Composite bubble inhibiting method for sewage biological reaction tank and application thereof

Abstract

The application provides a composite foam inhibition method for a sewage biological reaction tank and application thereof; the composite foam inhibition method for the sewage biological reaction tank comprises the following steps: S1, providing a chemical defoaming agent and an additive; the additive comprises one or more of pig kidney acylase and vanillin; S2, adding the chemical defoaming agent and the additive into sewage, and aerating the sewage. The application can not only reduce the surface tension of foam liquid film, destroy the structure of the foam liquid film, realize rapid defoaming, but also inhibit excessive proliferation of filamentous bacteria, reduce foam generation from the source, can be directly applied to foam-prone units such as activated sludge biochemical tanks and secondary sedimentation tanks in sewage plants, is simple to operate, and has significant economic benefits and good application prospect.

Description

Technical Field

[0001] This invention belongs to the field of wastewater treatment, and particularly relates to a composite foam suppression method and its application in wastewater biological reactors. Background Technology

[0002] The activated sludge process is one of the most commonly used biological treatment technologies in wastewater treatment. It mainly utilizes microbial communities to degrade organic pollutants in wastewater, offering advantages such as high pollutant removal rates, strong adaptability, and high operational controllability. However, in actual operation, continuous aeration and the large-scale proliferation of filamentous bacteria in the reaction tank often lead to the formation of biological foam. The presence of biological foam easily increases the suspended solids in the effluent, further reducing effluent quality. Moreover, the high concentration of pathogenic bacteria in the foam carries antibiotic resistance genes that can easily spread via aerosols, posing a serious threat to public health and safety.

[0003] Chemical defoamers are a common method for controlling biological foam. They can rapidly disrupt foam stability, significantly reduce foam volume in a short time, and require small dosages with low operating costs. Depending on their chemical composition and mechanism of action, commonly used defoamers in wastewater treatment mainly include organosilicones, polyethers, mineral oils, alcohols, and fatty acid esters, which offer excellent defoaming effects. However, chemical defoamers primarily suppress foaming and cannot effectively address the underlying causes of foaming (such as the proliferation of filamentous bacteria). Therefore, they require continuous addition, resulting in high cumulative costs and potential residue risks.

[0004] Therefore, it is necessary to provide a composite defoaming method and its application for wastewater biological reactors to solve or at least alleviate the technical problem of how to significantly reduce the abundance of filamentous bacteria while defoaming. Summary of the Invention

[0005] The main objective of this invention is to provide a composite defoaming method and its application for wastewater biological reactors, aiming to solve or at least alleviate the technical problem of how to significantly reduce the abundance of filamentous bacteria while defoaming.

[0006] To achieve the above objectives, the present invention provides a composite foam suppression method for wastewater biological reactors, comprising the following steps: S1 provides chemical defoamers and additives; the additives include one or more of porcine kidney acylase and vanillin; S2, the chemical defoamer and the additive are added to the wastewater and the wastewater is aerated; the wastewater contains filamentous bacteria, and the concentration of the additive after addition is 20~100mg / L.

[0007] Furthermore, the dissolved oxygen in the wastewater is controlled at 0.5~2.0 mg / L.

[0008] Furthermore, the chemical defoamer includes one or more of fatty alcohol defoamers and organosilicon defoamers.

[0009] Furthermore, the chemical defoamer is the fatty alcohol defoamer; the volume percentage of the chemical defoamer and the wastewater is 0.2-1%.

[0010] Furthermore, the aeration period is no less than 3 days.

[0011] Furthermore, the additive is the porcine kidney acylase, and the concentration of the porcine kidney acylase after addition is 20~35 mg / L.

[0012] Furthermore, the additive is vanillin, and the concentration of vanillin after addition is 40~60 mg / L.

[0013] Furthermore, the wastewater originates from a sludge-sewage reaction tank; the wastewater reaction tank is an aeration tank with added activated sludge.

[0014] The present invention also provides the application of an additive in inhibiting the abundance of filamentous bacteria in sewage, said additive comprising one or more of porcine kidney acylase and vanillin.

[0015] The present invention also provides an application of the composite foam suppression method as described above in wastewater treatment.

[0016] Compared with the prior art, the present invention has at least the following advantages: This invention can significantly reduce the abundance of filamentous bacteria while defoaming. It not only has excellent defoaming effects but also significantly inhibits the proliferation of filamentous bacteria in biological foam, achieving long-term control of biological foam. This dual-action mechanism gives the composite approach of this invention significant technical advantages and broad application prospects in the field of wastewater treatment. Specifically, this application introduces porcine kidney acylase and / or vanillin as additives on the basis of chemical defoamers. Through the synergistic effect of porcine kidney acylase, vanillin, and chemical defoamers, not only can biological foam be controlled, but the abundance of filamentous bacteria can also be significantly reduced, optimally to 0.16%. Therefore, this invention can produce both immediate defoaming and long-term regulation, with advantages such as long-lasting action, low dosage, and eco-friendliness. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without creative effort.

[0018] Figure 1 This refers to the foaming tendency assessment results in Example 1 of the present invention; Figure 2 The results of the microbial community composition assessment in Example 2 of this invention are shown; where (a) represents the top ten genera in terms of relative abundance, and (b) represents filamentous fungi. Skermania The relative abundance; Figure 3 This invention analyzes Example 3, comparing the bacterial community composition evaluation results after treatment with 25 mg / L porcine kidney acylase or 50 mg / L vanillin; where (a) represents the top ten genera in relative abundance, and (b) represents filamentous fungi. Skermania The relative abundance; Figure 4 The above are the foaming tendency and filamentous bacteria relative abundance assessment results in Example 1 of the present invention, wherein (a) is the foaming tendency assessment result, and (b) is the filamentous bacteria... Skermania The relative abundance of.

[0019] The realization of the objective, functional characteristics and advantages of the present invention will be further explained in conjunction with the embodiments and with reference to the accompanying drawings. Detailed Implementation

[0020] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of them. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0021] Furthermore, the technical solutions of the various embodiments of the present invention can be combined with each other, but only if they are based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such combination of technical solutions does not exist and is not within the scope of protection claimed by the present invention.

[0022] When numerical ranges are given in the embodiments, it should be understood that, unless otherwise stated in the present invention, both endpoints of each numerical range and any value between the two endpoints may be selected. Unless otherwise defined, all technical and scientific terms used in this invention, as well as the prior art known to those skilled in the art and the description of this invention, may be implemented using any prior art methods, devices, and materials similar to or equivalent to those described, used, or made of materials in the embodiments of this invention.

[0023] This invention provides a composite foam suppression method for wastewater biological reactors, comprising the following steps: S1 provides chemical defoamers and additives.

[0024] In this invention, the additive includes or may be one or more of porcine kidney acylase and vanillin; in this case, the porcine kidney acylase is derived from Sigma-Aldrich A8376-10g, and the vanillin is derived from Sigma-Aldrich V1104-100g.

[0025] In this invention, the chemical defoamer includes or is one or more of fatty alcohol defoamers and organosilicon defoamers; further, the chemical defoamer is the fatty alcohol defoamer.

[0026] In this invention, the silicone defoamer used is milky white in appearance, with a viscosity of 2000 mPa·s at 25°C and a pH of 7.0. In this case, the silicone defoamer is sourced from Foshan Jingqi Chemical Technology Co., Ltd.'s 610 defoamer, and its active ingredient is 4.5% silicone oil.

[0027] In this invention, the fatty alcohol defoamer is pale yellow in appearance, has a viscosity of 260 mPa·s at 25°C, and a pH of 7.0. In this case, the fatty alcohol defoamer is sourced from Dongguan Guozhong New Materials Research Institute Co., Ltd., specifically DU-1383, and its active ingredient is 99% fatty alcohol.

[0028] In this invention, when the organosilicone defoamer is added to the wastewater alone and then aerated, and the dosage of the organosilicone defoamer is 0.25% (V / V), the foaming tendency of the wastewater after aeration for 4 days under dissolved oxygen conditions of 1 mg / L is 0.20~0.22 (mL·foam) / (mL·air·min), filamentous bacteria... Skermania The relative abundance was 0.88–0.90%; when the addition amount was 0.5% (V / V), the foaming tendency of the wastewater after aeration for 4 days under dissolved oxygen conditions of 1 mg / L was 0–0.02 (mL·foam) / (mL·air·min), filamentous bacteria Skermania The relative abundance was 2.24~2.26%; when the addition amount was 1% (V / V), the foaming tendency of the wastewater after aeration for 4 days under dissolved oxygen conditions of 1 mg / L was 0 (mL·foam) / (mL·air·min), filamentous bacteria Skermania The relative abundance is 0.72–0.74%.

[0029] In this invention, when the fatty alcohol defoamer is added to the wastewater alone and then aerated, and the amount of the fatty alcohol defoamer added is 0.25% (V / V), the foaming tendency of the wastewater after aeration for 4 days under dissolved oxygen conditions of 1 mg / L is 0 (mL·foam) / (mL·air·min), and filamentous bacteria... SkermaniaThe relative abundance was 1.27~1.29%; when the addition amount was 0.5% (V / V), the foaming tendency of the wastewater after aeration for 4 days under dissolved oxygen conditions of 1 mg / L was 0 (mL·foam) / (mL·air·min), filamentous bacteria Skermania The relative abundance was 2.39~2.41%; when the addition amount was 1% (V / V), the foaming tendency of the wastewater after aeration for 4 days under dissolved oxygen conditions of 1 mg / L was 0 (mL·foam) / (mL·air·min), filamentous bacteria Skermania The relative abundance is 0.96–0.98%.

[0030] S2, the chemical defoamer and the additive are added to the wastewater and the wastewater is aerated; the aeration is continuous aeration.

[0031] The wastewater of this invention contains filamentous bacteria; the wastewater originates from a wastewater biological reactor; the wastewater biological reactor is an aeration tank with added activated sludge, specifically an aerobic tank in a wastewater treatment plant. In some embodiments of this invention, the wastewater is taken from the foam layer of the wastewater reactor.

[0032] As a further explanation of the wastewater without the addition of any additional agents in this invention, the foaming tendency of the wastewater after aeration for 4 days under dissolved oxygen conditions of 1 mg / L is 0.22~0.24 (mL·foam) / (mL·air·min); the filamentous bacteria of the wastewater after aeration for 4 days under dissolved oxygen conditions of 1 mg / L... Skermania The relative abundance was 1.80~1.85%. Specifically, the foaming tendency of the wastewater after aeration for 4 days under dissolved oxygen conditions of 1 mg / L was 0.23 (mL·foam) / (mL·air·min); the filamentous bacteria of the wastewater after aeration for 4 days under dissolved oxygen conditions of 1 mg / L... Skermania The relative abundance was 1.82%.

[0033] In this invention, the concentration of the additive after addition is 20-100 mg / L; as one embodiment, the additive is porcine kidney acylase, and the concentration of the porcine kidney acylase after addition is 20-35 mg / L, more specifically 20-30 mg / L, more specifically 24-26 mg / L; as another embodiment, the additive is vanillin, and the concentration of the vanillin after addition is 40-60 mg / L, more specifically 45-55 mg / L, more specifically 49-51 mg / L.

[0034] In this invention, when the chemical defoamer is the fatty alcohol defoamer, the volume percentage of the chemical defoamer and the wastewater is 0.2-1%; further, the volume percentage of the chemical defoamer and the wastewater is 0.2-0.3%, and more specifically 0.24-0.26%.

[0035] In this invention, during the aeration of the wastewater after adding the chemical defoamer and the additive, the dissolved oxygen in the wastewater is controlled at 0.5~2.0 mg / L, more specifically 0.9~1.1 mg / L. After adding the chemical defoamer and the additive, the aeration time is not less than 3 days, more specifically 3~5 days, more specifically 4~5 days. Specifically, after 4 days of aeration, the foaming tendency is 0 (mL·foam) / (mL·air·min); filamentous bacteria... Skermania The relative abundance was 0.15–0.65%, further ranging from 0.16–0.63% to 0.15–0.2% to 0.15–0.17% to 0.62–0.65% to 0.62–0.64%.

[0036] This invention also provides the application of an additive in suppressing the abundance of filamentous bacteria in wastewater, said additive comprising one or more of porcine kidney acylase and vanillin. This invention significantly reduces the abundance of filamentous bacteria by adding said porcine kidney acylase and / or said vanillin to wastewater.

[0037] In the application process, the additive is added to the wastewater, and the wastewater is aerated; the aeration is continuous; the aeration duration is not less than 3 days, more specifically 3-5 days, and more specifically 4-5 days. During the aeration of the wastewater, the dissolved oxygen in the wastewater is controlled at 0.5-2.0 mg / L, and more specifically 0.9-1.1 mg / L.

[0038] In the aforementioned applications, the concentration of the additive after addition is 20-100 mg / L; as one embodiment, the additive is porcine kidney acylase, and the concentration of the porcine kidney acylase after addition is 20-35 mg / L, further 20-30 mg / L, further 24-26 mg / L; as another embodiment, the additive is vanillin, and the concentration of the vanillin after addition is 40-60 mg / L, further 45-55 mg / L, further 49-51 mg / L.

[0039] The present invention also provides an application of the composite foam suppression method as described above in wastewater treatment.

[0040] This invention combines chemical defoamers with specific additives to create a novel biological foam inhibitor. It not only reduces the surface tension of the foam film and disrupts its structure for rapid defoaming, but also inhibits the excessive proliferation of filamentous bacteria, reducing foam generation at the source. It is an environmentally friendly, highly efficient, and stable composite foam inhibitor that can be directly applied to foam-prone units such as activated sludge biological treatment tanks and secondary sedimentation tanks in wastewater treatment plants. It is easy to operate and has significant economic benefits and promising application prospects.

[0041] In this invention, the foaming tendency test method is the aeration method: Pour 200 mL of the sample into the testing device, aerate from the bottom at an air flow rate of 3 L / min for 15 s, and record the foam volume. The foaming potential FT = V. f / Q air V f Q represents the volume of foam produced after aeration (mL). air The gas flow rate is expressed in mL / min.

[0042] In this invention, the method for testing the bacterial community composition is the 16S rRNA gene high-throughput sequencing method: DNA was extracted from 0.5 mL foam samples using the FastDNA SPIN kit. DNA quality was assessed using NanoDrop and agarose gel electrophoresis. The V3-V4 region of the bacterial 16S rRNA gene was amplified using primers 341F (5'-CCTAYGGGRBGCASCAG-3') and 806R (5'-GGACTACNNGGGTATCTAAT-3'). The PCR products were purified and used to construct an Illumina sequencing library. Data quality control and OTU clustering (97% similarity) were performed using Qiime and USEARCH. Species annotation was performed using the Silva database (V138) via RDPclassifier (confidence threshold 0.7).

[0043] The following are specific examples of the present invention: Analysis Example 1 Biological foam was collected from the aerobic tank of the activated sludge process at a municipal wastewater treatment plant in Kaifu District, Changsha City. A columnar plastic sampler was used for collection and fixed at a specific depth (±0.1m from the water surface) with a rope. The container opening was slowly brought close to the foam layer, allowing the foam to flow naturally into the container. To ensure that the collected foam was representative, multiple points were collected at different locations. During the collection process, the container was kept away from contacting the activated sludge below the water surface to avoid contamination of impurities.

[0044] This analytical example was conducted using the following method: Organosilicon group: Add organosilicon defoamer to 400mL of biofoam at volume ratios of 0.25%, 0.5%, and 1%, respectively, and stir thoroughly. Then, continue aeration, with dissolved oxygen controlled at 1.0 mg / L during the aeration process, and the aeration air source is air.

[0045] Fatty alcohol group: Fatty alcohol defoamer was added to 400mL of biological foam at volume ratios of 0.25%, 0.5%, and 1%, respectively, and stirred thoroughly. Then, continuous aeration was carried out, with dissolved oxygen controlled at 1.0mg / L during the aeration process, and air was used as the aeration source.

[0046] Control group (original foam): The biological foam without any additional exogenous substances was continuously aerated. During the aeration process, the dissolved oxygen was controlled at 1.0 mg / L, and the aeration source was air.

[0047] In this analysis, the foaming tendency of each group was measured 4 days after aeration.

[0048] See Figure 1 As shown, silicone defoamers and fatty alcohol defoamers have an inhibitory effect on the foaming of biological foam; when the amount of silicone defoamer added is 1% (V / V) and the amount of fatty alcohol defoamer added is 0.25% (V / V), no foaming occurs at all.

[0049] Table 1 shows the experimental results of foaming tendency under different volume ratios of silicone defoamer and fatty alcohol defoamer in this analysis example.

[0050] Table 1. Analysis of Foaming Tendency Analysis Example 2 The same experiments were conducted as in Example 1, and 16S rRNA high-throughput sequencing technology was used to analyze the microbial community of samples collected 4 days after aeration treatment, determining the genus-level microbial community composition and relative abundance, as well as specific filamentous bacteria. Skermania The relative abundance of.

[0051] See Figure 2 As shown in section (a), in this analytical example, bacteria with both denitrification and phosphorus removal functions... unclassified_ f__Comamonadaceae Heterotrophic denitrifying bacteria Acidovarax , Simplicispira All of them have a certain abundance.

[0052] See Figure 2 As shown in section (b) of this analysis, in the treatment groups with added 0.25% and 1% (v / v) silicone defoamer, after 4 days of aeration, filamentous bacteria... SkermaniaThe relative abundances were 0.89% and 0.73%, respectively; in the treatment groups with 0.25% and 1% (v / v) fatty alcohol defoamer, the filamentous bacteria... Skermania The relative abundances were 1.28% and 0.97%, respectively.

[0053] Table 2 shows the filamentous bacteria under different volume ratios of organosilicon defoamer and fatty alcohol defoamer in this analysis example. Skermania Experimental results on relative abundance.

[0054] Table 2. Relative abundance analysis of filamentous fungi Analysis Example 3 In this analysis, the source of the biological foam used in the experiment is the same as in Analysis Example 1.

[0055] This analytical example was conducted using the following method: Porcine kidney acylase group: Porcine kidney acylase was added to 400 mL of biological foam at dosages of 25 mg / L, 50 mg / L, and 100 mg / L, respectively, and stirred thoroughly. Then, continuous aeration was carried out, with dissolved oxygen controlled at 1.0 mg / L during the aeration process, and air was used as the aeration source.

[0056] Vanillin group: Vanillin was added to 400mL of biological foam at dosages of 25mg / L, 50mg / L and 100mg / L respectively, and stirred thoroughly. Then, aeration was carried out continuously. During the aeration process, dissolved oxygen was controlled at 1.0mg / L, and the aeration gas source was air.

[0057] Control group (original foam): The biological foam without any additional exogenous substances was continuously aerated. During the aeration process, the dissolved oxygen was controlled at 1.0 mg / L, and the aeration source was air.

[0058] 16S rRNA high-throughput sequencing technology was used to analyze the microbial community of samples collected 4 days after aeration treatment, determining the genus-level microbial community composition and relative abundance, as well as specific filamentous bacteria. Skermania The relative abundance of.

[0059] like Figure 3 As shown in section (a) of this analysis, in the treatment group with 25 mg / L porcine kidney acylase and the treatment group with 50 mg / L vanillin, unclassified_f__Comamonadaceae, Acidovarax , Simplicispira All functional bacteria were present in a certain abundance.

[0060] like Figure 3 As shown in section (b), in the treatment group with added 25 mg / L porcine kidney acylase, after 4 days of aeration... SkermaniaThe relative abundance was 0.27%; in the treatment group with added 50 mg / L vanillin, after 4 days of aeration... Skermania The relative abundance was 0.73%.

[0061] Table 3 shows the filamentous fungi under different dosages of porcine kidney acylase and vanillin in this analysis. Skermania Experimental results on relative abundance.

[0062] Table 3. Relative abundance analysis of filamentous fungi Example 1 In this embodiment, the source of the biological foam used in the experiment is the same as in Analysis Example 1.

[0063] This embodiment of the experiment was conducted using the following method: Experimental group: Porcine kidney acylase and fatty alcohol defoamer were added to 400 mL of biological foam. The dosage of porcine kidney acylase was 25 mg / L, and the volume percentage of fatty alcohol defoamer and biological foam was 0.25% (V / V). The mixture was stirred thoroughly. Then, aeration was carried out continuously. During the aeration process, the dissolved oxygen was controlled at 1.0 mg / L, and the aeration air source was air.

[0064] Control group (original foam): The biological foam without any additional exogenous substances was continuously aerated. During the aeration process, the dissolved oxygen was controlled at 1.0 mg / L, and the aeration source was air.

[0065] In this embodiment, samples collected after 4 days of aeration treatment were analyzed for foaming tendency and specific filamentous bacteria. Skermania The relative abundance of [the species] was determined.

[0066] See Figure 4 As shown in parts (a) and (b), the combination of 25 mg / L porcine kidney acylase and 0.25% fatty alcohol defoamer exhibited good antifoaming performance, and the relative abundance of filamentous bacteria also decreased significantly. This combination method can not only quickly eliminate foam by reducing surface tension at the chemical level, but also effectively regulate the microbial community structure and inhibit the abundance of filamentous bacteria at the biological level, realizing a dual synergistic mechanism of "chemical defoaming-biological regulation".

[0067] In the control group of this embodiment, the foaming tendency after 4 days of aeration was 0.23 (mL·foam) / (mL·air·min), filamentous fungi Skermania The relative abundance was 1.82%.

[0068] In the experimental group of this embodiment, the foaming tendency after 4 days of aeration was 0 (mL·foam) / (mL·air·min), and the filamentous fungi... SkermaniaThe relative abundance was 0.16%.

[0069] Comparative Example 1 In this comparative example, the source of the biological foam used in the experiment is the same as in Analytical Example 1.

[0070] This comparative example was conducted using the following method: Experimental group: Porcine kidney acylase and fatty alcohol defoamer were added to 400 mL of biological foam. The dosage of porcine kidney acylase was 100 mg / L, and the volume percentage of fatty alcohol defoamer and biological foam was 0.25% (V / V). The mixture was stirred thoroughly. Then, aeration was carried out continuously. During the aeration process, the dissolved oxygen was controlled at 1.0 mg / L, and the aeration air source was air.

[0071] Control group (original foam): The biological foam without any additional exogenous substances was continuously aerated. During the aeration process, the dissolved oxygen was controlled at 1.0 mg / L, and the aeration source was air.

[0072] In this comparative example, samples collected 4 days after aeration treatment were analyzed for foaming tendency and specific filamentous bacteria. Skermania The relative abundance of [the species] was determined.

[0073] In the control group of this comparative example, the foaming tendency after 4 days of aeration was 0.23 (mL·foam) / (mL·air·min), filamentous fungi Skermania The relative abundance was 1.82%.

[0074] In this comparative experimental group, the foaming tendency after 4 days of aeration was 0 (mL·foam) / (mL·air·min), and the filamentous bacteria... Skermania The relative abundance was 1.72%.

[0075] Example 2 In this embodiment, the source of the biological foam used in the experiment is the same as in Analysis Example 1.

[0076] This embodiment of the experiment was conducted using the following method: Experimental group: Vanillin and fatty alcohol defoamer were added to 400 mL of biological foam. The amount of vanillin added was 50 mg / L, and the volume percentage of fatty alcohol defoamer and biological foam was 0.25% (V / V). The mixture was stirred thoroughly. Then, aeration was carried out continuously. During the aeration process, the dissolved oxygen was controlled at 1.0 mg / L, and the aeration air source was air.

[0077] Control group (original foam): The biological foam without any additional exogenous substances was continuously aerated. During the aeration process, the dissolved oxygen was controlled at 1.0 mg / L, and the aeration source was air.

[0078] In this embodiment, samples collected after 4 days of aeration treatment were analyzed for foaming tendency and specific filamentous bacteria. Skermania The relative abundance of [the species] was determined.

[0079] In the control group of this embodiment, the foaming tendency after 4 days of aeration was 0.23 (mL·foam) / (mL·air·min), filamentous fungi Skermania The relative abundance was 1.82%.

[0080] In the experimental group of this embodiment, the foaming tendency after 4 days of aeration was 0 (mL·foam) / (mL·air·min), and the filamentous fungi... Skermania The relative abundance was 0.63%.

[0081] Comparative Example 2 In this comparative example, the source of the biological foam used in the experiment is the same as in Analytical Example 1.

[0082] This comparative example was conducted using the following method: Experimental group: Vanillin and fatty alcohol defoamer were added to 400 mL of biological foam. The amount of vanillin added was 100 mg / L, and the volume percentage of fatty alcohol defoamer and biological foam was 0.25% (V / V). The mixture was stirred thoroughly. Then, aeration was carried out continuously. During the aeration process, the dissolved oxygen was controlled at 1.0 mg / L, and the aeration air source was air.

[0083] Control group (original foam): The biological foam without any additional exogenous substances was continuously aerated. During the aeration process, the dissolved oxygen was controlled at 1.0 mg / L, and the aeration source was air.

[0084] In this comparative example, samples collected 4 days after aeration treatment were analyzed for foaming tendency and specific filamentous bacteria. Skermania The relative abundance of [the species] was determined.

[0085] In the control group of this comparative example, the foaming tendency after 4 days of aeration was 0.23 (mL·foam) / (mL·air·min), filamentous fungi Skermania The relative abundance was 1.82%.

[0086] In this comparative experimental group, the foaming tendency after 4 days of aeration was 0 (mL·foam) / (mL·air·min), and the filamentous bacteria... Skermania The relative abundance was 1.35%.

[0087] The above technical solutions of the present invention are merely preferred embodiments of the present invention and do not limit the patent scope of the present invention. All equivalent structural transformations made under the technical concept of the present invention using the contents of the present invention specification and drawings, or direct / indirect applications in other related technical fields, are included in the patent protection scope of the present invention.

Claims

1. A composite foam suppression method for a wastewater biological reactor, characterized in that, Including the following steps: S1 provides chemical defoamers and additives; the additives include one or more of porcine kidney acylase and vanillin; S2, the chemical defoamer and the additive are added to the wastewater and the wastewater is aerated; the wastewater contains filamentous bacteria, and the concentration of the additive after addition is 20~100mg / L.

2. The composite foam suppression method for wastewater biological reactors according to claim 1, characterized in that, The dissolved oxygen in the wastewater is controlled at 0.5~2.0 mg / L.

3. The composite foam suppression method for wastewater biological reactors according to claim 1, characterized in that, The chemical defoamer includes one or more of fatty alcohol defoamers and organosilicon defoamers.

4. The composite foam suppression method for a wastewater biological reactor according to claim 3, characterized in that, The chemical defoamer is the fatty alcohol defoamer; the volume percentage of the chemical defoamer and the wastewater is 0.2-1%.

5. The composite foam suppression method for a wastewater biological reactor according to claim 1, characterized in that, The aeration period shall be no less than 3 days.

6. The composite foam suppression method for a wastewater biological reactor according to claim 1, characterized in that, The additive is the porcine kidney acylase, and the concentration of the porcine kidney acylase after addition is 20~35 mg / L.

7. The composite foam suppression method for a wastewater biological reactor according to claim 1, characterized in that, The additive is vanillin, and the concentration of vanillin after addition is 40~60 mg / L.

8. The composite foam suppression method for wastewater biological reactors according to claim 1, characterized in that, The wastewater originates from a sludge-sewage reaction tank; the wastewater reaction tank is an aeration tank into which activated sludge is added.

9. The application of an additive in inhibiting the abundance of filamentous bacteria in sewage, characterized in that, The additives include one or more of porcine kidney acylase and vanillin.

10. The application of the composite foam suppression method as described in any one of claims 1-8 in wastewater treatment.

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