Micro-bubble circulation seedling device and kelp seedling method

Abstract

This invention discloses a microbubble circulating seedling collection device and a kelp seedling cultivation method. The microbubble circulating seedling collection device includes a seedling distribution pool, a reciprocating mobile spray seedling distribution system, a shock-absorbing seedling attachment frame, and a microbubble control system. The reciprocating mobile spray seedling distribution system evenly sprays droplets onto the water surface, which are fully mixed with the water during the falling process. Combined with the microbubble control system set at the bottom of the pool, the air flotation effect generated by the microbubble control system keeps the gametophytes suspended in the water, effectively prolonging the settling time of the gametophytes. The vibration of the shock-absorbing seedling attachment frame causes the seedling rope to swing slightly, increasing the probability of collision between gametophyte cells and seedling rope fibers. This invention can significantly improve the seedling attachment efficiency and uniformity, and solve the problems of seedling shortage and seedling drop caused by rapid settling of gametophytes.

Description

Technical Field

[0001] This invention belongs to the field of kelp seedling technology, specifically, it relates to a microbubble circulating seedling collection device and a kelp seedling cultivation method. Background Technology

[0002] Kelp is an important economic algae in my country, widely used in food, medicine, chemical industry, and marine ecological restoration, possessing extremely high nutritional value and promising industrial applications. Kelp seedlings are the core foundation determining the yield and quality of kelp farming, directly affecting farming efficiency and sustainable industrial development; therefore, the industry's demand for high-quality kelp seedlings is increasing daily.

[0003] Currently, kelp seedling propagation technology mainly includes two processes: sporophyte collection and gametophyte cloning. Sporophyte collection utilizes mature sporophytes to release zoospores, which attach and germinate to form gametophytes. The gametophytes then complete sexual reproduction to obtain young sporophytes. This method is simple to operate and is the main process used by most kelp seedling enterprises. However, it is limited by the sporangium maturation time, allowing collection only at specific times in summer. Furthermore, the seedling cultivation cycle is long, making it difficult to guarantee purity and easily leading to germplasm degradation. Gametophyte cloning involves propagating stable female and male gametophytes, allowing them to mix and develop into young sporophytes. This method allows for long-term germplasm preservation, is not limited by season, is flexible and efficient, and produces seedlings with stable traits and excellent germplasm quality, facilitating standardized seedling production. However, its disadvantages include a high technical threshold, unstable attachment, susceptibility to benthic diatom contamination, and a tendency to cause large-scale seedling shortages and dropouts, affecting seedling yield and quality.

[0004] Gametophyte cloning involves shredding gametophyte cells and sprinkling them onto seedling ropes to collect seedlings. However, gametophytes lack the ability to swim and must rely on gravity to settle and attach. Furthermore, the rapid settling after sprinkling makes even distribution on the seedling ropes difficult, resulting in unstable attachment, inconsistent density, and significant localized seedling loss. In the early stages of cultivation after seedling collection, the young sporophytes have weak adhesion. Traditional washing methods to remove diatoms and other algae can easily cause the young sporophytes to detach, leading to seedling loss. Therefore, seedling curtains are often not washed in the early stages of cultivation. However, this allows diatoms and other algae to grow. Once these algae proliferate in large numbers, they compete with gametophytes and young sporophytes for survival, further exacerbating seedling loss.

[0005] Therefore, improving the uniformity and stability of gametophyte attachment and effectively controlling algal contamination without damaging young sporophytes is a bottleneck restricting the large-scale application of gametophyte cloning technology.

[0006] The information disclosed in this background section is only intended to enhance the understanding of the background technology of this application, and therefore may include prior art that is not known to those skilled in the art. Summary of the Invention

[0007] This invention proposes a microbubble circulating seedling collection device to solve the technical problem of unstable and uneven seedling attachment caused by rapid sedimentation of gametophytes during the seedling collection process in existing kelp gametophyte clonal seedling production.

[0008] To achieve the above-mentioned invention / design objectives, the present invention adopts the following technical solution: A microbubble circulating seedling collection device, the device comprising: The seedling pool includes a pool bottom, a first pool wall and a second pool wall, and the pool bottom includes a central pool bottom, a first guide bottom that gradually increases in height from the central pool bottom toward the first pool wall, and a second guide bottom that gradually increases in height from the central pool bottom toward the second pool wall. A reciprocating mobile spraying seedling system, including guide rails and mobile nozzles; The shock-absorbing seedling base frame includes a fixed support frame and a shock-absorbing floating frame located on the fixed support frame. The shock-absorbing floating frame is connected to a waterproof micro shock-absorbing motor. Several shock-absorbing seedling base frames are arranged on the first and second guide bottoms. The microbubble control system is located at the first guide bottom, the middle pool bottom, and the second guide bottom; The controller is configured to control the reciprocating mobile spray seedling system to spray the diluted gamete filtrate in one direction: the spraying is turned on when the mobile nozzle goes out and turned off when it returns, completing one round-trip seedling cycle, and then the next round-trip seedling is performed after an interval; the controller controls the micro-vibration motor to work intermittently: the micro-vibration motor is turned on when the mobile nozzle goes out and turned off when the mobile nozzle returns and during the interval; the controller controls the microbubble control system to work intermittently: the microbubble control system is turned on when the mobile nozzle returns and during the interval, and turned off when the mobile nozzle goes out; after multiple round-trip seedlings, the reciprocating mobile spray seedling system, the microbubble control system, and the micro-vibration motor are turned off, and the seedlings are attached while maintaining still water for a first set time.

[0009] In the microbubble circulating seedling collection device described above, the extension direction of the central pool bottom is the same as the extension direction of the first pool wall and the second pool wall, and the inclination of the first guide bottom and the second guide bottom is 5-8°.

[0010] The microbubble circulating seedling collection device described above, wherein the guide rail includes: A first guide rail located above the first pool wall and a second guide rail located above the second pool wall; The movable nozzle includes: A first movable nozzle is located on the first guide rail and can move along the first guide rail. The first movable nozzle is configured to swing within a specific angle. A second movable nozzle is located on and movable along the second guide rail, and the second movable nozzle is configured to oscillate within a specific angle.

[0011] As described above, in the microbubble circulating seedling collection device, the fixed support frame includes a column and a fixed rectangular frame, and the column is fixed on the first guide bottom or the second guide bottom; The shock-absorbing floating frame includes a floating rectangular frame, which is connected to a waterproof miniature shock-absorbing motor via shock-absorbing elements. A guide rod is installed on one of the fixed rectangular frame and the floating rectangular frame, and a guide sleeve is installed on the other. The guide rod is inserted into the guide sleeve.

[0012] As described above, the microbubble circulating seedling collection device includes a microbubble control system comprising nano-aeration pipes arranged at the first guide bottom, the middle pool bottom, and the second guide bottom, wherein the nano-aeration pipes generate microbubbles with a diameter of less than 50 μm.

[0013] A method for cultivating kelp seedlings based on the above-mentioned microbubble circulating seedling collection device includes the following steps: Install the pretreated seedling curtain onto the shock-absorbing floating frame of the seedling base frame; Seawater is added to the seedling pool until the water level covers the seedling curtain. The prepared gametophyte filtrate is diluted with low-temperature seawater and then sprayed onto the seedlings using the reciprocating mobile spray seedling system. The reciprocating mobile spray seedling system performs unidirectional spraying: the mobile nozzle turns on spraying when going out and turns off spraying when returning, completing one round-trip seedling distribution cycle, and then performs the next round-trip seedling distribution after an interval. The micro vibration damping motor operates intermittently: it turns on when the moving nozzle is going out, and turns off when the moving nozzle is returning and at intervals. The microbubble control system operates intermittently: it is turned on when the moving nozzle returns and at intervals, and turned off when the moving nozzle goes out. After multiple rounds of seedling placement, the reciprocating mobile spray seedling placement system, microbubble control system, and micro vibration damping motor are turned off, and the seedlings are attached in still water for the first set time.

[0014] In the kelp seedling cultivation method described above, when the moving nozzle sprays, the moving nozzle oscillates at a set frequency on its outward journey, and the first moving nozzle and the second moving nozzle move in the same direction and at the same speed, so that the spray overlaps.

[0015] In the kelp seedling cultivation method described above, the method for preparing the gametophyte filtrate is as follows: The female and male gametophytes stored at low temperature were separately added to sterilized ice seawater, chopped up, and inoculated and cultured at a density of 1-1.5 g / L. Culture conditions: temperature 10-15℃, light 1000lx-3500lx, photoperiod L:D=12h:12h, continuous aeration for 24 hours, culture medium is sterile seawater with added NaNO3 and KH2PO4 to concentrations of 10mg / L and 2mg / L respectively, and the culture medium is changed regularly; The amplified gametophyte clones were mixed at a female to male fresh weight ratio of 2:1, sterilized ice seawater was added and chopped, and the mixture was repeated several times. The mixture was then filtered until the cell segments filtered out under a microscope were 1-5 cells.

[0016] The pretreatment method for the seedling curtain in the kelp seedling cultivation method described above is as follows: The seedling curtain is soaked in the mixture for a second set time, and air is continuously injected during soaking; The mixture ratio is: brown sugar 5-10g / L, disodium EDTA 5-10g / L, probiotics 10-20g / L; Preparation order of the mixture and activation of probiotics: First, dissolve brown sugar in a small amount of warm water, add seawater and stir well, then add probiotics, aerate and activate for 2-4 hours, add disodium EDTA, dissolve fully and then put it into the seedling tray.

[0017] As described above, the kelp seedling cultivation method involves the cultivation and management of seedlings after attachment: Water temperature adjustment: 7-11℃; Water flow regulation: Control the microbubble control system to work intermittently. After the third set time of seedling attachment, apply a micro water flow with a velocity of less than 5 cm / s to the seedling pond, turn off the microbubble control system, and after the fourth set time, gradually increase the water flow to the normal seedling water flow. Light regulation: Average light intensity during gametophyte stage: 1700-1800 lx, not exceeding 2000 lx; ​​average light intensity during early sporophyte stage: 1800-2000 lx, not exceeding 3500 lx; ​​average light intensity during late sporophyte stage: 2000-2400 lx, not exceeding 7000 lx. Washing and cleaning the pond: When the sporophyte develops into 2-4 rows of cells, start washing the seedling curtain. In the early stage, clean by vibrating a micro-vibration motor. In the later stage, gradually increase the vibration intensity of the motor. Start cleaning the pond after the fifth set time. Kelp seedlings are ready for shipment when they reach a length of 1-1.2 cm.

[0018] Compared with the prior art, the advantages and positive effects of the present invention are: This invention relates to a microbubble circulating seedling collection device, comprising a seedling distribution pool, a reciprocating mobile spray seedling distribution system, a shock-absorbing seedling attachment frame, and a microbubble control system. The reciprocating mobile spray seedling distribution system evenly sprays droplets onto the water surface, allowing them to mix thoroughly with the water as they fall. Combined with the microbubble control system located at the bottom of the pool, the air flotation effect generated by the microbubble control system keeps the gametophytes suspended in the water, effectively prolonging their settling time. The vibration of the shock-absorbing seedling attachment frame causes the seedling ropes to oscillate slightly, increasing the probability of collision between gametophyte cells and seedling rope fibers. This invention can significantly improve seedling attachment efficiency and uniformity, solving problems such as seedling loss and drop caused by rapid gametophyte settling.

[0019] This invention relates to a kelp seedling cultivation method based on a microbubble circulating seedling collection device. The method involves installing a pre-treated seedling curtain onto a shock-absorbing floating frame of the seedling attachment base; adding seawater to the seedling distribution tank until the water surface covers the seedling curtain; diluting the prepared gametophyte filtrate with low-temperature seawater; and then spraying the solution using a reciprocating mobile spray seedling distribution system. The reciprocating mobile spray seedling distribution system operates unidirectionally: spraying is activated when the nozzle travels outwards and deactivated when it returns, completing one round-trip seedling distribution cycle. After an interval, the next round-trip seedling distribution begins. A micro-shock-absorbing motor operates intermittently: it is activated when the nozzle travels outwards and deactivated when it travels back and during the interval. A microbubble control system operates intermittently: it is activated when the nozzle travels back and during the interval, and deactivated when the nozzle travels outwards. After multiple round-trip seedling distributions, the reciprocating mobile spray seedling distribution system, microbubble control system, and micro-shock-absorbing motor are deactivated, and the seedling attachment is completed within a set time in still water. This invention can significantly improve seedling attachment efficiency and uniformity, and solve problems such as seedling loss and drop caused by rapid gametophyte settling.

[0020] Other features and advantages of the present invention will become clearer after reading the detailed embodiments of the invention in conjunction with the accompanying drawings. Attached Figure Description

[0021] To more clearly illustrate the technical solutions in the embodiments of the present invention, the accompanying drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0022] Figure 1 This is a top view schematic diagram of a microbubble circulating seedling collection device according to a specific embodiment of the present invention.

[0023] Figure 2 This is a side view schematic diagram of a microbubble circulating seedling collection device according to a specific embodiment of the present invention.

[0024] Figure 3This is a schematic diagram of a shock-absorbing seedling support frame according to a specific embodiment of the present invention.

[0025] In the picture: 1. Seedling tray; 11. First guide bottom; 12. Second guide bottom; 13. Middle section of the tray bottom; 2. Reciprocating mobile spraying seedling distribution system; 21. Mobile nozzle; 22. Guide rail; 3. Shock-absorbing seedling base frame; 31. Fixed support frame; 32. Shock-absorbing floating frame; 33. Waterproof miniature shock-absorbing motor; 341. Guide sleeve; 342. Guide rod; 35. Anchor bolts; 4. Microbubble control system. Detailed Implementation

[0026] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0027] In the description of this invention, it should be understood that the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0028] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances. In the description of embodiments, specific features, structures, materials, or characteristics can be combined in any suitable manner in one or more embodiments or examples.

[0029] The terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature.

[0030] In the description of this invention, unless otherwise stated, "a plurality of" means two or more.

[0031] like Figure 1-3 As shown in the figure, this embodiment proposes a microbubble circulating seedling collection device, including a seedling distribution pool, a reciprocating mobile spray seedling distribution system, a shock-absorbing seedling base frame, a microbubble control system, and a controller.

[0032] The seedling pool 1 includes a pool bottom, a first pool wall and a second pool wall, a third pool wall and a fourth pool wall, which are connected sequentially. The pool bottom is connected to the bottom of the first pool wall, the third pool wall, the second pool wall and the fourth pool wall.

[0033] In some embodiments, the first pool wall and the second pool wall have the same length, the third pool wall and the fourth pool wall have the same length, and the length of the first pool wall and the second pool wall is greater than the length of the third pool wall and the fourth pool wall, that is, the seedling pool 1 is rectangular.

[0034] The pool bottom includes a central pool bottom 13 and a first guide bottom 11 that gradually increases in height from the central pool bottom 13 toward the first pool wall, and a second guide bottom 12 that gradually increases in height from the central pool bottom 13 toward the second pool wall.

[0035] The inclined slope of the first guide bottom 11 and the second guide bottom 12 in the pool bottom allows the water flow to be naturally guided along the slope to the middle pool bottom 13, forming a circulation pattern, which makes the water flow in the seedling pool more uniform, and at the same time facilitates the concentration of sediments and the purification of sewage. In some embodiments, the seedling pool 1 is a cement pool, and the bottom of the pool slopes down slowly from the long side pool wall (the first pool wall and the second pool wall) to the middle pool bottom 13 to form a V-shaped guide bottom.

[0036] In some embodiments, the V-shaped seedling tray has dimensions of 10-15m in length, 4-5m in width, and 1-1.2m in height.

[0037] In some embodiments, the extension direction of the middle part of the pool bottom 13 is the same as the extension direction of the first pool wall and the second pool wall, and the inclination of the first guide bottom 11 and the second guide bottom 12 is 5-8°.

[0038] In some embodiments, the central pool bottom 13 is located at the midline between the first pool wall and the second pool wall. In this case, the first guide bottom 11 and the second guide bottom 12 are connected.

[0039] In some embodiments, the first guide bottom 11 and the second guide bottom 12 are connected by a smooth transition portion to form a smooth transition portion, which is very narrow, and the middle pool bottom 13 is the smooth transition portion.

[0040] In some embodiments, the central pool bottom 13 is a groove located between the first guide bottom 11 and the second guide bottom 12, parallel to the long side of the seedling pool 1 (the first pool wall and the second pool wall). For example, the central pool bottom 13 can be a plane, with the bottom ends of the first guide bottom 11 and the second guide bottom 12 connected to the plane, the plane being perpendicular to the first pool wall and the second pool wall, and the first guide bottom 11 and the second guide bottom 12 forming an angle of 5-8° with the plane.

[0041] The reciprocating mobile spray seedling system 2 includes a guide rail 22 and a mobile nozzle 21.

[0042] In some embodiments, the guide rail 22 is a linear guide rail.

[0043] The reciprocating mobile spray seedling system 2 is a dual-track, dual-nozzle system.

[0044] The guide rail 22 includes a first guide rail located above the first pool wall and a second guide rail located above the second pool wall. The first and second guide rails are arranged in parallel.

[0045] The guide rail is 30-50cm vertically from the water surface in the seedling pool.

[0046] The movable nozzle 21 includes a first movable nozzle and a second movable nozzle.

[0047] The first movable nozzle is located on the first guide rail and can move along the first guide rail. The first movable nozzle swings within a specific angle.

[0048] The second movable nozzle is located on the second guide rail and can move along the second guide rail. The second movable nozzle swings within a specific angle.

[0049] The movable nozzle 21 is 20-30cm away from the water surface in the seedling pond 1, and the movable nozzle 21 can swing up and down 15°.

[0050] The reciprocating mobile spray seedling system 2 performs unidirectional spraying. The mobile nozzle 21 starts spraying when moving from one end to the other and stops spraying when returning, completing one round-trip seedling cycle. After an interval of 15-20 minutes, the next round-trip seedling is carried out. During spraying, the mobile nozzle 21 swings up and down at a frequency of 10-20 times / minute. The spray axis is at an angle of 15-30° with the horizontal plane. The two mobile nozzles 21 move in the same direction and at the same speed of 1-2m / minute, covering the left and right halves of the pond respectively. The spray forms a mist curtain with an overlap area of ​​about 20cm.

[0051] The reciprocating mobile spray seedling system 2 automates spray seedling distribution, with a gentle distribution method and controllable spray area, saving labor costs while improving gamete utilization and seedling uniformity.

[0052] The shock-absorbing seedling base frame 3 includes a fixed support frame 31 and a shock-absorbing floating frame 32 located on the fixed support frame 31. The shock-absorbing floating frame 32 is connected to a waterproof micro shock-absorbing motor 33. Several shock-absorbing seedling base frames 3 are arranged on the first guide bottom 11 and the second guide bottom 12.

[0053] The fixed support frame 31 includes a column and a fixed rectangular frame, with the column fixed on the first guide bottom 11 or the second guide bottom 12.

[0054] The shock-absorbing floating frame 32 includes a floating rectangular frame, which is connected to a waterproof miniature shock-absorbing motor 33 via shock-absorbing elements.

[0055] Guide rods 342 are installed at the four corners of one of the fixed rectangular frame and the floating rectangular frame, and guide sleeves 341 are installed at the four corners of the other frame. The guide rods 342 are inserted into the guide sleeves 341.

[0056] The fixed support frame 31 is the outer frame of the shock-absorbing seedling base frame 3, and the shock-absorbing floating frame 32 is the inner frame of the shock-absorbing seedling base frame 3. The shock-absorbing seedling base frame 3 is made of stainless steel square tubing, with an epoxy resin coating to prevent seawater corrosion. The seedling curtain is fixed to the shock-absorbing floating frame 32 by suspension ropes.

[0057] The fixed support frame 31 is welded together from four columns and a fixed rectangular frame. The columns are fixed to the V-shaped guide bottom by anchor bolts 35. The fixed rectangular frame is 1.8-2m long and 0.8-1m wide, and guide sleeves 341 are installed at the four corners.

[0058] The shock-absorbing floating frame 32 is an independent floating rectangular frame with a length of 1.5-1.7m and a width of 0.6-0.8m. Guide rods 342 are installed at the four corners and are inserted into the guide sleeves 341 of the fixed support frame 31. The outer side of the long side of the shock-absorbing floating frame 32 is connected to the waterproof micro shock-absorbing motor 33 through a compression spring, so as to realize the micro-swing of the shock-absorbing floating frame 32.

[0059] The guide sleeve 341 cooperates with the guide rod 342 of the shock-absorbing floating frame 32 to control the swing direction of the shock-absorbing floating frame 32 to be vertical up and down.

[0060] The shock-absorbing seedling support frame 3 causes the seedling rope to sway slightly during the seedling collection process, increasing the probability of collision between gametophyte cells and seedling rope fibers, thus improving seedling attachment efficiency and uniformity. In the seedling cultivation stage, it can replace manual washing for cleaning the seedling curtain, reducing the washing time from 4-8 rows of sporophyte cells to 2-4 rows of cells, controlling the growth and reproduction of miscellaneous algae, and saving labor costs.

[0061] The microbubble control system 4 is located at the first guide bottom 11 at the bottom of the pool, the middle pool bottom 13, and the second guide bottom 12.

[0062] The microbubble control system 4 includes nano-aeration tubes arranged in the first guide bottom 11, the middle pool bottom 13, and the second guide bottom 12. The nano-aeration tubes generate microbubbles with a diameter of less than 50 μm.

[0063] The microbubble control system 4 generates microbubbles, and the air flotation effect keeps the gametes in a certain suspended state in the water, avoiding rapid settling and increasing the chance of seedling attachment. With the help of the V-shaped guide bottom, the rising microbubbles naturally diffuse to both sides of the pool, enhancing the air flotation effect.

[0064] The controller is configured to: install the pre-treated seedling curtain onto the shock-absorbing floating frame of the seedling base; add seawater to the seedling distribution pool to submerge the seedlings; dilute the prepared gametophyte filtrate with low-temperature seawater; control the reciprocating mobile spray seedling distribution system to spray the diluted gametophyte filtrate in one direction: the spraying is turned on when the moving nozzle goes out and turned off when it returns, completing one round-trip seedling distribution cycle; after an interval, the next round-trip seedling distribution begins; control the micro-shock motor to work intermittently: the micro-shock motor is turned on when the moving nozzle goes out and turned off when the moving nozzle returns and at the interval; control the microbubble control system to work intermittently: the microbubble control system is turned on when the moving nozzle returns and at the interval, and turned off when the moving nozzle goes out; after multiple round-trip seedling distributions, turn off the reciprocating mobile spray seedling distribution system, the microbubble control system, and the micro-shock motor, maintaining still water for a first set time to complete the seedling attachment.

[0065] This embodiment also proposes a kelp seedling cultivation method based on a microbubble circulating seedling collection device, including the following steps: Install the pre-treated seedling curtains onto the shock-absorbing floating frame attached to the seedling base; Seawater was added to the seedling tray until the water level covered the seedling curtain. The prepared gametophyte filtrate was diluted with low-temperature seawater and then sprayed onto the seedlings using a reciprocating mobile spray seedling system. The reciprocating mobile spray seedling distribution system performs unidirectional spraying: the spraying is turned on when the moving nozzle goes out and turned off when it returns, completing one round-trip seedling distribution cycle, and the next round-trip seedling distribution is carried out after an interval. The miniature damping motor operates intermittently: it turns on when the nozzle is moving forward and turns off when the nozzle is moving back and during intervals. The microbubble control system operates intermittently: it is turned on during the return stroke of the moving nozzle and at intervals, and turned off during the outward stroke of the moving nozzle. After multiple rounds of seedling placement, the reciprocating mobile spray seedling placement system, microbubble control system, and micro vibration damping motor are turned off, and the seedlings are attached in still water for the first set time.

[0066] In some embodiments, when the moving nozzles spray, the moving nozzles oscillate at a set frequency on the outward journey, and the first moving nozzle and the second moving nozzle move in the same direction and at the same speed, so that the spray overlaps.

[0067] In some embodiments, the gamete filtrate is prepared as follows: The female and male gametophytes stored at low temperature were transferred into a tissue homogenizer, sterilized ice seawater was added and chopped, and inoculated and cultured separately at a density of 1-1.5 g / L; Culture conditions: temperature 10-15℃, light 1000lx-3500lx, photoperiod L:D=12h:12h, continuous aeration for 24 hours, culture medium is sterile seawater with added NaNO3 and KH2PO4 to concentrations of 10mg / L and 2mg / L respectively, and the culture medium is changed regularly; The amplified gametophyte clones were mixed at a female to male fresh weight ratio of 2:1, placed in a tissue homogenizer, and chopped with sterile ice-cold seawater. This process was repeated 3-4 times. The mixture was then filtered until the number of cell segments visible under a microscope was 1-5 cells.

[0068] In some embodiments, the method for pre-treating the seed curtain is as follows: The seedling curtain is soaked in the mixed solution for a second set time, and air is continuously injected during soaking; The mixing ratio is: brown sugar 5-10g / L, disodium EDTA 5-10g / L, probiotics 10-20g / L; Preparation order of the mixture and activation of probiotics: First, dissolve brown sugar in a small amount of warm water, add seawater and stir well, then add probiotics, aerate and activate for 2-4 hours, add disodium EDTA, dissolve fully and then put it into the seedling tray.

[0069] The seedling curtains are soaked in a specific seawater mixture during the pretreatment stage to remove heavy metal ions and oil contaminants from the seedling ropes, and to colonize with probiotics, allowing the probiotic population to take over in advance, effectively preventing diatom proliferation and optimizing the seedling cultivation effect.

[0070] In some embodiments, seedling cultivation and management are carried out after attachment: Water temperature adjustment: 7-11℃; Water flow regulation: Control the microbubble control system to work intermittently. After the third set time for seedling attachment, apply a micro water flow with a velocity of less than 5 cm / s to the seedling pond, then turn off the microbubble control system. After the fourth set time, gradually increase the water flow to the normal seedling water flow. Light regulation: Average light intensity during gametophyte stage: 1700-1800 lx, not exceeding 2000 lx; ​​average light intensity during early sporophyte stage: 1800-2000 lx, not exceeding 3500 lx; ​​average light intensity during late sporophyte stage: 2000-2400 lx, not exceeding 7000 lx. Washing and cleaning the pond: When the sporophyte develops into 2-4 rows of cells, start washing the seedling curtain. In the early stage, clean by vibrating the micro-vibration motor. In the later stage, gradually increase the vibration intensity of the motor. If necessary, use vibration in conjunction with washing to clean. Start cleaning the pond after the fifth set time. Kelp seedlings are ready for shipment when they reach a length of 1-1.2 cm.

[0071] In the cultivation and management of seedlings, water flow and light are managed in a stepped steady state. The water flow gradually transitions from still water (24 hours after seedling attachment), microbubbles (24-72 hours after seedling attachment), and micro-flow (three days after seedling attachment) to normal flowing water (five days after seedling attachment). The light gradually transitions from low light (gametophyte), high light (early stage of young sporophyte), to strong light (late stage of young sporophyte).

[0072] The seedling cultivation and management process uses the vibration of a micro-vibration motor to replace manual washing of the seedling curtain. The intensity is controllable, which solves the problems of seedling drop and seedling detachment caused by the fragility of the young sporophytes in the early stage of rooting and the inability to remove impurities and algae through conventional seedling curtain washing. It is also easy to operate and saves labor costs.

[0073] The following is a detailed explanation of the kelp seedling cultivation method: (1) Gamete amplification and filtrate preparation The female and male gametophytes stored at low temperature were transferred into a tissue homogenizer, sterilized ice seawater was added and chopped, and then inoculated into Erlenmeyer flasks at a density of 1-1.5 g / L for culture.

[0074] Culture conditions: temperature 10-15℃, light 1000lx-3500lx, photoperiod L:D=12h:12h, continuous aeration for 24 hours, culture medium is sterile seawater with added NaNO3 and KH2PO4 to concentrations of 10mg / L and 2mg / L respectively, and the culture medium is changed once a week.

[0075] Mix the amplified gametophyte clones at a female to male fresh weight ratio of 2:1, place them in a tissue homogenizer, add sterile ice-cold seawater and chop them, repeat 3-4 times, filter through a 100-mesh sieve, and the cell segments filtered out under a microscope should ideally be 1-5 cells.

[0076] (2) Pretreatment of seedling curtains The seedling curtains are soaked in a mixture of disodium EDTA-2Na, Bacillus subtilis (a compound probiotic), brown sugar, and seawater to remove heavy metal ions and grease from the surface of the seedling ropes. This process establishes a probiotic community, prevents excessive proliferation of diatoms and other algae after seedling collection, and compresses the space for gametophytes and sporophytes to attach. The probiotic community forms a sticky biofilm on the surface of the seedling ropes, which helps the gametophytes attach to the seedlings.

[0077] Mixture ratio: brown sugar 5-10g / L, EDTA-2Na 5-10g / L, Bacillus subtilis 10-20g / L.

[0078] Preparation order of the mixture and activation of probiotics: First, dissolve brown sugar in a small amount of warm water, add seawater and stir well, then add Bacillus subtilis, aerate and activate for 2-4 hours, add EDTA-2Na, dissolve fully and then put it into the seedling tray.

[0079] Soaking time is 48-72 hours. During soaking, continuous aeration is provided to prevent Bacillus subtilis from sinking to the bottom and dying due to lack of oxygen.

[0080] (3) Attached seedlings Add seawater to the seedling pool, with the water level 6-8cm above the seedling curtain. Turn on the microbubble control system and the micro vibration damping motor. Dilute the gamete filtrate obtained in (1) with seawater at a low temperature of 5-6℃ and spray the seedlings using a reciprocating mobile spray seedling system. The seedling system uses unidirectional spraying. The spraying is turned on when the nozzle moves from one end to the other and turned off when it returns, completing one round-trip seedling cycle. The next round-trip seedling is carried out after an interval of 15-20 minutes. During spraying, the nozzle swings up and down at a frequency of 10-20 times / minute. The spray axis is at an angle of 15-30° with the horizontal plane. The two nozzles move in the same direction and at the same speed of 1-2m / minute, covering the left and right halves of the pool respectively. The spray forms a mist curtain with an overlap area of ​​about 20cm.

[0081] The micro-vibration motor works intermittently, turning on when the nozzle moves to spray, and vibrating at an extremely low frequency of 20-30 times / minute with an amplitude of 1.5-2cm, causing the seedling rope to sway slightly, increasing the chance of collision between gametophyte cells and seedling rope fibers, thus improving seedling attachment efficiency and uniformity. It turns off when the nozzle returns and during the interval. The microbubble control system operates intermittently, opening during the nozzle return journey and intervals. As the microbubbles rise, they generate an air flotation effect, keeping the gametophytes in a certain suspended state, preventing rapid settling, extending the contact time with the seedling rope, and increasing the chance of seedling attachment. The system closes when the nozzle moves to spray, preventing the microbubbles from disturbing the droplets and causing them to settle. During the seedling attachment process, the seedlings are spread back and forth 4-6 times to ensure effective coverage. After the seedling attachment is completed, the reciprocating mobile spray seedling system, microbubble control system and micro vibration damping motor are turned off, and the water is kept still for 24 hours to allow the gametophytes to be fully attached.

[0082] (4) Seedling cultivation and management Water temperature adjustment: 7-11℃.

[0083] Water flow regulation in the seedling pond: After 24 hours of seedling attachment, turn on the microbubble control system in still water, in intermittent working mode, turn on for 10 minutes and stop for 30 minutes. After 72 hours of seedling attachment, turn on the microbubble control system with a flow rate of less than 5 cm / s. After 5 days, gradually increase the water flow to the level of conventional seedling cultivation, with a flow rate of 5-20 cm / s.

[0084] Light regulation: The average light intensity during the gametophyte stage is 1700-1800 lx, not exceeding 2000 lx; ​​the average light intensity during the early stage of the sporophyte (before 0.5 mm) is 1800-2000 lx, not exceeding 3500 lx; ​​and the average light intensity during the late stage of the sporophyte (after 0.5 mm) is 2000-2400 lx, not exceeding 7000 lx.

[0085] Washing and pond cleaning: When the sporophyte develops into 2-4 rows of cells, start washing the seedling curtain. In the early stage, clean by vibrating with a micro-vibration motor to shake off the loose diatoms and sediments attached to the seedling curtain. In the later stage, gradually increase the vibration intensity of the micro-vibration motor. If necessary, use vibration pretreatment in conjunction with manual washing for cleaning. Start pond cleaning 20 days after seedling collection.

[0086] (5) Outbound Kelp seedlings are ready for shipment when they reach a length of 1-1.2 cm.

[0087] This embodiment has the following beneficial effects: (1) To solve the problem of unstable seedling attachment caused by rapid settling of gametophytes in traditional processes and improve seedling uniformity: A mobile spray seedling system is adopted, in which droplets are evenly sprayed on the water surface and fully mixed with the water during the fall process. Combined with the microbubble control system set at the bottom of the pool, the air flotation effect keeps the gametophytes suspended in the water, effectively prolonging the settling time. The low-frequency vibration of the seedling base causes the seedling rope to swing slightly, increasing the probability of collision between gametophyte cells and seedling rope fibers. The comprehensive application of the above technical means can significantly improve the seedling attachment efficiency and seedling uniformity, and solve the problems of missing seedlings and seedling drop caused by rapid settling of gametophytes.

[0088] (2) Precisely control seedling density and improve gamete utilization: By adjusting the parameters of the mobile spray seedling distribution system (spraying time, moving speed, number of reciprocating strokes), the seedling density can be accurately distributed on demand. Compared with the traditional sprinkling method, it can save the amount of gamete and reduce the cost of raw materials.

[0089] (3) By using probiotic colonization and gentle vibration to clean the seedling curtain, we can solve the problems of seedling drop and seedling fall caused by the fragility of the young sporophyte in the early stage of rooting and the inability to remove the miscellaneous algae by conventional seedling curtain washing: Before the seedling is collected, the seedling curtain is soaked in a mixture of EDTA-2Na, Bacillus subtilis, brown sugar and seawater to colonize the probiotics and allow the probiotic group to occupy the position in advance, inhibiting the attachment of miscellaneous algae from the source. After the seedling is collected, the seedling curtain is replaced by a motor-driven gentle vibration method to replace manual washing of the seedling curtain during the vulnerable period of the young sporophyte. The force is controllable, and the loose diatoms and sediments attached to the seedling curtain are shaken off in a gentler way to remove miscellaneous algae and improve the seedling effect. A certain degree of vibration can also train the seedling's resistance to stress and enable it to attach and grow better.

[0090] (4) Save labor costs and reduce operational intensity: The mobile spray seedling system, the shock absorption system and the microbubble control system are all automatically controlled by the controller. Parameters such as nozzle movement speed, spray pressure, spray angle, interval duration, shock absorption frequency, shock absorption interval and microbubble generation rate can be quantitatively set, which reduces the reliance on the technician's experience. At the same time, it reduces the manual washing of the seedling curtain, which can greatly save labor costs and reduce the labor intensity of operators.

[0091] In summary, this embodiment has outstanding advantages such as stable seedling attachment, low seedling detachment rate, light algal contamination, and simple operation, providing a modern technical solution for the kelp seedling industry that is efficient, reliable, and easy to scale up.

[0092] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit them. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions claimed by the present invention.

Claims

1. A microbubble circulating seedling collection device, characterized in that, The device includes: The seedling pool includes a pool bottom, a first pool wall and a second pool wall, and the pool bottom includes a central pool bottom, a first guide bottom that gradually increases in height from the central pool bottom toward the first pool wall, and a second guide bottom that gradually increases in height from the central pool bottom toward the second pool wall. A reciprocating mobile spraying seedling system, including guide rails and mobile nozzles; The shock-absorbing seedling base frame includes a fixed support frame and a shock-absorbing floating frame located on the fixed support frame. The shock-absorbing floating frame is connected to a waterproof micro shock-absorbing motor. Several shock-absorbing seedling base frames are arranged on the first and second guide bottoms. The microbubble control system is located at the first guide bottom, the middle pool bottom, and the second guide bottom; The controller is configured to control the reciprocating mobile spray seedling system to spray the diluted gamete filtrate in one direction: the spraying is turned on when the mobile nozzle goes out and turned off when it returns, completing one round-trip seedling cycle, and then the next round-trip seedling is performed after an interval; the controller controls the micro-vibration motor to work intermittently: the micro-vibration motor is turned on when the mobile nozzle goes out and turned off when the mobile nozzle returns and during the interval; the controller controls the microbubble control system to work intermittently: the microbubble control system is turned on when the mobile nozzle returns and during the interval, and turned off when the mobile nozzle goes out; after multiple round-trip seedlings, the reciprocating mobile spray seedling system, the microbubble control system, and the micro-vibration motor are turned off, and the seedlings are attached while maintaining still water for a first set time.

2. The microbubble circulating seedling collection device according to claim 1, characterized in that, The extension direction of the central pool bottom is the same as the extension direction of the first pool wall and the second pool wall, and the inclination of the first guide bottom and the second guide bottom is 5-8°.

3. The microbubble circulating seedling collection device according to claim 1, characterized in that, The guide rail includes: A first guide rail located above the first pool wall and a second guide rail located above the second pool wall; The movable nozzle includes: A first movable nozzle is located on the first guide rail and can move along the first guide rail. The first movable nozzle is configured to swing within a specific angle. A second movable nozzle is located on and movable along the second guide rail, and the second movable nozzle is configured to oscillate within a specific angle.

4. The microbubble circulating seedling collection device according to claim 1, characterized in that, The fixed support frame includes a column and a fixed rectangular frame, and the column is fixed on the first guide bottom or the second guide bottom; The shock-absorbing floating frame includes a floating rectangular frame, which is connected to a waterproof miniature shock-absorbing motor via shock-absorbing elements. A guide rod is installed on one of the fixed rectangular frame and the floating rectangular frame, and a guide sleeve is installed on the other. The guide rod is inserted into the guide sleeve.

5. The microbubble circulating seedling collection device according to claim 1, characterized in that, The microbubble control system includes nano-aeration tubes arranged at the first guide bottom, the middle pool bottom, and the second guide bottom, which generate microbubbles with a diameter of less than 50 μm.

6. A method for cultivating kelp seedlings based on the microbubble circulating seedling collection device according to any one of claims 1-5, characterized in that, Includes the following steps: Install the pretreated seedling curtain onto the shock-absorbing floating frame of the seedling base frame; Seawater is added to the seedling pool until the water level covers the seedling curtain. The prepared gametophyte filtrate is diluted with low-temperature seawater and then sprayed onto the seedlings using the reciprocating mobile spray seedling system. The reciprocating mobile spray seedling system performs unidirectional spraying: the mobile nozzle turns on spraying when going out and turns off spraying when returning, completing one round-trip seedling distribution cycle, and then performs the next round-trip seedling distribution after an interval. The micro vibration damping motor operates intermittently: it turns on when the moving nozzle is going out, and turns off when the moving nozzle is returning and at intervals. The microbubble control system operates intermittently: it is turned on when the moving nozzle returns and at intervals, and turned off when the moving nozzle goes out. After multiple rounds of seedling placement, the reciprocating mobile spray seedling placement system, microbubble control system, and micro vibration damping motor are turned off, and the seedlings are attached in still water for the first set time.

7. The kelp seedling cultivation method according to claim 6, characterized in that, When the moving nozzle sprays, it oscillates at a set frequency on its outward journey, and the first and second moving nozzles move in the same direction and at the same speed, so that the spray overlaps.

8. The kelp seedling cultivation method according to claim 6, characterized in that, The method for preparing the gamete filtrate is as follows: The female and male gametophytes stored at low temperature were separately added to sterilized ice seawater, chopped up, and inoculated and cultured at a density of 1-1.5 g / L. Culture conditions: temperature 10-15℃, light 1000lx-3500lx, photoperiod L:D=12h:12h, continuous aeration for 24 hours, culture medium is sterile seawater with added NaNO3 and KH2PO4 to concentrations of 10mg / L and 2mg / L respectively, and the culture medium is changed regularly; The amplified gametophyte clones were mixed at a female to male fresh weight ratio of 2:1, sterilized ice seawater was added and chopped, and the mixture was repeated several times. The mixture was then filtered until the cell segments filtered out under a microscope were 1-5 cells.

9. The kelp seedling cultivation method according to claim 6, characterized in that, The method for pre-treating the seedling curtain is as follows: The seedling curtain is soaked in the mixture for a second set time, and air is continuously injected during soaking; The mixture ratio is: brown sugar 5-10g / L, disodium EDTA 5-10g / L, probiotics 10-20g / L; Preparation order of the mixture and activation of probiotics: First, dissolve brown sugar in a small amount of warm water, add seawater and stir well, then add probiotics, aerate and activate for 2-4 hours, add disodium EDTA, dissolve fully and then put it into the seedling tray.

10. The kelp seedling cultivation method according to claim 6, characterized in that, Seedling cultivation and management follow the attachment process: Water temperature adjustment: 7-11℃; Water flow regulation: Control the microbubble control system to work intermittently. After the third set time of seedling attachment, apply a micro water flow with a velocity of less than 5 cm / s to the seedling pond, turn off the microbubble control system, and after the fourth set time, gradually increase the water flow to the normal seedling water flow. Light regulation: Average light intensity during gametophyte stage: 1700-1800 lx, not exceeding 2000 lx; ​​average light intensity during early sporophyte stage: 1800-2000 lx, not exceeding 3500 lx; ​​average light intensity during late sporophyte stage: 2000-2400 lx, not exceeding 7000 lx. Washing and cleaning the pond: When the sporophyte develops into 2-4 rows of cells, start washing the seedling curtain. In the early stage, clean by vibrating a micro-vibration motor. In the later stage, gradually increase the vibration intensity of the motor. Start cleaning the pond after the fifth set time. Kelp seedlings are ready for shipment when they reach a length of 1-1.2 cm.

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