A method and application for target gene quantification in qPCR using quantitative internal standards and comprehensive correction coefficients.

By using a quantitative internal standard and a comprehensive correction coefficient K value, the problem of standard curve dependence in qPCR is solved, achieving efficient and accurate nucleic acid quantification without the need for a standard curve, which is suitable for POCT environments.

CN122303395APending Publication Date: 2026-06-30VIRTUE DIAGNOSTICS (SUZHOU) CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
VIRTUE DIAGNOSTICS (SUZHOU) CO LTD
Filing Date
2026-04-01
Publication Date
2026-06-30

AI Technical Summary

Technical Problem

Existing qPCR methods require the establishment of a standard curve for each experiment, which increases reagent costs and the complexity of the experimental procedure, and results in poor detection repeatability, especially in the POCT environment where efficiency is low.

Method used

By using a quantitative internal standard with a known copy number and a global comprehensive correction coefficient K, a single calibration experiment is conducted to encapsulate and compensate for systematic biases, thereby achieving precise quantification of the target gene.

Benefits of technology

Accurate quantification of target nucleic acids can be achieved without the need for a standard curve, improving the reliability and repeatability of detection results. This method is suitable for point-of-care testing scenarios, reduces costs, and simplifies experimental procedures.

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Abstract

This invention, entitled "A Method and Application for Target Gene Quantification in qPCR Using a Quantitative Internal Standard and a Comprehensive Correction Coefficient," belongs to the field of nucleic acid detection technology. The technical problem it addresses is that existing qPCR techniques require the establishment of a standard curve using a reference sample of known concentration, increasing reagent costs and experimental complexity, and resulting in poor reproducibility. The key technical solution is to provide a method for quantitative viral load qPCR analysis of a single sample. Specifically, it provides an internal standard with a known copy number, i.e., a quantitative internal standard, and establishes a correction method. Through a systematic error compensation mechanism, it achieves accurate quantification of the target gene. This method ensures both accuracy and reproducibility while simplifying detection design in clinical applications.
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