the wet tolerance of rape, the data accuracy is poor and the experimental time is long.

Description

Technical Field

[0001] This invention relates to the field of agricultural breeding technology, and in particular to an experimental device for rapeseed's tolerance to moisture damage. Background Technology

[0002] In areas with abundant rainfall, rapeseed is highly susceptible to waterlogging (i.e., water damage) during its seedling and flowering stages. Water damage leads to oxygen deficiency in the rapeseed roots, closure of leaf stomata, and inhibition of photosynthesis, ultimately resulting in severe yield reduction. Therefore, screening and cultivating rapeseed varieties with strong water resistance is of great significance for ensuring the stability of rapeseed production.

[0003] Currently, the assessment of rapeseed's moisture tolerance mainly employs field flooding tests or indoor pot flooding tests. However, these methods have the following drawbacks: 1) Field tests are affected by uncontrollable factors such as climate and soil heterogeneity, resulting in poor repeatability and making it difficult to accurately compare the true moisture tolerance of different seeds; 2) While indoor pot tests offer controllable conditions, they often lack relatively consistent healthy controls and flooding environments (consistent temperature, humidity, and light for rapeseed seedling treatment, as well as consistent flooding height and management practices); 3) Evaluation indicators largely rely on changes in crop morphology (such as plant mortality, plant height, biomass, root growth, leaf photosynthetic rate, chlorophyll content, alcohol dehydrogenase activity, antioxidant enzyme activity, and cell membrane permeability) or final yield, which is time-consuming. The current evaluation methods for rapeseed moisture tolerance are characterized by several drawbacks: 1) Labor-intensive, long implementation period, and destructive sampling (e.g., measuring root length may require digging up the soil and damaging the crop); 2) Long experimental periods (requiring a complete crop growth cycle for evaluation based on final yield); and 3) Unclear evaluation mechanisms (plant height and biomass are superficial indicators and cannot reflect the intrinsic moisture tolerance mechanism). 4) The evaluation methods mainly utilize principal component analysis and membership function methods to comprehensively evaluate germplasm materials and determine their moisture tolerance level. This method is complex to operate, requires a high level of statistical expertise from the operator, and its application value may have certain limitations. 5) Current moisture tolerance evaluations are mostly qualitative and graded, lacking quantitative evaluation. Most current evaluation methods divide moisture tolerance into high, medium, and low levels, and seeds of the same level may not be comparable in terms of moisture tolerance. Therefore, there is an urgent need for a device that can simulate a stable wet environment, provide synchronous controls, and rapidly, non-destructively, and quantitatively evaluate the moisture tolerance of rapeseed. Summary of the Invention

[0004] The present invention aims to provide an experimental device for testing rapeseed's resistance to moisture damage in a controllable environment and to quickly and accurately determine the rapeseed's resistance to moisture, thus solving the problems of uncontrollable environment, poor data accuracy, and long experimental time in existing tests of rapeseed's resistance to moisture.

[0005] The above technical problems are solved by the following technical solution: A rapeseed moisture tolerance experimental device, characterized in that it includes two planting boxes, each of which is equipped with a photosynthesis unit, a planting trough, a light source, a temperature sensor, an air conditioning system, a humidity sensor, a humidity regulator, and a water supply system; the method for evaluating rapeseed moisture tolerance is as follows: rapeseed is planted in both planting troughs, and light is provided to the rapeseed in the planting troughs through the light source; the air conditioning system adjusts the temperature in the planting box to a set value; the temperature sensor detects the temperature in the planting box; the humidity sensor detects the humidity in the planting box; and the humidity regulator adjusts the temperature in the planting box to a set value. The humidity in the planting boxes was adjusted to the set value, and the water supply system supplied water to the rapeseed in the planting troughs. Temperature, humidity, and light were kept identical in both planting boxes. In one planting trough, the rapeseed was submerged in water to a depth of 1-2 cm during the 3-4 leaf stage for waterlogging treatment until the end of the experiment. The other planting trough was provided with a normal growing environment for healthy cultivation. On the fifth and sixth days after submersion, stomatal conductance was measured in both planting boxes using a photosynthesis meter. The average stomatal conductance (Gs1) for the waterlogged rapeseed and the average stomatal conductance (Gs2) for the healthy rapeseed were obtained over two days. The moisture tolerance coefficient (M) was calculated using the formula M = Gs1 / Gs2. The moisture tolerance of the rapeseed was then graded: M ≥ 70% was highly moisture-tolerant, 55% ≤ M < 70% was moisture-tolerant, 40% ≤ M < 55% was moderate, and M < 40% was sensitive. Evaluating moisture tolerance by measuring stomatal conductance is highly accurate.

[0006] Preferably, the planting trough has two cover plates distributed along its width for sealing the trough. Between the two cover plates are several rapeseed stalk passages distributed along the length of the planting trough when the cover plates are closed, allowing the rapeseed stalks in the planting trough to pass through one-to-one. The diameter of each rapeseed stalk passage is less than 2 cm larger than the diameter of the rapeseed stalk. At the end of the experiment, the rapeseed is removed by closing the two cover plates and then pulling it out of the soil in the planting trough. The rapeseed roots are restricted as they pass through the rapeseed stalk passages, causing the soil attached to the roots to be scraped off, thus minimizing the amount of soil removed during removal and facilitating a second experiment. When planting rapeseed, it should be aligned with the rapeseed stalk passages.

[0007] Preferably, the lower end of the rapeseed stalk through the hole is provided with a downward-facing annular cutting blade. This allows for easier scraping of the soil attached to the roots.

[0008] Preferably, when the two cover plates are closed, the side where the cover plates are connected is higher than the other side. This allows for an acceleration period between the rapeseed being pulled out of the soil and contacting the annular cutting blade, thus enabling the soil to be scraped off more reliably and effortlessly.

[0009] Preferably, the planting trough is equipped with a soil-loosening mechanism to loosen the soil within the trough. This allows for soil tilling during secondary planting experiments.

[0010] Preferably, the soil loosening mechanism includes a transverse cutting strip extending along the width of the planting trough and a vertical cutting strip connected at its lower end to the transverse cutting strip. The transverse cutting strip has transversely extending horizontal cutting edges on both its front and rear sides, and the vertical cutting strip has vertically extending vertical cutting edges on both its front and rear sides. The width of the planting trough is equal from top to bottom. A guide groove is provided on the inner surface of the sidewall of the planting trough along its width. Both ends of the transverse cutting strip pass through the guide grooves on the two sidewalls of the planting trough along its width. The guide groove includes several vertical segments and several horizontally extending segments extending along the front-rear direction and distributed vertically. Three adjacent segments... The horizontal extension section has one end connected to one end of the upper horizontal extension section via a vertical section, and the other end connected to one end of the lower horizontal extension section via a vertical section. A first drive wheel is located at the lower end of the guide groove, and a second drive wheel is located at the upper end. The second drive wheel is connected to a drive motor. One end of the traction belt is connected to the horizontal blade, and the other end passes around the first and second drive wheels before connecting to the horizontal blade. The traction belt passes through the guide groove, and the length of the vertical blade is greater than the distance between two adjacent horizontal extension ends. The drive motor drives the traction belt in conjunction with the guide groove to move the horizontal and vertical cutting blades horizontally and vertically, thereby ensuring that all the soil within the planting box is tilled.

[0011] Preferably, the upper and lower ends of the inner wall of the vertical channel are provided with guide rounded corners, which are metal structures.

[0012] Preferably, the planting trough also includes a planting trough displacement structure. The planting trough, used to simulate a wetland environment for wetland planting, is equipped with a water level control structure. The water level control structure is used to control the water level of the submerged soil in the planting trough at a set height. The bottom wall of the planting trough is composed of two horizontally distributed halves. On the outer side of the two halves, hinged to the planting box body, the lower right surface is provided with two longitudinally distributed door latches. The door latches on the two halves are aligned one-to-one. The two aligned door latches are fitted onto a latch so that the bottom wall of the planting trough is kept in the whole that closes the lower end of the planting trough body. The top wall of the planting box is provided with an openable door panel aligned with the planting trough. The planting box displacement structure is used to move one planting box to be located directly above another planting box. After tilling, a planting trough displacement mechanism is used to position one planting trough above another. Then, the latch of the upper planting trough is pulled out, causing the soil in the upper trough to fall into the lower planting trough. The latch is then reinserted, closing the bottom wall of the upper planting trough and moving both troughs to their initial positions. Next, another planting trough is moved above a single planting trough, and the latch of the upper planting trough is pulled out, causing half of the soil in the upper planting trough to fall into the lower planting trough. The latch is then reinserted, closing the bottom wall of the upper planting trough, thus achieving soil rotation. A second experiment is then conducted on the same rapeseed. This method reduces the influence of soil on the experiment.

[0013] Preferably, the planting box displacement structure includes a crossbeam located above the two planting boxes, a sliding sleeve fitted onto the crossbeam, a hoist connected to the sliding sleeve, and a lifting cable with a hook connected to the hoist. The planting trough has lifting rings on both sides that cooperate with the hooks. In use, the hooks are hooked onto the lifting rings of one planting box, the hoist lifts one planting box higher than the other, and then the sliding sleeve moves on the crossbeam so that one planting box is directly above the other, opening the bottom wall of one planting box and allowing soil to fall into it.

[0014] Preferably, the planting trough is elongated, with only one row of rapeseed planted inside. The upper end of the trough has two cover plates distributed along its width for sealing the trough. Between the two cover plates are several rapeseed stalk passage holes distributed along the length of the trough when the cover plates are closed, allowing the rapeseed stalks to pass through one-to-one. The diameter of each rapeseed stalk passage hole is less than 2 cm larger than the diameter of the rapeseed stalk. The lower end of the planting trough has a support protrusion, and the cover plates have positioning grooves for the latch to pass through. This design ensures that the cover plates and the latch do not interfere with each other.

[0015] The present invention has the following advantages: it designs a parallel dual-box structure of "wet damage treatment + healthy control", and for the first time achieves strict consistency of all environmental factors except moisture factor in a simulated environment, eliminates environmental error, and significantly improves the accuracy and repeatability of the evaluation. For the first time, stomatal conductance was proposed as the core physiological indicator, and a quantitative evaluation standard, the "moisture tolerance coefficient," was constructed. Stomatal conductance is one of the most sensitive physiological indicators of plant response to water stress. Compared with traditional morphological indicators, this method is faster and more sensitive, and can accurately judge the moisture tolerance potential of germplasm in the early stages of stress. The apparatus and method of this invention can be widely applied to the large-scale screening of rapeseed breeding materials, the study of the genetic laws of moisture tolerance, and the discovery of moisture tolerance genes, providing a powerful tool and technical support for accelerating the breeding process of new moisture-tolerant rapeseed varieties. This invention has significant demonstrative innovation. The structure and method of this invention can also be used as a reference for screening the stress resistance of other crop breeding materials. At that time, only the relevant environment and evaluation parameters need to be adjusted. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the present invention; Figure 2 for Figure 1 A magnified view of a portion of point A; Figure 3 for Figure 1 A magnified view of a portion of point B; Figure 4 for Figure 1 A magnified view of part C. Figure 5 This is a schematic diagram of the sidewall of the planting trough; Figure 6 for Figure 5 A magnified view of a portion at point D; Figure 7 for Figure 5 A magnified view of a portion at point E; Figure 8 A schematic diagram of removing rapeseed while closing the cover plate.

[0017] In the diagram: 1. Planting box; 2. Planting trough; 3. Light source; 4. Temperature sensor; 5. Humidity sensor; 6. Water tank; 7. One planting trough; 8. Another planting trough; 9. Cover plate; 10. Rapeseed stalk passage hole; 11. Rapeseed stalk; 12. Circular cutter blade; 13. Horizontal cutter blade; 14. Horizontal cutting edge; 15. Guide groove; 16. Vertical section; 17. Horizontal extension section; 18. First drive wheel; 19. Second drive wheel; 20. Traction belt; 21. Guide rounded corner; 22. Half section; 23. Hinge; 24. Door latch; 25. Door bolt; 26. Door panel; 27. Crossbeam; 28. Sliding sleeve; 29. ​​Hoist; 30. Hook; 31. Lifting rope; 32. Lifting ring; 33. Supporting protrusion; 34. Positioning groove; 35. Base frame; 36. Vertical cutting edge; 37. Water supply pipe; 38. Liquid level switch; 39. Photosynthesis device; 40. Detailed Implementation

[0018] The technical solutions of the present invention will be described in detail below with reference to the accompanying drawings, but not all of them. Based on the embodiments of the present invention, the present invention will be clearly and completely described. Obviously, the described embodiments are merely a part of the present invention. All other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0019] See Figures 1 to 8An experimental apparatus for evaluating rapeseed's tolerance to moisture damage includes two planting boxes 1, planting troughs 2 within each planting box, a light source 3, a temperature sensor 4, an air conditioning system, a humidity sensor 5, a humidity regulator, and a water supply system. The water supply system includes a water tank 6 and a water pipe 38 connected to the planting trough. The method for evaluating rapeseed's tolerance to moisture damage, using a photosynthesis unit 40, is as follows: rapeseed is planted in both planting troughs. Light is provided to the rapeseed in the planting troughs via the light source. The air conditioning system adjusts the temperature in the planting box to a set value. The temperature sensor detects the temperature in the planting box, the humidity sensor detects the humidity in the planting box, the humidity regulator adjusts the humidity in the planting box to a set value, and the water supply system supplies water to the rapeseed in the planting troughs. The temperature, humidity, and light intensity of the two planting boxes are kept the same. The rapeseed in one planting trough 7 is kept at 3-4... During the leaf stage, the soil was submerged to a depth of 1-2 cm for wet-prone planting until the end of the experiment (the water level was controlled by setting a level switch 39 in the planting trough to control the opening and closing of the water tank's outlet valve). Another planting trough 8 provided a normal growing environment for healthy planting. On the fifth and sixth days of the flooding, the stomatal conductance of both planting troughs was measured using a photosynthesis instrument 40 to obtain the average stomatal conductance Gs1 for wet-prone rapeseed and the average stomatal conductance Gs2 for healthy rapeseed. The moisture tolerance coefficient M was calculated using the formula M=Gs1 / Gs2, and the moisture tolerance of the rapeseed was graded and evaluated: M≥70% was highly moisture-tolerant, 55%≤M<70% was moisture-tolerant, 40%≤M<55% was moderate, and M<40% was sensitive. Two cover plates 9, distributed along the width of the planting trough, are used to seal it. Between the two cover plates are several rapeseed stalk passage holes 10, distributed along the length of the planting trough when the cover plates are closed, allowing the rapeseed stalks in the planting trough to pass through one-to-one. The diameter of the rapeseed stalk passage holes is less than 2 cm larger than the diameter of the rapeseed stalks 11. At the end of the experiment, the rapeseed is removed by closing the two cover plates and then pulling it out of the soil in the planting trough. The rapeseed roots are restricted when passing through the rapeseed stalk passage holes, causing the soil attached to the roots to be scraped off, thus minimizing the amount of soil removed during removal and facilitating a second experiment. When planting rapeseed, it is aligned with the rapeseed stalk passage holes. A downward-facing annular cutting blade 12 is provided at the lower end of the rapeseed stalk passage holes, allowing for easier scraping of the soil attached to the roots. When the two cover plates are closed, the side where the cover plates are joined is higher than the other side. The planting trough is equipped with a soil-loosening mechanism to loosen the soil within it.The soil loosening mechanism includes a transverse cutting strip 13 extending along the width of the planting trough and a vertical cutting strip 14 connected at its lower end to the transverse cutting strip. The transverse cutting strip has transversely extending horizontal cutting edges 15 on both its front and rear sides, and the vertical cutting strip has vertically extending vertical cutting edges 37 on both its front and rear sides. The width of the planting trough is equal from top to bottom. A guide groove 16 is provided on the inner surface of the sidewall in the width direction of the planting trough. The two ends of the transverse cutting strip pass through the guide grooves on the two sidewalls in the width direction of the planting trough. The guide groove includes several vertical segments 17 and several horizontally extending segments 18 extending in the front-rear direction and distributed vertically. Adjacent three... The horizontal extension section has one end connected to one end of the upper horizontal extension section via a vertical section, and the other end connected to one end of the lower horizontal extension section via a vertical section. A first drive wheel 19 is located at the lower end of the guide groove, and a second drive wheel 20 is located at the upper end. The second drive wheel is connected to a drive motor. One end of a traction belt 21 is connected to a horizontal blade, and the other end passes over the first and second drive wheels and connects to the horizontal blade. The traction belt passes through the guide groove, and the length of the vertical blade is greater than the distance between two adjacent horizontal extension ends. The drive motor drives the traction belt in conjunction with the guide groove to drive the horizontal and vertical cutting blades to move horizontally and vertically, thereby ensuring that all the soil in the planting box is tilled. The upper and lower ends of the inner wall of the vertical channel are provided with guide rounded corners 22, which are metal structures. The system also includes a planting trough displacement structure. The planting trough, used to simulate a wetland environment for wetland planting, is equipped with a water level control structure to control the water level of the submerged soil in the planting trough at a set height. The bottom wall of the planting trough is composed of two horizontally distributed halves 23. The outer sides of the two halves are hinged to the planting box body by hinges 24. The lower right surface is provided with two longitudinally distributed door latches 25. The door latches on the two halves are aligned one to one. The two aligned door latches are fitted onto a door latch 26 so that the bottom wall of the planting trough is kept in the whole that closes the lower end of the planting trough body. The top wall of the planting box is provided with an openable door panel 27 aligned with the planting trough. The planting box displacement structure is used to move one planting box to be located directly above another planting box. After tilling, the planting trough is moved so that one planting trough is above another. Then, the latch of the upper planting trough is pulled out, causing the soil in the upper planting trough to fall into the lower planting trough. The latch is then reinserted, closing the bottom wall of the upper planting trough and moving both planting troughs to their initial positions. Then, another planting trough is moved to be above one of the planting troughs. The latch of the upper planting trough is pulled out, causing half of the soil in the upper planting trough to fall into the lower planting trough. The latch is then reinserted, closing the bottom wall of the upper planting trough, thus achieving soil rotation. A second experiment is then conducted on the same rapeseed.The planting box relocation structure includes a crossbeam 28 located above two planting boxes, a sliding sleeve 29 fitted onto the crossbeam, a hoist 30 connected to the sliding sleeve, and a sling 32 with a hook 31 connected to the hoist. The planting trough has lifting rings 33 on both sides that cooperate with the hooks. In use, the hooks are hooked onto the lifting rings of one planting box, the hoist lifts one planting box higher than the other, and then the sliding sleeve moves on the crossbeam so that one planting box is directly above the other, opening the bottom wall of one planting box to allow soil to fall into it. The planting trough is long and narrow, with only one row of rapeseed planted inside. The lower end of the planting trough has a support ridge 34, and the cover plate has a positioning groove 35 for a latch to pass through. The planting trough is supported on a base frame 36.

[0020] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the invention. Although the invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. An experimental apparatus for rapeseed's tolerance to moisture damage, characterized in that, The experiment includes two planting boxes, each equipped with a photosynthesis unit, planting troughs, a light source, a temperature sensor, an air conditioning system, a humidity sensor, a humidity regulator, and a water supply system. The method for evaluating rapeseed's tolerance to waterlogging is as follows: Rapeseed is planted in both planting troughs. Light is provided to the rapeseed in the planting troughs via the light source. The air conditioning system adjusts the temperature in the planting boxes to a set value. The temperature sensor detects the temperature in the planting boxes, the humidity sensor detects the humidity in the planting boxes, the humidity regulator adjusts the humidity in the planting boxes to a set value, and the water supply system supplies water to the rapeseed in the planting troughs. The temperature, humidity, and light are kept identical in both planting boxes. In one planting trough, the rapeseed is submerged in water to a depth of 1-2 cm during the 3-4 leaf stage for waterlogging-induced growth until the end of the experiment. The other planting trough is provided with a normal growing environment for healthy growth. On the fifth and sixth days after submersion, stomatal conductance is measured in both planting boxes using the photosynthesis unit. The average stomatal conductance Gs1 for the waterlogged rapeseed and the average stomatal conductance Gs2 for the healthy rapeseed are obtained over two days. The results are calculated using the formula M = Gs1 / Gs2 is used to calculate the moisture tolerance coefficient M and to classify and evaluate the moisture tolerance of rapeseed varieties: M ≥ 70% is high moisture tolerance, 55% ≤ M < 70% is moisture tolerance, 40% ≤ M < 55% is general tolerance, and M < 40% is sensitive tolerance.

2. The experimental apparatus for rapeseed's tolerance to moisture damage according to claim 1, characterized in that, The planting trough has two cover plates distributed along its width for sealing the trough. Between the two cover plates are several rapeseed stalk passage holes distributed along the length of the planting trough when the cover plates are closed, allowing the rapeseed stalks in the planting trough to pass through one-to-one. The diameter of the rapeseed stalk passage holes is less than 2 cm larger than the diameter of the rapeseed stalks. At the end of the experiment, the rapeseed is removed by closing the two cover plates and then pulling the rapeseed out of the soil in the planting trough. When the rapeseed roots pass through the rapeseed stalk passage holes, the soil attached to the roots is scraped off, thus reducing the amount of soil removed when the rapeseed is removed, which facilitates a second experiment.

3. The experimental apparatus for rapeseed's tolerance to moisture damage according to claim 2, characterized in that, The lower end of the rapeseed stalk through the hole is provided with a downward-facing annular cutting blade.

4. The experimental apparatus for rapeseed's tolerance to moisture damage according to claim 3, characterized in that, When the two cover plates are closed, the side where the cover plates are connected is higher than the other side.

5. The experimental apparatus for rapeseed's tolerance to moisture damage according to claim 1, characterized in that, The planting trough is equipped with a soil loosening mechanism to loosen the soil inside the planting trough.

6. The experimental apparatus for rapeseed's tolerance to moisture damage according to claim 5, characterized in that, The soil loosening mechanism includes a transverse cutting strip extending along the width of the planting trough and a vertical cutting strip connected at its lower end to the transverse cutting strip. The transverse cutting strip has transversely extending horizontal cutting edges on both its front and rear sides, and the vertical cutting strip has vertically extending vertical cutting edges on both its front and rear sides. The width of the planting trough is equal from top to bottom. A guide groove is provided on the inner surface of the sidewall of the planting trough along its width. Both ends of the transverse cutting strip pass through the guide grooves on the two sidewalls of the planting trough along its width. The guide groove includes several vertical segments and several horizontally extending segments distributed in the vertical direction along the front-to-back direction; three adjacent horizontally extending segments... The extension section has one end connected to one end of the upper horizontal extension section via a vertical section, and the other end connected to one end of the lower horizontal extension section via a vertical section. The guide groove has a first drive wheel at the lower end and a second drive wheel at the upper end. The second drive wheel is connected to a drive motor. One end of the traction belt is connected to the transverse blade, and the other end passes around the first and second drive wheels and is connected to the transverse blade. The traction belt passes through the guide groove. The length of the vertical blade is greater than the distance between two adjacent horizontal extension ends.

7. The experimental apparatus for rapeseed's tolerance to moisture damage according to claim 6, characterized in that, The upper and lower ends of the inner wall of the vertical channel are provided with guide rounded corners, which are metal structures.

8. The experimental apparatus for rapeseed's tolerance to moisture damage according to claim 6 or 7, characterized in that, It also includes a planting trough displacement structure. The planting trough, used to simulate a wetland environment for wetland planting, is equipped with a water level control structure. The water level control structure is used to control the water level of the submerged soil in the planting trough at a set height. The bottom wall of the planting trough is composed of two horizontally distributed halves. On the outer side of the two halves, hinged to the planting box body, the lower right side is provided with two longitudinally distributed door latches. The door latches on the two halves are aligned one-to-one. The two aligned door latches are fitted onto a latch so that the bottom wall of the planting trough is kept in the whole that closes the lower end of the planting trough body. The top wall of the planting box is provided with an openable door panel aligned with the planting trough. The planting box displacement structure is used to move one planting box to be located directly above another planting box.

9. The experimental apparatus for rapeseed's tolerance to moisture damage according to claim 8, characterized in that, The planting box displacement structure includes a crossbeam located above the two planting boxes, a sliding sleeve fitted on the crossbeam, a hoist connected to the sliding sleeve, and a lifting cable with a hook connected to the hoist. The planting trough has lifting rings on both sides that cooperate with the hooks.

10. The experimental apparatus for rapeseed's tolerance to moisture damage according to claim 8, characterized in that, The planting trough is long and narrow, and only one row of rapeseed is planted in it. The upper end of the planting trough is provided with two cover plates distributed along the width of the planting trough for sealing the planting trough. Between the two cover plates are several rapeseed stalk passage holes distributed along the length of the planting trough when the cover plates are closed, allowing the rapeseed stalks in the planting trough to pass through one by one. The diameter of the rapeseed stalk passage holes is less than 2 cm larger than the diameter of the rapeseed stalks. The lower end of the planting trough is provided with a support protrusion, and the cover plate is provided with a positioning groove for the door latch to pass through.

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