A walnut seedling grafting compatibility rapid identification device and identification method

Abstract

This invention discloses a rapid identification device and method for graft compatibility of walnut seedlings in the field of walnut planting detection technology. The method includes: S1: placing and fixing the grafting site of the walnut seedling in a sealed detection chamber; S2: activating a gas detection module to detect the concentration of oxygen and carbon dioxide in the sealed detection chamber in real time, while an environmental detection module detects the temperature and humidity inside the sealed detection chamber; S3: transmitting the detection data from the gas detection module and the environmental detection module to a data processing module; S4: calculating the rate of change of oxygen and carbon dioxide based on the received data, and correcting the data according to temperature and humidity; S5: comparing the corrected gas rate of change with a preset gas rate of change threshold range to obtain the identification result of the graft compatibility of the walnut seedling. This invention rapidly identifies compatibility by detecting the rate of change of gas at the grafting site, shortening the cycle and providing accurate and stable results.

Description

Technical Field

[0001] This invention relates to the field of walnut planting and testing technology, specifically to a rapid identification device and method for graft compatibility of walnut seedlings. Background Technology

[0002] Walnuts, considered a "golden tree species" in my country's economic forestry sector, are not only a source of high-quality woody oil and dried fruit, but their timber is also a scarce material for military and high-end furniture manufacturing. With the surge in consumer demand for high-quality walnut varieties, large-scale propagation of superior seedlings has become a core driving force for industrial upgrading, and grafting technology is a key means to achieve walnut trait improvement and germplasm innovation. However, traditional walnut grafting faces the technical bottleneck of rootstock-scion incompatibility. Due to significant differences in genetic background among walnut species and varieties, grafting often results in physiological metabolic imbalances and poor vascular bundle connections between the rootstock and scion, leading to low survival rates of grafted seedlings. Even some surviving seedlings may experience growth decline and fruit quality deterioration after 3-5 years, exhibiting "late-stage incompatibility." Therefore, grafting compatibility assessment methods are used to evaluate the compatibility at the graft union, allowing for timely treatment of grafted walnut plants with low compatibility.

[0003] Existing identification methods include spectral analysis, bioelectrical impedance analysis, and physiological and biochemical index detection. However, these methods also have some drawbacks. For example, while spectral analysis is fast, establishing a mathematical model requires a large amount of sample data, and the spectral characteristics are easily variable under different growth environments, requiring repeated optimization and calibration. Bioelectrical impedance analysis requires applying alternating current at different frequencies and analyzing the frequency domain response, while thermal imaging technology requires strict control of environmental conditions and interpretation of thermal images; both methods place high demands on the operator's technical skills. Physiological and biochemical index detection methods are easily affected by the environment and the plant's own condition, molecular biological identification methods cannot fully reflect the actual compatibility after grafting, and thermal imaging technology is greatly affected by environmental factors.

[0004] Therefore, this invention proposes a rapid identification device and method for graft compatibility of walnut seedlings to solve the above problems. Summary of the Invention

[0005] To address the aforementioned problems, this invention provides a rapid identification device and method for grafting compatibility of walnut seedlings. By detecting the rate of gas change at the grafting site, compatibility can be rapidly identified, shortening the time required and providing accurate and stable results.

[0006] To achieve the above objectives, the technical solution of the present invention is as follows: A rapid identification device for graft compatibility of walnut seedlings includes a sealed detection chamber, which is composed of two sealed shells, both of which are semi-ellipsoidal structures. The inner wall of each sealed shell is provided with a variable layer, which is made of rubber. Fixed airbags are provided at both ends of the sealed shells, and pump assemblies are provided inside each sealed shell. The pump assemblies are connected to the fixed airbags. A control screen is provided on the outer surface of one of the sealed shells.

[0007] The control panel is internally connected to a data processing module, and the inner wall of the sealed housing is equipped with a gas detection module and an environmental detection module that are electrically connected to the data processing module.

[0008] Furthermore, each gas detection module includes an oxygen sensor and a carbon dioxide sensor, both of which are located on the inner wall of the sealed housing and are electrically connected to the data processing module.

[0009] Furthermore, each environmental monitoring module includes a temperature sensor and a humidity sensor, both of which are electrically connected to the data processing module and are located on the inner wall of the sealed housing.

[0010] Furthermore, a rapid method for identifying grafting compatibility in walnut seedlings includes the following steps:

[0011] S1: Place the grafting site of the walnut seedling in a sealed testing chamber and fix it in place;

[0012] S2: The gas detection module is activated to monitor the concentration of oxygen and carbon dioxide in the sealed detection chamber in real time, while the environmental detection module monitors the temperature and humidity in the sealed detection chamber.

[0013] S3: The gas detection module and the environmental detection module transmit the detection data to the data processing module;

[0014] S4: The data processing module calculates the rate of change of oxygen and carbon dioxide based on the received data, and corrects the data based on temperature and humidity.

[0015] S5: The data processing module compares the corrected gas change rate with the preset gas change rate threshold range to obtain the identification results of the grafting compatibility of walnut seedlings.

[0016] Furthermore, prior to step S1, a step of performing a sealing test on the sealing test chamber is included, as detailed below:

[0017] A fixed amount of standard gas is introduced into the sealing test chamber. After standing for a preset time, the change in gas concentration in the sealing test chamber is detected. If the change in gas concentration is less than the preset leakage threshold, the sealing test chamber is judged to be in good condition.

[0018] Furthermore, in step S4, the data processing module calculates the oxygen change rate and carbon dioxide change rate according to the following formulas:

[0019] Oxygen change rate = (initial oxygen concentration during detection time - final oxygen concentration during detection time) / detection time;

[0020] The rate of change of carbon dioxide = (final value of carbon dioxide concentration within the detection time - initial value of carbon dioxide concentration within the detection time) / detection time.

[0021] Furthermore, in step S5, the preset gas change rate threshold range is determined as follows:

[0022] Several groups of walnut seedlings with known good and poor graft compatibility were selected and tested under the same environmental conditions. The gas change rate data obtained from the tests were statistically analyzed to determine the threshold range of gas change rate that distinguishes between good and poor graft compatibility.

[0023] Furthermore, in step S5, when the gas change rate is within a preset range, the grafting compatibility of the walnut seedlings is determined to be good; when the gas change rate exceeds the preset range, the grafting compatibility of the walnut seedlings is determined to be poor.

[0024] Furthermore, in step S5, the data processing module integrates the identification results, detection time, environmental parameters, and gas change rate to generate a detection report, which is then wirelessly transmitted through the control screen and synchronized to the user terminal. At the same time, it is automatically stored in the local database for historical data comparison and analysis.

[0025] Furthermore, in step S4, the data correction specifically includes the following sub-steps:

[0026] a. Obtain the real-time temperature value T detected by the temperature sensor and the real-time humidity value H detected by the humidity sensor;

[0027] b. Establish a mapping table for temperature correction coefficient K1 and humidity correction coefficient K2 based on historical experimental data;

[0028] c. Corrected rate of oxygen change = Original rate of oxygen change × K1 × K2;

[0029] d. Corrected rate of change of carbon dioxide = original rate of change of carbon dioxide × K1 × K2.

[0030] The beneficial effects of the above scheme are as follows: 1. This invention directly quantifies the intensity of plant respiration metabolism by monitoring the oxygen consumption and carbon dioxide release rates at the grafting site in real time. After grafting, rootstock-scion combinations with good compatibility will rapidly initiate cell repair and vascular bundle connection, manifested as characteristic changes in respiration rate; conversely, incompatible combinations exhibit abnormal metabolic activity, with gas change rates deviating from the normal pattern. This detection method based on fundamental physiological processes overcomes the lag of traditional morphological observation, enabling identification within hours to days after grafting, significantly shortening the breeding cycle. Simultaneously, through environmental parameter correction and threshold comparison algorithms, interference from external factors such as temperature and humidity is eliminated, ensuring the accuracy and stability of the results.

[0031] 2. This solution employs a combination structure of a semi-ellipsoidal sealing shell and an elastic fixing airbag, which can adaptively adjust according to the seedling diameter, avoiding mechanical damage while ensuring the sealing of the test. The double-shell design facilitates quick installation and disassembly, making it suitable for large-scale field operations. The integrated environmental monitoring module monitors microenvironmental changes in real time, and the data processing module automatically completes parameter correction and result determination, reducing reliance on the operator's professional knowledge. In addition, the control screen intuitively displays the test data and identification results, enabling visualized operation and further improving work efficiency.

[0032] Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0033] Figure 1 This is a schematic diagram of the rapid identification method for grafting compatibility of walnut seedlings according to the present invention; Figure 2 This is an overall isometric view of an embodiment of the rapid identification device for grafting compatibility of walnut seedlings according to the present invention; Figure 3 This is an overall side sectional view of an embodiment of the rapid identification device for graft compatibility of walnut seedlings according to the present invention.

[0035] The reference numerals in the accompanying drawings include: 1. Sealing shell; 2. Change layer; 3. Fixed airbag; 4. Fixed base. Detailed Implementation

[0036] The technical solution of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0037] In the description of this invention, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing the invention and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0038] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0039] The following detailed description illustrates the specific implementation method:

[0040] Example 1:

[0041] As attached Figure 1 and Figure 2 As shown: A rapid identification device for graft compatibility of walnut seedlings includes a sealed detection chamber, which is composed of two sealed shells 1. Both sealed shells 1 have a semi-ellipsoidal structure, and the inner wall of each sealed shell 1 is provided with a change layer 2, which is made of rubber. Fixed airbags 3 are provided at both ends of each sealed shell 1, and a pump assembly (preferably an air pump) is provided inside each sealed shell 1. The pump assembly is connected to the fixed airbag 3. A control screen is provided on the outer surface of one of the sealed shells 1. In addition, the control screen is preferably a touch screen. At the same time, the pump assembly is electrically connected to the control screen so that the operator can control it through the control screen.

[0042] The control panel is electrically connected to a data processing module, and the inner wall of the sealed shell 1 is equipped with a gas detection module and an environmental detection module that are electrically connected to the data processing module.

[0043] Specifically, each gas detection module includes an oxygen sensor and a carbon dioxide sensor, both of which are disposed on the inner wall of the sealed housing 1 and are electrically connected to the data processing module. Each environmental detection module includes a temperature sensor and a humidity sensor, both of which are electrically connected to the data processing module and are disposed on the inner wall of the sealed housing 1.

[0044] like Figure 1As shown, several fixed seats 4 are integrally formed on both sides of the sealing shell 1. After the two sealing shells 1 are aligned, bolts are installed on the corresponding fixed seats 4 to fix the two sealing shells 1 together, thereby forming a sealed testing chamber. At the same time, sealing grooves and rubber sealing strips are provided on the opposite sides of the two sealing shells 1 to ensure that a sealed space is formed inside the sealed testing chamber.

[0045] Example 2:

[0046] like Figure 3 As shown, a rapid method for identifying grafting compatibility in walnut seedlings includes the following steps:

[0047] S1: After the walnut seedling grafting is completed, align the two sealing shells 1 and place them on the grafting position. Then, fix the two sealing shells 1 with bolts to form a sealed detection chamber. Then, start the pump assembly (air pump) through the control panel to inflate the fixed airbags 3 at both ends of the sealed detection chamber, so that they fit tightly against the branches. This increases the stability by increasing the coefficient of friction. At the same time, the flexibility of the fixed airbags 3 is used to adapt to the contour of the walnut grafting position.

[0048] In addition, before S1, it is necessary to conduct an airtightness test on the installed device to improve the accuracy of the gas change rate in the subsequent test chamber, thereby improving the accuracy of the compatibility identification after walnut grafting. Therefore, it is necessary to conduct a sealing test on the test chamber. The specific steps are as follows: fill the test chamber with a certain amount of standard gas, let it stand for a preset time, and then test the change in gas concentration in the test chamber. If the change in gas concentration is less than the preset leakage threshold, the test chamber is judged to be in good sealing condition.

[0049] S2: Use oxygen and carbon dioxide sensors to detect the concentration of oxygen and carbon dioxide in the sealed detection chamber in real time, while temperature and humidity sensors detect the temperature and humidity in the sealed detection chamber.

[0050] S3: The gas detection module and the environmental detection module transmit the detection data to the data processing module;

[0051] S4: The data processing module calculates the rate of change of oxygen and carbon dioxide based on the received data, and corrects the data based on temperature and humidity.

[0052] The formulas for calculating the rates of change in oxygen and carbon dioxide are as follows:

[0053] Oxygen change rate = (initial oxygen concentration during detection time - final oxygen concentration during detection time) / detection time;

[0054] The rate of change of carbon dioxide = (final value of carbon dioxide concentration within the detection time - initial value of carbon dioxide concentration within the detection time) / detection time.

[0055] The specific steps for data correction are as follows:

[0056] a. Obtain the real-time temperature value T detected by the temperature sensor and the real-time humidity value H detected by the humidity sensor;

[0057] b. Establish a mapping table of temperature correction coefficient K1 and humidity correction coefficient K2 based on historical experimental data (e.g., K1 = 1.0 when T = 25℃, K1 = 0.95 when T = 30℃; K2 = 1.0 when H = 60%, K2 = 0.98 when H = 70%).

[0058] c. Corrected rate of oxygen change = Original rate of oxygen change × K1 × K2;

[0059] d. Corrected rate of change of carbon dioxide = original rate of change of carbon dioxide × K1 × K2.

[0060] S5: The data processing module compares the corrected gas change rate with the preset gas change rate threshold range to obtain the identification result of the walnut seedling grafting compatibility. When the gas change rate is within the preset range, the walnut seedling is judged to have good grafting compatibility; when the gas change rate exceeds the preset range, the walnut seedling is judged to have poor grafting compatibility. The identification result, detection time, environmental parameters, and gas change rate are then integrated to generate a detection report, which is wirelessly transmitted through the control screen and synchronized to the user terminal. At the same time, it is automatically stored in the local database for historical data comparison and analysis. The steps for determining the preset gas change rate threshold range are as follows: Several groups of walnut seedling samples with known good and poor grafting compatibility are selected and tested under the same environmental conditions using the identification device described in Example 1. The gas change rate data obtained from the test are statistically analyzed to determine the gas change rate threshold range that distinguishes between good and poor grafting compatibility.

[0061] The above-mentioned rapid identification method for grafting compatibility of walnut seedlings is compared with existing walnut compatibility identification techniques, as follows:

[0062] I. Experimental Subjects

[0063] Three common walnut varieties (Ziyue, Mianning Zirong, and Xiangsu No. 1) were selected as scions, and ordinary walnut trees were used as rootstocks. Twenty grafting samples were prepared for each variety, for a total of 60 samples. The grafting samples were randomly divided into two groups of 30 samples each, which were used for the identification method of this invention, the traditional physiological and biochemical index detection method, and the traditional electrical impedance spectroscopy analysis method, respectively.

[0064] II. Experimental Methods

[0065] (I) Identification Method of the Invention

[0066] Device installation and airtightness testing: After grafting, place the sealing test chamber over the grafting site and secure the two sealing shells with bolts. Start the pump assembly to inflate the fixing airbag and make it fit the branch. Fill the sealing test chamber with a quantitative amount of standard gas, let it stand for 30 minutes, and then test the change in gas concentration. If the change is less than the preset leakage threshold (0.5%), the sealing is considered good.

[0067] Data Detection and Analysis: The oxygen, carbon dioxide, temperature, and humidity sensors are activated to monitor gas concentrations and environmental parameters within the sealed detection chamber in real time, recording data every 10 minutes. The data processing module calculates the rates of change for oxygen and carbon dioxide using formulas, corrects the data based on temperature and humidity, and compares it with preset threshold ranges to obtain the grafting compatibility assessment results.

[0068] (II) Traditional Physiological and Biochemical Indicator Detection Methods

[0069] Sample collection: On the 7th day after grafting, tissue samples were collected from the grafting site, and each sample was collected in 3 replicates.

[0070] Indicator determination: The peroxidase (POD) activity, polyphenol oxidase (PPO) activity, soluble sugar content, and soluble protein content in the samples were determined. POD and PPO activities were determined by spectrophotometry, soluble sugar content was determined by the anthrone colorimetric method, and soluble protein content was determined by the Coomassie brilliant blue method.

[0071] Results analysis: Graft compatibility was comprehensively evaluated based on various physiological and biochemical indicators. Different weights were set for each indicator to calculate the compatibility score. A score above 80 was considered good compatibility, and a score below 60 was considered poor compatibility.

[0072] (III) Traditional Electrical Reactivity Spectrum Analysis Method

[0073] Sample preparation: On the second day after grafting the walnut seedlings, select branches 5cm above and below the grafting site as test samples. Wrap each sample with damp gauze to prevent moisture loss from affecting the test results. Take 10 sets of samples for each variety, and measure each set of samples 3 times.

[0074] Equipment Connection and Calibration: Using a professional electrical impedance tomography analyzer, evenly wrap the detection electrodes (four-electrode system) around the grafted branch, maintaining a 1cm spacing between the electrodes. After preheating for 15 minutes, calibrate the equipment using a standard resistor to ensure measurement accuracy.

[0075] Data acquisition: The measurement frequency range was set to 10Hz-1MHz. A measurement point was selected every 10 octaves, and data was collected 5 times for each measurement point, with the average value taken. During the measurement process, the ambient temperature was maintained at 25±1℃ and the humidity at 60±5% to reduce the interference of environmental factors on the electrical impedance parameters.

[0076] Data analysis: The resistance (R), capacitance (C), and impedance (Z) values ​​of the samples at different frequencies were obtained. Principal component analysis (PCA) and partial least squares (PLS) were used to establish a predictive model of the relationship between electrical impedance parameters and grafting compatibility. By calculating the comprehensive score of the samples, a threshold was set (a score ≥75 was considered good compatibility, and <75 was considered poor compatibility) to obtain the grafting compatibility identification results.

[0077] III. Experimental Results

[0078] Table 1 - Comparison of relevant identification parameters for walnut affinity identification

[0079]

[0080] As shown in Table 1, in terms of accuracy, the identification method of this invention achieves an accuracy rate of 90%, 85%, and 95% for the three varieties Ziyue, Mianning Zirang, and Xiangsu No. 1, respectively. This is significantly higher than the 70%-75% of the traditional physiological and biochemical index detection method and the 62%-73% of the electrical impedance spectroscopy analysis method, enabling more accurate screening of rootstock-scion combinations with good compatibility.

[0081] In terms of identification efficiency, the method of this invention can complete the detection in an average of only 24 hours, while the electrical impedance spectroscopy analysis method requires 48 hours, and the traditional physiological and biochemical index detection method takes up to 7 days. This means that this invention can greatly shorten the breeding cycle of walnut varieties, accelerate the breeding process, and reduce time costs. In addition, traditional methods are cumbersome to operate, requiring professional instruments and complex sample processing, and the electrical impedance spectroscopy analysis method also requires specific equipment and data analysis capabilities; while the identification device of this invention has a reasonable structural design, is easy to operate, requires no complicated sample pretreatment, and can be operated by ordinary technicians after simple training, making it more practical and valuable for promotion, and providing more reliable technical support for the large-scale and efficient development of the walnut industry.

[0082] Obviously, the above embodiments are merely illustrative examples for clear explanation and are not intended to limit the implementation. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. However, obvious variations or modifications derived therefrom are still within the scope of protection of this invention.

Claims

1. A rapid identification device for grafting compatibility of walnut seedlings, characterized in that, The system includes a sealed testing chamber, which consists of two sealed shells (1). Both sealed shells (1) are semi-ellipsoidal structures. The inner walls of both sealed shells (1) are provided with a variable layer (2), which is made of rubber. Fixed airbags (3) are provided at both ends of the sealed shells (1). Pump assemblies are provided inside the sealed shells (1), and the pump assemblies are connected to the fixed airbags (3). A control panel is provided on the outer surface of one of the sealed shells (1). The control panel is electrically connected to a data processing module, and the inner wall of the sealed shell (1) is equipped with a gas detection module and an environmental detection module that are electrically connected to the data processing module.

2. The rapid identification device for graft compatibility of walnut seedlings according to claim 1, characterized in that: The gas detection module includes an oxygen sensor and a carbon dioxide sensor. Both the oxygen sensor and the carbon dioxide sensor are located on the inner wall of the sealed shell (1). Both the oxygen sensor and the carbon dioxide sensor are electrically connected to the data processing module.

3. The rapid identification device for grafting compatibility of walnut seedlings according to claim 2, characterized in that: The environmental monitoring module includes a temperature sensor and a humidity sensor. The temperature sensor and humidity sensor are electrically connected to the data processing module and are located on the inner wall of the sealed shell (1).

4. A method for rapid identification of grafting compatibility in walnut seedlings, comprising any one of the rapid identification devices for grafting compatibility in walnut seedlings according to claims 1-3, characterized in that, Includes the following steps: S1: Place the grafting site of the walnut seedling in a sealed testing chamber and fix it in place; S2: The gas detection module is activated to monitor the concentration of oxygen and carbon dioxide in the sealed detection chamber in real time, while the environmental detection module monitors the temperature and humidity in the sealed detection chamber. S3: The gas detection module and the environmental detection module transmit the detection data to the data processing module; S4: The data processing module calculates the rate of change of oxygen and carbon dioxide based on the received data, and corrects the data based on temperature and humidity. S5: The data processing module compares the corrected gas change rate with the preset gas change rate threshold range to obtain the identification results of the grafting compatibility of walnut seedlings.

5. The rapid identification method for grafting compatibility of walnut seedlings according to claim 4, characterized in that: Before step S1, a step of performing a sealing test on the sealing test chamber is also included, as follows: A fixed amount of standard gas is introduced into the sealing test chamber. After standing for a preset time, the change in gas concentration in the sealing test chamber is detected. If the change in gas concentration is less than the preset leakage threshold, the sealing test chamber is judged to be in good condition.

6. The rapid identification method for grafting compatibility of walnut seedlings according to claim 4, characterized in that: In step S4, the data processing module calculates the oxygen change rate and carbon dioxide change rate according to the following formulas: Oxygen change rate = (initial oxygen concentration during detection time - final oxygen concentration during detection time) / detection time; The rate of change of carbon dioxide = (final value of carbon dioxide concentration within the detection time - initial value of carbon dioxide concentration within the detection time) / detection time.

7. The rapid identification method for grafting compatibility of walnut seedlings according to claim 4, characterized in that: In step S5, the steps for determining the preset gas change rate threshold range are as follows: Several groups of walnut seedlings with known good and poor graft compatibility were selected and tested under the same environmental conditions. The gas change rate data obtained from the tests were statistically analyzed to determine the threshold range of gas change rate that distinguishes between good and poor graft compatibility.

8. The rapid identification method for grafting compatibility of walnut seedlings according to claim 4, characterized in that: In step S5, when the gas change rate is within a preset range, the walnut seedling is determined to have good grafting compatibility; when the gas change rate exceeds the preset range, the walnut seedling is determined to have poor grafting compatibility.

9. The rapid identification method for grafting compatibility of walnut seedlings according to claim 4, characterized in that: In step S5, the data processing module integrates the identification results, detection time, environmental parameters, and gas change rate to generate a detection report, which is then wirelessly transmitted through the control panel and synchronized to the user terminal. At the same time, it is automatically stored in the local database for historical data comparison and analysis.

10. The rapid identification method for grafting compatibility of walnut seedlings according to claim 4, characterized in that: In step S4, the data correction specifically includes the following sub-steps: a. Obtain the real-time temperature value T detected by the temperature sensor and the real-time humidity value H detected by the humidity sensor; b. Establish a mapping table for temperature correction coefficient K1 and humidity correction coefficient K2 based on historical experimental data; c. Corrected rate of oxygen change = Original rate of oxygen change × K1 × K2; d. Corrected rate of change of carbon dioxide = original rate of change of carbon dioxide × K1 × K2.

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