A non-heme chloride peroxidase and its application
By heterologously expressing and purifying non-heme chloride peroxidase RoCPO, the problem of lacking efficient catalysts in existing technologies was solved, enabling the efficient oxidation of HMF and FFCA and improving the biosynthetic efficiency of FDCA.
CN122303175APending Publication Date: 2026-06-30QILU UNIVERSITY OF TECHNOLOGY (SHANDONG ACADEMY OF SCIENCES)
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Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- QILU UNIVERSITY OF TECHNOLOGY (SHANDONG ACADEMY OF SCIENCES)
- Filing Date
- 2026-04-22
- Publication Date
- 2026-06-30
AI Technical Summary
Technical Problem
The lack of efficient enzyme catalysts in the current technology for catalyzing the oxidation of 5-hydroxymethylfurfural (HMF) and 5-formyl-2-furancarboxylic acid (FFCA) to 2,5-furandicarboxylic acid (FDCA) limits the efficiency of FDCA biosynthesis.
Method used
By heterologously expressing and purifying the non-heme chloride peroxidase RoCPO (derived from *Rauvolobacterium ornithine-derived*), and performing in vitro enzymatic catalysis, the oxidation of HMF and FFCA to HMFCA and FDCA was achieved.
Benefits of technology
This enriches the enzyme resource library for the biosynthesis of FDCA, improves the conversion rate of HMF and FFCA, and provides a new biocatalytic synthesis pathway.
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Figure CN122303175A_ABST
Abstract
This invention provides a non-heme chloride peroxidase and its application, belonging to the field of genetic engineering. The non-heme chloride peroxidase... Ro CPO, whose amino acid sequence is SEQ ID NO.2; the non-heme chlorperoxidase Ro The nucleotide sequence of the CPO encoding gene is SEQ ID NO.1; the non-heme chlorperoxidase... Ro Application of CPO in the preparation of 5-hydroxymethyl-2-furanic acid and / or 2,5-furanic acid.
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