Serum adiponectin detection method using pedot and pss modified screen printed carbon electrode

By modifying the carbon mesh electrode with PEDOT and PSS, and combining the composite dispersion of GO and PEDOT:PSS with antibody immobilization technology, the problem of balancing conductivity and antibody immobilization ability in existing adiponectin detection was solved, achieving electrochemical detection with high sensitivity and stability.

CN122409774APending Publication Date: 2026-07-17ANHUI GUOXIN DIAGNOSTIC BIOTECHNOLOGY CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ANHUI GUOXIN DIAGNOSTIC BIOTECHNOLOGY CO LTD
Filing Date
2026-04-15
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing adiponectin detection methods suffer from problems such as long detection procedures, cumbersome operation, high cost, and high requirements for conditions. Furthermore, existing modified electrodes cannot achieve a balance between conductivity and antibody immobilization ability, resulting in insufficient detection sensitivity, stability, and repeatability.

Method used

A carbon mesh electrode was modified with PEDOT and PSS. GO and PEDOT:PSS were homogenized in DMSO at a specific mass ratio to form a composite dispersion. Electrode activation, antibody immobilization, and blocking were then performed. Combined with DPV detection, a stable electrochemical detection interface was constructed.

Benefits of technology

This improved the sensitivity, stability, and repeatability of adiponectin detection, enabling rapid and accurate quantitative detection, reducing detection costs, and enhancing the sensitivity and repeatability of the detection.

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Abstract

本发明公开了PEDOT和PSS修饰丝网碳电极血清脂联素检测方法,具体涉及脂联素检测技术领域,包括将聚3,4‑乙撑二氧噻吩‑聚苯乙烯磺酸和氧化石墨烯按设定质量比加入二甲基亚砜中均质分散,滴涂于活化后的丝网印刷碳电极表面形成复合修饰层,经活化后固定脂联素抗体并封闭未结合位点,再采用差分脉冲伏安法测量待测样本加入前后的电流变化量,以确定人血清中脂联素浓度;本发明能够兼顾电子传递能力与抗体固定能力,提高检测灵敏度、稳定性和重复性。
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