Kit and detection method for detecting common pathogenic bacteria of otitis media based on multi-enzyme constant-temperature rapid nucleic acid amplification technology

By combining multi-enzyme isothermal rapid nucleic acid amplification (MIRA) technology with specific primers and probes and a dual detection mode, the problems of long detection time and insufficient specificity of pathogens causing otitis media are solved, realizing rapid and convenient triple simultaneous detection, which is suitable for primary healthcare and point-of-care testing.

CN122256541APending Publication Date: 2026-06-23CHONGQING DAZU DISTRICT PEOPLES HOSPITAL
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Patent Information

Application Number
CN202610423730.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-04-01
Publication Date
2026-06-23

AI Technical Summary

Technical Problem

Existing technologies for detecting pathogens causing otitis media are time-consuming, complex to operate, and lack specificity and resistance to interference, making it difficult to meet the rapid diagnostic needs of primary healthcare institutions and bedside testing.

Method used

Using multi-enzyme isothermal rapid nucleic acid amplification (MIRA) technology, specific primers and probes are designed and combined with dual detection modes of fluorescence and colloidal gold to achieve triple simultaneous detection of Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis. This simplifies the operation process and is suitable for ordinary isothermal equipment.

Benefits of technology

It achieves rapid, efficient, and convenient triple simultaneous detection, shortens detection time, and improves the specificity and sensitivity of the test. It is suitable for primary healthcare and point-of-care testing, and avoids the problem of drug resistance caused by indiscriminate drug use.

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Abstract

The invention provides a kit for detecting common pathogenic bacteria of otitis media based on a multi-enzyme constant-temperature rapid nucleic acid amplification technology. The kit comprises a first primer pair for detecting streptococcus pneumoniae, a second primer pair for detecting haemophilus influenzae, a third primer pair for detecting moraxella catarrhalis, a fluorescent probe combination and a colloidal gold probe combination, the nucleotide sequences of the first primer pair are respectively SEQ ID NO: 2 and SEQ ID NO: 4, the nucleotide sequences of the second primer pair are respectively SEQ ID NO: 9 and SEQ ID NO: 10, and the nucleotide sequences of the third primer pair are respectively SEQ ID NO: 13 and SEQ ID NO: 18. According to the triple multienzyme constant-temperature rapid synchronous detection kit and the detection method, high sensitivity, high specificity and operation convenience are achieved, and the clinical bedside rapid diagnosis requirement is met.
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