An endogenous synergistic extract from natural selenium-enriched black soybean sprouts, its preparation method and application

Abstract

This invention discloses a natural selenium-enriched black soybean sprout endogenous synergistic extract, its preparation method, and its applications, belonging to the fields of food biotechnology and functional foods. Using high-selenium naturally enriched black soybeans as a single raw material, with no exogenous selenium added throughout the process, anthocyanins are enriched through light-protected germination. This is combined with enzymatic hydrolysis and membrane separation purification to construct a multi-component endogenous synergistic system. The extract is rich in highly converted organic selenium, small molecule peptides, anthocyanins, and endogenous phytase, exhibiting characteristics such as resistance to gastric acid, slow release into the intestine, and promotion of mineral absorption. Relying on the synergistic effects of multiple metabolic regulatory pathways, the product can achieve liver protection and detoxification, cell repair, antioxidant effects, and fat reduction. It effectively improves the shortcomings of traditional compound products, such as weak stability, low absorption rate, and large batch-to-batch variations. The production process is stable, with significant industrialization advantages, and it can be widely applied in the fields of functional foods, special dietary foods, and dietary supplements.

Description

Technical Field

[0001] This invention relates to the fields of food biotechnology and functional food products that are both food and medicine, specifically to an extract based on the endogenous biotransformation of natural selenium-rich black soybeans and its multi-target synergistic application in liver protection, fat reduction, and anti-oxidation. Background Technology

[0002] Selenium is an essential trace element for the human body, and black beans are rich in anthocyanins and protein; the combination of the two has significant health benefits. However, current technology has the following significant drawbacks: 1. Risks of exogenous addition: Inorganic selenium salt soaking or physical compounding are often used, which poses a risk of inorganic selenium accumulation toxicity. In addition, each component acts independently and lacks chemical bonding at the molecular level, resulting in low bioavailability.

[0003] 2. Neglecting endogenous activity: Existing technologies often focus only on the extraction of a single component, ignoring the interaction network between endogenous phytase, free amino acids, and small molecule peptides produced by selenium-enriched black beans during germination.

[0004] 3. Batch fluctuation: The selenium content of natural agricultural products has inherent fluctuations, making it difficult to reproduce product indicators during industrial production.

[0005] 4. Poor stability: Anthocyanins are chemically active and are easily oxidized and deactivated in vitro and in gastrointestinal fluids, making them difficult to be effectively absorbed and utilized.

[0006] Therefore, developing a selenium-enriched product that achieves multi-component endogenous synergy through biotransformation, is safe and efficient, and can be compatible with raw material fluctuations is a technical problem that urgently needs to be solved in this field. Summary of the Invention

[0007] This invention aims to overcome the shortcomings of existing technologies and provide a natural selenium-enriched black soybean sprout endogenous synergistic extract and its preparation method. This method, through a specific light-protected germination process, induces biotransformation within the raw material, constructing a multidimensional synergistic network. While ensuring safe dosage, it achieves multi-target physiological regulatory functions and perfectly accommodates batch-to-batch variations in natural raw materials.

[0008] 1. Product Technical Solution An endogenous synergistic extract from natural selenium-enriched black soybean sprouts is prepared by germinating natural selenium-enriched black soybeans in the dark, followed by complex enzymatic hydrolysis and membrane separation purification. The extract contains organic selenium, soybean sprout peptides, black soybean anthocyanins, chelated trace elements, and natural free amino acids.

[0009] Key parameters: raw material selenium content 90-120mg / kg; finished product organic selenium 40-50μg / g; small molecule peptides ≥85%; anthocyanins 1-3.5mg / g.

[0010] Structural characteristics: The components form nano-self-assembled micelles through hydrophobic interactions and hydrogen bonds, without physical mixing. It contains endogenous phytase produced during the germination process, and the components form nanoscale self-assembled micelle structures through hydrophobic interactions and hydrogen bonds.

[0011] The extract uses soybean sprout peptides as its structural framework, linking organic selenium and anthocyanins through hydrogen bonds. It can resist gastric acid degradation and deliver targeted, sustained release in the intestine. Endogenous phytase hydrolyzes phytic acid, breaking the blockade on mineral absorption and synergistically enhancing the bioavailability of trace elements.

[0012] 2. Method and Technical Solution The process includes light-protected germination, compound enzymatic hydrolysis, 1000Da ultrafiltration membrane separation, and freeze-drying.

[0013] The light-protected germination environment is 22~28℃, humidity 85%~90%, for 48~60 hours, and the compound enzymatic hydrolysis is alkaline + flavor protease.

[0014] 3. Multidimensional endogenous synergistic mechanism 1. Structural sustained release: Peptides, selenium, and anthocyanins form nanomicelles that resist gastric acid and achieve targeted release into the intestines; 2. Liver protection and detoxification: Organic selenium and soybean sprout peptides synergistically activate the glutathione peroxidase system; 3. Antioxidant and fat reduction: High anthocyanin content strongly inhibits fat synthesis through the AMPK and SREBP1C pathways; 4. Enzyme activation: Endogenous phytase hydrolyzes phytic acid, improving the absorption and utilization rate of minerals.

[0015] Supplementary explanation of overall logical relationship The core logic of raw materials: All core components are endogenous enriched extracts of selenium-enriched black soybean sprouts, with no exogenous additives. All components revolve around "selenium-enriched black soybean sprouts". The germination process of selenium-enriched black soybeans is the core link. It not only produces selenium-enriched black soybean sprout peptides through enzymatic hydrolysis, but also activates endogenous phytase and activates the complex trace elements enriched by itself. At the same time, it naturally enriches anthocyanins and water-soluble dietary fiber, and synthesizes endogenous organic selenium. This achieves efficient utilization of endogenous raw materials and natural synergy of components, fundamentally avoiding compatibility issues of exogenous added components.

[0016] The component linkage logic is as follows: using selenium-enriched black soybean sprout peptides produced by endogenous enzymatic hydrolysis as the core carrier, it links with endogenous organic selenium and anthocyanins synthesized by itself to form three basic synergistic dimensions: liver protection and detoxification, cell repair, and structural sustained release, realizing the reuse and linkage of endogenous components; taking endogenous phytase as the breakthrough point, it links with endogenous active amino acids produced by enzymatic hydrolysis and self-enriched complex trace elements to solve the problem of mineral absorption and provide endogenous nutritional support for the efficacy of each dimension; finally, by regulating the intestinal flora through endogenous selenium-enriched black soybean sprout peptides and water-soluble dietary fiber, combined with the gut-liver axis linkage, it amplifies the whole-body efficacy, forming a complete logical closed loop of endogenous raw material activation - endogenous carrier delivery - endogenous functional synergy - endogenous efficacy amplification. All linkages are based on the natural characteristics of endogenous components, with no exogenous components involved.

[0017] The efficacy progression logic is as follows: from basic cellular level liver protection and detoxification, and cell repair, to metabolic level lipid metabolism regulation and mineral absorption, then to the efficacy maintenance level of structural slow release, and finally to the overall physiological level of flora regulation and systemic efficacy amplification. Each dimension progresses step by step and supports each other. All efficacy relies on the synergistic effect of the endogenously enriched extract of selenium-enriched black soybean sprouts, ensuring the systematic and effective combined action of multiple components and avoiding the isolated existence of single efficacy. At the same time, each dimension revolves around the core of endogenous synergy, highlighting the natural advantages of the components of selenium-enriched black soybean sprouts, realizing a complete endogenous closed loop of raw materials-components-efficacy, and improving the safety and compatibility of the overall system.

[0018] Molecular pathway regulation mechanism At the microscopic molecular level, the extract of this invention achieves a three-dimensional attack from signal modulation and synthesis inhibition to oxidation promotion: Signal regulation: Small molecule peptides can regulate metabolic pathways such as PI3K / AKT and improve insulin resistance; Synthesis inhibition: Anthocyanins reduce de novo fat synthesis by activating AMPK and inhibiting SREBP1C expression; Oxidation promotion: Selenium components promote fatty acid β-oxidation through the selenoprotein H / PPARα axis.

[0019] Through the synergistic effect of the above-mentioned multi-target therapy, this invention significantly enhances the effects of lipid regulation, weight loss, stomach protection, anti-oxidation, liver protection, kidney protection, and immune enhancement. It can be widely used in the fields of weight management, gastrointestinal health, liver care, kidney protection, anti-aging, and immune enhancement.

[0020] Preparation method 1. Raw material selection: Natural selenium-rich black soybeans are selected, with a selenium content of 90-120mg / kg, and no exogenous selenium is added throughout the process; 2. Germination in the dark: Place soybeans in a completely dark environment at 22-28℃ and 85%-90% humidity for 48-60 hours until the sprouts are 0.5-1.5cm long. This blocks photosynthesis to induce anthocyanin synthesis and promotes the conversion of inorganic selenium to organic selenium. 3. Pulping and homogenization: Mix selenium-enriched black soybean sprouts with pure water at a material-to-liquid ratio of 1:8-10 and pulverize, adjusting the pH to 7.0-7.5; 4. Compound enzymatic hydrolysis: Alkaline protease and flavor protease are added sequentially, with the total enzyme addition amount being 0.5%-1.5% of the raw material mass. Enzymatic hydrolysis is carried out at 50-55℃ for 3-4 hours, and enzyme inactivation is carried out at 90-95℃ for 10-15 minutes. 5. Membrane separation and purification: Centrifuge to collect the supernatant, filter it through a 1000 Da ultrafiltration membrane, collect the permeate and dry it to obtain a powdered extract.

[0021] The selenium element in black beans is mainly concentrated in the high protein component. Through process screening and targeted separation, the high selenium-rich high protein component in black beans is extracted. After removing high selenium and high protein impurities, the final product is refined to obtain selenium-enriched black bean sprout peptide powder with a selenium content stable in the range of 40-50 μg / g.

[0022] application The extract of this invention can be used as a core active ingredient in the preparation of functional foods, special dietary foods or dietary supplements, including dosage forms such as capsules, tablets and powders, for potent antioxidant, liver protection, weight management and fat reduction.

[0023] The beneficial effects of this invention are as follows: The light-shielded germination method of this invention produces anthocyanins at 1-3.5 mg / g, which is more than 10 times that of light-induced germination; the organic selenium enrichment factor is ≥8 times, which is safe and controllable; no exogenous selenium is added throughout the process, and the finished product's organic selenium is controlled at 40-50 μg / g. A daily intake of 3-7g can supplement 120-350 μg of organic selenium, perfectly matching the safe selenium supplementation range for the human body; the nano-micelle structure results in an anthocyanin degradation rate of only 15%, which is far superior to physical compounding; the proportion of small molecule peptides is ≥85%, and endogenous phytase enhances mineral absorption; it uses a single natural raw material, with no inorganic selenium residue, and has strong batch stability; it can be used in functional foods, special dietary foods, and dietary supplements, and also has liver-protecting, antioxidant, and fat-reducing effects.

[0024] The above description is merely an overview of the technical solution of the present invention. In order to better understand the technical means of the present invention and to implement it in accordance with the contents of the specification, the preferred embodiments of the present invention are described in detail below. Detailed Implementation

[0025] The specific embodiments of the present invention will be described in further detail below with reference to the examples. These examples are for illustrative purposes only and are not intended to limit the scope of the invention.

[0026] Unless otherwise specified, the technical solutions described in this invention are all conventional solutions in the field; unless otherwise specified, the reagents or materials described are all from commercial sources.

[0027] Example 1: Preparation using standard process 1. Raw material: Selenium-enriched black soybeans, selenium content 100mg / kg; 2. Germination in the dark: 24℃, 88% humidity, completely dark cultivation for 52 hours, sprout length 0.8cm; 3. Pulping and homogenization: material-to-liquid ratio 1:9, pH 7.3; 4. Compound enzymatic hydrolysis: Total enzyme addition 0.7%, enzymatic hydrolysis at 52℃ for 3.5h, enzyme inactivation at 92℃ for 12min; 5. Membrane separation and drying: 1000 Da ultrafiltration, drying to obtain a dark purple powder.

[0028] Test results: Organic selenium 44μg / g, small molecule peptides accounted for 90.1%; anthocyanins 1.3mg / g.

[0029] Example 2: Validation of Low-Selenium Raw Materials The raw material has a selenium content of 95 mg / kg, and the process is the same as in Example 1.

[0030] Test results: Organic selenium 41μg / g, small molecule peptides 89.5%, anthocyanins 1.2mg / g, core indicators met the standards.

[0031] Example 3: Verification of High-Selenium Raw Materials The raw material has a selenium content of 100 mg / kg, and the process is the same as in Example 1.

[0032] Test results: Organic selenium 48μg / g, small molecule peptides 90.5%, anthocyanins 1.3mg / g, anthocyanin enrichment was not affected by the selenium content of the raw material.

[0033] Comparative Example 1: Germination Control under Light Germination occurs under natural light, and the remaining processes are the same as in Example 1.

[0034] Test results: Anthocyanin 0.32mg / g, bitter taste.

[0035] Comparative Example 2: Physical Compound Control Commercially available organic selenium, soybean peptides, and black soybean anthocyanins were physically mixed according to the proportions in Example 1.

[0036] Test results: Anthocyanins in simulated gastrointestinal fluid degraded by 80% in 30 minutes, and the stability was far lower than that of the extract of this invention.

[0037] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0038] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these all fall within the protection scope of the present invention. Therefore, the protection scope of this invention patent should be determined by the appended claims.

Claims

1. A natural selenium-enriched black soybean sprout endogenous synergistic extract, characterized in that: The extract is prepared from natural selenium-enriched black soybeans with a selenium content of 90-120 mg / kg through light-protected germination, compound enzymatic hydrolysis, and membrane separation purification. The extract is a non-physically mixed endogenous synergistic system containing organic selenium, soybean sprout peptides, black soybean anthocyanins, chelated trace elements, and natural free amino acids. The extract contains 40-50 μg / g of organic selenium, ≥85% of peptides with a molecular weight <1000 Da, and 1-3.5 mg / g of anthocyanins. Each component forms a stable associated structure through endogenous biotransformation during germination, without the addition of exogenous selenium or chemical induction throughout the entire process.

2. The natural selenium-enriched black soybean sprout endogenous synergistic extract according to claim 1, characterized in that: The soybean sprout peptides serve as the structural framework, connecting organic selenium, anthocyanins, and trace elements through hydrophobic interactions and hydrogen bonds to form a nanoscale self-assembled micelle structure. This structure can resist gastric acid degradation and achieve targeted sustained release in the intestine.

3. The natural selenium-enriched black soybean sprout endogenous synergistic extract according to claim 1, characterized in that: It also contains endogenous phytase produced during germination, which breaks the blockade of phytic acid on mineral absorption and synergistically enhances the bioavailability of trace elements.

4. The natural selenium-enriched black soybean sprout endogenous synergistic extract according to claim 1, characterized in that: The natural free amino acids and trace elements form amino acid-metal chelates, which act as metabolic signaling molecules to participate in the regulation of lipid metabolism and the improvement of insulin sensitivity.

5. The use of the natural selenium-enriched black soybean sprout endogenous synergistic extract according to any one of claims 1-4 in the preparation of food, functional food, special dietary food or dietary supplement.

6. The application according to claim 5, characterized in that: The extract exerts its effects through the following multi-dimensional endogenous synergistic mechanisms: Synergistic liver protection and detoxification: Organic selenium is embedded in peptide chains, which synergistically activate the glutathione peroxidase system with soybean sprout peptides; Synergistic cell repair: Soybean sprout peptides mediate anthocyanin transmembrane transport, scavenge mitochondrial free radicals, and participate in damaged tissue remodeling; Synergistic regulation of lipid metabolism: Amino acid-trace element chelates regulate the insulin signaling pathway and optimize the lipid metabolism environment; Synergistic structure-controlled release: The nanomicelle structure protects the active ingredients from the acidic environment of the stomach, enabling targeted release and long-lasting effects.