A cell climbing sheet processing reagent for a lupus nephritis / paranephritis antibody spectrum detection kit (cellular immunofluorescence method), a method thereof and application thereof

By using cell smear processing reagents and methods, simultaneous detection of multiple antibodies in Langefier nodule/paranodule was achieved, solving the problems of single detection mode and complex operation in existing technologies. This provides an efficient and accurate antibody detection solution, supporting precise subtyping and personalized treatment of ankylosing spondylitis (AN).

CN122448610APending Publication Date: 2026-07-24HEMAI (TIANJIN) MEDICAL TECHNOLOGY CO LTD
View PDF 0 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HEMAI (TIANJIN) MEDICAL TECHNOLOGY CO LTD
Filing Date
2026-06-29
Publication Date
2026-07-24

AI Technical Summary

Technical Problem

The existing Langfi nodal/paranodal antibody detection technology cannot achieve multi-antibody detection, which limits the accuracy of detection, is complicated to operate, and makes it difficult to meet the clinical needs of precise subtyping and personalized treatment of ankylosing spondylitis (AN).

Method used

Using cell slide processing reagents and methods, cell slides are treated with a specific ratio of cell fixative and a fixation antigen retrieval composition. Combined with mCherry fluorescent reporter tags, the simultaneous expression and detection of target antigens such as NF155, NF186, CNTN1, CASPR1, and MAG are achieved, and multi-target detection is performed using a biochip.

Benefits of technology

It enables simultaneous detection of five antibodies: NF155, NF186, CNTN1, CASPR1, and MAG, with a detection sensitivity of 100.00%, specificity of 95.00%, and overall accuracy of 96.88%, meeting the needs of large-scale clinical sample screening and rapid diagnosis.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN122448610A_ABST
    Figure CN122448610A_ABST
Patent Text Reader

Abstract

The application provides a cell climbing sheet processing reagent for a Ranvier node / paranode antibody spectrum detection kit (cellular immunofluorescence method), a method and application thereof, and relates to the technical field of in-vitro detection. The cell climbing sheet processing reagent comprises a cell fixing solution with a specific composition and an antigen repair composition after cell fixing, wherein the cell fixing solution is composed of paraformaldehyde, formaldehyde, acetic acid, raffinose and a buffer solution, and the antigen repair composition after cell fixing is composed of ammonium sulfate, hydroxylamine hydrochloride, Brij 78, TCEP, ProClin 300 and a buffer solution. Through the synergistic effect of the two, the natural conformation of the target antigen is maximally retained, and non-specific interference is reduced, so that precise and efficient detection of the Ranvier node / paranode antibody spectrum NF155, NF186, CNTN1, CASPR1 and MAG can be realized, the clinical diagnosis demand is met, and reliable technical support is provided for precise typing of autoimmune Ranvier node disease.
Need to check novelty before this filing date? Find Prior Art