Metarhizium anisopliae gene MrFbx28 and application thereof

By knocking out the MrFbx28 gene of Metarhizium anisopliae, the sporulation rate, heat stress tolerance, and pathogenicity of the fungus are regulated, solving the problems of slow insecticidal rate and unstable control effect in existing technologies, and achieving a highly efficient biological control effect.

CN122445667APending Publication Date: 2026-07-24ANHUI AGRICULTURAL UNIVERSITY
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Patent Information

Application Number
CN202610836872.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-06-10
Publication Date
2026-07-24

AI Technical Summary

Technical Problem

The existing technology for treating Metarhizium anisopliae has a slow insecticidal rate, unstable control effect, and short shelf life. Furthermore, there are no reports on the biological functions of MrFbx28, which affects its large-scale application.

Method used

By reducing the expression level of the MrFbx28 gene mRNA or protein in Metarhizium anisopliae using gene knockout technology, the heat stress tolerance, sporulation rate, and pathogenicity of the fungus can be regulated, thereby constructing an engineered strain with increased sporulation rate, heat stress tolerance, and high virulence.

Benefits of technology

It increased the sporulation of Metarhizium anisopliae, enhanced its tolerance to heat stress and pathogenicity, and significantly improved its insecticidal efficacy.

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Abstract

The invention discloses a metarhizium anisopliae gene MrFbx28 and application thereof, particularly relates to the technical field of fungus molecular biology, and analyzes the biological function of MrFbx28 in metarhizium anisopliae. Based on yeast library screening, in combination with experimental techniques such as yeast two-hybridization, bimolecular fluorescence complementation, co-immunoprecipitation, co-localization and the like, the interaction relationship and the interaction site between the MrFbx28 and substrate proteins MrPck1 and MrHap5 are defined. Gene knockout and RNAi technologies are adopted, gene functions of substrate protein are further analyzed, immunoprecipitation and Western blot are combined, and it is clear that MrFbx28 regulates the ubiquitination level of substrate protein MrPck1 and MrHap5. Results clarify a regulation mechanism that metarhizium robertii MrFbx28 mediated substrate protein ubiquitination participates in heat stress response, and an application of the metarhizium robertii MrFbx28 mediated substrate protein ubiquitination in regulation of sporulation quantity and pathogenicity of fungi.
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