Automatic animal serum separation and dispensing device

Abstract

This invention provides an automated animal serum separation and dispensing device, comprising an outer casing with a top cover, a vacuum tube placement tray inside the casing, a serum collection mechanism within the casing, a serum extraction and transfer mechanism, and a cleaning and disinfection mechanism within the casing. It integrates centrifugation, intelligent extraction, precise dispensing, and tubing disinfection functions. A serum extraction reversing frame drives the extraction needle to achieve precise serum extraction. A serum level monitoring probe enables closed-loop control of the dispensing volume. A rapid biochemical indicator detection panel can simultaneously screen for hemolysis and protein abnormalities. After the operation is completed, the cleaning and disinfection process automatically starts to avoid batch cross-contamination. This invention achieves full automation of the serum processing process, significantly improving efficiency, accuracy, and safety, and is suitable for various scenarios such as laboratory and on-site testing.

Description

Technical Field

[0001] This invention relates to the field of animal serum separation technology, and more specifically, to an automated animal serum separation and dispensing device. Background Technology

[0002] Animal serum is a widely used biological raw material in biopharmaceutical research and development, vaccine preparation, cell culture, and clinical testing. Its purity, homogeneity, and lack of contamination directly determine the reliability of subsequent experiments and production activities. In large-scale biopharmaceutical production and scientific research experiments, it is often necessary to process hundreds or even thousands of animal blood samples simultaneously. Efficient and safe serum separation and packaging are key steps in the entire preparation process.

[0003] Traditional serum preparation processes are highly dependent on manual operation and generally involve the following steps: First, the collected anticoagulated blood sample is placed in a centrifuge for centrifugation to separate the serum and blood cells. After the serum and blood cells have separated at the interface, the experimenter manually aspirates the upper serum layer using a pipette and then dispenses it dropwise into storage tubes. The current process has many shortcomings and needs improvement. Manual single-pipettes can only aspirate and dispense a single sample at a time. When faced with large-scale preparation orders, processing a single batch of samples requires a lot of manpower and time, which is difficult to adapt to the high-throughput preparation needs of modern biotechnology industry, resulting in extremely high labor and time costs.

[0004] During manual operation, pipette tips are prone to contact with external environments such as sample tube walls and operating tables. In addition, incomplete pipette switching between different samples and hand contact by operators can easily cause external contamination and cross-contamination of samples, which can seriously damage the purity of serum, leading to distorted experimental data or even the scrapping of production batches.

[0005] When manually aspirating serum, the depth of pipette application and the aspiration speed rely entirely on the operator's experience. This can easily lead to problems such as aspirating too deeply, disturbing the blood cell layer and mixing serum with blood cell impurities, or aspirating too little, reducing sample utilization and ultimately resulting in inconsistent quality within the same batch of serum products. Therefore, we have made improvements and proposed an automated animal serum separation and dispensing device. Summary of the Invention

[0006] The purpose of this invention is to address the problems raised in the existing background technology. To achieve the above-mentioned objective, this invention provides the following technical solution: an automatic animal serum separation and dispensing device, comprising a device housing, a top cover plate on the top of the device housing, a vacuum tube placement tray inside the device housing, and a vacuum tube placement hole provided in the vacuum tube placement tray; The vacuum tube placement tray is connected to the placement tray frame, and a sealed enclosure is provided around the vacuum tube placement tray. A serum collection mechanism is provided inside the outer shell of the device, and the serum collection mechanism is equipped with a serum extraction and transfer mechanism. A cleaning and disinfection mechanism is provided inside the outer shell of the device.

[0007] As a preferred embodiment of the present invention, the serum collection mechanism includes a serum extraction needle, which is connected to a delivery tube.

[0008] As a preferred technical solution of the present invention, the serum extraction and transfer mechanism includes a serum extraction reversing frame, and the serum extraction reversing frame is connected to a steering motor via an extraction reversing shaft.

[0009] As a preferred technical solution of the present invention, the cleaning and disinfection mechanism includes a rinsing and disinfection spray pipe, the rinsing and disinfection spray pipe is equipped with a rinsing and disinfection nozzle, and the rinsing and disinfection spray pipe is connected to a cleaning and disinfection solution injection pump through a pipeline.

[0010] As a preferred technical solution of the present invention, a serum level monitoring probe is provided on the side of the vacuum tube placement hole, and a rapid detection plate for serum biochemical indicators is provided on the side of the serum extraction needle.

[0011] As a preferred embodiment of the present invention, a control switch is provided on the side of the outer casing of the device, and a centrifugal force field dynamic balancing turntable is provided inside the outer casing of the device.

[0012] As a preferred technical solution of the present invention, a multi-channel divergent partition plate is provided on the outer side of the centrifugal force field dynamic balancing turntable, and an anti-vibration rotor raft is connected to the upper part of the centrifugal force field dynamic balancing turntable.

[0013] As a preferred technical solution of the present invention, a heat dissipation grid is provided on the lower inner side of the device housing, a storage battery is provided inside the device housing, casters are installed at the bottom of the device housing, and an adjustment support pad is also provided at the bottom of the device housing.

[0014] The dispensing method of an automated animal serum separation and dispensing device includes the following steps; Step 1: Preliminary preparation and debugging of the device: Adjust the outer shell of the device to a horizontal and stable state by adjusting the support rubber pads, close the locking structure of the universal wheels, press the control switch to start the device, and power it by the battery; Place the vacuum tubes to be packaged into the vacuum tube placement holes of the vacuum tube placement tray, and initially seal the area of ​​the vacuum tube placement tray with a sealing barrier to prevent external contamination. Start the cleaning and disinfection mechanism: The cleaning and disinfection solution is delivered to the rinsing and disinfection spray nozzle by the cleaning and disinfection solution filling pump. The serum extraction needle, delivery tubing and vacuum tube placement tray are thoroughly rinsed and disinfected through the rinsing and disinfection nozzle. After completion, the waste liquid is discharged. Step 2: Serum extraction and transport preparation: The serum extraction and transfer mechanism is started, and the steering motor drives the serum extraction reversing frame to rotate through the extraction reversing shaft, adjusting the serum extraction needle to the sample position of the serum to be extracted and aligning it with the sample container. The serum collection mechanism is activated, and the serum extraction needle is inserted into the sample container to extract the serum through negative pressure. The serum is then transported into the device through the delivery tubing. During the transport process, the serum flows through the rapid detection plate for serum biochemical indicators to preliminarily detect the basic biochemical indicators of the serum and confirm that the serum meets the dispensing standards. Step 3: Centrifugation: The extracted serum is transferred to the dynamic equilibrium rotating disk area of ​​the centrifugal force field; The vibration acceleration of the centrifugal force field dynamic equilibrium turntable satisfies: in: The maximum vibration acceleration of the centrifugal force field dynamic equilibrium turntable; This is the distance from the center of the turntable to the serum placement position; This is the stiffness coefficient of the vibration-resistant rotor raft; This is the initial eccentricity of the turntable; The total mass of the turntable and serum; The natural vibration angular frequency of the vibration-resistant rotor raft; Multi-channel divergent separators divert serum to different centrifugation channels, and anti-vibration rotor rafts buffer vibrations during centrifugation. The centrifugal force field dynamic balance turntable is started, which drives the serum to rotate at high speed. Centrifugal force is used to separate the serum from blood cells and other impurities. During the separation process, the heat generated by centrifugation is dissipated through the heat dissipation grid to maintain a stable internal temperature of the device. The dynamic balance correction formula for the vacuum tube placement tray is: in: The unbalanced force that needs to be corrected in real time for the vacuum tube placement tray; For the first The total mass of the vacuum tube and the serum inside; The rotational angular velocity of the turntable in the dynamic equilibrium of the centrifugal force field; For the first The radial mounting radius of each vacuum tube on the vacuum tube placement plate; For the first The circumferential installation angle of each vacuum tube; The average circumferential installation angle for all vacuum tubes; The total number of holes for placing vacuum tubes; Step 4: Precise dispensing: After separation, the upper layer of serum is re-aggregated, and the serum extraction and transfer mechanism readjusts the position of the serum extraction needle, aligning it with the vacuum tube in the vacuum tube placement hole. Serum is injected into a vacuum tube via a serum extraction needle through a delivery tubing, and a serum level monitoring probe monitors the serum level in the vacuum tube in real time. The formula for correcting the level detection error of the serum level monitoring probe is as follows: in: This is the correction value for liquid level detection error; The probe is used to detect the Poisson's ratio of the diaphragm; This represents the actual serum level. To detect the elastic modulus of the diaphragm; To detect the thickness of the membrane; The temperature influence coefficient of the probe material; When the liquid level reaches the preset value, stop adding liquid and complete the dispensing of a single vacuum tube; The formula for calculating the flow rate of the serum extraction needle is as follows: in: Real-time extraction volume flow rate of serum extraction syringe; This refers to the inner diameter of the serum extraction needle. The outer diameter of the metal guidewire inside the syringe; The pressure difference between the two ends of the serum extraction needle; The dynamic viscosity of serum; The effective length of the serum extraction syringe; This refers to the friction coefficient along the pipeline. This is the equivalent length of the delivery pipeline; To measure the density of serum in real time; This is the reference density of serum under standard conditions; The tray holder moves the vacuum tube placement tray step by step, moving the next empty vacuum tube to the filling position. Repeat the above filling steps until all vacuum tubes are filled. As a preferred technical solution of the present invention, it also includes step five: finishing after dispensing: after all vacuum tubes are dispensed, the cleaning and disinfection mechanism is restarted to perform secondary cleaning and disinfection on the serum extraction needle and delivery tubing to avoid cross-contamination; the control switch is turned off, the battery power supply is disconnected, the dispensed vacuum tubes are taken out from the vacuum tube placement hole, the inside of the device is tidied up, and the entire dispensing process is completed.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention integrates the entire process of centrifugation, serum extraction, dispensing, and disinfection into a fully automated operation, replacing the traditional manual method of aspirating serum. It can simultaneously dispense multiple tubes of serum in a single batch, improving serum processing efficiency while avoiding the risk of sample contamination introduced by manual operation, and is suitable for large-scale animal serum preparation scenarios.

[0016] After the operation is completed, the cleaning and disinfection process is automatically started. The high-pressure disinfectant can achieve thorough rinsing of the inner wall of the extraction needle and delivery pipeline without dead corners. Combined with air purging, residual droplets are removed, which completely avoids cross-contamination between different batches of serum samples and ensures serum purity and experimental reliability.

[0017] This invention features a centrifugal force field dynamic balance turntable paired with an anti-vibration rotor raft, which effectively counteracts rotational vibration, reduces sample disturbance during centrifugation, and ensures a clear interface between serum and blood cells. A multi-channel divergent separator, combined with the centrifugal force field dynamic balance structure, ensures stable operation of the device during high-speed rotation, reduces the amplitude of machine vibration, and avoids vibration affecting sensor accuracy and vacuum tube samples. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the structure provided by the present invention; Figure 2 This is a schematic diagram of the internal structure provided by the present invention; Figure 3 This is a schematic diagram of the internal structure provided by the present invention; Figure 4 This is a schematic diagram of the internal structure provided by the present invention; Figure 5 This is a schematic diagram of the internal structure provided by the present invention; Figure 6 This is a partial structural schematic diagram provided by the present invention; Figure 7 This is a side view structural diagram provided by the present invention.

[0019] The image shows: 1. Device outer casing; 2. Top cover plate; 3. Vacuum tube placement tray; 31. Vacuum tube placement hole; 4. Placement tray rack; 5. Sealed enclosure; 6. Serum collection mechanism; 61. Serum extraction needle; 62. Delivery tubing; 7. Serum extraction and transfer mechanism; 71. Serum extraction reversing frame; 72. Extraction reversing shaft; 73. Steering motor; 8. Cleaning and disinfection mechanism; 81. Rinsing disinfection spray nozzle; 82. Rinsing disinfection nozzle; 83. Cleaning and disinfection solution filling pump; 9. Serum level monitoring probe; 10. Serum biochemical index rapid detection plate; 11. Control switch; 12. Centrifugal force field dynamic balance turntable; 13. Multi-channel divergent partition plate; 14. Vibration-resistant rotor raft; 15. Heat dissipation grid; 16. Battery; 17. Casters; 18. Adjustable support pads. Detailed Implementation

[0020] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention.

[0021] Therefore, the following detailed description of the embodiments of the present invention is not intended to limit the scope of the claimed invention, but merely illustrates some embodiments of the invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention. It should be noted that, in the absence of conflict, the embodiments and features and technical solutions in the embodiments of the present invention can be combined with each other. It should be noted that similar reference numerals and letters in the following figures indicate similar items; therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0022] Example 1: An automatic dispensing device for separating animal serum includes a device housing 1, a top cover plate 2 on the top of the device housing 1, a vacuum tube placement tray 3 inside the device housing 1, and a vacuum tube placement hole 31 on the vacuum tube placement tray 3. The vacuum tube placement tray 3 is connected to the placement tray frame 4. A sealing barrier 5 is set around the vacuum tube placement tray 3. A serum collection mechanism 6 is set inside the outer shell 1 of the device. The serum collection mechanism 6 is equipped with a serum extraction and transfer mechanism 7. A cleaning and disinfection mechanism 8 is set inside the outer shell 1 of the device.

[0023] The serum collection mechanism 6 includes a serum extraction needle 61, which is connected to a delivery line 62. The serum extraction and transfer mechanism 7 includes a serum extraction reversing frame 71, which is connected to a steering motor 73 via an extraction reversing shaft 72.

[0024] The cleaning and disinfection mechanism 8 includes a rinsing and disinfection spray pipe 81, which is equipped with a rinsing and disinfection nozzle 82. The rinsing and disinfection spray pipe 81 is connected to a cleaning and disinfection solution filling pump 83 via a pipe. A serum level monitoring probe 9 is installed on the side of the vacuum tube placement hole 31, and a rapid serum biochemical index detection plate 10 is installed on the side of the serum extraction needle tube 61. A control switch 11 is installed on the side of the device housing 1, and a centrifugal force field dynamic balance turntable 12 is installed inside the device housing 1.

[0025] A multi-channel divergent partition plate 13 is provided on the outside of the centrifugal force field dynamic balance turntable 12, and an anti-vibration rotor raft 14 is connected to the upper part of the centrifugal force field dynamic balance turntable 12.

[0026] A heat dissipation grid 15 is provided on the lower inner side of the device housing 1. A battery 16 is installed inside the device housing 1. A caster wheel 17 is installed at the bottom of the device housing 1. An adjustment support pad 18 is also provided at the bottom of the device housing 1. The bottom adjustment support pad can quickly level the equipment and reduce the difficulty of on-site deployment.

[0027] The dispensing method of an automated animal serum separation and dispensing device includes the following steps; Step 1: Preliminary preparation and debugging of the device: Adjust the outer shell 1 of the device to a horizontal and stable state by adjusting the support rubber pad 18, close the locking structure of the universal wheel 17, press the control switch 11 to start the device, and power it from the battery 16. Place the vacuum tubes to be packaged into the vacuum tube placement holes 31 of the vacuum tube placement tray 3, and use the sealing barrier 5 to initially seal the area of ​​the vacuum tube placement tray 3 to prevent external contamination. Start the cleaning and disinfection mechanism 8: The cleaning and disinfection solution is delivered to the rinsing and disinfection spray nozzle 81 by the cleaning and disinfection solution filling pump 83. The serum extraction needle 61, the delivery line 62 and the vacuum tube placement tray 3 are thoroughly rinsed and disinfected through the rinsing and disinfection nozzle 82. After completion, the waste liquid is discharged. Step 2: Serum extraction and transport preparation: The serum extraction and transfer mechanism 7 is started, and the steering motor 73 drives the serum extraction reversing frame 71 to rotate through the extraction reversing shaft 72, adjusting the serum extraction needle 61 to the sample position of the serum to be extracted and aligning it with the sample container. When the serum collection mechanism 6 is activated, the serum extraction needle 61 is inserted into the sample container and the serum is extracted by negative pressure. The serum is then transported into the device through the delivery tube 62. During the transport process, the serum flows through the rapid detection plate 10 for serum biochemical indicators to preliminarily detect the basic biochemical indicators of the serum and confirm that the serum meets the dispensing standards. Step 3: Centrifugation process: The extracted serum is transported to the area of ​​the centrifugal force field dynamic balance turntable 12. The multi-channel divergent separator 13 diverts the serum to different centrifugation channels, and the anti-vibration rotor raft 14 buffers the vibration during the centrifugation process. The centrifugal force field dynamic balance turntable 12 is started, which drives the serum to rotate at high speed. Centrifugal force is used to separate the serum from blood cells and other impurities. During the separation process, the heat generated by centrifugation is dissipated through the heat dissipation grid 15 to maintain the stable internal temperature of the device. Step 4: Precise dispensing: After separation, the upper layer of serum is re-aggregated, and the serum extraction and transfer mechanism 7 readjusts the position of the serum extraction needle 61, aligning it with the vacuum tube in the vacuum tube placement hole 31. Serum is injected into the vacuum tube through the delivery tube 62 and the serum extraction needle 61. The serum level monitoring probe 9 monitors the serum level in the vacuum tube in real time. When the level reaches the preset value, the injection is stopped, and the dispensing of a single vacuum tube is completed. The tray rack 4 moves the vacuum tube placement tray 3 step by step, moving the next empty vacuum tube to the filling position. The above filling steps are repeated until all vacuum tubes are filled. Step 5: Finishing after dispensing: After all vacuum tubes are dispensed, restart the cleaning and disinfection mechanism 8 to perform a second cleaning and disinfection of the serum extraction needle 61 and delivery tubing 62 to avoid cross-contamination; turn off the control switch 11, disconnect the power supply from the battery 16, remove the dispensed vacuum tubes from the vacuum tube placement hole 31, tidy up the inside of the device, and complete the entire dispensing process.

[0028] Example 2: An automatic dispensing device for separating animal serum includes a device housing 1, a top cover plate 2 on the top of the device housing 1, a vacuum tube placement tray 3 inside the device housing 1, and a vacuum tube placement hole 31 on the vacuum tube placement tray 3. The vacuum tube placement tray 3 is connected to the placement tray frame 4. A sealing barrier 5 is set around the vacuum tube placement tray 3. A serum collection mechanism 6 is set inside the outer shell 1 of the device. The serum collection mechanism 6 is equipped with a serum extraction and transfer mechanism 7. A cleaning and disinfection mechanism 8 is set inside the outer shell 1 of the device.

[0029] The serum collection device 6 includes a serum extraction needle 61, which is connected to a delivery tube 62.

[0030] The serum extraction and transfer mechanism 7 includes a serum extraction reversing frame 71, which is connected to a steering motor 73 via an extraction reversing shaft 72.

[0031] The cleaning and disinfection mechanism 8 includes a rinsing and disinfection spray pipe 81, which is equipped with a rinsing and disinfection nozzle 82. The rinsing and disinfection spray pipe 81 is connected to a cleaning and disinfection solution injection pump 83 via a pipeline.

[0032] A serum level monitoring probe 9 is installed on the side of the vacuum tube placement hole 31, and a rapid detection plate 10 for serum biochemical indicators is installed on the side of the serum extraction needle tube 61.

[0033] A control switch 11 is provided on the side of the outer casing 1 of the device, and a centrifugal force field dynamic balancing turntable 12 is provided inside the outer casing 1 of the device.

[0034] A multi-channel divergent partition plate 13 is provided on the outside of the centrifugal force field dynamic balance turntable 12, and an anti-vibration rotor raft 14 is connected to the upper part of the centrifugal force field dynamic balance turntable 12.

[0035] A heat dissipation grid 15 is provided on the lower inner side of the device housing 1, a storage battery 16 is provided inside the device housing 1, casters 17 are installed at the bottom of the device housing 1, and an adjustment support pad 18 is also provided at the bottom of the device housing 1.

[0036] Working Principle: This automated animal serum separation and dispensing device integrates sample centrifugation, serum extraction, dispensing, tubing disinfection, and process monitoring functions to automate the entire process of animal serum processing, dispensing, and storage. The core operating logic is as follows: after sample centrifugation and stratification, the upper serum layer is automatically extracted and precisely dispensed into vacuum tubes. After completion, contact parts are cleaned and disinfected. The entire process is monitored by sensors and controlled by a program to ensure stability and safety.

[0037] A centrifugal dynamic balance turntable 12 rotates at high speed driven by a drive device, and the container holding the animal blood samples to be separated rotates synchronously with the turntable. Utilizing the principle of centrifugal sedimentation, blood cells with higher density move outward under centrifugal force, while serum with lower density accumulates in the upper layer of the sample container, achieving efficient separation of serum and blood cells. A multi-channel divergent separator 13 physically separates each sample station, preventing disturbances and interference between samples during high-speed rotation. An anti-vibration rotor raft 14 uses a flexible buffer structure to counteract the high-frequency vibrations generated by centrifugal rotation, ensuring the dynamic balance of the turntable operation and preventing vibration from affecting the accuracy of serum stratification and the overall stability of the device.

[0038] Positioning and Monitoring: The vacuum tube placement tray 3 accurately positions the empty vacuum tube through the vacuum tube placement hole 31. The serum level monitoring probe 9 collects the liquid level data in the vacuum tube in real time, providing a trigger signal for dispensing to stop. At the same time, it monitors the serum level in the sample container after centrifugation in real time, providing data support for the needle insertion depth of the extraction needle.

[0039] Extraction Operation: The steering motor 73 drives the serum extraction reversing frame 71 to perform angular displacement via the extraction reversing shaft 72, precisely adjusting the spatial posture of the serum extraction needle 61 and delivering the needle into the serum stratification area of ​​the centrifuged sample container. Subsequently, the upper layer of serum is stably extracted through the negative pressure extraction system in conjunction with the delivery tubing 62. The rapid serum biochemical index detection plate 10 simultaneously performs rapid pre-detection on the extracted serum, providing real-time feedback on serum hemolysis, abnormal protein content, etc., and promptly terminating the dispensing process for unqualified samples.

[0040] Precise dispensing: The extracted serum is sent into the empty vacuum tube in the vacuum tube placement hole 31 through the delivery tube 62. When the serum level monitoring probe 9 detects that the vacuum tube has reached the preset dispensing capacity, the system automatically stops the serum delivery, completes the single tube dispensing, and then the needle is removed to switch the station for the next tube dispensing operation.

[0041] After a single dispensing process is completed, the cleaning and disinfectant dispensing pump 83 starts, pressurizing and delivering the cleaning and disinfectant solution to the rinsing and disinfection spray nozzle 81. The high-pressure disinfectant solution is then sprayed through the rinsing and disinfection nozzle 82 onto the inner wall of the serum extraction needle 61, the inner wall of the delivery tubing 62, and the residual splash area on the vacuum tube placement tray. After the disinfectant solution has been used for a preset soaking and rinsing time, clean air is introduced into the system to purge the tubing and needles, completing a comprehensive cleaning and disinfection of the extraction path and preventing cross-contamination between batches of samples.

[0042] Heat dissipation system: During the operation of the device, the heat generated by the motor, centrifugal turntable and filling pump is quickly discharged to the outside of the casing through the heat dissipation grid 15. Combined with the internal air circulation, it ensures that the operating temperature of electronic components and power components is within a safe range.

[0043] Mobility and support: Battery 16 provides power support for the device in scenarios where there is no external power source; casters 17 enable the device to move flexibly. After arriving at the work position, the support pads 18 are adjusted to extend downwards to contact the ground, locking the device position and preventing displacement of the device due to operating vibration.

[0044] The dispensing method of an automated animal serum separation and dispensing device includes the following steps; Step 1: Preliminary preparation and debugging of the device: Adjust the outer shell 1 of the device to a horizontal and stable state by adjusting the support rubber pad 18, close the locking structure of the universal wheel 17, press the control switch 11 to start the device, and power it from the battery 16. Place the vacuum tubes to be packaged into the vacuum tube placement holes 31 of the vacuum tube placement tray 3, and use the sealing barrier 5 to initially seal the area of ​​the vacuum tube placement tray 3 to prevent external contamination. Start the cleaning and disinfection mechanism 8: The cleaning and disinfection solution is delivered to the rinsing and disinfection spray nozzle 81 by the cleaning and disinfection solution filling pump 83. The serum extraction needle 61, the delivery line 62 and the vacuum tube placement tray 3 are thoroughly rinsed and disinfected through the rinsing and disinfection nozzle 82. After completion, the waste liquid is discharged. Step 2: Serum extraction and transport preparation: The serum extraction and transfer mechanism 7 is started, and the steering motor 73 drives the serum extraction reversing frame 71 to rotate through the extraction reversing shaft 72, adjusting the serum extraction needle 61 to the sample position of the serum to be extracted and aligning it with the sample container. When the serum collection mechanism 6 is activated, the serum extraction needle 61 is inserted into the sample container and the serum is extracted by negative pressure. The serum is then transported into the device through the delivery tube 62. During the transport process, the serum flows through the rapid detection plate 10 for serum biochemical indicators to preliminarily detect the basic biochemical indicators of the serum and confirm that the serum meets the dispensing standards. Step 3: Centrifugation process: The extracted serum is transported to the dynamic equilibrium rotating disk 12 of the centrifugal force field; The vibration acceleration of the centrifugal force field dynamic equilibrium turntable 12 satisfies: in: The maximum vibration acceleration of the centrifugal force field dynamic equilibrium turntable 12; This is the distance from the center of the turntable to the serum placement position; The stiffness coefficient of the vibration-resistant rotor raft 14; This is the initial eccentricity of the turntable; The total mass of the turntable and serum; The natural vibration angular frequency of the vibration-resistant rotor raft 14; The multi-channel divergent separator 13 diverts serum to different centrifugation channels, and the anti-vibration rotor raft 14 buffers vibrations during centrifugation. The centrifugal force field dynamic balance turntable 12 is started, which drives the serum to rotate at high speed. Centrifugal force is used to separate the serum from blood cells and other impurities. During the separation process, the heat generated by centrifugation is dissipated through the heat dissipation grid 15 to maintain the stable internal temperature of the device. The dynamic balance correction formula for the vacuum tube placement tray 3 is as follows: in: The unbalanced force that needs to be corrected in real time for the vacuum tube placement disk 3; For the first The total mass of the vacuum tube and the serum inside; The rotational angular velocity of the centrifugal force field dynamic equilibrium turntable 12; For the first The radial mounting radius of each vacuum tube on the vacuum tube placement plate 3; For the first The circumferential installation angle of each vacuum tube; The average circumferential installation angle for all vacuum tubes; The total number of vacuum tube placement holes 31; Step 4: Precise dispensing: After separation, the upper layer of serum is re-aggregated, and the serum extraction and transfer mechanism 7 readjusts the position of the serum extraction needle 61, aligning it with the vacuum tube in the vacuum tube placement hole 31. Serum is injected into a vacuum tube via delivery tubing 62 and serum extraction needle 61, and serum level monitoring probe 9 monitors the serum level in the vacuum tube in real time. The formula for correcting the level detection error of the serum level monitoring probe 9 is as follows: in: This is the correction value for liquid level detection error; The probe is used to detect the Poisson's ratio of the diaphragm; This represents the actual serum level. To detect the elastic modulus of the diaphragm; To detect the thickness of the membrane; The temperature influence coefficient of the probe material; When the liquid level reaches the preset value, stop adding liquid and complete the dispensing of a single vacuum tube; The formula for calculating the extraction flow rate of the serum extraction needle 61 is as follows: in: Real-time extraction volume flow rate for serum extraction syringe 61; The inner diameter of the serum extraction needle 61; The outer diameter of the metal guidewire inside the syringe; The pressure difference between the two ends of the serum extraction needle 61; The dynamic viscosity of serum; The effective length of the serum extraction syringe 61; The friction factor of pipeline 62; This is the equivalent length of the delivery pipeline 62; To measure the density of serum in real time; This is the reference density of serum under standard conditions; The tray rack 4 moves the vacuum tube placement tray 3 step by step, moving the next empty vacuum tube to the filling position. The above filling steps are repeated until all vacuum tubes are filled. The process also includes step five: finishing after dispensing: after all vacuum tubes are dispensed, the cleaning and disinfection mechanism 8 is restarted to perform secondary cleaning and disinfection on the serum extraction needle 61 and the delivery line 62 to avoid cross-contamination; the control switch 11 is turned off, the power supply of the battery 16 is disconnected, the dispensed vacuum tubes are taken out from the vacuum tube placement hole 31, the inside of the device is cleaned up, and the entire dispensing process is completed.

[0045] The above embodiments are only used to illustrate the present invention and are not intended to limit the technical solutions described herein. Although the present invention has been described in detail with reference to the above embodiments, the present invention is not limited to the specific embodiments described above. Therefore, any modifications or equivalent substitutions to the present invention, as well as all technical solutions and improvements that do not depart from the spirit and scope of the invention, are covered within the scope of the claims of the present invention.

Claims

1. An automated dispensing device for separating and packaging animal serum, characterized in that, The device includes a housing (1), a top cover plate (2) is provided on the top of the housing (1), a vacuum tube placement plate (3) is provided inside the housing (1), and the vacuum tube placement plate (3) has a vacuum tube placement hole (31). The vacuum tube placement tray (3) is connected to the placement tray frame (4). The vacuum tube placement tray (3) is surrounded by a sealing barrier (5). The device housing (1) is equipped with a serum collection mechanism (6). The serum collection mechanism (6) is equipped with a serum extraction and transfer mechanism (7). The device housing (1) is equipped with a cleaning and disinfection mechanism (8).

2. The automatic animal serum separation and dispensing device according to claim 1, characterized in that, The serum collection device (6) includes a serum extraction needle (61) connected to a delivery line (62).

3. The automatic animal serum separation and dispensing device according to claim 2, characterized in that, The serum extraction and transfer mechanism (7) includes a serum extraction reversing frame (71), which is connected to a steering motor (73) via an extraction reversing shaft (72).

4. The automatic animal serum separation and dispensing device according to claim 3, characterized in that, The cleaning and disinfection mechanism (8) includes a rinsing and disinfection spray pipe (81), which is equipped with a rinsing and disinfection nozzle (82). The rinsing and disinfection spray pipe (81) is connected to a cleaning and disinfection solution injection pump (83) via a pipeline.

5. The automatic animal serum separation and dispensing device according to claim 4, characterized in that, A serum level monitoring probe (9) is provided on the side of the vacuum tube placement hole (31), and a serum biochemical index rapid detection plate (10) is provided on the side of the serum extraction needle tube (61).

6. The automatic animal serum separation and dispensing device according to claim 5, characterized in that, A control switch (11) is provided on the side of the outer shell (1) of the device, and a centrifugal force field dynamic balance turntable (12) is provided inside the outer shell (1).

7. The automatic animal serum separation and dispensing device according to claim 6, characterized in that, The centrifugal force field dynamic balance turntable (12) is provided with a multi-channel divergent partition plate (13) on the outside, and the upper part of the centrifugal force field dynamic balance turntable (12) is connected to an anti-vibration rotor raft (14).

8. The automatic animal serum separation and dispensing device according to claim 7, characterized in that, The device housing (1) has a heat dissipation grid (15) on the lower inner side, a storage battery (16) is installed inside the device housing (1), a caster wheel (17) is installed at the bottom of the device housing (1), and an adjustment support pad (18) is also installed at the bottom of the device housing (1).

9. The dispensing method of the automatic animal serum separation and dispensing device according to claim 8, characterized in that, Includes the following steps; Step 1: Preliminary preparation and debugging of the device: Adjust the outer shell (1) of the device to a horizontal and stable state by adjusting the support pad (18), close the locking structure of the universal wheel (17), press the control switch (11) to start the device, and power it by the storage battery (16). Place the vacuum tubes to be packaged into the vacuum tube placement holes (31) of the vacuum tube placement tray (3), and use the sealing barrier (5) to initially seal the area of ​​the vacuum tube placement tray (3) to prevent external contamination. Start the cleaning and disinfection mechanism (8): The cleaning and disinfection solution is delivered to the rinsing and disinfection spray nozzle (81) by the cleaning and disinfection solution filling pump (83). The serum extraction needle (61), delivery pipeline (62) and vacuum tube placement tray (3) are thoroughly rinsed and disinfected through the rinsing and disinfection nozzle (82). After completion, the waste liquid is discharged. Step 2: Serum extraction and transport preparation: The serum extraction and transfer mechanism (7) is started, and the steering motor (73) drives the serum extraction reversing frame (71) to rotate through the extraction reversing shaft (72), adjusting the serum extraction needle (61) to the sample position of the serum to be extracted and aligning it with the sample container; The serum collection mechanism (6) is started, the serum extraction needle (61) is inserted into the sample container, and the serum is extracted by negative pressure. The serum is transferred into the device through the delivery pipeline (62). During the transfer, the serum flows through the serum biochemical index rapid detection plate (10) to preliminarily detect the basic biochemical index of the serum and confirm that the serum meets the dispensing standard. Step 3: Centrifugation process: The extracted serum is transported to the area of ​​the centrifugal force field dynamic equilibrium turntable (12); The vibration acceleration of the centrifugal force field dynamic equilibrium turntable (12) satisfies: in: The maximum vibration acceleration of the centrifugal force field dynamic equilibrium turntable (12); This is the distance from the center of the turntable to the serum placement position; is the stiffness coefficient of the vibration-resistant rotor raft (14); This is the initial eccentricity of the turntable; The total mass of the turntable and serum; The natural vibration angular frequency of the vibration-resistant rotor raft (14); The multi-channel divergent separator (13) diverts serum to different centrifugation channels, and the anti-vibration rotor raft (14) buffers the vibration during centrifugation. The centrifugal force field dynamic balance turntable (12) is started, which drives the serum to rotate at high speed. Centrifugal force is used to separate the serum from blood cells and other impurities. During the separation process, the heat generated by centrifugation is dissipated through the heat dissipation grid (15) to maintain the stable internal temperature of the device. The dynamic balance correction formula for the vacuum tube placement tray (3) is: in: The unbalanced force that needs to be corrected in real time for the vacuum tube placement plate (3); For the first The total mass of the vacuum tube and the serum inside; The rotational angular velocity of the centrifugal force field dynamic equilibrium turntable (12); For the first The radial mounting radius of each vacuum tube on the vacuum tube placement plate (3); For the first The circumferential installation angle of each vacuum tube; The average circumferential installation angle for all vacuum tubes; The total number of vacuum tube placement holes (31); Step 4: Precise dispensing: After separation, the upper serum layer re-aggregates, and the serum extraction and transfer mechanism (7) readjusts the position of the serum extraction needle (61) and aligns it with the vacuum tube in the vacuum tube placement hole (31). The serum is injected into the vacuum tube through the delivery line (62) and the serum extraction needle (61), and the serum level monitoring probe (9) monitors the serum level in the vacuum tube in real time. The error correction formula for the serum level monitoring probe (9) is as follows: in: This is the correction value for liquid level detection error; The probe is used to detect the Poisson's ratio of the diaphragm; This represents the actual serum level. To detect the elastic modulus of the diaphragm; To detect the thickness of the membrane; The temperature influence coefficient of the probe material; When the liquid level reaches the preset value, stop adding liquid and complete the dispensing of a single vacuum tube; The formula for calculating the flow rate of the serum extraction needle (61) is as follows: in: Real-time extraction volume flow rate of serum extraction syringe (61); The inner diameter of the serum extraction needle (61); The outer diameter of the metal guidewire inside the syringe; The pressure difference between the two ends of the serum extraction needle (61); The dynamic viscosity of serum; The effective length of the serum extraction syringe (61); The friction coefficient of the pipeline (62); The equivalent length of the delivery pipeline (62); To measure the density of serum in real time; This is the reference density of serum under standard conditions; The placement tray (4) moves the vacuum tube placement tray (3) step by step, moving the next empty vacuum tube to the filling position. Repeat the above filling steps until all vacuum tubes are filled.

10. The dispensing method of the automatic animal serum separation and dispensing device according to claim 9, characterized in that, It also includes step five: finishing after dispensing: after all vacuum tubes are dispensed, the cleaning and disinfection mechanism (8) is started again to clean and disinfect the serum extraction needle (61) and delivery line (62) a second time to avoid cross-contamination; the control switch (11) is turned off, the battery (16) is disconnected from the power supply, the vacuum tubes that have been dispensed are taken out from the vacuum tube placement hole (31), the inside of the device is cleaned up, and the entire dispensing process is completed.

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