Methods and systems for cell culture

By using pressurized air or gas transfer through the venting tubes of transfer tubes and immersion tubes in the bioreactor, combined with gamma-ray irradiation sterilization, the problem of aseptic transfer of dry materials was solved, achieving aseptic, flexible, and accurate transfer, reducing the risk of contamination, and improving cell culture efficiency.

CN122278618APending Publication Date: 2026-06-26CYTIVA SWEDEN AB
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CYTIVA SWEDEN AB
Filing Date
2016-05-16
Publication Date
2026-06-26

AI Technical Summary

Technical Problem

Existing technologies struggle to aseptically transfer dry materials, such as microcarriers, into bioreactors without compromising sterility, especially in large, single-use bioreactors, where conventional methods pose risks of incomplete transfer and contamination.

Method used

The system utilizes a transfer tube connected to a container of dried material and a bioreactor, with pressurized air or gas supplied via the venting tube of the immersion tube for aseptic transfer. This ensures that the dried material does not rotate within the container, and the transfer volume is controlled by weighing. The system is sterilized using gamma-ray irradiation.

Benefits of technology

This technology enables the aseptic, flexible, and accurate transfer of dried materials in large bioreactors, reducing the risk of contamination and improving the efficiency and safety of cell culture.

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Abstract

This invention relates to a method and system for transferring sterile materials from a storage container to a bioreactor. More specifically, this invention relates to a method and system for aseptically transferring dried materials (such as microcarriers) for cell culture to a bioreactor, comprising transferring the microcarriers from a first container to the bioreactor by connecting a first container containing the microcarriers to a transfer tube of the bioreactor for cell culture, wherein the transfer is accomplished using pressurized air or gas supplied to the container.
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