Compositions for enhancing skin elasticity or improving skin wrinkles

Abstract

The present disclosure relates to a composition for enhancing skin elasticity or improving skin wrinkles. The composition for enhancing skin elasticity or improving skin wrinkles of the present disclosure produces a significant synergistic effect in enhancing skin elasticity or improving skin wrinkles through the combination of adenosine triphosphate and cyanocobalamin.

Description

Technical Field

[0001] This application claims priority based on Korean Application No. 10-2023-0162485, filed November 21, 2023; Korean Application No. 10-2023-0162486, filed November 21, 2023; and Korean Application No. 10-2024-0120276, filed September 4, 2024, the entire contents disclosed in the descriptions and drawings of the respective applications are incorporated herein by reference.

[0002] This disclosure relates to a composition for enhancing skin elasticity or improving skin wrinkles. Background Technology

[0003] With an aging population and increased life expectancy, there is a growing focus on preventing skin aging by enhancing skin elasticity and reducing wrinkles. Skin, which accounts for approximately 16% of the human body's volume, exists on the outermost layer of the body, protecting us from external environmental stimuli. Compared to other internal organs, skin is constantly exposed to external stimuli and is therefore more vulnerable, especially facial skin, which is directly exposed to ultraviolet radiation and pollutants, making it more prone to aging than other parts of the body.

[0004] Skin aging is divided into naturally occurring endogenous aging and exogenous aging caused by external factors. As a major factor in endogenous aging, genetic factors are mentioned, which determine the structure and changes of skin cells and tissues. On the other hand, exogenous aging factors originate from external sources, such as nutritional deficiencies, ultraviolet radiation, and environmental pollution. Unlike genetic factors, these are completely preventable and avoidable.

[0005] Skin aging occurs due to these factors, resulting in symptoms such as dry and rough skin, wrinkles, and decreased elasticity. It is known that skin wrinkles and decreased elasticity are directly related to the reduction and deformation of the main proteins in the dermis, namely collagen and elastin. That is, skin wrinkles begin to appear with dermal aging; changes in the structure of elastic fibers, namely elastin, lead to skin laxity and wrinkles. Furthermore, numerous studies have reported that increased activity of MMPs (matrix metalloproteinases), which break down connective tissue components in the dermis, including collagen, hinders collagen synthesis, thus leading to increased wrinkles.

[0006] MMPs are metalloproteinases containing calcium-dependent zinc in their active center, secreted in vivo as potential zymogens. To acquire enzymatic activity, MMPs undergo structural changes, with the amino-terminal region being cleaved, leading to activation. Activated MMPs have their activity regulated by inhibitors such as 2-macroglobulin or TIMPs (tissue inhibitors of metalloproteinases). Most cells, including skin keratinocytes and fibroblasts, secrete MMPs. These MMPs are classified into several subfamilies based on their structural and functional characteristics. MMP1, as an interstitial collagenase, is an important protein in wound healing, tissue regeneration, and reorganization. MMP3 (Stromelysin-1) is a protease that breaks down collagen, elastin, and fibronectin. When MMP1 or MMP3 is exposed to external or internal stimuli, more is produced in the body than usual, binding to and cleaving collagen, leading to wrinkles and decreased skin elasticity.

[0007] The reality is that there is a need to discover new active ingredients that can effectively enhance skin elasticity and improve wrinkles by inhibiting the factors mentioned above that cause decreased skin elasticity or wrinkles. Summary of the Invention

[0008] Technical issues

[0009] Therefore, the problem to be solved by this disclosure is to provide a composition that has a significantly superior effect in enhancing skin elasticity or improving skin wrinkles.

[0010] Problem-solving methods

[0011] To address the aforementioned issues, the inventors of this disclosure conducted in-depth research, which resulted in the discovery that when adenosine triphosphate (ATP) and cyanocobalamin (vitamin B1) are incompatible... 12 vitamin B 12 When combined, the effects of enhancing skin elasticity or improving skin wrinkles are significantly enhanced. The inventors of this disclosure discovered that, surprisingly, when treated with adenosine triphosphate and cyanocobalamin, the expression levels of MMP1 and MMP3, collagen-degrading enzymes, were synergistically reduced, thus confirming a remarkably superior effect in enhancing skin elasticity or improving skin wrinkles, thereby completing this invention.

[0012] This disclosure provides a composition comprising adenosine triphosphate and cyanocobalamin for enhancing skin elasticity or improving skin wrinkles. The composition may contain adenosine triphosphate and cyanocobalamin as active ingredients.

[0013] In one aspect of this disclosure, the content of the aforementioned adenosine triphosphate relative to the total weight of the composition may be from 0.0001 to 20% by weight, preferably from 0.001 to 10% by weight. When the aforementioned adenosine triphosphate is less than 0.0001% by weight relative to the total weight of the composition, the effect of enhancing skin elasticity or improving skin wrinkles may be weak; when it exceeds 20% by weight, the stability of the dosage form may be hindered.

[0014] In one aspect of this disclosure, the content of the aforementioned cyanocobalamin relative to the total weight of the composition may be from 0.00001 to 1% by weight, preferably from 0.00005 to 0.01% by weight. When the content of the aforementioned cyanocobalamin is less than 0.00001% by weight relative to the total weight of the composition, the effect of enhancing skin elasticity or improving skin wrinkles may be weak; when it exceeds 1% by weight, the stability of the dosage form may be hindered.

[0015] In one aspect of this disclosure, the weight ratio of adenosine triphosphate (ATP) to cyanocobalamin can be 1:0.001 to 10, preferably 1:0.05 to 5, more preferably 1:0.01 to 1, even more preferably 1:0.02 to 0.5, and most preferably 1:0.03 to 0.1 (ATP: cyanocobalamin). When the two components are contained in the above weight ratio range, the effect of enhancing skin elasticity or improving skin wrinkles can be significantly improved.

[0016] In one aspect of this disclosure, the aforementioned enhancement of skin elasticity can mean improving skin elasticity by increasing the amount of collagen and elastin in the dermis. Collagen, a fibrous protein that makes up 90% of the dermis, provides the skin with mechanical strength, connective tissue resistance, and tissue cohesion. Elastin, a protein with very high elasticity, can form a network structure together with collagen, thereby contributing to skin elasticity.

[0017] In one aspect of this disclosure, the aforementioned improvement of skin wrinkles may mean preventing or improving skin wrinkles caused by aging or external factors by increasing the amount of collagen and elastin in the dermis.

[0018] In one aspect of this disclosure, the above-described composition can enhance skin elasticity or improve skin wrinkles by inhibiting the expression of MMP1 and MMP3 in the dermis. Specifically, the composition can increase the amount of collagen in the dermis by inhibiting MMPs that break down collagen, thereby improving the cohesion and firmness of skin tissue, thus enhancing skin elasticity and improving wrinkles.

[0019] In one aspect of this disclosure, the aforementioned composition comprising adenosine triphosphate and cyanocobalamin for enhancing skin elasticity or improving skin wrinkles can be a cosmetic composition. The aforementioned cosmetic composition may also contain conventional excipients and carriers such as antioxidants, stabilizers, solubilizers, vitamins, pigments, or fragrances. For example, the aforementioned cosmetic composition may further contain excipients such as glycerin, butylene glycol, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, citric acid, panthenol, squalane, sodium citrate, or allantoin.

[0020] In one aspect of this disclosure, since the above-described cosmetic compositions are essentially applied to the skin, they can be prepared into any commonly prepared dosage form with reference to cosmetic compositions in the art. As an example, they can be formulated as solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, oils, powder foundations, emulsion foundations, wax foundations, or sprays, but are not limited thereto. More specifically, they can be formulated as toners, nourishing lotions, nourishing creams, massage creams, serums, eye creams, sprays, or powders, etc.

[0021] In one aspect of this disclosure, when the dosage form of the above cosmetic composition is a paste, cream, or gel, the carrier component may include animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide, etc.

[0022] In one aspect of this disclosure, when the dosage form of the above cosmetic composition is a powder or a spray, the carrier component may include lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder, etc. In particular, when it is a spray, a propellant, such as chlorofluorocarbon, propane / butane or dimethyl ether, may be additionally included.

[0023] In one aspect of this disclosure, when the dosage form of the above-mentioned cosmetic composition is a solution or emulsion, the carrier component may include a solvent, a solubilizer, or an emulsifier, specifically water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic esters, polyethylene glycol, or fatty acid esters of sorbitol.

[0024] In one aspect of this disclosure, when the dosage form of the above-mentioned cosmetic composition is a suspension, the carrier component may include a liquid diluent, such as water, ethanol, propylene glycol, etc.; a suspending agent, such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitan ester, polyoxyethylene dehydrated sorbitan ester, etc.; microcrystalline cellulose, aluminum hydroxide, bentonite, agar or tragacanth gum, etc.

[0025] In one aspect of this disclosure, the aforementioned composition comprising adenosine triphosphate and cyanocobalamin for enhancing skin elasticity or improving skin wrinkles can be a health functional food composition. The aforementioned health functional food may contain food-grade food additives that are acceptable in food science, and may also contain suitable carriers, excipients, or diluents commonly used in the preparation of health functional foods.

[0026] In one aspect of this disclosure, the above-described health functional food composition can be used as a food additive, in which case the composition can be added directly or used together with other foods or food ingredients according to conventional methods. The mixing amount of the active ingredients can be appropriately determined according to the purpose of use (preventive, health, or therapeutic treatment). Examples of the types of the above-described health functional foods include dairy products including ice cream, various soups, beverages, teas, oral liquids, alcoholic beverages, and vitamin complexes. In addition to the above, the above-described health functional food composition may also contain various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, and carbonating agents used in alcoholic or carbonated beverages.

[0027] In addition, this disclosure provides a method for enhancing skin elasticity or improving skin wrinkles, comprising the step of applying the above-mentioned composition containing adenosine triphosphate and cyanocobalamin for enhancing skin elasticity or improving skin wrinkles to the skin. The same technical features as those of the above-mentioned composition for enhancing skin elasticity or improving skin wrinkles can be applied to the above method.

[0028] All ingredients described in this disclosure preferably do not exceed the maximum usage limits specified in the relevant regulations and standards of Korea, China, the United States, Europe, Japan, etc. (e.g., relevant regulations such as cosmetic safety standards (Korea), cosmetic safety technical specifications (China), Codex Alimentarius (Korea), Codex Alimentarius (Korea), Codex Alimentarius (Korea), Codex Alimentarius (Korea), etc.). That is, preferably, the cosmetic or health functional food composition of this disclosure contains the ingredients of the present invention within the content limits permitted by the relevant regulations and standards of each country.

[0029] Invention Effects

[0030] The composition disclosed herein for enhancing skin elasticity or improving skin wrinkles produces a significant synergy effect in enhancing skin elasticity or improving skin wrinkles through the combination of adenosine triphosphate and cyanocobalamin. Attached Figure Description

[0031] Figure 1 The chart shows the comparison of MMP1 or MMP3 expression levels when adenosine triphosphate and cyanocobalamin were treated alone or in combination in Experiment Example 1.

[0032] Figure 2 The graph shows a comparison of the reduction rates of MMP1 or MMP3 expression when adenosine triphosphate and cyanocobalamin were treated alone or in combination in Experimental Example 1.

[0033] Figure 3 The graph shown is a comparison of the reduction rate of MMP1 expression based on the weight ratio of adenosine triphosphate (ATP) to cyanocobalamin in Experiment Example 2. Detailed Implementation

[0034] Hereinafter, to aid in understanding the present invention, embodiments will be described in detail. However, the embodiments of the present invention can be modified in various different forms, and the scope of the present invention should not be construed as limited to the following embodiments. The embodiments of the present invention are provided to provide a more complete description of the present invention to those skilled in the art.

[0035] The adenosine triphosphate (disodium adenosine triphosphate) used in the following experimental examples was purchased and used from the manufacturer Jiangsu Huayu Chemical, China (HANJOO C&C); the cyanocobalamin was purchased and used from the manufacturer DSM, China (SeroChem).

[0036] Experiment Example 1: Synergistic effect of the combination of adenosine triphosphate (ATP) and cyanocobalamin on enhancing skin elasticity and improving skin wrinkles.

[0037] This study evaluated the effects of combined administration of adenosine triphosphate (ATP) and cyanocobalamin on skin elasticity enhancement and wrinkle improvement in skin cells. MMPs (matrix metalloproteinases) are factors influencing skin elasticity and wrinkles; this experiment evaluated the synergistic effect of regulating the expression of MMP1 and MMP3. More than 20 types of MMPs are known to be matrix proteins that break down collagen. As collagen is broken down, skin elasticity decreases, leading to wrinkles. Therefore, inhibiting MMP expression can enhance skin elasticity and improve wrinkles.

[0038] Human foreskin-derived human fibroblasts (Hs68; human fibroblast, CRL-1635) were purchased and used from ATCC. The purchased cells were cultured in DMEM (Dulbecco Modified Eagle Medium-GIBCO) medium supplemented with 10% FBS (fetal bovine serum) and 1% penicillin / streptomycin, and incubated at 37°C in a 5% CO2 incubator.

[0039] To analyze the effects of changes in MMP1 and MMP3 mRNA expression, human fibroblasts were used in 2... 10 5 Cells / well were dispensed into 6-well plates and incubated at 37°C for one day in a 5% CO2 incubator. After replacing the medium with PBS, the plates were irradiated with UVA (365nm) at 10 J / cm² using an RMX 3W (Vilber Lourmat) device. 2 After adding 10 ppm ATP and 1 ppm cyanocobalamin to the culture medium, the culture was incubated under the same conditions for one day. Total RNA was then obtained using the AccuPrep Universal RNA Extraction Kit (Bioneer, K-3140), and subsequently analyzed using AccuPower® RocketScript. TM cDNA was synthesized using Cycle (Bioneer, K-2202). The synthesized cDNA and TaqMan probes (assay ID: MMP1(Hs00899658), MMP3(Hs00968305)) were used to perform qPCR via ABI7500 real-time PCR to analyze the expression levels of the MMP1 and MMP3 genes.

[0040] Synergistic effects are determined using the Colby formula. A synergistic effect is considered to exist when the measured effect (T) of the combination is better than the predicted value (E) by the Colby formula. The Colby formula is as follows.

[0041] E = (X+Y)-(X×Y / 100)

[0042] X: Efficacy of cyanocobalamin alone relative to the control group.

[0043] Y: Efficacy of ATP alone relative to the control group.

[0044] E: Predicted efficacy of cyanocobalamin in combination with ATP relative to the control group

[0045] T: The efficacy relative to the control group when cyanocobalamin is used in combination with ATP.

[0046] from Figure 1 and Figure 2 The experimental results show that UVA irradiation increased the expression of MMP1 and MMP3, collagen-degrading enzymes, while treatment with either adenosine triphosphate (ATP) or cyanocobalamin alone resulted in a slight decrease in MMP1 and MMP3 expression. In particular, the combined treatment with ATP and cyanocobalamin significantly increased the reduction rates of both MMP1 and MMP3 expression compared to their individual treatments, indicating a synergistic effect from the combination of the two components.

[0047] Experiment Example 2: Synergistic effect of the weight ratio of adenosine triphosphate (ATP) and cyanocobalamin on skin elasticity enhancement and wrinkle improvement.

[0048] Human foreskin-derived human fibroblasts (Hs68; human fibroblast, CRL-1635) were purchased and used from ATCC. The purchased cells were cultured in DMEM (Dulbecco Modified Eagle Medium-GIBCO) medium supplemented with 10% FBS (fetal bovine serum) and 1% penicillin / streptomycin, and incubated at 37°C in a 5% CO2 incubator.

[0049] To analyze the effects of changes in MMP1 mRNA expression, human fibroblasts were used in 2... 10 5 Cells / well were dispensed into 6-well plates and incubated at 37°C for one day in a 5% CO2 incubator. After replacing the medium with PBS, the plates were irradiated with UVA (365nm) at 10 J / cm² using an RMX 3W (Vilber Lourmat) device. 2 The weight ratio of ATP to cyanocobalamin was changed to 10ppm:10ppm, 10ppm:1ppm, 10ppm:0.3ppm, or 10ppm:0.1ppm (in the order of ATP:cyanocobalamin), and added to the culture medium, and then cultured under the same conditions for one day.

[0050] Total RNA was then obtained using the AccuPrep Universal RNA Extraction Kit (Bioneer, K-3140), and then processed using AccuPower® RocketScript. TM cDNA was synthesized using Cycle (Bioneer, K-2202). The synthesized cDNA and TaqMan probe (assay ID: MMP1 (Hs00899658)) were used to perform qPCR via ABI7500 real-time PCR to analyze the expression levels of various MMP1 genes.

[0051] from Figure 3 The experimental results show that when the weight ratio of ATP to cyanocobalamin is 10 ppm:1 ppm and 10 ppm:0.3 ppm, it exceeds the Colby prediction, thus confirming a synergistic effect. When the weight ratio is 10 ppm:0.1 ppm, although it exceeds the Colby prediction, there is no significant difference compared to the combination of 0.1 ppm cyanocobalamin alone.

Claims

1. A composition for enhancing skin elasticity or improving skin wrinkles, comprising adenosine triphosphate and cyanocobalamin.

2. The composition for enhancing skin elasticity or improving skin wrinkles according to claim 1, wherein, The content of the above-mentioned adenosine triphosphate is 0.0001 to 20 by weight relative to the total weight of the composition.

3. The composition for enhancing skin elasticity or improving skin wrinkles according to claim 1, wherein, The content of the above-mentioned cyanocobalamin is 0.00001 to 1 by weight relative to the total weight of the composition.

4. The composition for enhancing skin elasticity or improving skin wrinkles according to claim 1, wherein, The weight ratio of adenosine triphosphate to cyanocobalamin is 1:0.001~10 (adenosine triphosphate: cyanocobalamin).

5. The composition for enhancing skin elasticity or improving skin wrinkles according to claim 1, wherein, The above composition inhibits the expression of MMP1 and MMP3.

6. The composition for enhancing skin elasticity or improving skin wrinkles according to claim 1, wherein, The above composition is a cosmetic composition.

7. The composition for enhancing skin elasticity or improving skin wrinkles according to claim 1, wherein, The above composition is a health functional food composition.

Images