A functional organic fertilizer for reducing greenhouse gas emission and improving quality of tomatoes, and a preparation method and application thereof
The functional organic fertilizer, made by fermenting algae-bacteria symbiosis with modified olivine powder and chicken manure, solves the problems of greenhouse gas emissions and fruit quality improvement in tomato cultivation caused by existing organic fertilizers, and achieves low carbon emissions and quality improvement in the tomato cultivation process.
Patent Information
- Application Number
- CN202610325441.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-03-17
- Publication Date
- 2026-06-09
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Abstract
Description
Technical Field
[0001] This invention relates to the field of bio-fertilizers, and in particular to a functional organic fertilizer that reduces greenhouse gas emissions from tomatoes and improves tomato quality, as well as its preparation method and application. Background Technology
[0002] With the intensification of climate change and the increasing urgency of sustainable agricultural development, the synergistic improvement of greenhouse gas emissions and crop quality in greenhouse agriculture has become a key area of research in modern agriculture. Tomatoes, as one of the world's highest-yielding greenhouse vegetables, generate greenhouse gases (including N2O, CH4, and CO2) primarily from soil nitrification-denitrification and organic matter decomposition caused by excessive application of chemical fertilizers. Statistics show that the greenhouse gas emission intensity per unit area of greenhouse vegetable production systems can be 3-5 times that of field crops, with nitrogen fertilizer contributing over 60%. Simultaneously, long-term application of chemical fertilizers leads to soil acidification and microbial community imbalance, significantly reducing key quality indicators such as vitamin C and soluble sugars in tomato fruits, directly affecting their nutritional and commercial value. While currently available organic fertilizers can partially replace chemical fertilizers, their practical application generally suffers from drawbacks such as uncontrollable nutrient release, difficulty in maintaining microbial activity, and insufficient heavy metal adsorption capacity, making it difficult to achieve a synergistic effect of greenhouse gas emission reduction and quality improvement.
[0003] Therefore, there is an urgent need to provide a new type of organic fertilizer that combines the advantages of environmental protection and sustainable utilization, achieving an organic unity of environmental and economic benefits, and providing an innovative solution for the green transformation of facility agriculture. Summary of the Invention
[0004] The purpose of this invention is to provide a functional organic fertilizer that reduces greenhouse gas emissions from tomatoes and improves tomato quality, along with its preparation method and application, to address the problems existing in the prior art. This invention cultivates *Chlorella* and *Streptomyces flavus* to create an algae-fungus symbiosis, which is then co-fermented with chicken manure and calcined modified olivine powder to produce the functional organic fertilizer. The functional organic fertilizer provided by this invention can significantly reduce the emission rate of greenhouse gases such as carbon dioxide, methane, and nitrous oxide during tomato cultivation, increase soil carbon sequestration, and effectively improve tomato fruit quality.
[0005] To achieve the above objectives, the present invention provides the following solution: This invention provides a method for preparing functional organic fertilizer, comprising the following steps: The functional organic fertilizer is obtained by mixing algae-bacteria symbiosis, modified olivine powder and chicken manure and then fermenting the mixture.
[0006] Furthermore, the mass ratio of the algae-bacterial symbiosis, the modified olivine powder, and the chicken manure is 1:(1-2):(2-4).
[0007] Furthermore, the algae-bacterial symbiotic is obtained by inoculating Chlorella and Streptomyces flavus into a culture medium and then co-amplifying and culturing them.
[0008] Furthermore, the amplification culture is subjected to a light exposure time of no less than 12 h / d, a light intensity of 5000-5400 Lux, a temperature of 26℃, and a duration of 12 days.
[0009] Furthermore, the modified olivine powder is obtained by pulverizing, drying, and calcining olivine powder.
[0010] Furthermore, the drying temperature does not exceed 40°C, and the drying time is 24 hours; The calcination includes calcination at 200℃ for 2 hours and calcination at 450℃ for 2 hours.
[0011] Furthermore, the fermentation temperature is 25-35℃, and the time is 10-15 days.
[0012] The present invention also provides a functional organic fertilizer obtained by the above preparation method.
[0013] The present invention also provides an application of the above-mentioned functional organic fertilizer in mitigating greenhouse gas emissions from tomatoes.
[0014] The present invention also provides an application of the above-mentioned functional organic fertilizer in improving the quality of tomato fruits and / or leaves.
[0015] The present invention discloses the following technical effects: This invention relates to a functional organic fertilizer made from algae-bacteria symbiosis, modified olivine powder, and chicken manure. It can increase soil carbon sequestration, reduce carbon dioxide emissions, and, together with symbiotic nitrogen-fixing bacteria in the soil, promote nitrogen cycling, enhance soil nitrogen fixation capacity, and reduce nitrous oxide emissions. Furthermore, this functional organic fertilizer is environmentally friendly and sustainable, aligning with modern agricultural development trends. It is of great significance for further promoting the development and application of organic fertilizers and provides a new direction for low-carbon agricultural production.
[0016] The chicken manure in this functional organic fertilizer replenishes soil organic matter, provides a slow-release organic carbon source, and offers nutritional support for the growth of the algae-bacteria symbiotic organism. The *Chlorella vulgaris* and *Streptomyces flavus* within the algae-bacteria symbiotic organism secrete bioactive substances through synergistic action, significantly improving nitrogen and phosphorus utilization efficiency while also enhancing the soil's microecological environment. Furthermore, it activates minerals such as magnesium, calcium, iron, potassium, and sodium in the olivine powder. The modified olivine powder provides stable binding sites for organic matter in the soil, inhibiting microbial carbon utilization and achieving soil carbon fixation while reducing carbon emissions. The three components work synergistically to form a triple functional network of "microbial regulation - nutrient slow release - carbon sequestration." Detailed Implementation
[0017] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.
[0018] It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the invention. Furthermore, with respect to numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Any stated value or intermediate value within a stated range, as well as each smaller range between any other stated value or intermediate value within said range, is also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.
[0019] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.
[0020] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be apparent to those skilled in the art. This specification and embodiments are merely exemplary.
[0021] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.
[0022] The present invention uses Chlorella ( Chlorella sp. FACHB-28 was purchased from the Freshwater Algae Culture Bank of the Chinese Academy of Sciences; Streptomyces flavus ( Streptomyces microflavus Purchased from the China General Microbiological Culture Collection Center, CGMCC 4.1976.
[0023] The Spirulina used in this invention ( Spirulina The sp. was purchased from the Freshwater Algae Culture Bank of the Chinese Academy of Sciences, with the number FACHB-971.
[0024] The natural olivine powder used in this invention was purchased from ordinary commercial sources in Jiangxi Province, and its main component is magnesium iron silicate.
[0025] The culture medium (named Symbiont Amplification Medium) used in this invention for amplifying and culturing algae-bacteria symbionts comprises the following components: 1.5 g / L sodium nitrate, 40 mg / L dipotassium hydrogen phosphate trihydrate, 80 mg / L magnesium sulfate heptahydrate, 36 mg / L calcium chloride dihydrate, 20 mg / L sodium carbonate, 1 mg / L disodium EDTA, 0.72 mg / L ferric chloride hexahydrate, 6 mg / L citric acid, 6 mg / L ferric ammonium citrate, 3 mg / L boric acid, 2 mg / L manganese chloride tetrahydrate, 0.2 mg / L zinc sulfate heptahydrate, 0.08 mg / L copper sulfate pentahydrate, and 0.05 mg / L cobalt nitrate hexahydrate.
[0026] The chicken manure used in this invention is purchased from ordinary commercial sources.
[0027] Example 1 A functional organic fertilizer, by weight, consists of: 1 part algae-bacteria symbiosis, 1 part modified olivine powder and 2 parts chicken manure.
[0028] 1. Preparation method of algae-bacteria symbiont 150 mL of symbiotic amplification culture medium was transferred to a 250 mL Erlenmeyer flask and sterilized at 120°C for 2 hours. After cooling, the pH was adjusted to 6.5, and then 60 mL of the medium was inoculated at a density of 1×10⁻⁶ cells / mL in an aseptic environment. 6 Chlorella algae solution with a density of 2 × 10⁶ cells / mL and 20 mL of Chlorella algae solution with a density of 2 × 10⁶ cells / mL 8 The culture solution of *Streptomyces flavus* was inoculated at a concentration of 1 / mL. After inoculation, algae-bacterium amplification culture was carried out. During the algae-bacterium amplification culture, a light duration of 12 hours per day and a light intensity of 5000 Lux were maintained. The flasks were shaken three times a day (morning, noon, and evening) to provide dissolved oxygen. The culture temperature was 26℃, and the culture was carried out for 12 days to obtain the algae-bacterium symbiosis.
[0029] 2. Preparation method of modified olivine powder Natural olivine powder was crushed and ground, then passed through a 60-mesh nylon sieve. After washing away surface impurities with ultrapure water, it was dried in an oven at 40°C for 24 hours. Then, it was calcined at high temperature in two stages: first at 200°C for 2 hours, and then at 450°C for 2 hours, to obtain modified olivine powder.
[0030] 3. Preparation methods of functional organic fertilizers By weight, 1 part algae-bacterial symbiosis, 1 part modified olivine powder and 2 parts chicken manure are mixed to obtain a mixed system. The initial moisture content of the mixed system is adjusted to 50%-55%, and fermented at 25℃ for 10 days. From the 5th to the 10th day after the start of fermentation, the pile is turned over every two days to obtain functional organic fertilizer.
[0031] Example 2 A functional organic fertilizer, by weight, consists of: 1 part algae-bacteria symbiosis, 2 parts modified olivine powder and 2 parts chicken manure.
[0032] 1. Preparation method of algae-bacteria symbiont Take 150 mL of symbiotic culture medium and transfer it to a 250 mL Erlenmeyer flask. Sterilize the flask at 120°C for 2 hours. After cooling, adjust the pH to 7.0, and then inoculate 60 mL of the medium at a density of 1×10⁻⁶ cells / mL in a sterile environment. 6 Chlorella algae solution with a density of 2 × 10⁶ cells / mL and 20 mL of Chlorella algae solution with a density of 2 × 10⁶ cells / mL 8 The culture solution of *Streptomyces flavus* was inoculated at a concentration of 1 / mL. After inoculation, algae-bacterium amplification culture was carried out. During the algae-bacterium amplification culture, a light duration of 12 hours per day and a light intensity of 5200 Lux were maintained. The flasks were shaken three times a day (morning, noon, and evening) to provide dissolved oxygen. The culture was carried out for 12 days at a temperature of 26℃ to obtain the algae-bacterium symbiosis.
[0033] 2. The preparation method of the modified olivine powder is the same as in Example 1.
[0034] 3. Preparation methods of functional organic fertilizers By weight, 1 part algae-bacterial symbiosis, 2 parts modified olivine powder and 2 parts chicken manure are mixed to obtain a mixed system. The initial moisture content of the mixed system is adjusted to 50%-55%, and fermented at 25℃ for 15 days. The compost is turned over every two days from the 5th to the 15th day after the start of fermentation to obtain functional organic fertilizer.
[0035] Example 3 A functional organic fertilizer, by weight, consists of: 1 part algae-bacteria symbiosis, 2 parts modified olivine powder and 4 parts chicken manure.
[0036] 1. Preparation method of algae-bacteria symbiont Take 150 mL of algae-bacterial symbiotic culture medium and transfer it to a 250 mL Erlenmeyer flask. Sterilize the medium at 120°C for 2 hours. After cooling, adjust the pH to 7.5, and then inoculate 60 mL of the medium at a density of 1×10⁻⁶ cells / mL in a sterile environment. 6 Chlorella algae solution with a density of 2 × 10⁶ cells / mL and 20 mL of Chlorella algae solution with a density of 2 × 10⁶ cells / mL 8 The culture solution of *Streptomyces flavus* was inoculated at a concentration of 100 cells / mL. After inoculation, algae-bacterium amplification culture was carried out. During the algae-bacterium amplification culture, a light duration of 12 hours per day and a light intensity of 5400 Lux were maintained. The flasks were shaken three times a day (morning, noon, and evening) to provide dissolved oxygen. The culture was carried out for 12 days at a temperature of 26℃ to obtain the algae-bacterium symbiosis.
[0037] 2. The preparation method of the modified olivine powder is the same as in Example 1.
[0038] 3. Preparation methods of functional organic fertilizers By weight, 1 part algae-bacterial symbiosis, 2 parts modified olivine powder and 4 parts chicken manure are mixed to obtain a mixed system. The initial moisture content of the mixed system is adjusted to 50%-55%, and fermented at 35℃ for 10 days. The compost is turned over every two days from the 5th to the 10th day after the start of fermentation to obtain functional organic fertilizer.
[0039] Comparative Example 1 A functional organic fertilizer, the only difference in composition and preparation method from Example 2 is that when preparing the algae-bacterial symbiosis, the inoculation of 20 mL of Streptomyces flavus bacterial solution is replaced with the inoculation of 10 mL of Streptomyces flavus bacterial solution.
[0040] Comparative Example 2 A functional organic fertilizer, the only difference in composition and preparation method from Example 2 is that, in preparing the algae-bacteria symbiosis, the Chlorella liquid is replaced with an equal amount of Spirulina liquid, and the density of Spirulina is 1×10⁻⁶. 6 per mL.
[0041] Comparative Example 3 A functional organic fertilizer, the only difference in composition and preparation method from Example 2 is that the modified olivine powder is replaced with olivine powder that has only been washed.
[0042] The specific preparation steps of the cleaned olivine powder are as follows: take natural olivine powder, crush and grind it, pass it through a 60-mesh nylon sieve, wash away surface impurities with ultrapure water, and dry it in an oven at 50℃ for 24 hours. After drying, it can directly replace the modified olivine powder in the preparation of functional organic fertilizer.
[0043] Comparative Example 4 The only difference between the composition and preparation method of a functional organic fertilizer and Example 2 is that in the modified olivine powder preparation method: natural olivine powder is ground and passed through a 60-mesh nylon sieve. After washing away surface impurities with ultrapure water, it is dried in an oven at 50°C for 24 hours. After drying, it is calcined at high temperature in two stages: first at 200°C for 2 hours, and then at 450°C for 2 hours, to obtain modified olivine powder.
[0044] Comparative Example 5 A functional organic fertilizer, the preparation method of which is as follows: No other materials were added to the chicken manure. The chicken manure was composted and fermented at 25°C for 15 days to obtain the functional organic fertilizer of this comparative ratio.
[0045] Comparative Example 6 A functional organic fertilizer, by weight, consists of only 2 parts chicken manure and 1 part algae-bacteria symbiosis. The preparation method of the algae-bacteria symbiosis is the same as in Example 2.
[0046] Preparation method of functional organic fertilizer: Chicken manure and algae-bacteria symbiosis are mixed to obtain a mixed system. The initial moisture content of the mixed system is adjusted to 50%-55%, and fermented at 25℃ for 15 days. The mixture is turned over every two days from the 5th to the 15th day after the start of fermentation to obtain functional organic fertilizer.
[0047] Comparative Example 7 A functional organic fertilizer, composed solely of modified olivine powder, prepared using the same method as in Example 2.
[0048] Comparative Example 8 A functional organic fertilizer, the only difference in composition and preparation method from Example 2 is that chicken manure is replaced with sheep manure.
[0049] Comparative Example 9 A functional organic fertilizer, the only difference in composition and preparation method from Example 2 is that chicken manure is replaced with cow manure.
[0050] Comparative Example 10 The only difference between the composition and preparation method of the functional organic fertilizer and Example 2 is that the modified olivine powder is first calcined at 200°C for 2 hours and then calcined at 600°C for 2 hours.
[0051] Comparative Example 11 The only difference between the composition and preparation method of the functional organic fertilizer and Example 2 is that the modified olivine powder is first calcined at 200°C for 2 hours and then calcined at 300°C for 2 hours.
[0052] Comparative Example 12 A functional organic fertilizer, the only difference between its composition and preparation method and Example 2 is that the modified olivine powder is calcined at 450°C for 2 hours.
[0053] Comparative Example 13 A functional organic fertilizer, the only difference in composition and preparation method from Example 2 is that the modified olivine powder is calcined at 200°C for 2 hours.
[0054] Example 1: The effect of functional organic fertilizer on greenhouse gas emission reduction in tomato cultivation The organic fertilizers prepared in Examples 1-3 and Comparative Examples 1-13 were applied to a tomato seed germination experiment, and were set up as blank group 1, blank group 2, experimental group 1, experimental group 2, experimental group 3, control group 1, control group 2, control group 3, control group 4, control group 5, control group 6, control group 7, control group 8, control group 9, control group 10, control group 11, control group 12, and control group 13, respectively. The same management mode was used, with the following differences: Blank group 1 received no organic fertilizer; Blank group 2 received Huano vegetable-specific organic fertilizer (purchased from the Taobao store "Huano Flagship Store"); Experiment group 1 received the organic fertilizer prepared in Example 1; Experiment group 2 received the organic fertilizer prepared in Example 2; Experiment group 3 received the organic fertilizer prepared in Example 3; Control group 1 received the organic fertilizer prepared in Comparative Example 1; Control group 2 received the organic fertilizer prepared in Comparative Example 2; Control group 3 received the organic fertilizer prepared in Comparative Example 3; Control group 4 received the organic fertilizer prepared in Comparative Example 4; Control group 5 received the organic fertilizer prepared in Comparative Example 5; Control group 6 received the organic fertilizer prepared in Comparative Example 6; and so on; Control group 13 received the organic fertilizer prepared in Comparative Example 13.
[0055] Except for the blank group, all experimental groups were treated with fertilizer at a rate of 80 kg / mu before planting tomatoes.
[0056] Greenhouse cultivation experiments were conducted on the above-mentioned groups of tomatoes. Gas samples were collected from the tomato plants at 9:00 AM on days 20 (seedling stage), 90 (flowering and fruiting stage), and 130 (maturity stage) after planting, totaling 20 mL. The gas composition was measured, and the greenhouse gas emission rate of each group was calculated based on the CO2 concentration in the gas samples. The calculation formula is as follows: The CO2 emission rate calculation formula is shown below: R (CO2) =( ×C×V×273) / [m×(273+T)×t]; R (CH4) =( ×C×V×273) / [m×(273+T)×t]; R (N2O) =( ×C×V×273) / [m×(273+T)×t]; Among them, R (CO2) R (CH4) and R (N2O) The emission rates of the measured gases are respectively expressed in mg·kg⁻¹. -1 ·d -1 ρ is the density of the gas being measured under standard conditions (CO2 = 1.97 kg / m³). 3 CH4 = 0.717 kg / m³ 3 N2O = 1.977 kg / m³ 3 C represents the concentration of the gas being measured, in mL / m³. 3 V represents the effective space of the gas in the gas collecting chamber, m 3 m is the weight of the dry soil in the gas collection chamber, kg; T is the temperature of the gas being measured, °C; t is the incubation time, d.
[0057] The results of greenhouse gas emission rates during tomato cultivation are shown in Tables 1, 2, and 3. According to Tables 1-3, compared to the two blank groups treated with commercially available organic fertilizer, the emission rates of CO2, CH4, and N2O from tomato plants treated with the functional organic fertilizer prepared in Examples 1-3 of this invention (Experimental Groups 1-3) were significantly reduced at each planting stage. Compared to Experimental Groups 1-3, the emission rates of CO2, CH4, and N2O from tomato plants treated with the functional organic fertilizer prepared in Comparative Examples 1-13 of this invention (Control Groups 1-13) were significantly increased at each planting stage. This indicates that the optimized functional organic fertilizer preparation method of this invention is key to effectively reducing greenhouse gas emission rates during tomato cultivation.
[0058] Table 1. Carbon dioxide emission rate during tomato cultivation Table 2. Methane emission rates during tomato cultivation Table 3. Nitrous oxide emission rate during tomato growing season Example 2: Effect Verification: The Promotion of Tomato Quality by Functional Organic Fertilizer The organic fertilizers prepared in Examples 1-3 and Comparative Examples 1-13 were applied to tomato seed germination experiments, divided into four groups: blank group 1, blank group 2, experimental group 1, experimental group 2, experimental group 3, control group 1, control group 2, control group 3, control group 4, control group 5, control group 6, control group 7, control group 8, control group 9, control group 10, control group 11, control group 12, and control group 13. All groups used the same management model, with the following differences: Blank group 1 received no organic fertilizer; Blank group 2 received Huano vegetable-specific organic fertilizer; Experiment group 1 received the organic fertilizer prepared in Example 1; Experiment group 2 received the organic fertilizer prepared in Example 2; Experiment group 3 received the organic fertilizer prepared in Example 3; Control group 1 received the organic fertilizer prepared in Comparative Example 1; Control group 2 received the organic fertilizer prepared in Comparative Example 2; Control group 3 received the organic fertilizer prepared in Comparative Example 3; Control group 4 received the organic fertilizer prepared in Comparative Example 4; Control group 5 received the organic fertilizer prepared in Comparative Example 5; Control group 6 received the organic fertilizer prepared in Comparative Example 6; and so on; Control group 13 received the organic fertilizer prepared in Comparative Example 13.
[0059] Except for the blank group, all experimental groups were treated with fertilizer at a rate of 80 kg / mu before planting tomatoes.
[0060] A greenhouse cultivation experiment was conducted on the above-mentioned groups of tomatoes. On day 130 after planting, mature tomatoes and plant leaves were collected, and the dry biomass, soluble sugar content, total acid content, sugar-acid ratio, vitamin C content, and chlorophyll content of the plant leaves were measured. The results are shown in Table 4.
[0061] As shown in Table 4, compared with the blank group 1 (without any fertilizer) or the blank group 2 (with commercially available organic fertilizer), the tomato plants (experimental groups 1-3) treated with the functional organic fertilizer prepared in Examples 1-3 of this invention showed significantly increased fruit dry biomass, soluble sugar content, sugar-acid ratio, vitamin C content, and leaf chlorophyll content, and significantly decreased total acid content. Compared with experimental groups 1-3, the tomato plants (control groups 1-13) treated with the functional organic fertilizer prepared in Comparative Examples 1-13 of this invention showed significantly decreased fruit dry biomass, soluble sugar content, sugar-acid ratio, vitamin C content, and leaf chlorophyll content, and significantly increased total acid content. This indicates that the optimized preparation method of the functional organic fertilizer of this invention is key to effectively improving the quality of tomato fruits and increasing leaf chlorophyll content.
[0062] Table 4. Experimental results of tomato ripening fruit indicators and chlorophyll content in plant leaves. The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.
Claims
1. A method for preparing a functional organic fertilizer, characterized in that, Includes the following steps: The functional organic fertilizer is obtained by mixing algae-bacteria symbiosis, modified olivine powder and chicken manure and then fermenting the mixture.
2. The preparation method according to claim 1, characterized in that, The mass ratio of the algae-bacteria symbiosis, the modified olivine powder, and the chicken manure is 1:(1-2):(2-4).
3. The preparation method according to claim 1, characterized in that, The algae-bacterial symbiotic was obtained by inoculating Chlorella and Streptomyces flavus into a culture medium and then co-amplifying and culturing them.
4. The preparation method according to claim 3, characterized in that, The amplification culture is subjected to a light exposure of no less than 12 h / d, a light intensity of 5000-5400 Lux, a temperature of 26℃, and a duration of 12 days.
5. The preparation method according to claim 1, characterized in that, The modified olivine powder is obtained by crushing, drying, and calcining olivine powder.
6. The preparation method according to claim 5, characterized in that, The drying temperature shall not exceed 40°C and the time shall be 24 hours. The calcination includes calcination at 200℃ for 2 hours and calcination at 450℃ for 2 hours.
7. The preparation method according to claim 1, characterized in that, The fermentation temperature is 25-35℃, and the time is 10-15 days.
8. A functional organic fertilizer obtained by the preparation method according to any one of claims 1-7.
9. The application of the functional organic fertilizer as described in claim 8 in mitigating greenhouse gas emissions from tomatoes.
10. The application of the functional organic fertilizer as described in claim 8 in improving the quality of tomato fruits and / or leaves.