A primer probe combination for detecting goose virus based on MALDI-TOF mass spectrometry and application thereof
By using MALDI-TOF mass spectrometry and primer-probe combination, we have achieved efficient and rapid detection of goose-derived viruses, solving the detection challenges in existing technologies and enabling simultaneous detection and low-cost diagnosis of 16 viruses.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- ZHONGKAI UNIV OF AGRI & ENG
- Filing Date
- 2026-05-08
- Publication Date
- 2026-07-17
AI Technical Summary
Existing technologies are insufficient for the rapid, quantitative, and cost-effective detection of co-infected goose viruses, especially failing to meet the needs of rapid clinical diagnosis and precise prevention and control.
Using MALDI-TOF mass spectrometry combined with specific primer and probe combinations, 16 goose-derived viruses were simultaneously detected through multiplex PCR amplification and extension reactions. The detection was automated and parallelized using a kit.
It achieves high-throughput, rapid, and low-cost detection of goose-borne viruses within 2 hours, with detection limits as low as 2.87–29 copies/μL. It can detect low viral load infections and avoid non-specific amplification, making it suitable for accurate detection of mixed infections.
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Figure CN122405899A_ABST