Construction method and application of congenital long bone short mouse model

The ANKS3 gene knockout mouse model was constructed using the Cre-loxP and Prx1-Cre systems, which solved the problem of the lack of long-bone short mouse models, enabled disease phenotype simulation and drug development, and provided disease regulation and diagnostic tools.

CN122397686APending Publication Date: 2026-07-17THE OBSTETRICS & GYNECOLOGY HOSPITAL OF FUDAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
THE OBSTETRICS & GYNECOLOGY HOSPITAL OF FUDAN UNIV
Filing Date
2026-04-23
Publication Date
2026-07-17

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Abstract

本发明公开了一种先天性长骨短小鼠模型的构建方法，包括以下步骤：将F0代Anks3‑flox精子与野生型小鼠卵细胞体外受精后，把胚胎移植入假孕母鼠中，获得F1代Anks3‑flox杂合小鼠；将F1代Anks3‑flox杂合雌鼠与Anks3‑flox杂合雄鼠配繁，获得F2代Anks3‑flox纯合小鼠；将F2代Anks3‑flox纯合小鼠与Prx1‑cre小鼠配繁，获得F3代Anks3‑flox杂合且Prx1‑cre小鼠；将F3代Anks3‑flox杂合且Prx1‑cre小鼠与F2代Anks3‑flox纯合小鼠配繁，获得F4代Anks3‑flox纯合且Prx1‑cre小鼠，即为先天性长骨短小鼠模型。本发明的构建方法，简单高效，制得的先天性长骨短小鼠模型，为长骨短疾病的临床治疗提供新的治疗靶点，也为筛选预防或治疗长骨短表型遗传性疾病的药物提供很好的动物模型，具有广阔的应用前景。
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