A time-resolved fluorescence immunochromatographic kit for the determination of oxidized low-density lipoprotein and its preparation method

By constructing core-shell structured fluorescent microspheres, encapsulating rare earth europium complexes, and modifying their surfaces, the sensitivity and stability issues of OxLDL detection were resolved, achieving highly sensitive and accurate quantitative detection, suitable for time-resolved fluorescence immunochromatographic detection of oxidized low-density lipoprotein.

CN122361797APending Publication Date: 2026-07-10融和生物科技(河南)有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-04-10
Publication Date
2026-07-10

AI Technical Summary

Technical Problem

Existing OxLDL detection methods suffer from low sensitivity, poor stability, difficulty in eliminating background interference, and low labeling efficiency, making it difficult to achieve highly sensitive and accurate quantitative detection.

Method used

A core-shell structured fluorescent microsphere encapsulates the rare earth europium complex Eu(TTA)3(TPPO)2 within a silica core, with a dense silica layer as the outer shell and surface amylation modification. This microsphere is used for antibody conjugation and is then detected using time-resolved fluorescence immunochromatography.

Benefits of technology

It achieves high sensitivity, high stability, and high repeatability in the quantitative detection of OxLDL, with improved quantum yield, 10-fold increase in signal-to-noise ratio, wide linear range, and a detection limit of 2.5 U/L, making it suitable for clinical sample testing.

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Abstract

This invention relates to a time-resolved fluorescence immunochromatographic assay kit for the determination of oxidized low-density lipoprotein (OxLDL) and its preparation method. The kit includes a detection card and a sample diluent. The detection card comprises, from bottom to top, a sample pad, a conjugate pad, a nitrocellulose membrane, absorbent paper, and a PVC base plate. The conjugate pad is coated with a mouse anti-OxLDL monoclonal antibody labeled with fluorescent microspheres. The nitrocellulose membrane has a detection line and a control line. The fluorescent microspheres have a core-shell structure, with a core of mesoporous silica loaded with rare-earth europium complexes and a shell of dense silica. The surface of the fluorescent microspheres is modified with amino groups. This invention constructs a silica-coated core-shell structure, encapsulating high-quantity-yield europium complexes within the core. The silica shell provides protection against external quenching factors while simultaneously providing abundant surface-active groups for antibody conjugation, achieving highly sensitive, stable, and reproducible quantitative detection of OxLDL.
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