A self-closing porous filter device

Abstract

The application discloses a self-closing porous filtering device, which comprises a sample plate and a recovery plate. At least one sample plate hole pipe is arranged on the sample plate, and at least one recovery plate hole pipe is arranged on the recovery plate. The sample plate is arranged above the recovery plate and is connected to the recovery plate in a self-locking embedded mode. When the sample plate and the recovery plate are embedded, the sample plate hole pipe is arranged in the recovery plate hole pipe in a sealed mode. When the sample is heated and shaken for pretreatment, the sample plate hole pipe is arranged in the recovery plate hole pipe in a sealed mode after the sample plate and the recovery plate are embedded in a self-locking mode, and the top of the sample plate is sealed with a film, so that liquid leakage, reagent volatilization and sample pollution are avoided, and the safety of the sample, personnel, equipment and environment is effectively ensured. When centrifugal filtration is needed, the locking can be released, the recovery plate and the sample plate are separated, and liquid is recovered through centrifugal filtration, so that high-throughput, automation, no pollution, reduced inspection cost and improved efficiency are achieved.

Description

Technical Field

[0001] This invention belongs to the field of biological equipment technology, specifically relating to a self-sealing porous filtration device. Background Technology

[0002] In laboratories specializing in forensic science, clinical testing, animal testing, and disease control, it is necessary to process solid samples (such as fecal swabs, exfoliated cell extractors, and environmental sampling swabs) to detect macromolecular target substances such as proteins and nucleic acids. Sample lysis or washing and release is essential. To obtain as many samples as possible, centrifugation to remove the carrier is an indispensable step. After centrifugation, the lysate or sample leachate is aspirated for downstream extraction and purification.

[0003] The current mainstream technique for pretreatment of solid samples is to use manual centrifuge tubes. Because it is necessary to seal the tubes to prevent liquid evaporation from affecting the laboratory environment and personnel, the container containing the sample needs to be embedded in the centrifuge recovery container. After centrifugation, the inner tube containing the sample carrier and the outer tube containing the centrifugation recovery product need to be separated in order to remove the lysis products.

[0004] Sample lysis typically requires heating. Due to the need for centrifugation for recovery, an opening must be left at the bottom. Therefore, sample containers cannot be completely sealed during lysis to prevent liquid from overflowing due to increased internal pressure. Unsealed lysis platens are prone to liquid evaporation and contamination, adversely affecting the laboratory environment and personnel. If the volatile reagents are corrosive, evaporation from unsealed sample tubes during automated processing may shorten the lifespan of the equipment.

[0005] Currently, most sample containers used for processing these types of samples are manual centrifuge sleeves, which are not conducive to automation. While the AutoLys sleeves produced by Hamiltion in Switzerland can achieve automation, they require specialized robotic arms, resulting in high equipment and consumable costs, low processing efficiency, and limited application. Automated equipment with low reliance on hardware uses SBS-format multi-well sample plates for sample lysis, which cannot achieve full-plate coating or sealing, easily leading to liquid evaporation and aerosol contamination.

[0006] Therefore, a high-throughput, automated, pollution-free, and cost-reducing self-sealing porous filtration device is urgently needed. Summary of the Invention

[0007] To address the shortcomings of existing technologies, this invention provides a self-sealing porous filtration device.

[0008] To solve the above-mentioned technical problems, the present invention provides the following technical solution:

[0009] The present invention provides a self-sealing porous filter device, including a sample plate and a recovery plate. The sample plate is provided with at least one sample plate perforated tube, and the recovery plate is provided with at least one recovery plate perforated tube. The sample plate is disposed above the recovery plate and the two are self-lockingly connected. When the sample plate and the recovery plate are fitted together, the sample plate perforated tube is sealed in the recovery plate perforated tube.

[0010] Preferably, the sample plate has feet around its perimeter, and self-locking surfaces are provided on both sides along its length. The self-locking surfaces are recessed inward from the two sides along the length of the sample plate. The self-locking surfaces are provided with locking holes, guide surfaces, and anti-slip lines. The guide surfaces are located below the locking holes, and the anti-slip lines are located above the locking holes. The guide surfaces are recessed inward from the self-locking surfaces.

[0011] Preferably, the sample plate has opening slots on both sides along its length and below the self-locking surface for the robotic arm to grasp and recycle the plate.

[0012] Preferably, the recycling plate has elastic buckles on both sides along its length, and the elastic buckles are self-lockingly engaged with the buckle holes.

[0013] Preferably, the lower inner wall of the sample plate perforated tube is provided with a welding line for welding the filter membrane or filter screen plate, and a support piece for supporting the filter membrane or filter screen plate is provided on the lower inner wall of the sample plate perforated tube below the welding line.

[0014] Preferably, the lower outer wall of the sample plate tube, located below the welding line, is a conical surface of the sample tube. The conical surface of the sample tube is provided with a limiting groove or covered with a flexible sealing adhesive, and a first sealing ring is fixed in the limiting groove.

[0015] Preferably, the lower part of the recycling plate perforated tube is provided with a recycling tube conical surface, and when the sample plate and the recycling plate are fitted together, the recycling tube conical surface is located outside the sample tube conical surface.

[0016] Preferably, the two sides of the sample plate and the recycling plate along their length are connected one-to-one by a mortise and tenon self-locking structure.

[0017] Preferably, the top outer wall of the sample plate orifice tube is sealed to the top inner wall of the recovery plate orifice tube by a second sealing ring, and the bottom outer wall of the sample plate orifice tube is sealed to the bottom inner wall of the recovery plate orifice tube by a sealing sheet.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] This invention, during sample heating and shaking pretreatment, involves a self-locking mechanism where the sample plate and recovery plate are fitted together using elastic latches and locking holes, or a tenon-and-mortise self-locking structure. The sample plate's perforated tube is sealed within the recovery plate's perforated tube, and a sealing film is applied to the top of the sample plate. This prevents leakage of bottom liquid, reagent evaporation, and sample contamination, effectively protecting the safety of samples, personnel, equipment, and the environment. When centrifugation filtration is required, the locking mechanism can be released mechanically or manually, releasing the bottom seal. The previously locked recovery plate can then move downwards, separating itself from the sample plate, ensuring the bottom of the recovery plate and the bottom of the sample plate are at the same level. Centrifugation then recovers the liquid from the sample plate's perforated tube. This allows for easy automation, achieving high throughput, automation, zero contamination, reduced testing costs, and increased efficiency. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the overall structure of a self-sealing porous filter device according to the present invention.

[0021] Figure 2 This is a schematic diagram of the sample plate structure in Embodiment 1 of the present invention;

[0022] Figure 3 This is a schematic diagram of the structure of the recycling plate in Embodiment 1 of the present invention;

[0023] Figure 4 This is a cross-sectional view of the sample plate perforated tube in Embodiment 1 of the present invention;

[0024] Figure 5 This is a cross-sectional view of the sample plate perforated tube in Embodiment 2 of the present invention;

[0025] Figure 6 This is a cross-sectional view of the recycling plate in Embodiment 1 of the present invention;

[0026] Figure 7 This is a schematic diagram of the self-locking interlocking connection between the sample plate and the recycling plate in Embodiment 1 of the present invention;

[0027] Figure 8 This is a schematic diagram of the sealed connection between the sample plate perforated tube and the recovery plate perforated tube in Embodiment 1 of the present invention;

[0028] Figure 9 This is a schematic diagram of the action of the sample plate and the recycling plate releasing the latch in Embodiment 1 of the present invention;

[0029] Figure 10 This is a diagram showing the state of the sample plate and the recycling plate after the locking mechanism is released in Embodiment 1 of the present invention.

[0030] Figure 11 This is an enlarged schematic diagram of the self-locking interlocking connection between the sample plate and the recycling plate in Embodiment 1 of the present invention;

[0031] Figure 12 This is an enlarged schematic diagram of the self-locking interlocking connection between the sample plate and the recycling plate in Embodiment 2 of the present invention;

[0032] Figure 13 This is an enlarged schematic diagram of the sealed connection between the sample plate perforated tube and the recovery plate perforated tube in Embodiment 1 of the present invention. Detailed Implementation

[0033] The preferred embodiments of the present invention will be described below with reference to the accompanying drawings. It should be understood that the preferred embodiments described herein are for illustration and explanation only and are not intended to limit the present invention.

[0034] In the description of this invention, it should be understood that the terms "left" and "right" and other terms indicating orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings and are only for the convenience of describing this invention and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this invention.

[0035] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0036] Example 1

[0037] like Figures 1 to 4 As shown, this embodiment provides a self-sealing porous filter device, including a sample plate 1-1 and a recovery plate 1-2. The sample plate 1-1 is provided with 24 sample plate perforated tubes, and the recovery plate 1-2 is provided with 24 recovery plate perforated tubes. The sample plate 1-1 is disposed above the recovery plate 1-2 and the two are self-lockingly fitted together. When the sample plate 1-1 and the recovery plate 1-2 are fitted together, the sample plate perforated tubes are sealed in the recovery plate perforated tubes.

[0038] like Figure 2 As shown, the sample plate 1-1 is provided with feet 2-1 around its perimeter. The sample plate 2-1 has self-locking surfaces on both sides along its length. The self-locking surfaces are recessed inward from the two sides along the length of the sample plate. The self-locking surfaces are provided with locking holes 2-2, guide surfaces 2-3 and anti-slip lines 2-4. The guide surfaces 2-3 are located below the locking holes 2-2, and the anti-slip lines 2-4 are located above the locking holes 2-2. The guide surfaces 2-3 are recessed inward from the self-locking surfaces.

[0039] In this embodiment, the sample plate 1-1 has opening slots on both sides along its length and below the self-locking surface for a robotic arm to grasp and recycle the plate.

[0040] like Figure 3 , Figure 6 As shown, the recycling plate 1-2 has elastic latches 3-1 on both sides along its length, and the elastic latches 3-1 are self-lockingly engaged with the latch holes 2-2. The elastic latches are made of SBS.

[0041] like Figure 4 As shown, the lower inner wall of the sample plate perforated tube is provided with a welding line 4-1 for welding the filter membrane or filter screen plate, and a support piece 4-2 for supporting the filter membrane or filter screen plate is provided on the lower inner wall of the sample plate perforated tube and below the welding line.

[0042] like Figure 4 As shown, the lower outer wall of the sample plate tube, located below the welding line 4-1, is a conical surface of the sample tube. A limiting groove 4-3 is provided on the conical surface of the sample tube, and a first sealing ring is fixed in the limiting groove 4-3.

[0043] like Figure 8 As shown, the lower part of the recycling plate tube is provided with a recycling tube conical surface 6-1. When the sample plate 1-1 and the recycling plate 1-2 are fitted together, the recycling tube conical surface 6-1 is located outside the sample tube conical surface.

[0044] like Figure 13 As shown, the top outer wall of the sample plate orifice tube is sealed to the top inner wall of the recovery plate orifice tube by a second sealing ring 13-1, and the bottom outer wall of the sample plate orifice tube is sealed to the bottom inner wall of the recovery plate orifice tube by an elastic silicone sealing sheet 13-2.

[0045] The working principle of this embodiment will be further explained below:

[0046] like Figures 6 to 11 As shown, during sample heating and shaking pretreatment, the sample plate and recovery plate are self-lockingly fitted together using elastic latches and lock holes, or a tenon-and-mortise self-locking structure. The sample plate's perforated tube is sealed within the recovery plate's perforated tube, and then a sealing film is applied to the top of the sample plate. This prevents bottom liquid leakage, reagent evaporation, and sample contamination, effectively protecting the safety of samples, personnel, equipment, and the environment. When centrifugation filtration is required, the locking mechanism can be released mechanically or manually, releasing the bottom seal. The previously locked recovery plate can then move downwards, separating itself from the sample plate, ensuring the bottom of the recovery plate and the sample plate are at the same level. The liquid within the sample plate's perforated tube can then be recovered by centrifugation. This allows for easy automation, achieving high throughput, automation, zero contamination, reduced testing costs, and increased efficiency.

[0047] Example 2

[0048] like Figure 5 As shown, the rest is the same as in Example 1, except that the conical surface and bottom outlet of the sample tube are covered with flexible sealing adhesive 5-1.

[0049] Example 3

[0050] like Figure 12 As shown, the rest is the same as in Example 1, except that the two sides of the sample plate 1-1 and the recycling plate 1-2 in the length direction are connected one-to-one by a mortise and tenon self-locking structure.

[0051] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A self-sealing porous filter device, characterized in that, It includes a sample plate (1-1) and a recycling plate (1-2). The sample plate (1-1) is provided with at least one sample plate perforated tube, and the recycling plate (1-2) is provided with at least one recycling plate perforated tube. The sample plate (1-1) is located above the recycling plate (1-2) and the two are self-lockingly fitted together. When the sample plate (1-1) and the recycling plate (1-2) are fitted together, the sample plate perforated tube is sealed in the recycling plate perforated tube.

2. The self-sealing porous filter device according to claim 1, characterized in that, The sample plate (1-1) is provided with feet (2-1) around its perimeter. The sample plate (2-1) has self-locking surfaces on both sides along its length. The self-locking surfaces are recessed inward from the two sides along the length of the sample plate. The self-locking surfaces are provided with a locking hole (2-2), a guide surface (2-3), and an anti-slip line (2-4). The guide surface (2-3) is located below the locking hole (2-2), and the anti-slip line (2-4) is located above the locking hole (2-2). The guide surface (2-3) is recessed inward from the self-locking surface.

3. The self-sealing porous filter device according to claim 2, characterized in that, The sample plate (1-1) has opening slots on both sides along its length and below the self-locking surface for a robotic arm to grasp and recycle the plate.

4. The self-sealing porous filter device according to claim 3, characterized in that, The recycling plate (1-2) has elastic buckles (3-1) on both sides along its length, and the elastic buckles (3-1) are self-lockingly engaged with the buckle holes (2-2).

5. The self-sealing porous filter device according to claim 4, characterized in that, The lower inner wall of the sample plate perforated tube is provided with a welding line (4-1) for welding the filter membrane or filter screen plate, and a support piece (4-2) for supporting the filter membrane or filter screen plate is provided on the lower inner wall of the sample plate perforated tube and below the welding line.

6. The self-sealing porous filter device according to claim 5, characterized in that, The lower outer wall of the sample plate tube, located below the welding line (4-1), is a conical surface of the sample tube. The conical surface of the sample tube is provided with a limiting groove (4-3) or covered with a flexible sealing adhesive (5-1). A first sealing ring is fixed in the limiting groove (4-3).

7. The self-sealing porous filter device according to claim 6, characterized in that, The lower part of the recycling plate is provided with a recycling tube conical surface (6-1). When the sample plate (1-1) and the recycling plate (1-2) are fitted together, the recycling tube conical surface (6-1) is located outside the sample tube conical surface.

8. The self-sealing porous filter device according to claim 1, characterized in that, The sample plate (1-1) and the recycling plate (1-2) are connected one-to-one along their length by a mortise and tenon self-locking structure.

9. The self-sealing porous filter device according to any one of claims 1-8, characterized in that, The top outer wall of the sample plate orifice tube is sealed to the top inner wall of the recovery plate orifice tube by a second sealing ring (13-1), and the bottom outer wall of the sample plate orifice tube is sealed to the bottom inner wall of the recovery plate orifice tube by a sealing piece (13-2).

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