Cationic lipid hybrid membrane vesicles for genetic transformation of diatoms, and methods of making and using the same
By constructing cationic lipid hybrid membrane vesicles, the problems of expensive equipment and cell damage in diatom genetic transformation were solved, achieving efficient and mild genetic transformation and improving the efficiency of diatom internalization and the accuracy of gene delivery.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- XIAMEN UNIV
- Filing Date
- 2026-04-29
- Publication Date
- 2026-07-17
AI Technical Summary
Existing methods for genetic transformation of diatoms rely on expensive equipment, are complex to operate, and cause significant cell damage. Furthermore, natural BMVs are randomly loaded, have a wide target range, and low internalization efficiency, making it difficult to efficiently and gently cross the diatom's silica shell barrier.
By ultrasonically fusing positively charged cationic liposomes with negatively charged natural BMVs, cationic-liposome hybrid membrane vesicles are constructed. Their surface charge and physicochemical properties are regulated, and combined with electroporation, exogenous plasmids are precisely loaded, improving internalization efficiency and avoiding loading randomness.
This provides an efficient, mild, and economical genetic transformation method that simplifies operations, reduces cell damage, improves transformation efficiency, and overcomes the diatom silica shell barrier, making it suitable for gene function research and metabolic engineering of diatoms.
Smart Images
Figure CN122405705A_ABST