InDel molecular marker for identifying wheat scab resistance site Fhb1 inhibiting gene and application thereof

By developing the InDel molecular marker IndNC68619 on wheat chromosome 6A, the problem of resistance instability caused by the Fhb1 repressor gene was solved, enabling precise identification of the Fhb1 repressor gene and improving the accuracy and efficiency of molecular marker-assisted breeding for wheat resistance to Fusarium head blight.

CN122235367APending Publication Date: 2026-06-19YANGZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
YANGZHOU UNIV
Filing Date
2026-05-19
Publication Date
2026-06-19

AI Technical Summary

Technical Problem

Traditional breeding methods rely solely on the Fhb1 gene while ignoring its repressor factors, leading to unstable wheat resistance to Fusarium head blight and hindering the progress of marker-assisted selection breeding.

Method used

A molecular marker InDel, IndNC68619, located on wheat chromosome 6A, was developed. The Fhb1 repressor gene was identified by PCR amplification. Genotypic selection was performed using primer pairs IndNC68619-F and IndNC68619-R to distinguish between 255 bp and 229 bp amplification bands to identify the repressor gene.

Benefits of technology

This method enables precise identification of the Fhb1 repressor gene, ensuring the effectiveness of resistance, improving the accuracy and efficiency of breeding, and solving the problem of unstable resistance.

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Abstract

This invention discloses a method for identifying wheat resistance sites to Fusarium head blight. Fhb1 This invention relates to the InDel molecular marker of a repressor gene and its application. The InDel molecular marker is located on chromosome 6A of wheat, and its nucleotide sequence is shown in SEQ ID NO.1. The primer pair used to amplify the InDel molecular marker is shown in SEQ ID NO.2-SEQ ID NO.3. This invention is the first to target... Fhb1 The development of tightly linked molecular markers for suppressor genes solves the problem of relying solely on molecular markers in traditional breeding. Fhb1 Ignoring the repressor genes themselves leads to unstable resistance expression. This marker can quickly identify the presence of repressor genes in the genetic background, thus providing a basis for... Fhb1 The effective exertion of resistance provides genetic screening assurance for wheat. Fhb1 Genotypic selection of suppressor genes aims to provide markers for molecular marker-assisted breeding of wheat resistance to Fusarium head blight.
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