A traditional Chinese medicine prescription for preventing and treating blue-ear disease, a preparation method and application thereof

Abstract

This invention discloses a traditional Chinese medicine formula for the prevention and treatment of porcine reproductive and respiratory syndrome (PRRS), its preparation method, and its application, belonging to the field of traditional Chinese veterinary medicine technology. The formula, by weight, comprises: 400-500 parts of Anemarrhena asphodeloides, 300-450 parts of Scutellaria baicalensis, 200-350 parts of Forsythia suspensa, 150-300 parts of Trichosanthes kirilowii, 150-300 parts of Lonicera japonica, 300-450 parts of Cornus officinalis, 300-450 parts of Eucommia ulmoides, 150-300 parts of Pueraria lobata, 150-300 parts of Polygonatum sibiricum, 150-300 parts of Rehmannia glutinosa, 200-400 parts of Poria cocos, 200-400 parts of Citrus reticulata peel, 200-400 parts of Platycodon grandiflorus, 150-300 parts of Bupleurum chinense, and 200-350 parts of Glycyrrhiza uralensis. The preparation method includes: high-temperature alcohol extraction of the raw materials, filtration and concentration, and spray drying to obtain a powdered extract of the traditional Chinese medicine. This invention is based on the treatment principles of clearing heat and drying dampness, harmonizing the lesser yang, and strengthening the spleen and resolving phlegm. In the formula, Bupleurum harmonizes the lesser yang to allow the pathogenic factors to exit, while Poria, Citrus reticulata peel, and Platycodon grandiflorus strengthen the spleen and resolve phlegm to treat secondary infections. The entire formula is highly specific to the porcine reproductive and respiratory syndrome virus. Clinical studies have shown that adding 0.3%-0.5% to feed can clear viremia in 3-5 weeks, significantly reducing morbidity and mortality, and leaving no drug residues, making it suitable for industrial production.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine veterinary drugs, and further to a traditional Chinese medicine formula for the prevention and treatment of porcine reproductive and respiratory syndrome (PRRS), its preparation method, and its application. Background Technology

[0002] Porcine reproductive and respiratory syndrome (PRRS) is a highly contagious disease caused by the PRRS virus, leading to reproductive disorders in breeding pigs and respiratory symptoms in pigs of all ages, causing huge economic losses to the global pig industry. Current prevention and control methods mainly rely on vaccination and antibiotics to control secondary infections. However, due to frequent viral mutations and significant immunosuppression issues, the protective effect of existing vaccines is not ideal, while antibiotics are ineffective against the virus and easily lead to residues and drug resistance. Traditional Chinese medicine (TCM) has unique advantages in the prevention and treatment of viral diseases, but existing TCM preparations for PRRS often suffer from problems such as complex composition, unclear targets, low absorption rate of active ingredients, and slow onset of action, resulting in unstable clinical efficacy, particularly failing to effectively eliminate the virus and achieve antigen seroconversion.

[0003] Therefore, there is an urgent need to develop a traditional Chinese medicine formula with a clear formulation, a well-defined mechanism of action, easily absorbed active ingredients, that can effectively prevent and treat porcine reproductive and respiratory syndrome (PRRS) and promote viral clearance. Summary of the Invention

[0004] In view of the above-mentioned technical problems, the purpose of this invention is to provide a traditional Chinese medicine formula for the prevention and treatment of porcine reproductive and respiratory syndrome (PRRS), which can solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution: This application provides a traditional Chinese medicine formula for the prevention and treatment of porcine reproductive and respiratory syndrome (PRRS), comprising the following ingredients by weight: Anemarrhena asphodeloides 400-500 parts, Scutellaria baicalensis 300-450 parts, Forsythia suspensa 200-350 parts, Trichosanthes kirilowii 150-300 parts, Lonicera japonica 150-300 parts, Cornus officinalis 300-450 parts, Eucommia ulmoides 300-450 parts, Pueraria lobata 150-300 parts, Polygonatum sibiricum 150-300 parts, Rehmannia glutinosa 150-300 parts, Poria cocos 200-400 parts, Citrus reticulata 200-400 parts, Platycodon grandiflorus 200-400 parts, Bupleurum chinense 150-300 parts, Glycyrrhiza uralensis 200-350 parts.

[0006] In some embodiments, the ingredients, by weight, include the following: Anemarrhena asphodeloides 450 parts, Scutellaria baicalensis 380 parts, Forsythia suspensa 280 parts, Trichosanthes kirilowii 220 parts, Lonicera japonica 220 parts, Cornus officinalis 380 parts, Eucommia ulmoides 380 parts, Pueraria lobata 220 parts, Polygonatum sibiricum 220 parts, Rehmannia glutinosa 220 parts, Poria cocos 300 parts, Citrus reticulata 300 parts, Platycodon grandiflorus 300 parts, Bupleurum chinense 220 parts, Glycyrrhiza uralensis 280 parts.

[0007] According to another aspect of the present invention, the present invention further provides a method for preparing a traditional Chinese medicine formula for preventing and treating porcine reproductive and respiratory syndrome (PRRS), comprising the following steps: S1: Weigh each raw material according to the pre-weight parts, and use high-temperature alcohol extraction or water extraction of Chinese herbal medicine slices to obtain the extract of each single herb. S2: Combine and mix the extracts of the above-mentioned single herbs according to the formula ratio, and then filter. S3: Concentrate the filtered compound extract to a clear paste with a relative density of 1.05-1.15, and then spray dry to obtain the Chinese herbal extract powder.

[0008] In some embodiments, during step S1, a solvent of 6-10 times the total weight of the medicinal materials is added during the high-temperature alcohol extraction or water extraction process, and the extraction is performed 1-3 times, each time for 1-2 hours, and the extracts are combined.

[0009] In some embodiments, the high-temperature alcohol extraction in step S1 is performed twice. The first time, 8-10 times the total weight of the medicinal materials is added to the ethanol and the mixture is decocted for 1.5-2 hours. The second time, 6-8 times the total weight of the medicinal materials is added to the ethanol and the mixture is decocted for 1-1.5 hours.

[0010] In some embodiments, the inlet air temperature of the spray drying in step S3 is 160-200°C, and the outlet air temperature is 80-100°C.

[0011] According to another aspect of the present invention, the present invention further provides the use of the traditional Chinese medicine formula for preventing and treating porcine reproductive and respiratory syndrome (PRRS) according to the above embodiments or the traditional Chinese medicine extract prepared by any of the above methods in the preparation of drugs or feed additives for preventing and treating PRRS.

[0012] In some embodiments, the drug or feed additive is administered orally by mixing with feed; when used for treatment, it is added at a ratio of 0.3%-0.5% of the total feed weight and used for 5-7 consecutive days; when used for prevention, it is added at a ratio of 0.3%-0.5% of the total feed weight and used for 3-5 consecutive weeks.

[0013] Compared with the prior art, the traditional Chinese medicine formula for the prevention and treatment of porcine reproductive and respiratory syndrome (PRRS) provided by this invention has at least the following beneficial effects: 1. This invention, from the perspective of TCM disease diagnosis and treatment, classifies porcine reproductive and respiratory syndrome (PRRS) as heat syndrome, excess syndrome, interior syndrome, and yang syndrome. The treatment principle is to "clear heat and dry dampness, harmonize the lesser yang, and strengthen the spleen and resolve phlegm". This is combined with the results of screening Chinese medicines against PRRS in modern pharmacology. The formula corresponds to the syndrome and has strong targeting. At the same time, it innovatively introduces Bupleurum as a key medicine to harmonize the lesser yang. In response to the immunosuppression and persistent infection characteristics caused by PRRS, it regulates the lesser yang mechanism and allows the pathogen to have an outlet. This makes up for the shortcomings of existing formulas that only clear heat and detoxify but neglect "expelling the pathogen". 2. This invention abandons the outdated traditional process of directly pulverizing and mixing Chinese herbal medicines into feed. It employs a high-temperature alcohol extraction method, eliminating the need for pre-pulverization and directly extracting the medicinal herbs. This simplifies the process and allows for more complete dissolution of the fat-soluble active ingredients from multiple herbs such as Anemarrhena asphodeloides, Scutellaria baicalensis, Forsythia suspensa, and Bupleurum chinense, significantly improving the extraction rate and bioavailability of the active ingredients. The resulting extract, when added to feed, can achieve rapid relief of high fever and wheezing symptoms within 3 days and recovery within 5-7 days. Detailed Implementation

[0014] The endpoints and any values ​​of the ranges disclosed in this invention are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of the various ranges, the endpoint values ​​of the various ranges and individual point values, and individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed in this invention.

[0015] The present application will be further described in detail below with reference to embodiments and comparative examples, but the scope of protection of the present invention is not limited to the contents described.

[0016] Examples 1-2 and Comparative Examples 1-3 provide traditional Chinese medicine prescriptions for the prevention and treatment of porcine reproductive and respiratory syndrome (PRRS) and their preparation methods.

[0017] Example 1 A traditional Chinese medicine formula for the prevention and treatment of porcine reproductive and respiratory syndrome (PRRS) includes the following ingredients by weight: Anemarrhena asphodeloides 450 parts, Scutellaria baicalensis 380 parts, Forsythia suspensa 280 parts, Trichosanthes kirilowii 220 parts, Lonicera japonica 220 parts, Cornus officinalis 380 parts, Eucommia ulmoides 380 parts, Pueraria lobata 220 parts, Polygonatum sibiricum 220 parts, Rehmannia glutinosa 220 parts, Poria cocos 300 parts, Citrus reticulata 300 parts, Platycodon grandiflorus 300 parts, Bupleurum chinense 220 parts, Glycyrrhiza uralensis 280 parts.

[0018] Preparation method: S1: Weigh each raw material according to the above-mentioned weight proportions and mix them evenly; S2: Add 70% ethanol (8 times the total weight of the herbs) to the mixed herbs and reflux extract twice at 75°C. The first reflux extraction is for 1.5 hours, and the second extraction is for 1 hour with 70% ethanol (6 times the total weight of the herbs). Combine the extracts and filter. S3: Concentrate the filtered decoction to a clear paste with a relative density of 1.10, and then spray dry it (inlet air temperature 180℃, outlet air temperature 90℃) to obtain a powder of traditional Chinese medicine extract.

[0019] Example 2 A traditional Chinese medicine formula for the prevention and treatment of porcine reproductive and respiratory syndrome (PRRS) includes the following ingredients by weight: Anemarrhena asphodeloides 470 parts, Scutellaria baicalensis 420 parts, Forsythia suspensa 320 parts, Trichosanthes kirilowii 270 parts, Lonicera japonica 270 parts, Cornus officinalis 420 parts, Eucommia ulmoides 420 parts, Pueraria lobata 270 parts, Polygonatum sibiricum 270 parts, Rehmannia glutinosa 270 parts, Poria cocos 350 parts, Citrus reticulata 350 parts, Platycodon grandiflorus 350 parts, Bupleurum chinense 270 parts, Glycyrrhiza uralensis 320 parts.

[0020] Preparation method: S1: Weigh each raw material according to the above weight proportions, grind to 40 mesh, and mix evenly; S2: Add 10 times the total weight of the medicinal materials to the mixed medicinal materials with 80% ethanol by volume, and reflux extract twice at 80℃. The first reflux extraction is for 2 hours, and the second extraction is for 1.5 hours with 8 times the total weight of the medicinal materials added. Combine the extracts and filter. S3: Concentrate the filtered decoction to a clear paste with a relative density of 1.15, and then spray dry it (inlet air temperature 200℃, outlet air temperature 100℃) to obtain a powder of traditional Chinese medicine extract.

[0021] Comparative Example 1 The only difference from Example 1 is that Anemarrhena asphodeloides and Scutellaria baicalensis are removed, and an equal amount of gypsum and Coptis chinensis are added. The rest of the formulation and preparation method are the same as in Example 1.

[0022] Comparative Example 2 The difference from Example 1 is that Bupleurum, Tangerine Peel and Poria are removed, while the rest of the formula and preparation method are the same as in Example 1.

[0023] Comparative Example 3 The difference between this comparative example and Example 1 is that the formulation is exactly the same as that of Example 1, but the high-temperature alcohol extraction process is not used. Instead, each raw material is directly pulverized and passed through a 60-mesh sieve and mixed evenly to obtain a traditional Chinese medicine powder.

[0024] In vitro anti-PRRS virus experiment Drug preparation: The dried Chinese medicine powders obtained in Examples 1-3 and Comparative Examples 1-3 were prepared into test solutions using serum-free culture medium.

[0025] Virus: Porcine reproductive and respiratory syndrome virus (PRRSV) virulent strain; Cells: Marc-145 cells.

[0026] Experimental methods: The cytopathic effect (CPE) inhibition method was used to evaluate the inhibitory effect of the traditional Chinese medicine extracts of Examples 1-2 and Comparative Examples 1-3 on PRRSV. In the drug test group, different concentrations of traditional Chinese medicine test solution (10-fold, 100-fold, 200-fold, 400-fold, and 800-fold dilution) were mixed with an equal volume of virus solution (100 TCID50), incubated, and then inoculated into cells. After incubation at room temperature for 30 minutes, the mixture was added to cell culture plates. After culturing for 5 days, cytopathic effect (CPE) was observed, and the half-maximal inhibitory concentration (IC50) and maximum non-toxic concentration (TC0) were calculated. Finally, the inhibition rate was calculated, and the results are shown in Table 1.

[0027]

[0028] Table 1 The results showed that the inhibition rates of Examples 1 and 2 were significantly stronger than those of Comparative Examples 1-3, and they still maintained an inhibition rate of over 80% even after 400-fold dilution. The inhibition rates of Comparative Examples 1 and 2 decreased significantly, indicating that the combination of Anemarrhena asphodeloides-Scutellaria baicalensis and the combination of Bupleurum chinense-Citrus reticulata-Poria cocos were crucial to the antiviral effect of this formula and none of them could be omitted. The inhibition rate of Comparative Example 3 was extremely low, indicating that the antiviral effect of the directly pulverized powder was extremely poor because the effective components were not dissolved.

[0029] Clinical treatment trial Pig farms where porcine reproductive and respiratory syndrome (PRRS) has been selected, and piglets around 50 days old that show clinical symptoms such as fever, cough, wheezing, and purple ears are randomly divided into 7 groups.

[0030] Examples 1-2: The animals were fed feed containing 0.4% of the herbal extracts from Examples 1-2 for 7 consecutive days.

[0031] Comparative groups 1-3: Feeds containing 0.4% of the traditional Chinese medicine product from comparative groups 1-3 were given for 7 consecutive days.

[0032] Control group 1: Feeded with a therapeutic dose of tilmicosin for 7 consecutive days. The results are shown in Table 2 for thermodynamic performance evaluation.

[0033]

[0034] Table 2 The comparative experimental results show that, as can be seen from the data in Table 2 above, under the same production conditions, the survival rate of piglets treated with the traditional Chinese medicine of this invention starting on the 9th day after the onset of porcine reproductive and respiratory syndrome (PRRS) at around 50 days of age was 91.87% (Example 1); the survival rate of piglets treated with the antibiotic tilmicosin was 84.30% (Comparative Example 1). This demonstrates that the traditional Chinese medicine product of this invention has a significant effect in the treatment of PRRS.

[0035] Prevention efficacy trial Healthy 30-day-old piglets were selected and randomly divided into groups of 30 each. The prevention group was fed with feed containing the herbal extract from Example 1 (0.3%) for 3 consecutive weeks. The control group was fed with a basic feed. After the feeding was completed, the piglets were challenged with a virulent strain of porcine reproductive and respiratory syndrome (PRRS) (nasal drops + intramuscular injection). The morbidity and mortality rates were observed within 21 days. The results are shown in Table 3.

[0036]

[0037] Table 3 The results showed that in Example 1, the incidence rate after challenge was only 10%, the mortality rate was 3.3%, and the protection rate was as high as 96.7%; while the protection rates of Comparative Examples 1 and 3 were significantly lower, and the mortality rate of the blank control group was as high as 40%. This indicates that the traditional Chinese medicine formula of the present invention has excellent preventive effect against porcine reproductive and respiratory syndrome (PRRS).

[0038] In summary, the traditional Chinese medicine formula and its preparation method for preventing and treating porcine reproductive and respiratory syndrome (PRRS) provided by this invention have a wide range of raw material sources, mature preparation technology, and are suitable for industrial mass production. The product can be used as a feed additive or drinking water agent, is convenient to administer, has a rapid onset of action, leaves no drug residues, meets the requirements of green farming and food safety, and has broad market application prospects.

[0039] The above are merely preferred embodiments of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A traditional Chinese medicine formula for the prevention and treatment of porcine reproductive and respiratory syndrome (PRRS), characterized in that, By weight, it includes the following ingredients: Anemarrhena asphodeloides 400-500 parts, Scutellaria baicalensis 300-450 parts, Forsythia suspensa 200-350 parts, Trichosanthes kirilowii 150-300 parts, Lonicera japonica 150-300 parts, Cornus officinalis 300-450 parts, Eucommia ulmoides 300-450 parts, Pueraria lobata 150-300 parts, Polygonatum sibiricum 150-300 parts, Rehmannia glutinosa 150-300 parts, Poria cocos 200-400 parts, Citrus reticulata 200-400 parts, Platycodon grandiflorus 200-400 parts, Bupleurum chinense 150-300 parts, Glycyrrhiza uralensis 200-350 parts.

2. The traditional Chinese medicine formula for preventing and treating porcine reproductive and respiratory syndrome (PRRS) according to claim 1, characterized in that, By weight, it includes the following ingredients: Anemarrhena asphodeloides 450 parts, Scutellaria baicalensis 380 parts, Forsythia suspensa 280 parts, Trichosanthes kirilowii 220 parts, Lonicera japonica 220 parts, Cornus officinalis 380 parts, Eucommia ulmoides 380 parts, Pueraria lobata 220 parts, Polygonatum sibiricum 220 parts, Rehmannia glutinosa 220 parts, Poria cocos 300 parts, Citrus reticulata 300 parts, Platycodon grandiflorus 300 parts, Bupleurum chinense 220 parts, Glycyrrhiza uralensis 280 parts.

3. A method for preparing a traditional Chinese medicine formula for the prevention and treatment of porcine reproductive and respiratory syndrome (PRRS), characterized in that, Includes the following steps: S1: Weigh each raw material according to the pre-weight parts, and use high-temperature alcohol extraction or water extraction of Chinese herbal medicine slices to obtain the extract of each single herb. S2: Combine and mix the extracts of the above-mentioned single herbs according to the formula ratio, and then filter. S3: Concentrate the filtered compound extract to a clear paste with a relative density of 1.05-1.15, and then spray dry to obtain the Chinese herbal extract powder.

4. The method for preparing the traditional Chinese medicine formula for preventing and treating porcine reproductive and respiratory syndrome (PRRS) according to claim 3, characterized in that, In step S1, during the high-temperature alcohol extraction or water extraction process, add a solvent of 6-10 times the total weight of the medicinal materials, extract 1-3 times, each time for 1-2 hours, and combine the extracts.

5. The method for preparing the traditional Chinese medicine formula for preventing and treating porcine reproductive and respiratory syndrome (PRRS) according to claim 3, characterized in that, In step S1, the high-temperature alcohol extraction is performed twice. The first time, 8-10 times the total weight of the medicinal materials is added to the ethanol and the mixture is decocted for 1.5-2 hours. The second time, 6-8 times the total weight of the medicinal materials is added to the ethanol and the mixture is decocted for 1-1.5 hours.

6. The method for preparing the traditional Chinese medicine formula for preventing and treating porcine reproductive and respiratory syndrome (PRRS) according to claim 3, characterized in that, In step S3, the inlet air temperature for spray drying is 160-200℃, and the outlet air temperature is 80-100℃.

7. The use of a traditional Chinese medicine formula as described in claim 1 or 2, or a traditional Chinese medicine extract prepared by any of the methods in claims 3-6, in the preparation of a drug or feed additive for the prevention and treatment of porcine reproductive and respiratory syndrome (PRRS).

8. The application according to claim 7, characterized in that, The medication or feed additive is administered orally by mixing it into the feed. When used for treatment, it is added at a ratio of 0.3%-0.5% of the total feed weight and used continuously for 5-7 days. When used for prevention, it is added at a ratio of 0.3%-0.5% of the total feed weight and used continuously for 3-5 weeks.

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