A method for artificial propagation of an oriental paddlefish

Abstract

This invention discloses an artificial breeding method for the Oriental blackfish, belonging to the field of fish artificial breeding technology. It includes six core steps: broodstock selection and domestication, enhanced maturation cultivation, broodstock selection and anesthesia, spawning induction, artificial insemination, fertilized egg hatching, and fry rearing. One year in advance, suitable-aged wild Oriental blackfish are selected and domesticated in a flowing water pond. Before breeding, tocopherol is mixed into their feed to enhance gonadal development. High-quality broodstock are selected at a female-to-male ratio of 1:2 and anesthetized. A specific ratio of spawning inducing agent is injected using a two-injection method. Artificial insemination is performed in a standardized fiberglass circular fishpond under controlled water flow conditions. Fertilized eggs are incubated at 26±1℃ for 22 hours, and after hatching, they are transferred to a sterilized micro-flow water pond for rearing. This invention achieves a 100% spawning efficiency, a stable fertilization rate of over 95%, and a hatching rate exceeding 82%. It is simple to operate, cost-effective, and allows for large-scale breeding, providing key technical support for the conservation and population expansion of the Oriental blackfish.

Description

Technical Field

[0001] This invention relates to the field of artificial breeding technology for fish, specifically to an artificial breeding method for the Oriental blackfish. Background Technology

[0002] The Oriental lionfish (Garraorientalis Nichols, 1925) belongs to the genus Garraorientalis in the family Cyprinidae. Commonly known as the lionfish or the broken-nosed fish, it is found only in limited water systems in southern my country, such as the Pearl River and the Min River. Due to its narrow natural distribution, overfishing, and ecological degradation, its wild resources are increasingly depleted. There is an urgent need for systematic conservation research and breakthroughs in artificial breeding techniques to ensure the continuation of its population. Core pathways include broodstock collection, broodstock rearing, selective mature individuals, scientific spawning induction, and the application of integrated artificial insemination and flowing-water hatching technologies to achieve large-scale fry production.

[0003] The artificial breeding of Oriental blackfish currently faces two core problems. On the one hand, the scarcity of parent resources leads to a chain of problems. Wild-caught mature individuals are difficult to obtain and are physically weak, resulting in insufficient genetic diversity and an increased risk of inbreeding depression in the breeding population. This not only restricts the viability of offspring but also limits the scope for selective breeding optimization and technological improvement. On the other hand, the technology of the entire breeding chain is immature. Insufficient understanding of the compatibility of spawning stimulants and reproductive ecological preferences (water flow, substrate, etc.) leads to unstable spawning effects. The lack of steps such as sperm motility assessment and optimization of fertilization conditions affects the fertilization rate. Furthermore, the research on the biological characteristics and nutritional requirements of early stages such as embryonic development and larval feeding is weak, further leading to low hatching and survival rates. Key technical challenges such as the selection of first feed and the adaptation of nutritional formulas have not yet been overcome.

[0004] In summary, the scarcity of parent stock and the uncertainties in the technology of each stage of breeding constitute the main bottlenecks in the conservation and artificial propagation of Oriental blackfish, directly hindering the realization of the goal of large-scale production of fry. Therefore, it is urgent to conduct targeted and systematic research to overcome these technical obstacles. Developing an artificial breeding method adapted to the biological characteristics of Oriental blackfish is a key path to solving the aforementioned core problems and promoting species conservation and population expansion. Therefore, this paper proposes an artificial breeding method for Oriental blackfish to address these issues. Summary of the Invention

[0005] In view of this, the technical problem to be solved by the present invention is to propose an efficient artificial breeding technology for Oriental blackfish with high fertilization rate and high membrane emergence rate.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a method for artificial breeding of the Oriental squid, comprising:

[0007] S1, Selection and domestication of breeding fish: One year in advance, select suitable-aged wild Oriental blackhead fish weighing 50-80g and place them in an area of ​​70-80m². 2The broodstock were domesticated and fed in a flowing water breeding pond with a water depth of 1.5-2m. They were fed sturgeon feed with a protein content of 40%-50% and the daily feeding amount was 23% of the fish's body weight. They were fed twice a day, once in the morning and once in the evening. S2, Enhanced maturation of broodstock: One month before breeding, tocopherol is mixed into the feed and fed continuously for one month to promote the development of the gonads of the broodstock. S3, Selection and anesthesia of breeding fish: Select male and female fish. The selection criteria for male fish are a rough snout, a granular body, small water droplets on the pectoral fins with a frosty texture, and bright body color. When the base of the pelvic fin is gently squeezed, white semen will flow out. The selection criteria for female fish are well-developed ovaries, a swollen abdomen, a soft lower abdomen, and a reddish cloaca. After selection at a female-to-male ratio of 1:2, anesthesia is performed. S4, spawning induction of broodstock: The syringe is used to inject the spawning agent into the abdominal cavity at a 45° angle into the depression behind the base of the pectoral fin of the broodstock. After the spawning agent is injected, the broodstock is placed in the spawning tank. S5, Artificial Insemination: The spawning tank is a circular fiberglass fish pond with a diameter of 1.5m. A duckbill-shaped water-pushing device is installed at the water outlet to control the flow rate at 0.5L / s~1L / s. Insemination is completed in 3.5 hours when the parent fish reach their estrus climax and spawn, forming fertilized eggs. S6, Fertilized egg hatching and fry rearing: Collect fertilized eggs, weigh and sample them to calculate the quantity, and place them in a drifting egg incubator. Incubate for 24 hours under sealed pressure pad ±1℃ conditions. After hatching, collect the fry with a 100-mesh net and transfer them to a 20×5×1m micro-flow cement pond that has been disinfected and sterilized in advance for rearing. Preferably, the water temperature during the spawning and hatching process is controlled at 26~29±0.5℃. The spawning induced agent is prepared by human chorionic gonadotropin, ovulation-inducing hormone No. 3, maleate dioxin and sodium chloride solution with a mass fraction of 0.7%. The dosage is HCG 500IU / kg, LRH-A 33ug / kg and DOM 5mg / kg, based on the fish's body weight. The injection volume for female fish is 2mL / kg, and the injection volume for male fish is halved. A two-injection method is used, with the second injection given 3 hours after the first injection.

[0008] Preferably, the fiberglass circular fish pond in S5 includes a water frame, a water tank installed in the water frame, an outlet pipe fixedly connected to the bottom outer surface of the water tank, a booster pump installed at the end of the outlet pipe away from the water tank, an inlet pipe installed on the booster pump, a purification box set below the inlet pipe, and the fiberglass circular fish pond also includes a quick installation mechanism and an adjustable mechanism. The quick-installation mechanism is installed on the water tank and is used for multi-size adjustable installation of the purification box. The adjustable mechanism is installed on the water inlet pipe, which is used for water outlet adjustment.

[0009] Preferably, the quick installation mechanism includes a grooved plate, which is slidably installed on the upper surface of the water tank. An elastic telescopic plate is symmetrically fixedly installed in the middle of the grooved plate. An L-shaped fixing plate is fixedly installed at the end of the elastic telescopic plate away from the grooved plate. The L-shaped fixing plate is fixedly installed on the upper surface of the water tank. The purification box is installed on the upper surface of the grooved plate and the L-shaped fixing plate. A filter plate is slidably installed in the middle of the purification box.

[0010] Preferably, a fixed plate is fixedly installed on one side of the purification box, a pull plate is slidably installed in the middle of the fixed plate, a sealing pad is fixedly installed on the lower surface of the pull plate, elastic telescopic columns are symmetrically fixedly installed on the upper surfaces of both ends of the sealing pad, the end of the elastic telescopic column away from the sealing pad is fixedly installed on the fixed plate, a positioning sealing groove is opened on one side of the filter plate, and the sealing pad is engaged in the positioning sealing groove and has good sealing performance.

[0011] Preferably, the adjustable mechanism includes a telescopic sleeve, the upper end of which is fixedly installed on the water inlet pipe, a threaded limiting groove is formed on the outer surface of the lower end of the telescopic sleeve, an adjusting block is fitted on the outer surface of the telescopic sleeve, the adjusting block is threadedly installed in the threaded limiting groove, a guide groove is formed in the middle of the telescopic sleeve, and a slider is slidably installed in the guide groove of the telescopic sleeve.

[0012] Preferably, a bearing is installed in the middle of the slider, and the bearing is slidably installed in the guide groove of the telescopic sleeve. The slider is set on the lower surface of the adjusting block. Pressure rods are rotatably installed at both ends of the lower surface of the slider. An adjusting tube is rotatably installed at the end of the pressure rod away from the slider. A bearing sleeve is fixedly installed on the outer surface of the lower end of the telescopic sleeve. A T-shaped tube is fixedly installed on the outer surface of the bearing sleeve. The adjusting tube is slidably installed on the inner wall of the T-shaped tube.

[0013] Preferably, an auxiliary spring is fitted on the outer surface of the adjusting tube. One end of the auxiliary spring is fixedly installed on the T-shaped tube, and the other end of the auxiliary spring is fixedly installed on the adjusting tube. A sealing gasket is installed at the connection between the adjusting tube and the T-shaped tube, and the sealing gasket has good waterproof performance.

[0014] Compared with the prior art, the artificial breeding method for Oriental squid provided by this invention has the following beneficial effects: 1. A breakthrough improvement in reproductive efficiency; This solution completely solves the core pain points of "ineffective spawning induction and low fertilization rate" in the artificial breeding of Oriental blackfish, achieving reproductive efficiency far exceeding existing technologies. By optimizing broodstock domestication, strengthening cultivation, and precisely inducing spawning, the spawning efficiency reaches 100%, the fertilization rate remains stable at over 95%, and the hatching rate is maintained at over 82%, while existing technologies or comparative studies often result in ineffective breeding with a fertilization rate of 0%. Furthermore, its large-scale application shows significant results; after expanding the parent stock, the egg production can reach 408,000, achieving a leap from "difficult to breed" to "highly efficient large-scale breeding," and significantly improving fry production efficiency.

[0015] 2. Precise technical parameters result in strong breeding stability; Compared to existing technologies where the combination of spawning stimulants and environmental parameters are unclear, this solution clarifies a key technical parameter system adapted to the biological characteristics of the Oriental blackfish. The spawning induction process employs a two-injection method, with a 3-hour interval between injections, and a specific ratio and dosage of LRH-A3, DOM, and HCG. This avoids problems such as poor egg quality and asynchronous estrus caused by single-injection or staggered injection times. In terms of environmental control, key conditions such as water temperature and flow rate are standardized to match its reproductive ecological preferences, effectively solving potential risks such as oxygen deficiency and water mold infection during flowing water incubation, and significantly improving the stability and repeatability of the entire breeding process.

[0016] 3. Parental protection is in place to ensure sustainable breeding; This program emphasizes the protection and optimization of parent fish resources, establishing a scientific broodstock breeding system. Wild broodstock are domesticated one year in advance, and tocopherol is administered to their feed one month before breeding to accelerate maturation. Combined with clearly defined selection criteria for males and females, this ensures the broodstock are robust and have well-developed gonads, avoiding problems such as egg injury and poor egg quality caused by weak broodstock in existing technologies. Simultaneously, a scientific method of intraperitoneal injection at a 45° angle to the base of the pectoral fin is employed to precisely control the injection depth and dosage, reducing the risk of broodstock injury. This ensures both high yield per breeding cycle and maintains the genetic diversity of the breeding population, laying the foundation for long-term sustainable breeding.

[0017] 4. Simple and controllable operation, reducing application threshold and cost; This solution establishes a standardized breeding process with clear and easy-to-understand steps. It requires no complex specialized equipment, only standard fiberglass fish ponds and floating egg incubators, significantly lowering the technical barrier to entry. Compared to existing technologies that suffer from long parameter exploration cycles and high labor costs, this solution boasts a hatching cycle of only 22 hours, a rapid fry production rate, and eliminates the need for additional high energy consumption. It also reduces parent stock loss and environmental pollution risks, effectively controlling labor and material costs while ensuring breeding success, thus balancing economic benefits and practicality.

[0018] 5. It has outstanding ecological protection value and contributes to species conservation; In response to the increasingly depleted wild resources of the Oriental blackfish, this plan provides crucial technical support for species conservation through breakthroughs in large-scale artificial breeding technology. Large-scale application can stably produce a large number of fry, meeting the needs of restocking and effectively alleviating the population crisis caused by its narrow natural distribution, overfishing, and ecological degradation. Compared to the limitations of existing technologies in forming sustainable breeding populations, this plan achieves a closed loop of "artificial conservation – population expansion – ecological restoration," which is of significant ecological importance for maintaining aquatic biodiversity in the Pearl River and Minjiang River basins. Attached Figure Description

[0019] Figure 1 This is a three-dimensional schematic diagram of the fiberglass circular fish pond in this invention; Figure 2 This is an auxiliary schematic diagram of the three-dimensional structure of the fiberglass circular fish pond of the present invention; Figure 3 This is a schematic diagram of the structural connection relationship of the quick installation mechanism of the present invention; Figure 4 For the present invention Figure 3 Enlarged view of point A in the middle; Figure 5 This is a schematic diagram of the connection relationship of the adjustable mechanism structure of the present invention; Figure 6 For the present invention Figure 5 Enlarged view at point B in the middle; Figure 7 This is a schematic diagram of the artificial breeding method for Oriental blackfish according to the present invention.

[0020] In the picture: 1. Water rack; 11. Water tank; 12. Outlet pipe; 13. Inlet pipe; 14. Purification box; 2. Quick installation mechanism; 21. Grooved plate; 22. Elastic telescopic plate; 23. L-shaped fixing plate; 24. Filter plate; 25. Positioning sealing groove; 26. Sealing gasket; 27. Fixing plate; 28. Elastic telescopic column; 29. ​​Pull plate; 3. Adjustable mechanism; 31. Telescopic sleeve; 32. Threaded limiting groove; 33. Adjusting block; 34. Sliding block; 35. Pressure rod; 36. Adjusting tube; 37. T-tube; 38. Bearing sleeve; 39. Auxiliary spring. Detailed Implementation

[0021] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0022] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments.

[0023] Example 1, please refer to Figure 7 As shown: To address the problems mentioned in the technical solutions, this application provides an artificial breeding method for the Oriental squid. Step 1, Selection and breeding of broodstock: One year in advance, select wild Oriental blackhead fish weighing 50-120g for domestication, 80m 2 They are raised in a 2m deep flowing water pool and fed with 48% egg-based Chinese sturgeon feed. The daily feeding amount is 2-3% of the fish's body weight, twice a day, morning and evening. One month before breeding, tocopherol is mixed into the feed to enhance maturity.

[0024] Step 2, selection criteria for breeding fish: Male fish have a rough snout, a granular body, small, frosty spots on their pectoral fins, and vibrant colors; when the base of the pelvic fins is gently squeezed, white semen will flow out. Female fish have well-developed ovaries, a swollen and soft abdomen, and a reddish vent. The female-to-male ratio is 1:2.

[0025] Step 3, Induction of spawning: A two-injection method is used. The spawning induced agent is prepared by LRH-A3 (ovulation stimulant 3), DOM maleate, HCG, and 0.7% sodium chloride solution. The first injection is LRH-A3 3ug / kg, and the second injection is given 3 hours later, DOM 5mg / kg + HCG 500IU / kg. The injection method is intraperitoneal injection at a 45° angle to the depression behind the base of the pectoral fin, with a needle depth of 0.3cm. The injection volume is 2mL / kg for female fish and half that for male fish.

[0026] Step 4, Induction of Birth Environment: The spawning tank is a 1.5m diameter fiberglass circular fish pond with a duckbill-shaped water pushing device, a flow rate of 0.5-1L / s, and a water temperature controlled at 26.2±0.5℃.

[0027] Step 5, Fertilization and Hatching: Sperm and eggs were collected 6.5 hours after injection for artificial insemination. The fertilized eggs were placed in a drifting egg incubator and incubated at 26±1℃ for 22 hours. After hatching, the water fry were collected with a 100-mesh net and transferred to a sterilized 20×5×1m micro-flow cement pond for cultivation.

[0028] Step six, core results: 100% oxytocin efficiency, 98.2% fertilization rate, 82.1% membrane emergence rate, and 10.2w eggs released.

[0029] Example 2 follows the same screening, breeding, spawning induction, fertilization and hatching process as Example 1, but with an expanded parent stock of 40 females and 20 males.

[0030] The core results of Example 2 were 100% induced labor efficiency, 95.1% fertilization rate, 85.2% membrane emergence rate, and 40.8w of eggs produced, which verified the scalability of the protocol.

[0031] Comparative Example 1, 2023.07.05 single needle group; The single-needle injection method was used, with HCG 500 IU / kg + DOM 5 mg / kg or other HCG dosage combinations as the oxytocin. The injection time was from 8:30 am to 2:00 pm, with an effect time of 6 hours. The water temperature was 28.1℃. No tocopherol-enhanced culture was performed, and there was no standardized water flow stimulation.

[0032] This ratio allows for artificial egg collection, but the egg yield is low (91,000-98,000), with a fertilization rate of 0%. The female fish is severely injured, and the eggs cannot absorb water and swell, and are stuck together, making effective fertilization impossible.

[0033] Comparative Example 22024.07.10 Single-needle optimization group; Single injection, DOM 4mg / kg±HCG 500-700IU / kg, effect time 6h, water temperature 27.2℃, no two-injection or tocopherol-enhanced culture were used, and the flow water setup was not standardized.

[0034] The egg production in this comparison was 78,000-103,000, with a fertilization rate of 0%, indicating poor egg quality and an inability to form viable fertilized eggs.

[0035] Comparative Example 3, the staggered injection group on July 10, 2025; The two-injection method was used, but the injection interval was adjusted instead of 3 hours. For example, the interval was 8 hours, from 8:30 am to 4:30 pm or 10 hours, from 8:30 am to 7:30 pm. The total effect time was 9.5 hours or 12.5 hours. Other conditions were the same as in Example 1.

[0036] In this comparative study, the fertilization rate was only 15.2% in the 8-hour interval group and 0% in the 10-hour interval group. There was no valid data on membrane emergence rate, proving that the 3-hour interval between injections is the optimal time parameter.

[0037] The specific implementation data for this plan are as follows: Labor Induction Information Record Form;

[0038] Based on the above experiments, this solution has the following advantages over existing technologies: This technology provides a systematic solution to the pain points of artificial breeding of Oriental blackfish, achieving a significant leap in reproductive efficiency—100% spawning induction efficiency, a stable fertilization rate of over 95% (maximum 98.2%), and a hatching rate exceeding 82%, completely reversing the situation of extremely low fertilization rates or even infertility with existing technologies. Furthermore, by defining the optimal hormone ratio and interval for two injections, and standardizing key environmental parameters such as water temperature (26-29±0.5℃) and flow rate (0.5-1L / s), the technology ensures the accuracy and stability of technical parameters. Simultaneously, this technology emphasizes parent stock protection and sustainable breeding, relying on early domestication, tocopherol-enhanced maturation, and a scientific intraperitoneal injection method at the base of the pectoral fin to improve parent stock health and reduce the risk of damage, thus maintaining population genetic diversity. Its highly standardized process requires only conventional equipment to complete the entire process from screening, enhancement, spawning induction, fertilization, to hatching, offering advantages such as ease of operation, controllable costs, short hatching cycle, and rapid fry production, effectively balancing economic and ecological benefits. Furthermore, this technology enables large-scale breeding and release of Oriental blackfish, overcoming the bottleneck of existing technologies that struggle to form sustainable breeding populations. It provides crucial technical support for alleviating the depletion of wild resources of this species and for carrying out species conservation.

[0039] Example 3, in which the fiberglass circular fishpond used in this solution includes a water frame 1, and a water tank 11 is installed inside the water frame 1. A water outlet pipe 12 is fixedly connected to the bottom outer surface of the water tank 11. A booster pump is installed at the end of the water outlet pipe 12 away from the water tank 11. An inlet pipe 13 is mounted on the booster pump, and a purification tank 14 is located below the inlet pipe 13. This fiberglass circular fishpond also integrates a quick-installation mechanism 2 and an adjustable mechanism 3. The quick-installation mechanism 2 is located on the water tank 11 and is used to achieve multi-size adaptable installation of the purification tank 14. The adjustable mechanism 3 is located on the inlet pipe 13 and is used to adjust the water outlet state.

[0040] The quick-installation mechanism 2 includes a grooved plate 21, which is slidably mounted on the upper surface of the water tank 11. An elastic telescopic plate 22 is symmetrically fixed to the middle of the grooved plate 21. An L-shaped fixing plate 23 is fixedly connected to the end of the elastic telescopic plate 22 away from the grooved plate 21, and the L-shaped fixing plate 23 is fixed to the upper surface of the water tank 11. A purification box 14 is mounted on the upper surface of the grooved plate 21 and the L-shaped fixing plate 23, and a filter plate 24 is slidably installed in the middle of its interior.

[0041] A fixing plate 27 is fixedly installed on one side of the purification box 14, and a pull plate 29 is slidably inserted through the middle of the fixing plate 27. A sealing gasket 26 is fixed on the lower surface of the pull plate 29, and elastic telescopic columns 28 are symmetrically fixed on the upper surfaces of both ends of the sealing gasket 26. The end of the elastic telescopic column 28 away from the sealing gasket 26 is fixed to the fixing plate 27. Correspondingly, a positioning sealing groove 25 is opened on one side of the filter plate 24, and the sealing gasket 26 is engaged in the positioning sealing groove 25, so as to achieve good sealing performance.

[0042] The adjustable mechanism 3 includes a telescopic sleeve 31, the upper end of which is fixedly mounted on the water inlet pipe 13. A threaded limiting groove 32 is formed on the outer surface of the lower end of the telescopic sleeve 31, and an adjusting block 33 is fitted onto the outer surface, with the adjusting block 33 threadedly assembled into the threaded limiting groove 32. A guide groove is formed in the middle of the telescopic sleeve 31, and a slider 34 is slidably installed in the guide groove. A bearing is assembled in the middle of the slider 34, and the bearing is slidably embedded in the guide groove of the telescopic sleeve 31. The slider 34 is located on the lower surface of the adjusting block 33.

[0043] Both ends of the lower surface of the slider 34 are rotatably mounted with pressure rods 35. The end of the pressure rod 35 away from the slider 34 is rotatably connected to the adjusting tube 36. A bearing sleeve 38 is fixedly mounted on the outer surface of the lower end of the telescopic sleeve 31. A T-shaped tube 37 is fixedly assembled on the outer surface of the bearing sleeve 38. The adjusting tube 36 slides against the inner wall of the T-shaped tube 37. An auxiliary spring 39 is sleeved on the outer surface of the adjusting tube 36. One end of the auxiliary spring 39 is fixed to the T-shaped tube 37, and the other end is fixed to the adjusting tube 36. A sealing gasket is installed at the connection between the adjusting tube 36 and the T-shaped tube 37, providing excellent waterproof performance.

[0044] By rotating the adjusting block 33, the slider 34 can slide along the guide groove of the telescopic sleeve 31, and then the extension and retraction of the adjusting pipe 36 within the T-shaped pipe 37 can be adjusted by the pressure rod 35, thereby achieving precise adjustment of the outlet diameter of the adjusting pipe 36. Compared with the existing technology where water flow directly impacts the filter plate 24, this structure can avoid excessive filtration pressure on some filter holes of the filter plate 24, and at the same time reduce water pollution problems caused by filter hole blockage.

[0045] Water is guided by the telescopic sleeve 31, transmitted through the T-shaped pipe 37 and the regulating pipe 36, and then discharged from the regulating pipe 36. Utilizing the rotatable characteristic of the slider 34, when water impacts the T-shaped pipe 37, the impact force generated by the inconsistent water flow direction drives the T-shaped pipe 37 to rotate around the bearing sleeve 38. At this time, the impacted water flow, along with the rotation of the regulating pipe 36, is evenly sprayed above the filter plate 24, improving the filtration effect.

[0046] Please refer to the above work process. Figures 1 to 6 .

[0047] It should be noted that, in this document, relational terms such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0048] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A method for the artificial breeding of the Oriental squid, characterized in that, include: S1. Select wild Oriental blackhead fish broodstock weighing 50-120g and feed them 40%-50% egg-based Chinese sturgeon feed in a flowing water pool of 70-80m² with a water depth of 1.5-2m. The daily feeding rate is 2-3%, and the fish are fed in the morning and evening. S2. One month before breeding, mix tocopherol into the feed and feed the broodstock continuously to strengthen maturation and promote gonadal development. S3. Select male and female broodstock that meet the physical and physiological characteristics at a ratio of 1:

2. Male fish should be able to squeeze out semen, and female fish should have a swollen and soft abdomen with a reddish vent. After selection, anesthetize the broodstock. S4. Inject the spawning agent into the abdominal cavity at a 45° angle to the depression behind the base of the pectoral fin of the broodstock, and then transfer it into the spawning tank after injection. S5. In a 1.5m diameter fiberglass circular spawning tank, the water flow rate is adjusted to 0.5~1L / s. Artificial insemination is performed 3.5 hours after the second spawning injection when the parent fish are spawning to obtain fertilized eggs. S6. After counting the fertilized eggs, they were incubated for 22 hours in a drifting egg incubator at 26±1℃. After hatching, the fry were collected with a 100-mesh net and transferred to a disinfected 20×5×1m micro-flow cement pond for fry cultivation.

2. The method for artificial breeding of the Oriental squid according to claim 1, characterized in that, The water temperature during the spawning and hatching process is controlled at 26~29±0.5℃. The spawning induced agent is prepared by human chorionic gonadotropin (HCG), ovulation-inducing hormone (LRH-A3), domperidone maleate (DOM), and a sodium chloride solution with a mass fraction of 0.7%. The dosage is 500 IU / kg HCG, 3 ug / kg LRH-A3, and 5 mg / kg DOM, based on the fish's body weight. The injection dose for female fish is 2 mL / kg, and the injection dose for male fish is halved. A two-injection method is used, with the second injection given 3 hours after the first injection.

3. The method for artificial breeding of the Oriental squid according to claim 1, characterized in that: The fiberglass circular fish pond described in S5 includes a water frame (1), a water tank (11) installed in the water frame (1), an outlet pipe (12) fixedly connected to the bottom outer surface of the water tank (11), a booster pump installed at the end of the outlet pipe (12) away from the water tank (11), an inlet pipe (13) installed on the booster pump, and a purification box (14) set below the inlet pipe (13). The fiberglass circular fish pond also includes a quick installation mechanism (2) and an adjustable mechanism (3). The quick installation mechanism (2) is installed on the water tank (11), and the quick installation mechanism (2) is used for multi-size adjustable installation of the purification box (14); The adjustable mechanism (3) is installed on the water inlet pipe (13), which is used for water outlet adjustment.

4. The method for artificial breeding of the Oriental squid according to claim 3, characterized in that: The quick installation mechanism (2) includes a grooved plate (21), which is slidably installed on the upper surface of the water tank (11). An elastic telescopic plate (22) is symmetrically fixedly installed in the middle of the grooved plate (21). An L-shaped fixing plate (23) is fixedly installed at the end of the elastic telescopic plate (22) away from the grooved plate (21). The L-shaped fixing plate (23) is fixedly installed on the upper surface of the water tank (11). The purification box (14) is installed on the upper surfaces of the grooved plate (21) and the L-shaped fixing plate (23). A filter plate (24) is slidably installed in the middle of the purification box (14).

5. The method for artificial breeding of the Oriental squid according to claim 4, characterized in that: A fixed plate (27) is fixedly installed on one side of the purification box (14). A pull plate (29) is slidably installed in the middle of the fixed plate (27). A sealing pad (26) is fixedly installed on the lower surface of the pull plate (29). Elastic telescopic columns (28) are symmetrically fixedly installed on the upper surfaces of both ends of the sealing pad (26). The end of the elastic telescopic column (28) away from the sealing pad (26) is fixedly installed on the fixed plate (27). A positioning sealing groove (25) is opened on one side of the filter plate (24). The sealing pad (26) is engaged in the positioning sealing groove (25) and has good sealing performance.

6. The method for artificial breeding of the Oriental squid according to claim 5, characterized in that: The adjustable mechanism (3) includes a telescopic sleeve (31), the upper end of which is fixedly installed on the water inlet pipe (13), a threaded limiting groove (32) is provided on the outer surface of the lower end of the telescopic sleeve (31), an adjusting block (33) is fitted on the outer surface of the telescopic sleeve (31), the adjusting block (33) is threadedly installed in the threaded limiting groove (32), a guide groove is provided in the middle of the telescopic sleeve (31), and a slider (34) is slidably installed in the guide groove of the telescopic sleeve (31).

7. The method for artificial breeding of the Oriental squid according to claim 6, characterized in that: A bearing is installed in the middle of the slider (34), and the bearing is slidably installed in the guide groove of the telescopic sleeve (31). The slider (34) is set on the lower surface of the adjusting block (33). Pressure rods (35) are rotatably installed at both ends of the lower surface of the slider (34). An adjusting tube (36) is rotatably installed at the end of the pressure rod (35) away from the slider (34). A bearing sleeve (38) is fixedly installed on the outer surface of the lower end of the telescopic sleeve (31). A T-shaped tube (37) is fixedly installed on the outer surface of the bearing sleeve (38). The adjusting tube (36) is slidably installed on the inner wall of the T-shaped tube (37).

8. The method for artificial breeding of the Oriental squid according to claim 7, characterized in that: An auxiliary spring (39) is fitted on the outer surface of the regulating tube (36). One end of the auxiliary spring (39) is fixedly installed on the T-shaped tube (37), and the other end of the auxiliary spring (39) is fixedly installed on the regulating tube (36). A sealing gasket is installed at the connection between the regulating tube (36) and the T-shaped tube (37), and the sealing gasket has good waterproof performance.

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