A method for inducing differentiation of human pluripotent stem cells into bone marrow stromal cells and use thereof

By differentiating human pluripotent stem cells into endothelial cells and inducing endothelial-mesenchymal transition using TGF-β, the problem of obtaining bone marrow stromal cells has been solved. The obtained BMSCs have a stable phenotype and mature function, promote the proliferation and differentiation of hematopoietic stem cells, delay aging, and have broad clinical application potential.

CN122168514APending Publication Date: 2026-06-09THE HONG KONG POLYTECHNIC UNIV SHENZHEN RES INST
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
THE HONG KONG POLYTECHNIC UNIV SHENZHEN RES INST
Filing Date
2026-02-06
Publication Date
2026-06-09

AI Technical Summary

Technical Problem

In existing technologies, the acquisition of bone marrow stromal cells has problems such as invasive operations, limited donor sources, low purity, and high heterogeneity. In addition, the differentiation technology of human pluripotent stem cells is not efficient, making it difficult to obtain phenotypically mature and functionally complete BMSCs, which limits their preparation and application on a clinical scale.

Method used

By simulating the physiological development process, human pluripotent stem cells are first differentiated into endothelial cells, and then TGF-β is used to induce endothelial-mesenchymal transition (EndMT) to efficiently obtain phenotypically stable and functionally mature bone marrow stromal cells. CD31+ endothelial cells are used as precursors, and the TGF-β-induced endothelial-mesenchymal transition (EndMT) pathway is utilized to achieve efficient and stable preparation of BMSCs.

Benefits of technology

The obtained bone marrow stromal cells have a stable phenotype and low heterogeneity, which can effectively promote the proliferation and multi-lineage differentiation of hematopoietic stem cells, delay aging, and have a significant ability to promote the expansion of immune cells. They have good application prospects in the fields of hematopoietic reconstitution, intervention of aging-related blood diseases, and immunomodulatory therapy.

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Abstract

This invention discloses a method and application for inducing human pluripotent stem cells to differentiate into bone marrow stromal cells, relating to the field of biomedical technology. The induction method of this invention includes: firstly, obtaining human pluripotent stem cells, and then inducing their differentiation into endothelial cells; then, using TGF-β to induce the endothelial cells to differentiate into bone marrow stromal cells, thus obtaining the cells. Bone marrow stromal cells obtained based on the induction method of this invention can not only effectively promote the expansion and multi-lineage differentiation of hematopoietic stem cells and reverse the aging phenotype of senescent hematopoietic stem cells, but also promote the proliferation of immune cells such as T cells and NK cells, showing great application potential in hematopoietic reconstitution, immune regulation, and anti-aging intervention.
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