A method for screening traditional Chinese medicine active ingredients based on anti-chemotherapy drug nephrotoxicity and drug interaction evaluation

Abstract

The application belongs to the technical field of drug screening, analytical chemistry and pharmacology, and specifically discloses a method for screening traditional Chinese medicine active ingredients based on anti-chemotherapy drug nephrotoxicity and evaluating drug interactions. By constructing an OCT2 high-expression stable cell strain, using styrene-maleic anhydride copolymer to form OCT2 nanoliposomes, and covalently fixing the nanoliposomes on ethylene sulfone-based silica gel, an OCT2 cell membrane chromatographic column with high stability and high activity is prepared. Based on the OCT2 cell membrane chromatographic column, a two-dimensional biochromatographic method for specifically screening OCT2 binding components from traditional Chinese medicine extracts is established. With OCT2 as a specific target, the application combines high-bionic cell membrane chromatography technology, two-dimensional chromatography-mass spectrometry technology, in-vitro binding kinetics analysis, competitive drug interaction evaluation, computer simulation and in-vivo pharmacodynamic verification, and forms a complete research system from discovery to drug interaction evaluation and then to in-vivo confirmation.

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