Electrode material for measuring oxygen content in urine and preparation method and application thereof

Abstract

The application relates to the field of electrochemistry, in particular to an electrode material for measuring oxygen content in urine and a preparation method and application thereof. The electrode material comprises MoC and Pt, and the Pt is loaded on the surface of the MoC. The method has the advantages that the utilization efficiency of the noble metal Pt is improved by using the MoC carrier; the MoC-Pt composite system shows sensitivity to O2 sensing in liquid and strong anti-interference ability to common small molecules and ions in body fluid; and the preparation cost can be greatly reduced compared with a metal Pt wire or Pt sheet electrode.

Description

Technical Field

[0001] This invention relates to the field of electrochemical technology, and in particular to an electrode material for measuring the oxygen content of urine, its preparation method, and its application. Background Technology

[0002] In the fields of clinical diagnosis and intensive care, accurate detection of urinary oxygen content is of significant reference value for assessing the body's metabolic status, renal function, and circulatory perfusion, especially for monitoring the condition and predicting the prognosis of critically ill patients. Currently, electrochemical electrode detection is the "gold standard" for measuring urinary oxygen content and is also the most directly applied core technology. While academia and industry are exploring various alternative technological pathways, the existing technological system still faces many bottlenecks that urgently need to be addressed, limiting its routine clinical application.

[0003] The current mainstream detection method is electrochemical electrode detection. Electrochemical oxygen detection electrodes typically employ a composite electrode structure, with a core consisting of a noble metal cathode (such as platinum, gold, or other inert metals) and a silver / silver chloride anode. Both electrodes are immersed in a specific electrolyte solution, forming a complete electrochemical reaction system. To achieve specific detection of oxygen, the electrode tip is encapsulated with a functional thin film that selectively permeates oxygen. Common materials include polytetrafluoroethylene (PTFE) and polyethylene. This film effectively blocks impurities such as proteins and crystals in urine, allowing only oxygen molecules to permeate. During detection, a constant polarization voltage is applied across the electrode. Oxygen molecules that have permeated the film undergo an irreversible reduction reaction on the cathode surface, generating a weak redox current. The intensity of this current is strictly linearly proportional to the partial pressure of oxygen in the sample. By using a pre-established calibration curve, the oxygen content in the urine can be accurately calculated.

[0004] Although electrochemical electrode methods and various alternative techniques have provided feasible pathways for urine oxygen content detection, urine, as a special sample with complex composition and highly variable physicochemical properties, presents many challenges in its application compared to blood tests. These issues have collectively limited urine oxygen content detection to research settings and exploratory applications in a few intensive care units, preventing it from becoming a routine, standardized clinical laboratory test like blood gas analysis. The specific bottlenecks can be summarized into the following four categories: First, the problem of biofouling: When sensors (especially the sensitive membrane of electrochemical electrodes and the surface of fiber optic fluorescent probes) are immersed in urine for a long time, the proteins, urates, phosphates and other components in the urine will quickly deposit to form biofilms or crystal layers. The deposition rate is much faster than that in the blood environment. Usually, within a few hours, it can cause serious drift of sensor signals, decreased sensitivity, or even complete failure, which cannot meet the needs of long-term continuous monitoring.

[0005] Second, chemical pollution and toxicity issues: High concentrations of urea, creatinine, and some drug metabolites in urine may penetrate the selective permeability membrane of the sensor and undergo irreversible chemical reactions with the electrolyte solution of the electrode and the noble metal of the cathode, or cause chemical damage to the fluorescent probe and electrochemiluminescent material, resulting in a permanent decrease in sensor sensitivity and a shortened lifespan.

[0006] Third, the problem of bubble interference: The surfaces of detection elements such as electrochemical electrode sensitive membranes and fiber optic probes have poor wettability. Temperature changes and agitation of urine during drainage can easily cause dissolved oxygen and other gases in the urine to precipitate, forming tiny bubbles. These bubbles easily adhere to the surface of the detection elements, blocking the contact between oxygen and the sensitive elements, causing drastic fluctuations in detection readings, a significant decrease in accuracy, and even detection interruption.

[0007] Fourth, practicality and commercialization are limited: Ex vivo testing methods have inherent defects such as high time sensitivity, cumbersome operation procedures, and inability to conduct continuous monitoring, making it difficult to meet the needs of real-time clinical diagnosis and treatment; In vivo continuous monitoring technology faces multiple technical challenges such as poor long-term stability, difficulty in in-situ calibration, high cost of core materials, and insufficient biocompatibility, resulting in a lack of commercial products; and the "off-label" blood testing device method has problems such as high safety risks, insufficient accuracy, and limited operation.

[0008] In summary, the existing urine oxygen content detection technology system has significant shortcomings in terms of detection stability, practicality, safety, and commercialization. There is an urgent need to overcome these technical bottlenecks by developing highly sensitive new materials, innovating sensing principles, and optimizing in vivo integrated design, so as to promote the routine clinical application of urine oxygen content detection technology.

[0009] Currently, in response to the aforementioned core problems in related technologies, the industry has not yet proposed an electrochemical detection solution that overcomes the instability, low effectiveness, and high cost of electrochemical electrodes in urine sample detection. Summary of the Invention

[0010] The purpose of this application is to address the shortcomings of the prior art by providing an electrode material, preparation method, working electrode, electrode device, and urine oxygen content measurement system for measuring urine oxygen content, so as to at least solve the problems of instability, low effectiveness, and high cost of electrochemical electrodes for urine sample detection in related technologies.

[0011] To achieve the above objectives, the technical solution adopted in this application is as follows: In a first aspect, an electrode material for measuring the oxygen content of urine is provided, comprising MoC and Pt, wherein Pt is loaded on the surface of MoC.

[0012] In some of these embodiments, MoC is a nanoscale sheet structure and Pt is a nanoscale particle structure.

[0013] In some of these embodiments, the mass loading of Pt is 6% to 15%.

[0014] In a second aspect, a method for preparing an electrode material for measuring the oxygen content of urine is provided, for preparing the electrode material as described in the first aspect, comprising: Electrode materials were prepared by immersion heating of MoC and Pt.

[0015] In some of these embodiments, the following are included: MoC, chloroplatinic acid hexahydrate, and deionized water are mixed in a preset ratio to obtain a uniformly dispersed suspension. The suspension is hydrothermally heated at a preset temperature to obtain the reaction liquid. The reaction liquid is subjected to solid-liquid separation to obtain electrode materials.

[0016] In some of these embodiments, MoC, chloroplatinic acid hexahydrate, and deionized water are ultrasonically mixed in a preset ratio to obtain a uniformly dispersed suspension.

[0017] In some of these embodiments, the preset temperature is 50~90°C.

[0018] In some of these embodiments, the mixing time is 0.5 to 6 hours.

[0019] In some of these embodiments, solid-liquid separation includes filtration, washing, and drying.

[0020] Thirdly, a working electrode for measuring urine oxygen content is provided, which is prepared from the electrode material described in the first aspect, or from the electrode material prepared by the preparation method described in the second aspect.

[0021] Fourthly, an electrode device for measuring the oxygen content of urine is provided, comprising: The working electrode as described in the third aspect; A reference electrode, wherein the reference electrode is disposed on one side of the working electrode; The counter electrode is disposed on one side of the working electrode and the reference electrode.

[0022] In some of these embodiments, it also includes: A substrate, wherein the working electrode, the reference electrode, and the counter electrode are disposed on the surface of the substrate; A first conductive contact plate is disposed on the surface of the substrate; The second conductive contact plate is disposed on the surface of the substrate; A third conductive contact plate is disposed on the surface of the substrate; The first conductive wire is connected to the working electrode and the first conductive contact plate respectively, and is used to transmit current. The second conductive wire is connected to the reference electrode and the second conductive contact plate respectively, and is used to transmit current. The third conductive wire is connected to the counter electrode and the third conductive contact plate respectively, and is used to transmit current.

[0023] Fifthly, a urine oxygen content measurement system is provided, comprising: The electrode device as described in the fourth aspect; A urine oxygen content detection device, which is connected to the electrode device, is used to measure urine oxygen content.

[0024] Compared with related technologies, the electrode material, preparation method, working electrode, electrode device, and urine oxygen content measurement system provided in this application have the following technical advantages: (1) Improve the utilization efficiency of precious metal Pt by using MoC support; (2) The MoC-Pt composite system demonstrated sensitivity to O2 sensing in liquids and strong anti-interference ability against common small molecules and ions in body fluids. (3) Compared with metal Pt wire or Pt sheet electrodes, the manufacturing cost can be greatly reduced. Attached Figure Description

[0025] The accompanying drawings, which are included to provide a further understanding of this application and form part of this application, illustrate exemplary embodiments and are used to explain this application, but do not constitute an undue limitation of this application. In the drawings: Figures 1-2 This is a schematic diagram of an electrode device according to an embodiment of the present invention; Figure 3 This is a schematic diagram of a urine oxygen content measurement system according to an embodiment of the present invention; Figure 4 These are SEM and TEM images of the MoC-Pt electrode according to Embodiment 2 of the present invention, wherein (a) is an SEM image and (b) is a TEM image; Figure 5These are the XRD patterns of the MoC-Pt electrodes according to Embodiments 2-6 of the present invention, wherein MoC JCPDS#45-1015 is the standard powder diffraction card number of the β-MoC phase of MoC (XRD standard data of the β-MoC phase), and Pt JCPDS#87-0640 is the standard powder diffraction card number of Pt (XRD standard data of Pt). It should be noted that JCPDS is an abbreviation for Joint Committee on Powder Diffraction Standards, now renamed International Centre for Diffraction Data (ICDD). In addition, in the ICDD database, MoC JCPDS#45-1015 corresponds to MoC PDF#45-1015, and Pt JCPDS#87-0640 corresponds to Pt PDF#87-0640. Figure 6 The image shows the electrochemical reduction CV of the MoC-Pt electrode prepared according to Example 2 of the present invention in N2-saturated and O2-saturated 1M PBS solutions; Figure 7 The CV curve and peak current-oxygen content fitting curve are obtained by continuously adding 1 mL of saturated O2 concentration PBS solution to 15 mL of N2 saturated PBS solution for the MoC-Pt electrode prepared according to Example 2 of the present invention. Among them, (a) is the CV curve and (b) is the peak current-oxygen content fitting curve. Figure 8 The it curve and current-oxygen content fitting curve are obtained by continuously adding 1 mL of saturated O2 concentration PBS solution to 15 mL of N2 saturated PBS solution for the MoC-Pt electrode prepared according to Example 2 of the present invention. Among them, (a) is the it curve and (b) is the current-oxygen content fitting curve. Figure 9 The it curves of the MoC-Pt electrode prepared according to Example 2 of the present invention with the addition of different interfering substances; Figure 10 The image shows a CV comparison of the MoC-Pt electrode and the MoC electrode prepared according to Example 2 of the present invention in PBS solution with saturated oxygen content.

[0026] The reference numerals in the figures are as follows: 111, working electrode; 112, first conductive contact plate; 113, first conductive line; 121, reference electrode; 122, second conductive contact plate; 123, second conductive line; 131, counter electrode; 132, third conductive contact plate; 133, third conductive line; 141, substrate; 142, cover plate; 143, first injection port; 144, intermediate plate; 145, second injection port; 146, flow channel; 147, third injection port. Detailed Implementation

[0027] To make the objectives, technical solutions, and advantages of this application clearer, the application is described and illustrated below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the scope of this application. All other embodiments obtained by those skilled in the art based on the embodiments provided in this application without inventive effort are within the scope of protection of this application.

[0028] Obviously, the accompanying drawings described below are merely some examples or embodiments of this application. Those skilled in the art can apply this application to other similar scenarios based on these drawings without any inventive effort. Furthermore, it is understood that although the efforts made in this development process may be complex and lengthy, for those skilled in the art related to the content disclosed in this application, any changes to design, manufacturing, or production based on the technical content disclosed in this application are merely conventional technical means and should not be construed as insufficient disclosure of the content of this application.

[0029] In this application, the reference to "embodiment" means that a specific feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places in the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment that is mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described in this application may be combined with other embodiments without conflict.

[0030] Unless otherwise defined, the technical or scientific terms used in this application shall have the ordinary meaning understood by one of ordinary skill in the art to which this application pertains. The terms “a,” “an,” “an,” “the,” and similar words used in this application do not indicate quantity limitation and may indicate singular or plural. The terms “comprising,” “including,” “having,” and any variations thereof used in this application are intended to cover non-exclusive inclusion; for example, a process, method, system, product, or device that includes a series of steps or modules (units) is not limited to the listed steps or units, but may also include steps or units not listed, or may include other steps or units inherent to these processes, methods, products, or devices. The terms “connected,” “linked,” “coupled,” and similar words used in this application are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. “Multiple” used in this application refers to two or more. “And / or” describes the relationship between related objects, indicating that three relationships may exist; for example, “A and / or B” can represent: A alone, A and B simultaneously, and B alone. The character " / " generally indicates that the preceding and following objects are in an "or" relationship. The terms "first," "second," and "third" used in this application are merely to distinguish similar objects and do not represent a specific ordering of the objects.

[0031] Example 1 This embodiment relates to electrode materials, preparation methods, working electrodes, electrode devices, and urine oxygen content measurement systems for measuring urine oxygen content.

[0032] An electrode material for measuring the oxygen content of urine includes MoC nanosheets and Pt nanoparticles. The Pt nanoparticles are loaded onto the surface of the MoC nanosheets.

[0033] In the above electrode materials, the mass loading of Pt is 6% to 15%.

[0034] The preparation method for the electrode material described above is as follows: MoC, chloroplatinic acid hexahydrate, and deionized water are ultrasonically mixed in a preset ratio (1-6 hours) to obtain a uniformly dispersed suspension. The suspension was hydrothermally heated at a preset temperature (50~90℃) for 0.5~6h to obtain the reaction liquid; The reaction liquid is subjected to solid-liquid separation to obtain electrode materials.

[0035] It should be noted that solid-liquid separation includes filtration, washing, and drying.

[0036] The electrode material described above has a larger specific surface area and stronger adsorption capacity compared to MoC material alone; compared to Pt material alone, it requires less Pt and has lower cost; in addition, the interaction between MoC and Pt enhances the reduction capacity of O2 and improves the electrical signal strength.

[0037] Using the electrode materials described above, a working electrode can be fabricated. This working electrode, in conjunction with other electrodes, can be used to measure the oxygen content in urine.

[0038] An electrode device can be fabricated using the working electrode described above. This electrode device can be used to measure the oxygen content in urine.

[0039] It should be noted that, for the working electrode as described above, it can be encapsulated by a thin film (such as polytetrafluoroethylene or polyethylene) that is selectively permeable to oxygen.

[0040] like Figure 1 As shown, the electrode device includes a working electrode 111, a reference electrode 121, and a counter electrode 131. The reference electrode 121 is disposed on one side of the working electrode 111, and the counter electrode 131 is disposed on one side of the working electrode 111 and the reference electrode 121.

[0041] It should be noted that the reference electrode 121 is an Ag / AgCl electrode.

[0042] It should be noted that the counter electrode 131 is a carbon electrode.

[0043] In some embodiments, the working electrode 111 is circular, the reference electrode 121 is arc-shaped, and the counter electrode 131 is arc-shaped. The reference electrode 121 is disposed around one side of the working electrode 111; the counter electrode 131 is disposed around one side of the working electrode 111 and is located on the side of the reference electrode 121.

[0044] It should be noted that the counter electrode 131 and the reference electrode 121 are not in contact.

[0045] It should be noted that the sum of the central angles of the counter electrode 131 and the reference electrode 121 is less than 360°. That is, the counter electrode 131 and the reference electrode 121 form a ring shape with two openings.

[0046] In some embodiments, the working electrode 111 is rectangular, the reference electrode 121 is rectangular or L-shaped, and the counter electrode 131 is rectangular or L-shaped. The reference electrode 121 is disposed around one side of the working electrode 111; the counter electrode 131 is disposed around one side of the working electrode 111 and is located on the side of the reference electrode 121.

[0047] It should be noted that the counter electrode 131 and the reference electrode 121 are not in contact.

[0048] It should be noted that at least a portion of the working electrode 111 is not surrounded by the reference electrode 121 and the counter electrode 131. That is, the counter electrode 131 and the reference electrode 121 form a rectangular ring shape with two openings.

[0049] Furthermore, such as Figure 1 As shown, the electrode device further includes a substrate 141, a first conductive contact plate 112, a second conductive contact plate 122, a third conductive contact plate 132, a first conductive wire 113, a second conductive wire 123, and a third conductive wire 133. The substrate 141 has a working electrode 111, a reference electrode 121, and a counter electrode 131 disposed on its surface; the first conductive contact plate 112 is disposed on the surface of the substrate 141; the second conductive contact plate 122 is disposed on the surface of the substrate 141; the third conductive contact plate 132 is disposed on the surface of the substrate 141; the first conductive wire 113 is connected to the working electrode 111 and the first conductive contact plate 112 respectively for transmitting current; the second conductive wire 123 is connected to the reference electrode 121 and the second conductive contact plate 122 respectively for transmitting current; and the third conductive wire 133 is connected to the counter electrode 131 and the third conductive contact plate 132 respectively for transmitting current.

[0050] It should be noted that the working electrode 111, the first conductive contact plate 112, and the first conductive wire 113 together constitute the working electrode unit 110.

[0051] It should be noted that the reference electrode 121, the second conductive contact plate 122, and the second conductive wire 123 together constitute the reference electrode unit 120.

[0052] It should be noted that the counter electrode 131, the third conductive contact plate 132, and the third conductive line 133 together constitute the counter electrode unit 130.

[0053] Furthermore, such as Figure 2 As shown, the electrode device also includes a cover plate 142 and a first sample inlet 143. The cover plate 142 is disposed opposite to the substrate 141; the first sample inlet 143 is disposed through the cover plate 142 and corresponds to the working electrode 111, for supplying urine to contact the working electrode 111.

[0054] It should be noted that the specifications of the cover plate 142 are basically the same as those of the base plate 141. The specifications here refer to the shape, length, width, etc.

[0055] It should be noted that the cover plate 142 and the substrate 141 can be fixedly connected or detachably connected, including but not limited to plugging and bonding.

[0056] It should be noted that the specifications of the first injection port 143 are matched with those of the working electrode 111. Generally, the radial dimensions (such as diameter, length, and width) of the first injection port 143 are not greater than the radial dimensions (such as diameter, length, and width) of the working electrode 111.

[0057] In some embodiments, the radial dimension (e.g., diameter, length, width) of the first injection port 143 is smaller than the radial dimension (e.g., diameter, length, width) of the working electrode 111.

[0058] Furthermore, such as Figure 2 As shown, the electrode device also includes an intermediate plate 144, a second inlet port 145, a flow channel 146, and a third inlet port 147. The intermediate plate 144 is disposed between the base plate 141 and the cover plate 142; the second inlet port 145 is disposed at least through the upper surface of the intermediate plate 144 and is opposite to the first inlet port 143, for supplying urine flow; the flow channel 146 is disposed on the intermediate plate 144 and communicates with the second inlet port 145, for supplying urine flow; the third inlet port 147 is disposed at least through the lower surface of the intermediate plate 144 and communicates with the flow channel 146, and is opposite to the working electrode 111, for supplying urine to contact the working electrode 111.

[0059] It should be noted that the specifications of the intermediate plate 144 are basically the same as those of the cover plate 142 and the base plate 141. The specifications here refer to the shape, length, width, etc.

[0060] In some embodiments, the radial dimensions (e.g., length, width) of the intermediate plate 144 are smaller than the radial dimensions (e.g., length, width) of the substrate 141 (cover plate 142).

[0061] It should be noted that the intermediate plate 144 can be fixedly connected to the base plate 141 and the cover plate 142, or it can be detachably connected, including but not limited to plugging and bonding.

[0062] It should be noted that the specifications of the second injection port 145 are matched with those of the first injection port 143. Generally, the radial dimensions (such as diameter, length, and width) of the second injection port 145 are not greater than the radial dimensions (such as diameter, length, and width) of the first injection port 143.

[0063] In some embodiments, the second injection port 145 is disposed through the upper and lower surfaces of the intermediate plate 144.

[0064] It should be noted that the specifications of the flow channel 146 are matched with those of the second injection port 145. Generally, the width of the flow channel 146 is not greater than the radial dimension (such as diameter, length, and width) of the second injection port 145, and the length of the flow channel 146 is greater than the radial dimension (such as diameter, length, and width) of the second injection port 145.

[0065] It should be noted that the flow channel 146 may not penetrate the upper or lower surface of the intermediate plate 144, or it may penetrate only the upper surface of the intermediate plate 144, or it may penetrate only the lower surface of the intermediate plate 144, or it may penetrate both the upper and lower surfaces of the intermediate plate 144.

[0066] It should be noted that the specifications of the third injection port 147 are matched with those of the flow channel 146. Generally, the radial dimension (such as diameter, length, and width) of the third injection port 147 is not less than the width of the flow channel 146, and the radial dimension (such as diameter, length, and width) of the third injection port 147 is less than the length of the flow channel 146.

[0067] It should be noted that the specifications of the third injection port 147 are matched with those of the working electrode 111. Generally, the radial dimensions (such as diameter, length, and width) of the third injection port 147 are not greater than the radial dimensions (such as diameter, length, and width) of the working electrode 111.

[0068] In some embodiments, the radial dimension (e.g., diameter, length, width) of the third inlet port 147 is smaller than the radial dimension (e.g., diameter, length, width) of the working electrode 111.

[0069] In some of these embodiments, the third injection port 147 is disposed through the upper and lower surfaces of the intermediate plate 144.

[0070] Using the electrode device described above, in conjunction with a urine oxygen content detection device, the oxygen content in urine can be detected.

[0071] It should be noted that in this invention, the detection principle is based on an electrochemical oxidation-reduction method, namely O2 + 4e. - +2H₂O=4OH - .

[0072] like Figure 3 As shown, a urine oxygen content measurement system includes an electrode device and a urine oxygen content detection device as described above. The urine oxygen content detection device is connected to the electrode device and is used to measure the urine oxygen content.

[0073] In some embodiments, the urine oxygen content detection device is a liquid oxygen content detector, which includes an electrode socket, a display screen, and control buttons. The urine oxygen content can be detected by connecting the electrode device to the electrode socket.

[0074] The technical effects of this invention are as follows: (1) Improve the utilization efficiency of precious metal Pt by using MoC support; (2) The MoC-Pt composite system demonstrated sensitivity to O2 sensing in liquids and strong anti-interference ability against common small molecules and ions in body fluids. (3) Compared with metal Pt wire or Pt sheet electrodes, the manufacturing cost can be greatly reduced.

[0075] Example 2 This embodiment is a specific implementation of the present invention.

[0076] In this embodiment, the electrode material is prepared as follows: Step 1: Weigh 300 mg of MoC powder and 9 mg of chloroplatinic acid hexahydrate into a 300 mL polytetrafluoroethylene hydrothermal reactor, and then add 250 mL of deionized water.

[0077] Step 2: Place the hydrothermal autoclave in an ultrasonic cleaner and sonicate for 1 hour to obtain a uniformly dispersed suspension. Then, place it in a water bath and keep it at 90°C with stirring for 1 hour.

[0078] Step 3: Wash with deionized water, filter, and dry to obtain MoC-9%Pt material.

[0079] The morphology of the product obtained in this embodiment is shown in the figure below. Figure 4 As shown. The resulting product consists of black particles (i.e., Pt) smaller than 10 nm, uniformly dispersed on a support (i.e., MoC).

[0080] The XRD pattern of the product obtained in this embodiment is as follows: Figure 5 As shown, a peak of metallic Pt was observed at a diffraction angle of 40°.

[0081] Example 3 This embodiment is a specific implementation of the present invention.

[0082] In this embodiment, the electrode material is prepared as follows: Step 1: Weigh 300mg of MoC powder and 3mg of chloroplatinic acid hexahydrate into a 300mL polytetrafluoroethylene hydrothermal reactor, and then add 250mL of deionized water.

[0083] Step 2: Place the hydrothermal autoclave in an ultrasonic cleaner and sonicate for 1 hour to obtain a uniformly dispersed suspension. Then, place it in a water bath and keep it at 90°C with stirring for 5 hours.

[0084] Step 3: Wash with deionized water, filter, and dry to obtain MoC-3%Pt material.

[0085] The XRD pattern of the product obtained in this embodiment is as follows: Figure 5 As shown, no peak of metallic Pt was observed at the diffraction angle of 40°.

[0086] Example 4 This embodiment is a specific implementation of the present invention.

[0087] In this embodiment, the electrode material is prepared as follows: Step 1: Weigh 300mg of MoC powder and 6mg of chloroplatinic acid hexahydrate into a 300mL polytetrafluoroethylene hydrothermal reactor, and then add 250mL of deionized water.

[0088] Step 2: Place the hydrothermal autoclave in an ultrasonic cleaner and sonicate for 1 hour to obtain a uniformly dispersed suspension. Then, place it in a water bath and keep it at 90°C with stirring for 3 hours.

[0089] Step 3: Wash with deionized water, filter, and dry to obtain MoC-6%Pt material.

[0090] The XRD pattern of the product obtained in this embodiment is as follows: Figure 5 As shown, a peak of metallic Pt can be observed at a diffraction angle of 40°.

[0091] Example 5 This embodiment is a specific implementation of the present invention.

[0092] In this embodiment, the electrode material is prepared as follows: Step 1: Weigh 300mg of MoC powder and 12mg of chloroplatinic acid hexahydrate into a 300mL polytetrafluoroethylene hydrothermal reactor, and then add 250mL of deionized water.

[0093] Step 2: Place the hydrothermal autoclave in an ultrasonic cleaner and sonicate for 1 hour to obtain a uniformly dispersed suspension. Then, place it in a water bath and keep it at 90°C with stirring for 0.5 hours.

[0094] Step 3: Wash with deionized water, filter, and dry to obtain MoC-12%Pt material.

[0095] The XRD pattern of the product obtained in this embodiment is as follows: Figure 5 As shown, a strong peak of metallic Pt can be observed at a diffraction angle of 40°.

[0096] Example 6 This embodiment is a specific implementation of the present invention.

[0097] In this embodiment, the electrode material is prepared as follows: Step 1: Weigh 300mg of MoC powder and 15mg of chloroplatinic acid hexahydrate into a 300mL polytetrafluoroethylene hydrothermal reactor, and then add 250mL of deionized water.

[0098] Step 2: Place the hydrothermal reactor in an ultrasonic cleaner and sonicate for 1 hour to obtain a uniformly dispersed suspension. Then, place it in a water bath and keep it at 70°C with stirring for 1 hour.

[0099] Step 3: Wash with deionized water, filter, and dry to obtain MoC-15%Pt material.

[0100] The XRD pattern of the product obtained in this embodiment is as follows: Figure 5 As shown, a strong peak of metallic Pt can be observed at a diffraction angle of 40°.

[0101] Test Example 1 This embodiment tests the MoC-9%Pt material prepared in Example 2.

[0102] In this embodiment, a three-electrode detection electrode is prepared for the determination of oxygen content in PBS solution. The working electrode is made of MoC-9%Pt material, the counter electrode is made of carbon electrode, and the reference electrode is made of Ag / AgCl.

[0103] The electrode cards were inserted into 0.1M PBS solution saturated with N2 and 0.1M PBS solution saturated with O2, respectively, and electrochemical tests were performed using cyclic voltammetry. The scan rate was 10 mV / s, and the potential range was -0.65 to 0.35 V (vs. Ag / AgCl).

[0104] The detection performance graph obtained in this embodiment is as follows: Figure 6 As shown, the change in electrical signal near -0.1V is very significant, indicating that the electrochemical sensor designed in this invention for detecting O2 content in liquids can significantly improve the sensing performance of O2 in liquids.

[0105] Test Example 2 This embodiment tests the MoC-9%Pt material prepared in Example 2.

[0106] In this embodiment, a three-electrode detection electrode is prepared for the determination of oxygen content in PBS solution. The working electrode is made of MoC-9%Pt material, the counter electrode is made of carbon electrode, and the reference electrode is made of Ag / AgCl.

[0107] In 15 mL of 0.1 M PBS solution saturated with N2, cyclic voltammetry was used to scan the potentials by adding 1 mL of an equal volume of 0.1 M PBS solution saturated with O2. The scan rate was 50 mV / s, and the potential range was -0.5 to 0.5 V (vs. Ag / AgCl).

[0108] The detection performance graph obtained in this embodiment is as follows: Figure 7 As shown, the peak current at -0.13V was collected and fitted, and it was found that the volume of PBS solution saturated with O2 had a linear relationship with the electrical signal intensity, indicating that the electrode has good sensing performance for the O2 content in the liquid.

[0109] Test Example 3 This embodiment tests the MoC-9%Pt material prepared in Example 2.

[0110] In this embodiment, a three-electrode detection electrode is prepared for the determination of oxygen content in PBS solution. The working electrode is made of MoC-9%Pt material, the counter electrode is made of carbon electrode, and the reference electrode is made of Ag / AgCl.

[0111] The measurement was performed using a chronoamperometry method in 15 mL of 0.1 M PBS solution saturated with N2, with the voltage set to -0.13 V (vs. Ag / AgCl). 1 mL of 0.1 M PBS solution saturated with O2 was added every 100 s.

[0112] The detection performance graph obtained in this embodiment is as follows: Figure 8 As shown, fitting the volume and current signal of a 0.1M PBS solution saturated with O2 revealed a linear relationship between the volume of the PBS solution with added O2 and the electrical signal intensity, indicating that the electrode has good sensing performance for the O2 content in the liquid.

[0113] Test Example 4 This embodiment tests the MoC-9%Pt material prepared in Example 2.

[0114] In this embodiment, a three-electrode detection electrode is prepared for the determination of oxygen content in PBS solution. The working electrode is made of MoC-9%Pt material, the counter electrode is made of carbon electrode, and the reference electrode is made of Ag / AgCl.

[0115] To 15 mL of 0.1 M PBS solution saturated with N2, 1 mL of 0.1 M PBS solution saturated with O2, 1 mM uric acid (UA), 1 mM glucose (Glu), 1 mA ascorbic acid (AA), 1 mM NaCl, 1 mM KCl, 1 mM MgCl2, and 1 mL of 0.1 M PBS solution saturated with O2 were added sequentially. Measurements were performed using chronoamperometry with the voltage set to -0.13 V (vs. Ag / AgCl).

[0116] The detection performance graph obtained in this embodiment is as follows: Figure 9 As shown, the addition of 1 mL of 0.1 M PBS solution saturated with O2 resulted in a significant change in the electrical signal, while the addition of other substances did not cause a significant change in the electrical signal, indicating that the electrode has strong anti-interference performance against some small molecules and metal ions.

[0117] Comparative Example In this comparative example, MoC without metal Pt modification was used as the electrode material for the preparation of a three-electrode detection electrode and the O2 sensing performance test.

[0118] The electrode obtained in this comparative example exhibits the following O2 sensing performance in a 0.1M PBS solution saturated with O2: Figure 10 As shown, the sensing performance of MoC without Pt modification for O2 is significantly reduced.

[0119] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0120] The embodiments described above are merely illustrative of several implementation methods of this application, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this application, and these all fall within the protection scope of this application. Therefore, the protection scope of this patent application should be determined by the appended claims.

Claims

1. An electrode material for measuring the oxygen content of urine, characterized in that, Includes MoC and Pt, with Pt loaded on the surface of MoC; Among them, MoC is a nanoscale sheet structure, and Pt is a nanoscale particle structure; The mass loading of Pt is 9%.

2. A method for preparing an electrode material for measuring urine oxygen content, used to prepare the electrode material as described in claim 1, characterized in that, include: MoC, chloroplatinic acid hexahydrate, and deionized water were ultrasonically mixed for 1 hour according to a preset ratio to obtain a uniformly dispersed suspension, wherein the mass loading of Pt was 9%. The suspension was hydrothermally heated at 90°C for 1 hour to obtain the reaction liquid; The reaction liquid is subjected to solid-liquid separation to obtain electrode materials, wherein the solid-liquid separation includes filtration, washing, and drying.

3. A working electrode for measuring the oxygen content of urine, characterized in that, It is prepared from the electrode material as described in claim 1, or from the electrode material prepared by the preparation method as described in claim 2.

4. An electrode device for measuring the oxygen content of urine, characterized in that, include: The working electrode as described in claim 3; A reference electrode, wherein the reference electrode is disposed on one side of the working electrode; The counter electrode is disposed on one side of the working electrode and the reference electrode.

5. The electrode device according to claim 4, characterized in that, Also includes: A substrate, wherein the working electrode, the reference electrode, and the counter electrode are disposed on the surface of the substrate; A first conductive contact plate is disposed on the surface of the substrate; The second conductive contact plate is disposed on the surface of the substrate; A third conductive contact plate is disposed on the surface of the substrate; The first conductive wire is connected to the working electrode and the first conductive contact plate respectively, and is used to transmit current. The second conductive wire is connected to the reference electrode and the second conductive contact plate respectively, and is used to transmit current. The third conductive wire is connected to the counter electrode and the third conductive contact plate respectively, and is used to transmit current.

6. A urine oxygen content measurement system, characterized in that, include: The electrode device as described in claim 4 or 5; A urine oxygen content detection device, which is connected to the electrode device, is used to measure urine oxygen content.

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