A device for identifying authenticity of medicinal materials

Abstract

The application discloses a device for identifying the authenticity of medicinal materials, and belongs to the technical field of traditional Chinese medicinal material quality detection. The device comprises an integrated detection host, a sample pretreatment module, a nucleic acid amplification module, an electrophoresis detection module, a touch display module and a power supply module; the sample pretreatment module is internally provided with a crushing and nucleic acid extraction unit, the nucleic acid amplification module adopts a constant-temperature amplification control structure, the electrophoresis detection module is used for nucleic acid fragment identification, and the whole machine realizes integrated automatic completion of sample processing, nucleic acid amplification, electrophoresis color development and result interpretation. The device does not need to be operated by professional personnel, and has the advantages of rapid detection, high sensitivity and intuitive results, and can be used for rapidly identifying the authenticity of cordyceps sinensis and various valuable medicinal materials, and is suitable for on-site use in market supervision, pharmacies, pharmaceutical enterprises and laboratories.

Description

Technical Field

[0001] This invention relates to the field of equipment for quality testing of Chinese medicinal materials, specifically a device for identifying the authenticity of medicinal materials. Background Technology

[0002] Currently, adulteration is widespread in the market for valuable medicinal materials such as Cordyceps sinensis, American ginseng, and Gastrodia elata. Traditional identification methods are highly subjective and have low accuracy; PCR testing requires multiple devices to operate in stages, which is demanding on personnel, cumbersome, and time-consuming; existing testing equipment is bulky and inconvenient, failing to meet the needs of rapid on-site screening. Therefore, developing an integrated, automated, and portable device for identifying the authenticity of medicinal materials has significant practical value. Summary of the Invention

[0003] The purpose of this invention is to provide a device for identifying the authenticity of medicinal materials, which integrates sample pretreatment, nucleic acid extraction, specific amplification, electrophoresis detection, and automatic result interpretation. It is simple, fast, accurate, portable, and easy to use.

[0004] The technical solution of the present invention is as follows: a device for identifying the authenticity of medicinal materials, comprising a detection host housing, an internal sample pretreatment unit, a nucleic acid amplification unit, an electrophoresis identification unit, a control motherboard, and a power supply unit; and an external touch screen display, a sample inlet, and a detection chamber door.

[0005] The sample pretreatment unit automatically completes the crushing, lysis, extraction, and purification of nucleic acids.

[0006] The nucleic acid amplification unit amplifies specific fragments.

[0007] The electrophoretic identification unit automatically images and compares it with the database to output a true or false conclusion.

[0008] Beneficial effects of this invention: 1. Integrated design eliminates the need for complex operations such as manual pipetting, centrifugation, and electrophoresis.

[0009] 2. The results are automatically interpreted, and can be used directly by non-professionals.

[0010] 3. Portable and mobile, supporting on-site testing in markets, pharmacies, and fields.

[0011] 4. High sensitivity, capable of detecting trace samples, and high accuracy.

[0012] 5. Supports identification of authenticity for various medicinal materials, with strong versatility. Attached Figure Description

[0013] Figure 1 is a schematic diagram of the overall structure of the present invention; Figure 2 is a block diagram of the internal modules of the present invention; Figure 3 is a schematic diagram of the electrophoretic recognition unit structure of the present invention.

[0014] Figure Labels 1. Inspect the main unit casing 2. Touchscreen display 3. Sample inlet 4. Inspect the warehouse door 5. Result Output Port 6. Sample Pretreatment Unit 7. Nucleic acid amplification unit 8. Electrophoretic Recognition Unit 9. Control motherboard 10. Power Supply Unit 11. Ultraviolet light source assembly 12. Image acquisition camera 13. Gel card slot 14. Electrode Assembly Detailed Implementation

[0015] The medicinal sample is placed into the sample inlet, and the device automatically proceeds to crushing, CTAB nucleic acid extraction, and purification. The extracted nucleic acid is then sent to the nucleic acid amplification unit for specific amplification. The amplified product is then sent to the electrophoresis recognition unit for gel electrophoresis. After ultraviolet imaging, the system automatically compares the band characteristics and displays the results on the touch screen. Genuine product: Specific target bands appear. Counterfeit: No corresponding stripe will be generated, and the system will automatically output a authenticity detection report.

Claims

1. A device for identifying the authenticity of medicinal materials, characterized in that, The device includes a detection host housing, inside which are a sample pretreatment unit, a nucleic acid amplification unit, an electrophoresis recognition unit, a control motherboard, and a power supply unit; outside the detection host housing are a touch screen display, a sample inlet, a detection chamber door, and a result output port; the control motherboard is electrically connected to the sample pretreatment unit, the nucleic acid amplification unit, the electrophoresis recognition unit, and the touch screen display.

2. The apparatus according to claim 1, characterized in that, The sample pretreatment unit includes a grinding and crushing chamber, a heating and temperature control component, a CTAB nucleic acid extraction component, a centrifugation drive structure, and a pipeline flow guiding component, which are used to complete the crushing of medicinal materials, nucleic acid lysis, extraction, purification, and collection.

3. The apparatus according to claim 1, characterized in that, The nucleic acid amplification unit includes a constant temperature reaction chamber, a temperature control chip, a reaction tube holder, and a heating and cooling assembly, and is used for specific isothermal amplification of nucleic acid fragments from medicinal materials.

4. The apparatus according to claim 1, characterized in that, The electrophoresis identification unit includes an agarose gel slot, an electrode assembly, an ultraviolet light source assembly, an image acquisition camera, and a data analysis module, which can automatically collect electrophoretic bands and determine the authenticity of the results.

5. The apparatus according to claim 1, characterized in that, The touch screen is used to select the type of medicinal material, start the detection, and display the amplification curve, electrophoresis pattern, and authenticity determination conclusion.

6. The apparatus according to claim 1, characterized in that, The device has a built-in rechargeable lithium battery and power interface, supporting portable mobile on-site testing.

7. The apparatus according to claim 1, characterized in that, The device has a built-in medicinal herb identification database, which includes specific nucleic acid feature information of various precious medicinal herbs such as Cordyceps sinensis, American ginseng, Gastrodia elata, Panax notoginseng, and Dendrobium.

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