A DNA tetrahedron-based method for the dual-phase electrochemical detection of microRNA in peripheral blood of renal cancer patients
The biphasic electrochemical detection method combining DNA tetrahedral nanostructures with CRISPR/Cas12a reaction solves the problems of cumbersome probe immobilization, high reagent consumption, strong matrix interference, and insufficient sensitivity in existing technologies, achieving highly sensitive and specific miRNA quantitative detection, which is suitable for clinical sample analysis.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- THE FIRST AFFILIATED HOSPITAL OF FUJIAN MEDICAL UNIV
- Filing Date
- 2026-05-29
- Publication Date
- 2026-07-17
AI Technical Summary
Existing electrochemical nucleic acid detection technologies suffer from problems such as cumbersome probe immobilization, high reagent consumption in homogeneous systems, strong matrix interference, insufficient sensitivity, and poor clinical applicability, making it difficult to achieve high sensitivity and high specificity for the detection of circulating miRNAs.
A biphasic electrochemical detection method combining DNA tetrahedral nanostructures (TDNs) with CRISPR/Cas12a reaction is employed. Target recognition and enzyme digestion amplification are completed in the solution phase, with the electrode serving only as the signal readout interface. By utilizing the efficient trans-cleavage of CRISPR/Cas12a and the steric hindrance-gated signal enhancement of DNA tetrahedrons, probe-free immobilization, micro-scale reaction volume, and highly interference-resistant miRNA quantification are achieved.
It achieves probe-free, micro-volume, high-sensitivity, and matrix-resistant quantitative detection of miRNAs, with a detection limit as low as 4.1 aM and a linear range of 10 aM to 1 pM. It is suitable for clinical sample testing, exhibits excellent specificity, and demonstrates excellent repeatability and reproducibility. The results are in good agreement with RT-qPCR.
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