A method to promote vigorous seedling growth of Liangwang tea
By treating Liangwang tea seeds with river sand and cooked barley flour, combined with birch fermented material and multi-element nutrient solution, the problem of low seedling efficiency of Liangwang tea was solved, and early emergence and strong seedlings were rapidly improved.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- YUNNAN ACAD OF FORESTRY
- Filing Date
- 2026-06-01
- Publication Date
- 2026-06-30
Smart Images
Figure CN122296192A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of artificial cultivation technology of Liangwang tea, specifically to a method for promoting the growth and vigor of Liangwang tea seedlings. Background Technology
[0002] Liangwang tea is a unique plant with both medicinal and edible properties. It has a sweet and slightly bitter taste, and is cool in nature. The entire plant is non-toxic and can be used medicinally, possessing the effects of clearing heat and detoxifying, promoting blood circulation and relaxing muscles. The root bark and stem bark can clear heat and detoxify, reduce inflammation and relieve coughs, and strengthen the stomach and aid digestion. It is commonly used in folk medicine to treat colds, coughs, pharyngitis, indigestion, diarrhea, ascariasis, injuries, irregular menstruation, and rheumatic bone pain. Furthermore, the tender shoots of Liangwang tea can be eaten raw in salads or stir-fried as a vegetable. They are not only delicious but also rich in protein, dietary fiber, and anthocyanins, and low in fat, making them an ecologically healthy forest vegetable. The older leaves can be processed into tea, demonstrating high development value.
[0003] With the increasing activity of humans and the development of various health benefits of Liangwang tea, wild resources of Liangwang tea are dwindling, leading to its cultivation. Currently, artificial cultivation of Liangwang tea mainly involves sowing and cutting. Cuttings require branches, which are difficult to obtain. Sowing, on the other hand, only requires seeds and is relatively easy to manage. However, Liangwang tea seeds generally require sand stratification before sowing, and germination typically takes about two months, resulting in low overall sowing efficiency. Furthermore, seedlings are typically considered robust when they reach a height of 25cm and a stem diameter of 2-4mm, but it usually takes about three months to achieve a certain level of robustness after sowing. Therefore, considering the characteristics of Liangwang tea, improving its seedling efficiency is an important research direction at present. Summary of the Invention
[0004] To address the shortcomings of existing technologies, this invention provides a method for promoting robust seedling growth in Liangwang tea, effectively accelerating seedling emergence and further enhancing root growth, thereby achieving a high rate of robust seedlings in a shorter time.
[0005] To achieve the above objectives, the present invention provides the following technical solution: A method for promoting vigorous seedling growth of Liangwang tea, the method comprising the following steps: (1) Seed pretreatment: Mix river sand and cooked barley flour in a mass ratio of 6-10:1 to obtain compound material. Bury the defatted Liangwang tea seeds in the compound material for 10-15 days. During the burial period, control the humidity at 10%-20%. Then take them out and clean them to obtain pretreated seeds. (2) Prepare seedling substrate according to the following weight parts: 15-25 parts garden soil, 10-12 parts birch fermented material, and 4-6 parts manure; wherein the birch fermented material is prepared by soaking birch dead branches in a solution with a pH of 3.0-3.5, then fermenting them with yeast, then boiling and sterilizing them, and then drying them. (3) Prepare the nutrient solution according to the following ingredients: calcium nitrate 0.9-1.0 g / L, potassium nitrate 0.6-0.8 g / L, potassium dihydrogen phosphate 0.3-0.4 g / L, magnesium sulfate 0.4-0.5 g / L, boric acid 3-5 mg / L, manganese sulfate 1-2 mg / L, disodium ethylenediaminetetraacetate 10-20 mg / L, soybean seed polysaccharide 4-8 mg / L, and add water to make up to 1 L; (4) Sowing: After disinfecting the pretreated seeds, sow them into the seedling substrate, cover them with a layer of pine needles, and water them thoroughly with nutrient solution. After the seedlings emerge, remove the pine needles and water them with nutrient solution again.
[0006] Preferably, the method for defatting Liangwang tea seeds in step (1) is to place the seeds in a sodium carbonate solution with a mass concentration of 0.8%-0.15% and rub them repeatedly until the oil on the seed surface is completely washed off, and then rinse them with clean water.
[0007] Preferably, the cooked barley flour is made by roasting hulled barley at 140-180℃ for 8-10 minutes and then grinding it into powder and passing it through a 10-mesh sieve.
[0008] Preferably, the specific preparation method of the birch fermented material includes the following steps: S1. Cut birch dead branches into sections and soak them in a citric acid solution with a pH of 3.0-3.5 for 20-30 minutes. Then rinse with clean water until the pH is neutral to obtain pretreated birch branches. S2. Grind the pretreated birch branches into a slurry with 2-3 times the volume of water, then inoculate with 0.1%-0.2% of the total mass of the pretreated birch branches with yeast, and ferment for 48-60 hours to obtain fermented material for later use. S3. Boil the fermented material until it boils, then keep it warm for 8-10 minutes for sterilization, and then dry it to obtain birch fermented material.
[0009] Preferably, the length of the birch twig cuttings in step S1 is 1-3 cm.
[0010] Preferably, the manure in step (2) is prepared by composting fresh animal manure and straw at a mass ratio of 3-5:1 for 90-120 days.
[0011] Preferably, the pretreatment of seeds in step (4) involves soaking the pretreated seeds in a 500-fold dilution of carbendazim for 10-15 minutes.
[0012] Preferably, in step (4), water is applied promptly before and after seed germination to ensure that the moisture content of the seedling substrate is between 50% and 70%.
[0013] Preferably, in step (4), after the pretreated seeds are sown, they are covered with a 2-3cm layer of seedling substrate, and a 0.5-1cm thick layer of pine needles is placed on top of the seedling substrate.
[0014] This invention provides a method for promoting the growth and vigorous development of Liangwang tea seedlings, which has the following advantages compared with the prior art: This invention uses cooked barley flour and river sand to treat defatted Liangwang tea seeds, which can effectively promote early germination and seedling emergence, shorten the emergence time after sowing. The addition of birch fermented material to the substrate further promotes root growth in Liangwang tea seedlings, effectively increasing the number of lateral roots and improving nutrient absorption, thus ensuring a higher survival rate after transplanting. The multi-element compound nutrient solution effectively promotes seedling growth, and the addition of tamarind seed polysaccharides further promotes early emergence and increases stem thickness, comprehensively improving the robust seedling effect of Liangwang tea cultivation. Attached Figure Description
[0015] Figure 1 This is a comparison chart showing the growth of Liangwang Tea seedlings in seedling bags 68 days after sowing in various experimental groups in this embodiment of the invention. Figure 2 This is a schematic diagram showing the overall growth of Liangwang tea seedlings 68 days after sowing in experimental group 1 of this invention. Figure 3 This is a schematic diagram showing the overall growth of Liangwang tea seedlings 68 days after sowing in experimental group 2 of this invention. Figure 4 This is a schematic diagram showing the overall growth of Liangwang tea seedlings 68 days after sowing in experimental group 3 of this invention. Figure 5 This is a schematic diagram showing the overall growth of Liangwang tea seedlings 68 days after sowing in experimental group 4 of this invention. Figure 6 This is a schematic diagram showing the overall growth of Liangwang tea seedlings 68 days after sowing in experimental group 5 of this invention. Figure 7 This is a schematic diagram showing the overall growth of Liangwang tea seedlings 68 days after sowing in experimental group 6 of this invention. Detailed Implementation
[0016] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.
[0017] The raw materials used in the following examples were prepared as follows: 1. Defatted Liangwang Tea Seeds: The seeds are placed in a 0.12% sodium carbonate solution and rubbed repeatedly until the oil on the seed surface is completely washed off, and then rinsed with clean water to obtain defatted Liangwang tea seeds.
[0018] 2. Cooked barley flour: Hulled barley is roasted at 160℃ for 10 minutes, then ground into powder and passed through a 10-mesh sieve to obtain cooked barley flour.
[0019] 3. Barley flour: Hulled barley is dried at 45°C, ground into powder, and passed through a 10-mesh sieve to obtain barley flour.
[0020] 4. Birch Fermented Material A: After cutting birch twigs into 1-3cm sections, soak them in a citric acid solution with a pH of 3.5 for 20 minutes. Then rinse them with clean water until the pH is neutral to obtain pretreated birch twigs. Add twice the volume of clean water and grind them into a slurry. Then inoculate the pretreated birch twigs with 0.2% yeast (saccharomyces cerevisiae, purchased from Shandong Xinzhuoyuan Biotechnology Co., Ltd.) by weight and ferment for 52 hours to obtain fermented material for later use. Then boil the fermented material to a boil and keep it warm for 9 minutes for sterilization. Finally, dry it to obtain birch fermented material A.
[0021] 5. Birch fermented material B: Grind birch twigs into a paste by adding twice the volume of water, then inoculate with yeast at 0.2% of the total mass of the birch twigs, and ferment for 52 hours to obtain fermented material for later use; then boil the fermented material to a boil and keep it warm for 9 minutes for sterilization, and then dry to obtain birch fermented material B.
[0022] 6. Manure: Manure is obtained by mixing fresh sheep manure with corn stalks at a mass ratio of 4:1, composting for 100 days, and then drying.
[0023] Example 1: 1. Seed pretreatment: Method 1: Mix river sand and cooked barley flour at a mass ratio of 8:1 to obtain a compound material. Bury the defatted Liangwang tea seeds in the compound material for 12 days, controlling the humidity at 15% during the burial period. After that, take them out, clean them, and soak them in a 500-fold dilution of carbendazim for 10 minutes to obtain pretreated seeds A.
[0024] Method 2: Mix river sand and barley flour at a mass ratio of 8:1 to obtain a compound material. Bury the defatted Liangwang tea seeds in the compound material for 12 days, controlling the humidity at 15% during the burial period. After that, take them out, clean them, and soak them in a 500-fold dilution of carbendazim for 10 minutes to obtain pretreated seeds B.
[0025] Method 3: The defatted Liangwang tea seeds were buried in river sand for 12 days, with the humidity controlled at 15% during the burial period. After that, they were taken out, cleaned, and then soaked in a 500-fold dilution of carbendazim for 10 minutes to obtain pretreated seeds C.
[0026] 2. Preparation of seedling substrate: Seedling substrate A: Mix the following raw materials in the indicated weight proportions: 20 parts garden soil, 11 parts birch fermented material A, and 5 parts manure.
[0027] Seedling substrate B: Mix the following raw materials in the indicated weight proportions: 20 parts garden soil, 11 parts birch fermented substrate B, and 5 parts manure.
[0028] Seedling substrate C: Mix the following raw materials in the indicated weight parts: 20 parts garden soil and 5 parts manure.
[0029] 3. Nutrient solution preparation: Nutrient solution A: Calcium nitrate 0.9g / L, potassium nitrate 0.7g / L, potassium dihydrogen phosphate 0.4g / L, magnesium sulfate 0.4g / L, boric acid 4mg / L, manganese sulfate 1.5mg / L, disodium EDTA 15mg / L, soybean seed polysaccharide 6mg / L, add water to make up to 1L.
[0030] Nutrient solution B: Calcium nitrate 0.9g / L, potassium nitrate 0.7g / L, potassium dihydrogen phosphate 0.4g / L, magnesium sulfate 0.4g / L, boric acid 4mg / L, manganese sulfate 1.5mg / L, disodium ethylenediaminetetraacetate 15mg / L, add water to make up to 1L.
[0031] 4. Sowing experiment: Experimental method: After UV disinfection of the seedling substrate, each group was placed in a non-woven fabric seedling bag (5cm×12cm) with a filling depth of 7-8cm. Different pretreated seeds were placed in the seedling bag (1 seed per bag), and then covered with 2-3cm of seedling substrate. Then, 0.5-1cm of pine needles were placed on top of the seedling substrate. Nutrient solution was watered thoroughly. After the seeds germinated, the pine needles were removed, and nutrient solution was watered again. Throughout the seedling period, water was applied in a timely manner to maintain the moisture content of the seedling substrate at 50%-70%.
[0032] Following the experimental methods described above, different experimental groups were set up using different seedling substrates, nutrient solutions, and pretreated seeds, as shown in Table 1 below: Table 1
[0033] The experiment was conducted in a greenhouse with a temperature of 15-25℃. 500 seeds were sown in each experimental group, and the germination rate was measured at 20, 35, and 50 days after sowing. The specific results are shown in Table 2 below. Table 2
[0034] As shown in the table above, experimental groups 1, 2 and 3 basically emerged completely by 35 days (emergence rate of over 80% is considered complete emergence), while experimental groups 4-6 had poor early emergence, especially experimental group 6 which emerged the latest overall.
[0035] The growth performance of seedlings in each group was tested 68 days after planting. Five seedlings were taken from each group, and the average plant height, stem diameter, root length, and number of lateral roots were measured and calculated. The specific results are shown in Table 3 below. Table 3
[0036] Refer to this application Figures 1-7 As shown in Table 3 above, the seedlings in experimental groups 1 and 2 basically met the standard for robust seedlings. However, the number of lateral roots in experimental group 1 was significantly greater than that in experimental group 2. Experimental group 3 had fewer lateral roots, with only vigorous development of fibrous roots. The plant height and stem diameter were also lower than those in experimental group 1, especially the plant height. The overall growth of experimental group 4 was worse than that of experimental group 1. The overall growth of seedlings in experimental groups 5 and 6 was lagging behind, and the number of lateral roots in experimental group 5 was also reduced.
[0037] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims
1. A method for promoting vigorous seedling growth of Liangwang tea, characterized in that, The method includes the following steps: (1) Seed pretreatment: Mix river sand and cooked barley flour in a mass ratio of 6-10:1 to obtain compound material. Bury the defatted Liangwang tea seeds in the compound material for 10-15 days. During the burial period, control the humidity at 10%-20%. Then take them out and clean them to obtain pretreated seeds. (2) Prepare seedling substrate according to the following weight parts: 15-25 parts garden soil, 10-12 parts birch fermented material, and 4-6 parts manure; wherein the birch fermented material is prepared by soaking birch dead branches in a solution with a pH of 3.0-3.5, then fermenting them with yeast, then boiling and sterilizing them, and then drying them. (3) Prepare the nutrient solution according to the following ingredients: calcium nitrate 0.9-1.0 g / L, potassium nitrate 0.6-0.8 g / L, potassium dihydrogen phosphate 0.3-0.4 g / L, magnesium sulfate 0.4-0.5 g / L, boric acid 3-5 mg / L, manganese sulfate 1-2 mg / L, disodium ethylenediaminetetraacetate 10-20 mg / L, soybean seed polysaccharide 4-8 mg / L, and add water to make up to 1 L; (4) Sowing: After disinfecting the pretreated seeds, sow them into the seedling substrate, cover them with a layer of pine needles, and water them thoroughly with nutrient solution. After the seedlings emerge, remove the pine needles and water them with nutrient solution again.
2. The method for promoting vigorous seedling growth of Liangwang tea according to claim 1, characterized in that: In step (1), the method for degreasing the Liangwang tea seeds is to place the seeds in a sodium carbonate solution with a mass concentration of 0.8%-0.15% and rub them repeatedly until the oil on the surface of the seeds is completely washed off, and then rinse them clean with water.
3. The method for promoting vigorous seedling growth of Liangwang tea according to claim 1, characterized in that: The cooked barley flour is made by roasting hulled barley at 140-180℃ for 8-10 minutes, then grinding it into powder and passing it through a 10-mesh sieve.
4. The method for promoting vigorous seedling growth of Liangwang tea according to claim 1, characterized in that, The specific preparation method of the birch fermented material includes the following steps: S1. Cut birch dead branches into sections and soak them in a citric acid solution with a pH of 3.0-3.5 for 20-30 minutes. Then rinse with clean water until the pH is neutral to obtain pretreated birch branches. S2. Grind the pretreated birch branches into a slurry with 2-3 times the volume of water, then inoculate with 0.1%-0.2% of the total mass of the pretreated birch branches with yeast, and ferment for 48-60 hours to obtain fermented material for later use. S3. Boil the fermented material until it boils, then keep it warm for 8-10 minutes for sterilization, and then dry it to obtain birch fermented material.
5. The method for promoting vigorous seedling growth of Liangwang tea according to claim 4, characterized in that: In step S1, the length of the birch twig cuttings is 1-3 cm.
6. The method for promoting vigorous seedling growth of Liangwang tea according to claim 1, characterized in that: In step (2), the manure is prepared by mixing fresh animal manure and straw at a mass ratio of 3-5:1 and composting for 90-120 days.
7. The method for promoting vigorous seedling growth of Liangwang tea according to claim 1, characterized in that: In step (4), the pretreatment of seeds is carried out by soaking the pretreated seeds in a 500-fold dilution of carbendazim for 10-15 minutes.
8. The method for promoting vigorous seedling growth of Liangwang tea according to claim 1, characterized in that: In step (4), water should be applied in time before and after seed germination to ensure that the moisture content of the seedling substrate is 50%-70%.
9. The method for promoting vigorous seedling growth of Liangwang tea according to claim 1, characterized in that: In step (4), after the pretreated seeds are sown, they are covered with a 2-3cm layer of seedling substrate, and a 0.5-1cm layer of pine needles is placed on top of the seedling substrate.