An apparatus for simulating biodegradation of nitrosamines and a test method thereof

By constructing a microcosm reaction system, the biodegradation process of nitrosamines in the groundwater environment was realistically simulated, which solved the problem of insufficient simulation in the existing technology and realized accurate monitoring of the degradation behavior and kinetic characteristics of nitrosamines and the revelation of the microbial action mechanism.

CN122405404APending Publication Date: 2026-07-17GUILIN UNIVERSITY OF TECHNOLOGY
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Patent Information

Application Number
CN202610460631.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-04-09
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing technologies cannot realistically simulate the biodegradation process of nitrosamines in groundwater environments, especially under conditions of low nutrition, low flow rate, weak disturbance, and synergistic effects of natural microorganisms. Furthermore, it is difficult to dynamically and continuously monitor nitrosamines and their degradation products, leading to deviations between experimental results and actual environmental conditions.

Method used

Using a microcosmic reaction system, actual groundwater samples and soil matrix were used to set up aerobic and anaerobic conditions. Combined with high and low pollution water and soil, nutrients were added, and dynamic continuous monitoring of nitrosamines and degradation products was carried out. The microbial community was analyzed by 16S rDNA and the decay half-life was calculated.

Benefits of technology

This study achieved a realistic simulation of the biodegradation behavior of nitrosamines, improved the reliability and repeatability of experimental results, revealed the degradation kinetics and microbial action mechanism, and provided technical support for groundwater pollution remediation.

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Abstract

本发明公开了一种模拟亚硝胺生物降解的装置及其试验方法,属于水环境污染控制与地下水修复技术领域。所述装置为微宇宙反应单元,所述试验方法通过采集不同污染程度地下水及对应土壤基质,构建好氧和厌氧微宇宙体系,向体系中加入NDMA、NDEA、NPYR和NDBA混合标准液,并通过设置土壤基质组、营养物组和空白组,结合LC‑MS / MS检测、土壤样品分析及16S rDNA测序,获取亚硝胺在不同条件下的浓度变化、半衰期、土壤保留行为、降解产物变化及优势微生物信息。本发明能够较真实地模拟地下水环境中亚硝胺的生物降解过程,可用于揭示亚硝胺在不同污染背景、氧化还原条件和营养水平下的生物降解规律及微生物作用机制。
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