A method for determining the fingerprint of a dry ginger and medlar seed mixed plant solid beverage

Abstract

This invention provides a method for constructing or detecting the fingerprint spectrum of a mixed plant-based solid beverage containing dried ginger and wolfberry. The medicinal materials used in the mixed plant-based solid beverage are dried ginger, cinnamon, wolfberry, raspberry, jujube, licorice, yam, and polygonatum. The method includes the preparation of a reference solution and a test solution, and the determination of the reference solution and test solution by high-performance liquid chromatography (HPLC). The chromatographic conditions used are: a C18 column, with 0.1% phosphoric acid aqueous solution as mobile phase A and acetonitrile as mobile phase B, using gradient elution.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine quality analysis, and particularly relates to a method for determining the high performance liquid chromatography (HPLC) fingerprint of a dry ginger and wolfberry mixed plant solid beverage. Background Technique

[0002] The dry ginger and wolfberry mixed plant solid beverage is commercially named "Jiangguiyin", and is composed of eight traditional Chinese medicines, namely dry ginger, cinnamon, wolfberry, raspberry, Chinese date, licorice, Chinese yam, and polygonatum. It has the effects of warming the middle-jiao to dispel cold and warming and tonifying the spleen and kidney. Dry ginger is the dried rhizome of the ginger plant of the Zingiberaceae family, pungent and hot. It belongs to the spleen, stomach, kidney, heart, and lung meridians, and has the effects of warming the middle-jiao to dispel cold, restoring yang and unblocking meridians, and warming the lungs to resolve fluid-retention. It can be used for cold pain in the epigastrium and abdomen, vomiting and diarrhea, cold limbs with weak pulse, and cold fluid-retention cough. Cinnamon is the dried bark of the cinnamon plant of the Lauraceae family, pungent, sweet, and very hot. It belongs to the kidney, spleen, heart, and liver meridians, and has the effects of tonifying fire and assisting yang, guiding floating yang back to the origin, dispelling cold and relieving pain, and warming and unblocking meridians. It can be used for impotence and cold uterus, cold pain in the waist and knees, asthma due to kidney deficiency, floating yang due to deficiency, dizziness and red eyes, cold pain in the heart and abdomen, deficiency-cold vomiting and diarrhea, cold hernia pain, dysmenorrhea and amenorrhea. Wolfberry is the dried ripe fruit of the wolfberry plant of the Solanaceae family, sweet and flat. It belongs to the liver and kidney meridians, and has the effects of nourishing the liver and kidney and improving eyesight. It can be used for consumptive fatigue with spermatorrhea, soreness in the waist and knees, etc. Chinese yam is sweet and neutral in nature. It belongs to the spleen, lung, and kidney meridians, and has the effects of strengthening the spleen, tonifying the lung, consolidating the kidney, and benefiting essence. It is used for less food intake due to spleen deficiency and chronic diarrhea. Stir-fried Chinese yam with bran strengthens the spleen and stomach. It is used for less food intake due to spleen deficiency, loose stools, and excessive leucorrhea. Raspberry is the dried fruit of the raspberry plant of the Rosaceae family, sweet, sour, and warm. It belongs to the liver, kidney, and bladder meridians. It benefits the kidney and consolidates essence, reduces urination, and nourishes the liver and improves eyesight. It is used for spermatorrhea and emission, enuresis and frequent urination, impotence and premature ejaculation, and dim vision. Chinese date is sweet and warm. It belongs to the spleen, stomach, and heart meridians. It replenishes qi in the middle-jiao and nourishes blood and soothes the nerves. It is used for less food intake due to spleen deficiency, fatigue and loose stools, and hysteria of women. Polygonatum is the dried rhizome of Polygonatum kingianum Coll. et Hemsl., Polygonatum sibiricum Redoute, and Polygonatum cyrtonema Hua of the Liliaceae family. It is bitter, pungent, and warm in nature. It belongs to the spleen, lung, and kidney meridians. It supplements qi and nourishes yin, strengthens the spleen, moistens the lungs, and benefits the kidneys. It is used for qi deficiency of the spleen and stomach, physical fatigue, insufficient stomach yin, dry mouth and less food intake, dry cough due to lung deficiency, cough with hemoptysis due to consumptive disease, insufficient essence and blood, soreness in the waist and knees, premature whitening of hair, and internal heat and thirst. Licorice is sweet and neutral in nature. It belongs to the heart, lung, spleen, and stomach meridians. It invigorates the spleen and replenishes qi, clears heat and detoxifies, dispels phlegm and relieves cough, alleviates spasm and pain, and harmonizes various medicines. It is used for weakness of the spleen and stomach, fatigue and weakness, palpitation and shortness of breath, cough with profuse phlegm, spasm and pain in the epigastrium, abdomen, and extremities, carbuncles and sores, and relieving the toxicity and potency of drugs.

[0003] Ginger and Cinnamon Decoction has a diverse and complex composition, and current quality testing methods for this product are relatively limited, with a comprehensive quality control system yet to be established. For this type of traditional Chinese medicine composition, its efficacy stems from the synergistic effect of multiple medicinal materials, rather than the independent effect of a single component. Therefore, changes in the content and ratio of active substances in each raw material significantly affect the overall efficacy. Considering the complexity of traditional Chinese medicine compound components, it is difficult to comprehensively assess its quality based solely on individual active or indicator components. Identification methods that fully reflect the characteristics of multiple components are necessary for effective quality control of traditional Chinese medicine compositions and their preparations.

[0004] Traditional Chinese medicine (TCM) fingerprinting is a comprehensive and quantifiable analytical technique based on systematic research of the chemical components of TCM. It is widely used in the quality evaluation of raw TCM materials and their semi-finished preparations, aiming to comprehensively reflect the authenticity, quality, and consistency of samples. This technique, on the one hand, effectively identifies the authenticity of TCM materials through the composition of characteristic peaks in the fingerprint spectrum; on the other hand, it can precisely control the stability of TCM quality based on the peak areas and relative proportions of the main characteristic peaks. With the help of fingerprinting, the types and contents of chemical components contained in TCM and its preparations can be characterized relatively completely, thereby enabling a holistic assessment of drug quality.

[0005] Currently, the fingerprinting technology for traditional Chinese medicine has developed into a variety of analytical methods, covering chromatographic techniques such as thin-layer chromatography (TLCS), high-performance liquid chromatography (HPLC), gas chromatography (GC), and high-performance capillary electrophoresis (HPCE), as well as spectroscopic analysis methods such as ultraviolet spectroscopy (UV), infrared spectroscopy (IR), mass spectrometry (MS), nuclear magnetic resonance (NMR), and X-ray diffraction. Summary of the Invention

[0006] This invention provides a method for constructing or detecting the fingerprint spectrum of a mixed plant solid beverage of dried ginger and wolfberry. The medicinal materials of the mixed plant solid beverage of dried ginger and wolfberry are dried ginger, cinnamon, wolfberry, raspberry, jujube, licorice, yam and polygonatum.

[0007] The method for establishing the fingerprint spectrum of the mixed plant solid beverage of dried ginger and wolfberry of the present invention includes the following steps:

[0008] 1) Prepare the reference solution;

[0009] 2) Prepare the test solution;

[0010] 3) Take the reference solution and the test solution for high-performance liquid chromatography (HPLC) detection;

[0011] 4) Prepare fingerprint patterns.

[0012] This method is accurate, reliable, and simple to operate, providing a basis for the quality control of this solid beverage.

[0013] The fingerprint chromatogram detection method for a mixed plant solid beverage of dried ginger and wolfberry of this invention includes: taking the test sample solution for high performance liquid chromatography (HPLC) detection to obtain the fingerprint chromatogram. The chromatographic conditions used are: C18 column, 0.1% phosphoric acid aqueous solution as mobile phase A, acetonitrile as mobile phase B, and gradient elution. The gradient elution program is as follows:

[0014]

[0015] To improve the accuracy of the fingerprint chromatogram, the reference solution can be tested under the same liquid chromatography conditions before and after the test sample, and the chromatographic peaks can be identified through the chromatogram of the reference solution. The selection of the reference standard was based on the inventor's previous UPLC-Q-Exactive-Orbitrap-MS detection results on the mixed ginger and wolfberry plant solid beverage, followed by literature review, screening of indicator components of each herb in the mixed ginger and wolfberry plant solid beverage, and experimental testing. Finally, the ellagic acid peak, which showed good peak shape, no interference from impurity peaks before and after the test sample solution of the mixed ginger and wolfberry plant solid beverage, and complete separation, was selected as the reference peak for the fingerprint chromatogram.

[0016] The preparation method of the reference solution is as follows: dissolve the reference standard ellagic acid in a solvent that can dissolve ellagic acid. In this invention, methanol and / or ethanol are preferred, with methanol being the most preferred. There are no special requirements for the concentration; any concentration can be used as long as it meets the upper and lower limit detection requirements of the instrument. The concentrations given in the embodiments of this invention are 18 μg / mL to 22 μg / mL, specifically 20 μg / mL.

[0017] The preparation process of the test solution involves extracting a mixed plant solid beverage of dried ginger and wolfberry. The method is as follows: Take the mixed plant solid beverage of dried ginger and wolfberry and add a solvent for extraction. The solvent can be a solvent commonly used in the field for the extraction of natural compounds, including but not limited to alcohols or aqueous solutions of alcohols, such as methanol or ethanol. In some embodiments of the present invention, methanol or a 60%-80% aqueous solution of ethanol is used, specifically methanol. The extraction method can use conventional natural compound extraction methods in the field, such as ultrasonic extraction or reflux extraction. In this embodiment of the present invention, ultrasonic extraction was specifically used, and methanol was used for ultrasonic extraction for 1 hour. Comparison of several different extraction methods revealed that the chromatogram obtained by ultrasonic extraction had a more stable baseline, higher chromatographic peaks, and better peak shapes, making it the optimal method.

[0018] After extraction, the extract is filtered, the filtrate is evaporated to dryness, and the residue is dissolved in a reconstitution solvent. The filtrate is then filtered again, or the supernatant is collected after centrifugation. The reconstitution solvent is preferably the same as the extraction solvent. The specific concentration of the reconstituted solution does not need to be strictly within a certain range; it only needs to be sufficient for the equipment to detect the components. A suitable range can be selected as: weight of the mixed ginger and goji berry plant-based solid beverage: volume of reconstitution solvent = (0.5~1.5) g: 1 mL, for example, 0.5 g, 1 g, or 1.5 g of the mixed ginger and goji berry plant-based solid beverage / mL of reconstitution solvent.

[0019] Under the above conditions, the fingerprint spectrum of the mixed ginger and wolfberry plant solid beverage has 10 common characteristic peaks, of which peak 9 is ellagic acid. When the fingerprint spectrum is taken as reference (S) with the chromatographic peak of ellagic acid at peak 9, the relative retention times of the remaining 9 common characteristic peaks are as follows:

[0020]

[0021] Generally, after identifying the characteristic peaks in a traditional Chinese medicine product using fingerprint chromatography, the presence of the characteristic peaks at their designated positions is sufficient. However, when higher requirements are placed on the fingerprint chromatography, not only must the peak positions meet the requirements, but the peak areas must also meet certain criteria to indicate that the content of each component in the composition meets specific requirements. When the fingerprint chromatogram uses the chromatographic peak of ellagic acid (peak 9) as a reference (S), the relative peak areas of the common characteristic peaks are:

[0022]

[0023] In preparing the fingerprint chromatogram, to achieve greater accuracy, chromatographic peaks can be identified using the chromatogram of a reference standard. Fingerprint chromatograms of the mixed ginger and wolfberry plant-based solid beverage are prepared using chromatograms from different batches, preferably at least 5 batches, and more preferably at least 10 batches. The fingerprint chromatogram of the tested mixed ginger and wolfberry plant-based solid beverage is compared with the fingerprint chromatogram constructed according to this invention. A relative retention time within ±5% of the specified value indicates a qualified product. A similarity evaluation is performed between the fingerprint chromatogram and the tested product; a similarity greater than 0.8, preferably greater than 0.90, indicates a qualified product.

[0024] The fingerprint chromatogram establishment method of this invention uses a common C18 column. For more optimized conditions, a 4.6 mm × 150 mm, 3-5 μm column is used. According to the high-performance liquid chromatography (HPLC) detection of this invention, the C18 column can be an InertSustain® AQ-C18 or an XBridge® C18 column. In one embodiment of this invention, an XBridge® C18 column (4.6 mm × 150 mm, 5 μm) is used. The theoretical plate number, calculated based on ellagic acid, should be no less than 5000. This invention has found that the chromatograms obtained using an XBridge® C18 column (4.6 mm × 150 mm, 5 μm) exhibit better peak shapes and the best resolution. Therefore, it can be considered a preferred column for subsequent studies.

[0025] The wavelength range of 250-260 nm can achieve peak elution of various components, which can meet the requirements for fingerprint spectrum preparation. Taking the reference standard of this invention as an example, ellagic acid has the best absorption effect at 254 nm.

[0026] This invention does not involve temperature-sensitive substances or operations, therefore there are no special limitations on column temperature, and normal detection can usually be achieved under laboratory conditions. Therefore, a high-performance liquid chromatography (HPLC) detection environment of 20–40 °C is recommended; for example, 30 °C or 25 °C can be set in the embodiments of this invention.

[0027] The flow rate of the mobile phase can be adjusted according to equipment performance and operating efficiency, provided that the peaks of each component are well separated. Excessive flow rate leads to high equipment wear and tear, while insufficient flow rate results in prolonged processing time. In this embodiment of the invention, based on the instrument and equipment specifications and operating efficiency, the most commonly used flow rate of 1 ml / min is used. -1 and at a flow rate of 0.8-1.3 ml·min. -1 The range of tests can be adjusted.

[0028] There is generally no fixed limit to the injection volume in high-performance liquid chromatography (HPLC), as long as it meets the requirements of ease of operation and the basic detection limit of the equipment. The injection volume can be 5.0-20.0 μL, as is the standard for HPLC methods, such as 5 μL or 10 μL.

[0029] In this invention, "optional," "optionally," or "optionally exist" means that the event or situation described below may but not necessarily occur, and the description includes both cases where the event or situation occurs and cases where it does not occur.

[0030] In this invention, "comprising" and "including" are both open-ended expressions, meaning they include the content specified in this invention, but do not exclude other aspects. It should be understood that "comprising" and "including" can also encompass a closed meaning, that is, "consisting of".

[0031] The beneficial effects of this invention:

[0032] Traditional Chinese medicine fingerprinting is mainly used to evaluate the stability and reproducibility of the quality of Chinese medicinal materials and their preparations. This invention uses chemical fingerprinting as a quality control method to rapidly and intuitively identify the pharmacodynamic material basis of a mixed ginger and wolfberry plant solid beverage, identifying 10 common peaks, thus laying the foundation for establishing comprehensive quality standards for the mixed ginger and wolfberry plant solid beverage.

[0033] By analyzing 10 batches of mixed ginger and wolfberry plant-based solid beverages, an HPLC fingerprinting method for determining the fingerprint of mixed ginger and wolfberry plant-based solid beverages was established, and the quality was comprehensively evaluated. Precision, repeatability, and stability experiments verified that the method of this invention has high stability and reliability, and therefore can be used as a method for determining the fingerprint of mixed ginger and wolfberry plant-based solid beverages. Attached Figure Description

[0034] Figure 1A This is the chromatogram of ellagic acid reference standard;

[0035] Figure 1B Fingerprint spectrum of a mixed plant-based solid beverage containing dried ginger and wolfberry;

[0036] Figure 1C This is a superimposed HPLC fingerprint of 10 batches of mixed plant solid beverage containing dried ginger and wolfberry.

[0037] Figure 2A Fingerprint spectroscopy of a mixed plant solid beverage containing dried ginger and wolfberry under elution conditions;

[0038] Figure 2B Fingerprint spectrum of a mixed plant solid beverage of dried ginger and wolfberry under elution condition two. Detailed Implementation

[0039] The technical solution of the present invention will be further described in detail below with reference to specific embodiments. It should be understood that the following embodiments are merely illustrative and explanatory of the present invention, and should not be construed as limiting the scope of protection of the present invention. All technologies implemented based on the above content of the present invention are covered within the scope of protection intended by the present invention.

[0040] Unless otherwise stated, the raw materials and reagents used in the following examples are commercially available products or can be prepared by known methods.

[0041] Experimental instruments and reagents:

[0042] 1. Instruments

[0043] High-performance liquid chromatograph (model: LC-20A, Shimadzu Corporation, Japan); magnetically stirred water bath (model: LC-WB-8+, Shanghai Lichen Bangxi Instrument Technology Co., Ltd.); XS105 electronic balance (Sartorius Scientific Instruments (Beijing) Co., Ltd.); Milli-Q ultrapure water purification system (Beijing Wuzhou Dongfang Technology Co., Ltd.).

[0044] 2. Reagents

[0045] Ellagic acid (purity ≥98%, CAS: 476-66-4, China National Institutes for Food and Drug Control); 10 batches (S1-S10) of mixed plant-based solid beverage of dried ginger and wolfberry, batch numbers 202101171, 202101181, 202101301, 202104181, 202105131, 202110151, 202111131, 202112271, 202202251, 202204151, source: Shanxi Yabao Jiuhe Pharmaceutical Technology Co., Ltd.; chromatographic grade acetonitrile; chromatographic grade methanol; ultrapure water.

[0046] Example 1: Establishment of a standard fingerprint spectrum for a mixed plant-based solid beverage containing dried ginger and wolfberry.

[0047] Fingerprint patterns were prepared under the following conditions; see [link to results]. Figure 1A , Figure 1B and Figure 1C .

[0048] 1. Chromatographic conditions

[0049] HPLC fingerprint analysis conditions were performed using XBridge. ® C18 column (4.6 mm × 150 mm, 5 μm); mobile phase A (0.1% aqueous phosphoric acid solution), mobile phase B (acetonitrile); gradient elution; flow rate 1.0 mL / min. -1 Column temperature 30℃; injection volume 5μL; detection wavelength 254 nm.

[0050] Table 1 Gradient elution method

[0051]

[0052] 2. Preparation of reference solution

[0053] Accurately weigh ellagic acid reference standard and add methanol to prepare a reference solution with a concentration of 20.0 μg / mL.

[0054] 3. Preparation of test solution

[0055] Weigh 5.0g of the mixed ginger and wolfberry plant solid beverage granules and place them in a stoppered conical flask. Add an appropriate amount of methanol, extract by sonication for 1 hour, shake well, filter, and evaporate the filtrate to dryness. Redissolve the residue in 5mL of methanol, centrifuge twice at 12,000 r / min for 10 minutes each time, and collect the supernatant.

[0056] 4. HPLC fingerprint analysis of a mixed plant-based solid beverage containing dried ginger and wolfberry

[0057] 4.1 Precision Test

[0058] A batch (batch number: 202101181) of a mixed plant-based solid beverage of dried ginger and wolfberry was used as the test sample. Six consecutive injections were performed, and the retention time and peak area of ​​each common chromatographic peak were recorded. Using the retention time and peak area of ​​ellagic acid (peak 9) as a reference, the relative retention time and relative peak area of ​​each common peak were calculated to examine the consistency of the ratios of relative retention time and relative peak area. The results showed that the RSD of the relative retention time of each common peak was less than 1%, and the RSD of the relative peak area was less than 3%, indicating that the determination method of this invention is stable, has good system tightness, and is stable and reliable.

[0059] 4.2 Stability Test

[0060] A batch (batch number: 202101181) of a mixed plant-based solid beverage of dried ginger and wolfberry was tested and analyzed at different time points (0 h, 2 h, 4 h, 6 h, 8 h, 12 h, and 24 h). The retention time and peak area of ​​each common chromatographic peak were recorded. Using the retention time and peak area of ​​ellagic acid (peak 9) as a reference, the relative retention time and relative peak area of ​​each common peak were calculated to examine the consistency of the ratio of relative retention time to relative peak area. The results showed that the RSD of the relative retention time of each common peak was less than 1%, and the RSD of the relative peak area was less than 3%. This indicates that the mixed plant-based solid beverage of dried ginger and wolfberry of the present invention has good stability under experimental conditions and can guarantee the reliability of analytical results within 24 hours.

[0061] 4.3 Repeatability Test

[0062] Six parallel solutions of a mixed plant-based solid beverage of dried ginger and wolfberry from the same batch (batch number: 202101181) were prepared according to the method described in section "3". These solutions were injected and analyzed separately, and the retention time and peak area of ​​each common chromatographic peak were recorded. Using the retention time and peak area of ​​ellagic acid (peak 9) as a reference, the relative retention time and relative peak area of ​​each common peak were calculated to examine the consistency of the ratios of relative retention time and relative peak area. The results showed that the RSD of the relative retention time of each common peak was less than 1%, and the RSD of the relative peak area was less than 2.8%, indicating that the method of this invention is stable, reliable, and suitable for widespread application.

[0063] 4.4 Establishment and Similarity Evaluation of HPLC Fingerprint for Mixed Plant Solid Beverage of Dried Ginger and Goji Berries

[0064] Ten batches of mixed ginger and wolfberry plant-based solid beverages (batch numbers: 202101171, 202101181, 202101301, 202104181, 202105131, 202110151, 202111131, 202112271, 202202251, 202204151) were prepared according to the method described in section “3”, and the samples were injected for analysis. Chromatograms were recorded for each batch. A representative chromatogram (mixed ginger and wolfberry plant-based solid beverage batch number 202101181) was selected as the reference fingerprint chromatogram. The “Similarity Evaluation System for Chromatogram Fingerprints of Traditional Chinese Medicine” recommended by the Pharmacopoeia Commission was used for analysis. Chromatogram similarity was used as the fingerprint chromatogram evaluation index, and a common pattern for the fingerprint chromatogram was generated by multi-point calibration full-spectrum matching. The similarity between each batch of samples and the control fingerprint chromatograms were (1.000, 0.999, 0.999, 1.000, 0.997, 0.999, 0.999, 1.000, 0.999, 0.999).

[0065] 4.5 Identification of common peaks in fingerprint spectra, calculation of relative peak area and relative retention time

[0066] Using a similarity evaluation system, 10 peaks were detected in 10 batches of fingerprint chromatograms. Compared with the reference standard, peak number 9 was identified as ellagic acid, with a retention time of 19.061 min. Peak number 9 had a good peak shape, with no interference from impurity peaks before or after it, indicating complete separation; therefore, it was selected as the reference peak for calculating the relative peak area and relative retention time of other common peaks. The results are shown in Tables 2 and 3 below.

[0067] Table 2. Relative peak areas of the 10 common peaks in 10 batches of samples (S1~S10)

[0068]

[0069] Table 3. Relative retention times of 10 common peaks in 10 batches of samples (S1~S10)

[0070]

[0071] Example 2: Comparison of methods for preparing fingerprint spectroscopy of a mixed plant-based solid beverage containing dried ginger and wolfberry.

[0072] The determination of chromatographic conditions and the exploration of fingerprinting methods were investigated, and the fingerprinting results of ginger and cinnamon drink were prepared under different high-performance liquid chromatography conditions.

[0073] Washing conditions

[0074] The inventors compared several different elution conditions and proved that the mobile phase elution conditions currently selected in this invention are optimal. Due to the large number of samples, only a brief example is given below: Granules of this product were taken and prepared according to the method for preparing the test solution in Example 1. The following two elution conditions were compared, and the results are shown in [Figure 1]. Figure 2A and Figure 2B It can be seen that under elution condition one, the peaks of each component are well-defined and have good separation. However, under elution condition two, the reference peak and the small peak on the left are not completely separated, and the peak shape of peak 6 is poor. Therefore, elution condition one is selected as the elution condition for the fingerprint chromatogram of the mixed ginger and wolfberry plant solid beverage sample.

[0075] Washing condition one:

[0076]

[0077] Washing condition two:

[0078]

[0079] The embodiments of the present invention have been described above. However, the present invention is not limited to the above embodiments. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A fingerprint spectrum detection method for a traditional Chinese medicine composition of dried ginger and wolfberry mixed plant solid beverage, wherein the raw materials of the traditional Chinese medicine composition are dried ginger, cinnamon, wolfberry, raspberry, jujube, licorice, yam, and polygonatum, and the detection method includes: The test solution was subjected to high-performance liquid chromatography (HPLC) to obtain a fingerprint chromatogram. The HPLC conditions were as follows: C18 column, 0.1% phosphoric acid aqueous solution as mobile phase A, acetonitrile as mobile phase B, and gradient elution. The gradient elution program was as follows: The detection wavelength is 250-260 nm; The preparation method of the test solution is as follows: take a mixed plant solid beverage of dried ginger and wolfberry, add an extraction solvent for extraction, filter the extract after extraction, evaporate the filtrate to dryness, add a redissolving solvent to dissolve the residue, filter and take the filtrate or take the supernatant after centrifugation; the extraction solvent and redissolving solvent are the same and are selected from methanol and 60%-80% ethanol aqueous solution.

2. The method as described in claim 1, characterized in that, It also includes the detection of a reference solution under the same high-performance liquid chromatography conditions, wherein the reference is ellagic acid, and the reference solution is prepared by taking the reference ellagic acid, dissolving it in methanol or ethanol, and the concentration of ellagic acid in the reference solution is 18 μg / mL to 22 μg / mL.

3. The method as described in claim 1, characterized in that, The fingerprint spectrum has 10 characteristic peaks, of which peak 9 is ellagic acid. Using the chromatographic peak of ellagic acid (peak 9) as a reference (S), the relative retention times of the 10 characteristic peaks are: 。 4. The method as described in claim 3, characterized in that, When the fingerprint spectrum uses the chromatographic peak of ellagic acid (peak 9) as a reference (S), the relative peak areas of the 10 characteristic peaks are: 。 5. The method according to any one of claims 1-4, characterized in that, The detection wavelength of the high-performance liquid chromatography is 254 nm.

6. The use of the method according to any one of claims 1-5 in the quality control of a mixed plant solid beverage of dried ginger and wolfberry: the fingerprint spectrum of the mixed plant solid beverage of dried ginger and wolfberry to be tested is compared with the fingerprint spectrum of the standard mixed plant solid beverage of dried ginger and wolfberry. The product is qualified if the relative retention time is within ±5% of the specified value. The product is qualified if the similarity is evaluated with the standard fingerprint spectrum and the similarity is greater than 0.8.

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