Method for simultaneously separating hematopoietic stem cells and platelet-rich plasma from umbilical cord blood

By combining two-step centrifugation with hydroxyethyl starch sedimentation and immunomagnetic bead sorting, the simultaneous separation of hematopoietic stem cells and platelet-rich plasma from umbilical cord blood is achieved, solving the problem that existing technologies cannot separate them simultaneously. This provides high-purity cells for clinical applications and reduces the risk of allogeneic immunization.

CN122445569APending Publication Date: 2026-07-24GUANGZHOU MUNICIPALITY TIANHE NUOYA BIO-ENG CO LTD
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Patent Information

Application Number
CN202610850017.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-06-12
Publication Date
2026-07-24

AI Technical Summary

Technical Problem

Current technology cannot efficiently separate clinical-grade hematopoietic stem cells and platelet-rich plasma from umbilical cord blood at the same time, resulting in resource waste and the risk of allogeneic immunity.

Method used

A two-step centrifugation method was adopted, combining hydroxyethyl starch sedimentation and immunomagnetic bead sorting. The plasma layer and the white membrane layer were separated by the first centrifugation, and platelet-rich plasma was prepared by the second centrifugation. Hematopoietic stem cells were obtained by washing and the separation was achieved simultaneously using a closed consumable system.

Benefits of technology

It simplifies the operating procedures, reduces the risk of contamination, avoids waste of resources, and provides high-purity hematopoietic stem cells and platelet-rich plasma, which are suitable for clinical combination therapy and reduce the risk of allogeneic immunity.

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Abstract

The invention belongs to the technical field of cell separation, and particularly relates to a method for simultaneously separating hematopoietic stem cells and platelet-rich plasma from one part of umbilical cord blood. Two-step centrifugation is adopted to replace traditional multiple centrifugal separation, operation is easy and convenient, time is shortened, the pollution risk is reduced, hematopoietic stem cells and platelet-rich plasma can be separated from one bag of umbilical cord blood at the same time, waste of umbilical cord blood resources is avoided, and the method is particularly suitable for precious samples or clinical combined treatment.
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