An automated nucleic acid detection system and method suitable for field conditions

Abstract

This invention, entitled "An Automated Nucleic Acid Detection System and Method Suitable for Field Conditions," belongs to the field of nucleic acid detection technology. The technical problem it aims to solve is the significant susceptibility of existing automated nucleic acid amplification analyzers to environmental factors, poor compatibility of consumables, and unreasonable power supply strategies when used in field conditions. Furthermore, existing improvement schemes are prone to conflict with core patents of the equipment. The key technical solution involves adding a field environment adaptation and control module that communicates with an embedded host computer without altering the core structure of the detection unit. This module collects environmental parameters in real time and dynamically adjusts operating parameters, pre-stores parameter configuration schemes for typical field environments, optimizes the structure and pre-loaded reagent design of the supporting microfluidic detection chip, and adds an emergency power supply optimization unit to dynamically allocate power. Simultaneously, it designs an adapted detection method, including steps such as environmental parameter acquisition, parameter adaptation, automated detection, and result output, adapting to the detection needs of complex field environments.

Description

Technical Field

[0001] This invention belongs to the field of nucleic acid detection technology, specifically relating to an automated nucleic acid detection system and method suitable for field conditions. Background Technology

[0002] Nucleic acid detection technology is one of the core technologies for pathogen detection. Among them, automated nucleic acid amplification analyzers based on magnetic bead nucleic acid extraction and real-time fluorescence PCR have been widely and maturely applied in civilian fields such as clinical diagnosis and public health emergency response because they can realize a fully automated process from sample processing to output of test results.

[0003] With the advancement of national defense construction and the improvement of the field medical support system, the demand for environmental and personnel pathogen detection under field conditions is increasing. Rapid and accurate detection of respiratory and digestive tract pathogens has become an important direction for field medical support. The aforementioned automated nucleic acid amplification analyzer, with its portability and automation advantages, has become an important candidate device for field pathogen detection. However, the design and optimization of existing such devices are based on conventional civilian scenarios, and their direct application to field conditions has many obvious limitations, failing to meet the special detection needs of field scenarios. Specifically, these include:

[0004] (1) The field environment is complex and variable. Special environmental factors such as low temperature and low air pressure, high temperature and high humidity, and bumps and vibrations can easily cause the operating parameters of existing automated nucleic acid amplification analyzers to become inaccurate, which directly affects the efficiency of nucleic acid extraction and the stability of the PCR amplification process, thereby greatly reducing the accuracy of the detection results and making it difficult to meet the accuracy requirements of pathogen detection under field conditions.

[0005] (2) The existing equipment’s consumables are not customized for field scenarios, and do not have pre-loaded specific detection reagents for common respiratory and digestive pathogens in the field. Furthermore, the consumables lack a pollution-proof and easy-to-operate adaptable structure, which not only increases the operational complexity of sampling and detection under field conditions, but also easily leads to sample contamination problems, and cannot meet the core needs of rapid sampling and rapid detection in field scenarios.

[0006] (3) In field conditions, power supply resources are limited and power supply stability is poor. The power supply strategy of the existing automated nucleic acid amplification analyzer does not prioritize and optimize the key steps of the detection process. There is no power supply guarantee mechanism for the core steps. It is easy to cause the core detection process such as nucleic acid extraction and PCR amplification to be terminated due to insufficient power supply or power interruption, resulting in detection failure.

[0007] In view of this, the present invention is hereby proposed. Summary of the Invention

[0008] To address the aforementioned technical problems in the existing technology, this invention provides an automated nucleic acid detection system and method suitable for field conditions. This solves the problems of existing automated nucleic acid amplification analyzers being greatly affected by environmental factors, having poor compatibility with supporting consumables, having unreasonable power supply strategies, and having existing improvement schemes that are prone to conflict with the core patents of the equipment when used in field conditions.

[0009] To achieve the above objectives, the technical solution of the present invention is as follows: Firstly, an automated nucleic acid detection system suitable for field conditions includes an automated nucleic acid amplification and analysis unit based on magnetic bead-based nucleic acid extraction and real-time fluorescence PCR principles, and a matching microfluidic detection chip, comprising: A field environment adaptation and control module is provided, which is connected to the embedded host computer of the automated nucleic acid amplification and analysis unit. It is used to dynamically adjust the operating parameters of the automated nucleic acid amplification and analysis unit according to the field environment parameters. The field environment adaptation and control module has at least two parameter configuration schemes for typical field environments pre-stored.

[0010] Furthermore, the field environment adaptation and control module includes: Environmental parameter acquisition unit: used to acquire temperature, humidity, air pressure and vibration frequency signals; Parameter adaptation unit: used to compare the collected environmental parameters with the pre-stored thresholds and send the matched parameter configuration scheme to the embedded host computer. The operating parameters include sample processing sequence, temperature control threshold compensation amount and fluorescence detection signal gain.

[0011] Furthermore, the typical field environment includes: low temperature and low pressure environment, high temperature and high humidity environment and bumpy and vibration environment. The matching microfluidic detection chip is pre-loaded with specific primer and probe combinations for common respiratory and digestive tract pathogens in the field. The chip is equipped with a pollution-proof sealed interface, which is compatible with the sample injection channel of the automated nucleic acid amplification and analysis unit.

[0012] Furthermore, the specific primer and probe combination is fixed in the reaction chamber of the matching microfluidic detection chip by a freeze-drying process, and the matching microfluidic detection chip is preserved by a vacuum sealing method.

[0013] Furthermore, the field environment adaptation and control module also includes an emergency power supply optimization unit, which is connected to the built-in rechargeable lithium battery of the automated nucleic acid amplification and analysis unit. Under the control of the embedded host computer, the emergency power supply optimization unit dynamically allocates the power supply of the built-in rechargeable lithium battery according to the priority of the detection task.

[0014] Secondly, an automated nucleic acid detection method suitable for field conditions, applied to the aforementioned automated nucleic acid detection system suitable for field conditions, includes: S1. Activate the field environment adaptation and control module to collect the current field environment parameters; S2. Compare the collected field environment parameters with the pre-stored thresholds, match the corresponding parameter configuration scheme, generate the operating parameter adjustment instructions of the automated nucleic acid amplification analysis unit, and send them to the embedded host computer. S3. Inject the sample to be tested into the matching microfluidic detection chip through the anti-contamination sealed interface, and load the matching microfluidic detection chip containing the sample to be tested into the automated nucleic acid amplification and analysis unit; S4. The embedded host computer controls the operation of the automated nucleic acid amplification and analysis unit according to the operating parameter adjustment instructions, and sequentially completes sample lysis, nucleic acid extraction and purification and real-time fluorescence PCR amplification and detection. During the process, fluorescence detection signals are collected in real time and gain calibration is performed. S5. Generate and output the detection results based on the calibrated fluorescence detection signal, and simultaneously generate and store the detection report.

[0015] Furthermore, in step S2, when the collected field environment parameters exceed the pre-stored threshold range, the parameter adaptation unit generates an emergency adjustment command, which includes extending the duration of the incubation step in the sample processing sequence. At the same time, the temperature control threshold compensation amount was adjusted to .

[0016] Furthermore, in step S4, when the collected field environment parameters match the parameter configuration scheme of the bumpy and vibration environment, the embedded host computer controls the ultrasonic component and magnetic component of the automated nucleic acid amplification and analysis unit to reduce the operating power and extend the magnetic bead adsorption time.

[0017] Furthermore, in step S1, the environmental parameter acquisition unit acquires data at a frequency of 1 time / second, and after continuous acquisition for a preset duration, the average value of the acquired data is taken as the current field environment parameter. In step S4, the real-time fluorescence PCR amplification detection process involves performing temperature cycling according to the compensated temperature control threshold to complete the amplification for a preset number of cycles.

[0018] Further, in step S5, the fluorescence detection signals of each reaction chamber are analyzed to obtain the fluorescence signals of each chamber. Value, based on preset The detection results are determined by the threshold value and fluorescence curve morphology. The detection report includes information on the detected object, detection time, current field environment parameters, detection results and judgment basis. The detection report supports local storage and export via external interface.

[0019] The beneficial effects of this invention are as follows: (1) By adding a field environment adaptation and control module, this application can realize the real-time acquisition of field environment parameters and the dynamic adjustment of the operating parameters of the automated nucleic acid amplification and analysis unit. It can adapt to a variety of typical field environments such as low temperature and low pressure, high temperature and high humidity, and bumps and vibrations, effectively improving the stability of the detection system and the accuracy of the detection results in complex field environments. (2) The microfluidic detection chip of this application is preloaded with specific primer and probe combinations for common respiratory and digestive pathogens in the field. It uses freeze-drying process to fix reagents and sets up anti-contamination sealed interface, which not only meets the room temperature storage and transportation needs of field logistics, but also avoids sample contamination, simplifies the operation process, and improves the targeting and operation convenience of nucleic acid detection in the field scenario. (3) This application sets up an emergency power supply optimization unit in the field environment adaptation and control module, which can dynamically allocate the power supply of the built-in rechargeable lithium battery according to the priority of the detection task, and prioritize the power supply stability of the core detection steps, effectively avoiding the detection failure caused by limited power supply resources and power interruption under field conditions. (4) The overall technical solution of this application only achieves field environment adaptation by adding a field environment adaptation and control module, optimizing the design of supporting consumables and the detection process, without changing the core component structure of the automated nucleic acid amplification and analysis unit. It effectively avoids conflicts with subsequent core patent applications of the equipment, can accurately match the project cooperation needs related to field pathogen detection, and meets the requirements of cooperation applications. Attached Figure Description

[0020] Figure 1 A flowchart of an automated nucleic acid detection method suitable for field conditions provided in an embodiment of the present invention. Detailed Implementation

[0021] The technical solution of the present invention will be clearly described below with reference to the accompanying drawings. Obviously, the described embodiments are not all embodiments of the present invention. All other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present invention.

[0022] It should be noted that, unless otherwise specifically stated, the relative arrangement and numerical expressions of the components and steps described in these embodiments should not be construed as limiting the scope of the invention.

[0023] The following description of exemplary embodiments is merely illustrative and is not intended to limit the invention or its application or use in any way. Techniques, methods, and apparatus known to those skilled in the art may not be discussed in detail herein, but where applicable, such techniques, methods, and apparatus should be considered part of this specification.

[0024] Example 1 This invention proposes an automated nucleic acid detection system suitable for field conditions, comprising an automated nucleic acid amplification and analysis unit, a matching microfluidic detection chip, and a field environment adaptation and control module. The field environment adaptation and control module is communicatively connected to the embedded host computer of the automated nucleic acid amplification and analysis unit, and is used to dynamically adjust the operating parameters of the automated nucleic acid amplification and analysis unit according to field environment parameters. These operating parameters include sample processing sequence, temperature control threshold compensation, and fluorescence detection signal gain. Furthermore, the field environment adaptation and control module pre-stores at least two parameter configuration schemes for typical field environments, specifically low-temperature and low-pressure environments, high-temperature and high-humidity environments, and bumpy and vibrating environments. M1, Automated Nucleic Acid Amplification and Analysis Unit: An integrated nucleic acid amplification and analysis instrument based on the principle of magnetic bead nucleic acid extraction and real-time fluorescence PCR. Its core components have not undergone structural changes, specifically including micro-pump valve assembly, ultrasonic and magnetic suction assembly, semiconductor cooling temperature control assembly, laser diode light source, miniature spectrometer sensor and embedded host computer; The laser diode light source works in conjunction with the miniature spectrometer sensor for real-time acquisition of fluorescence detection signals, while the semiconductor cooling chip temperature control component is used to achieve temperature cycling regulation during the PCR amplification process. The unit is equipped with a touch screen for human-computer interaction, output of detection results, and input of operation commands. A built-in rechargeable lithium battery powers the entire unit. The overall size is slightly larger than the palm of your hand, and it weighs about 1.1kg, making it suitable for portable use in the field.

[0025] M2, Field Environment Adaptation and Control Module: Includes an environmental parameter acquisition unit, a parameter adaptation unit, and an emergency power supply optimization unit. Each unit works together to achieve environmental parameter acquisition, parameter adaptation and matching, and dynamic allocation of power supply. M21, Environmental Parameter Acquisition Unit: Used to acquire temperature, humidity, air pressure and vibration frequency signals; The unit employs miniature temperature and humidity sensors, barometric pressure sensors, and vibration sensors. The temperature and humidity sensor measures from -40℃ to 85℃ and humidity from 0 to 100%RH, the barometric pressure sensor measures from 30kPa to 110kPa, and the vibration sensor measures from 0 to 200Hz. This unit is used to acquire temperature, humidity, barometric pressure, and vibration frequency signals. The sensors are connected to the parameter adapter unit via an SPI interface to ensure the real-time performance and accuracy of parameter acquisition.

[0026] M22, Parameter Adaptation Unit: Used to compare the collected environmental parameters with the pre-stored thresholds and send the matched parameter configuration scheme to the embedded host computer. The operating parameters include sample processing sequence, temperature control threshold compensation amount and fluorescence detection signal gain. Specifically, a microcontroller is used as the core control component. It pre-stores parameter configuration schemes for three typical field environments: low temperature and low pressure, high temperature and high humidity, and bumpy vibration. It is used to compare the environmental parameters collected by the environmental parameter acquisition unit with the pre-stored thresholds, match the corresponding parameter configuration scheme, and send it to the embedded host computer. This unit communicates with the embedded host computer of the automated nucleic acid amplification and analysis unit via a serial port, and the three pre-stored typical environment parameter configuration schemes are as follows: Low temperature and low pressure scheme (temperature ≤ -10℃, pressure ≤ 60kPa): The incubation step in the sample processing sequence is extended by 20%, the temperature control threshold compensation is increased by 1.0℃, and the fluorescence detection signal gain is improved by 15%; High temperature and high humidity protocol (temperature ≥35℃, humidity ≥85%RH): The incubation step in the sample processing sequence is extended by 10%, the temperature control threshold compensation is -0.5℃, and the fluorescence detection signal gain is increased by 10%. Bump and vibration scheme (vibration frequency ≥ 50Hz): The operating power of ultrasonic and magnetic components is reduced by 30%, the magnetic bead adsorption time is extended by 25%, and the temperature control threshold compensation is ±0.8℃.

[0027] M23, Emergency Power Supply Optimization Unit: Connected to the built-in rechargeable lithium battery of the automated nucleic acid amplification and analysis unit, it is used to dynamically allocate the power supply of the built-in rechargeable lithium battery according to the priority of the detection task under the control of the embedded host computer; Specifically, under the control of the embedded host computer, the power supply of the built-in rechargeable lithium battery is dynamically allocated according to the priority of the detection task, so as to give priority to ensuring the power supply stability of the sample lysis and nucleic acid extraction and purification steps. This unit divides power supply priority into three levels, with the highest level (level 1) being the sample lysis and nucleic acid extraction and purification steps, where power supply is guaranteed to be ≥80%. The second stage is the PCR amplification step, and the power supply must be ≥60%. Level 3 is the result processing and output step, with power supply guaranteed at ≥40%.

[0028] M3, with matching microfluidic detection chip: made of polymethyl methacrylate (PMMA) material, with 1 sample inlet, 6 reaction chambers and corresponding flow channel structure; the sample inlet is equipped with a silicone anti-contamination sealing interface, which is compatible with the sample inlet channel of the automated nucleic acid amplification analysis unit, and can avoid sample contamination during sampling and loading. The chip's six reaction chambers are pre-loaded with specific primer and probe combinations for common respiratory and digestive pathogens in the field, specifically specific primer and probe combinations for respiratory syncytial virus, influenza virus, norovirus, Salmonella, Shigella, and internal reference genes. The specific primer and probe combination is fixed inside the reaction chamber by a lyophilization process. The reagent volume in each chamber is 20 μL. The entire chip is lyophilized and then stored in a vacuum-sealed manner, which can realize long-term room temperature storage of reagents without the need for cold chain transportation.

[0029] Example 2 See Figure 1 , Figure 1 This embodiment provides a flowchart of an automated nucleic acid testing method suitable for field conditions, applied to the automated nucleic acid testing system for field conditions described in Embodiment 1. Through a standardized process of environmental parameter acquisition, parameter adaptation, sample loading, automated testing, and result output and storage, nucleic acid testing in complex field environments is achieved. Specific parameter adjustment strategies are set for extreme environments and bumpy / vibrating environments. The specific steps include: S1. Environmental Parameter Acquisition: Start the field environment adaptation and control module to collect the current field environment parameters; After the detection system is started, the environmental parameter acquisition unit of the field environment adaptation and control module starts working, collecting the current environmental temperature, humidity, air pressure and vibration frequency parameters in real time. The acquisition frequency is 1 time / second, and after 30 seconds of continuous acquisition, the average value of the acquired data is taken as the current field environment parameter to ensure the accuracy of the acquired parameters.

[0030] S2. Parameter Adaptation and Adjustment: The collected field environment parameters are compared with the pre-stored thresholds, the corresponding parameter configuration scheme is matched, and the operating parameter adjustment instructions of the automated nucleic acid amplification analysis unit are generated and sent to the embedded host computer. The parameter adaptation unit compares the collected field environment parameters with the pre-stored thresholds, matches the corresponding parameter configuration scheme, generates operating parameter adjustment instructions including sample processing sequence, temperature control threshold compensation amount and fluorescence detection signal gain, and sends the instructions to the embedded host computer of the automated nucleic acid amplification analysis unit. If the collected field environment parameters exceed the pre-stored threshold range, the parameter adaptation unit directly generates an emergency adjustment command. This command includes extending the incubation step in the sample processing sequence by 10%-30% and adjusting the temperature control threshold compensation to ±0.5-1.5℃.

[0031] S3. Sample loading: The sample to be tested is injected into the matching microfluidic detection chip through the anti-contamination sealed interface, and the matching microfluidic detection chip loaded with the sample to be tested is loaded into the automated nucleic acid amplification and analysis unit. Collect the sample to be tested and prepare the sample eluent. Inject the sample eluent into the sample inlet of the matching microfluidic detection chip through the anti-contamination sealed interface. After injection, press the sealing cap to seal the chip. Load the matching microfluidic detection chip containing the sample to be tested into the chip slot of the automated nucleic acid amplification analysis unit along the guide rail. After the touch screen shows that loading is complete, proceed to the next detection process.

[0032] S4. Detection Operation: The embedded host computer controls the automated nucleic acid amplification and analysis unit to run according to the operation parameter adjustment instructions, and sequentially completes sample lysis, nucleic acid extraction and purification and real-time fluorescence PCR amplification and detection. During the process, fluorescence detection signals are collected in real time and gain calibration is performed. The embedded host computer adjusts the received operating parameters according to the instructions, and controls the automated nucleic acid amplification and analysis unit to run according to the adjusted parameters. It sequentially completes sample lysis, nucleic acid extraction and purification, and real-time fluorescence PCR amplification and detection. Throughout the process, fluorescence detection signals are collected in real time and gain calibration is performed. If the collected field environment parameters match the parameter configuration scheme of the bumpy and vibration environment, the ultrasonic component and magnetic component of the embedded host computer synchronously control the automated nucleic acid amplification and analysis unit to reduce the operating power by 30%, while extending the magnetic bead adsorption time by 25% and adjusting the temperature control threshold compensation to ±0.8℃ to ensure the efficiency of nucleic acid extraction and purification. The real-time fluorescence PCR amplification detection process involves the semiconductor cooling chip temperature control component performing temperature cycling according to the compensated temperature control threshold. The specific temperature cycling parameters are 95℃ denaturation for 30 seconds and 58℃ annealing extension for 45 seconds, completing a total of 40 cycles of amplification. The laser diode light source and the miniature spectrometer sensor collect fluorescence detection signals in real time throughout the process, and complete signal calibration according to the adjusted gain parameters.

[0033] S5. Result Output: Generate and output the detection result based on the calibrated fluorescence detection signal, and generate and store the detection report.

[0034] After PCR amplification and detection are completed, the embedded host computer analyzes the calibrated fluorescence detection signal. Based on the Ct value and fluorescence curve morphology of each reaction chamber obtained from the analysis, the detection result is determined. The determination criteria are: if the Ct value of a certain chamber is ≤38 and the fluorescence curve is a typical S-shape, it is determined to be positive; otherwise, it is negative. After the determination is completed, the detection result is output through the touch screen, and a detection report conforming to the field operation record specifications is generated. The report includes the detection personnel information, detection time, current field environment parameters, detection results of each pathogen and determination criteria, and the detection report is stored in the local storage module of the embedded host computer. This detection report can be exported to external devices via USB interface.

[0035] The above specific embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to examples, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the scope of the technical solutions of the present invention, and all such modifications or substitutions should be covered within the scope of the claims of the present invention.

Claims

1. An automated nucleic acid detection system suitable for field conditions, comprising an automated nucleic acid amplification and analysis unit based on magnetic bead-based nucleic acid extraction and real-time fluorescence PCR, and a matching microfluidic detection chip, characterized in that, include: A field environment adaptation and control module is provided, which is connected to the embedded host computer of the automated nucleic acid amplification and analysis unit. It is used to dynamically adjust the operating parameters of the automated nucleic acid amplification and analysis unit according to the field environment parameters. The field environment adaptation and control module has at least two parameter configuration schemes for typical field environments pre-stored.

2. The automated nucleic acid testing system suitable for field conditions according to claim 1, characterized in that, The field environment adaptation and control module includes: Environmental parameter acquisition unit: used to acquire temperature, humidity, air pressure and vibration frequency signals; Parameter adaptation unit: used to compare the collected environmental parameters with the pre-stored thresholds and send the matched parameter configuration scheme to the embedded host computer. The operating parameters include sample processing sequence, temperature control threshold compensation amount and fluorescence detection signal gain.

3. The automated nucleic acid testing system suitable for field conditions according to claim 1, characterized in that, The typical field environment includes: low temperature and low pressure environment, high temperature and high humidity environment and bumpy and vibration environment. The matching microfluidic detection chip is pre-loaded with specific primer and probe combinations for common respiratory and digestive tract pathogens in the field. The chip is equipped with a pollution-proof sealed interface, which is compatible with the sample injection channel of the automated nucleic acid amplification and analysis unit.

4. The automated nucleic acid testing system suitable for field conditions according to claim 3, characterized in that, The specific primer and probe combination is fixed in the reaction chamber of the matching microfluidic detection chip by a freeze-drying process, and the matching microfluidic detection chip is preserved by a vacuum sealing method.

5. The automated nucleic acid testing system suitable for field conditions according to claim 1, characterized in that, The field environment adaptation and control module also includes an emergency power supply optimization unit, which is connected to the built-in rechargeable lithium battery of the automated nucleic acid amplification and analysis unit. The emergency power supply optimization unit is used to dynamically allocate the power supply of the built-in rechargeable lithium battery according to the priority of the detection task under the control of the embedded host computer.

6. An automated nucleic acid detection method suitable for field conditions, characterized in that, An automated nucleic acid testing system suitable for field conditions, applicable to any one of claims 1-5, comprises: S1. Activate the field environment adaptation and control module to collect the current field environment parameters; S2. Compare the collected field environment parameters with the pre-stored thresholds, match the corresponding parameter configuration scheme, generate the operating parameter adjustment instructions of the automated nucleic acid amplification analysis unit, and send them to the embedded host computer. S3. Inject the sample to be tested into the matching microfluidic detection chip through the anti-contamination sealed interface, and load the matching microfluidic detection chip containing the sample to be tested into the automated nucleic acid amplification and analysis unit; S4. The embedded host computer controls the operation of the automated nucleic acid amplification and analysis unit according to the operating parameter adjustment instructions, and sequentially completes sample lysis, nucleic acid extraction and purification and real-time fluorescence PCR amplification and detection. During the process, fluorescence detection signals are collected in real time and gain calibration is performed. S5. Generate and output the detection results based on the calibrated fluorescence detection signal, and simultaneously generate and store the detection report.

7. The automated nucleic acid detection method suitable for field conditions according to claim 6, characterized in that, In step S2, when the collected field environment parameters exceed the pre-stored threshold range, the parameter adaptation unit generates an emergency adjustment command, which includes extending the incubation step duration in the sample processing sequence. At the same time, the temperature control threshold compensation amount was adjusted to .

8. The automated nucleic acid detection method suitable for field conditions according to claim 6, characterized in that, In step S4, when the collected field environment parameters match the parameter configuration scheme of the bumpy and vibration environment, the embedded host computer controls the ultrasonic component and magnetic component of the automated nucleic acid amplification and analysis unit to reduce the operating power and extend the magnetic bead adsorption time.

9. The automated nucleic acid detection method suitable for field conditions according to claim 6, characterized in that, In step S1, the environmental parameter acquisition unit acquires data at a frequency of 1 time / second, and after continuous acquisition for a preset duration, the average value of the acquired data is taken as the current field environment parameter. In step S4, the real-time fluorescence PCR amplification detection process involves performing temperature cycling according to the compensated temperature control threshold to complete the amplification for a preset number of cycles.

10. The automated nucleic acid detection method suitable for field conditions according to claim 6, characterized in that, In step S5, the fluorescence detection signals of each reaction chamber are analyzed to obtain the fluorescence signals of each chamber. Value, based on preset The detection results are determined by the threshold value and fluorescence curve morphology. The detection report includes information on the detected object, detection time, current field environment parameters, detection results and judgment basis. The detection report supports local storage and export via external interface.

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