Ginseng and antler dipeptide directional enzymolysis preparation, separation and purification method

By using low-temperature gradient-assisted denaturation and stepwise directional enzymatic hydrolysis with complex proteases, the problem of synergistic processing of ginseng protein and deer antler collagen in the same enzymatic hydrolysis system was solved, achieving efficient preparation, separation and purification of dipeptides, improving the yield of dipeptides and maintaining the activity of ginseng protein.

CN122466048APending Publication Date: 2026-07-28李珂欣
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Patent Information

Application Number
CN202610775840.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-06-01
Publication Date
2026-07-28

AI Technical Summary

Technical Problem

Existing technologies cannot synergistically process ginseng protein and deer antler collagen in the same enzymatic hydrolysis system, resulting in low yield of the target dipeptide and difficulty in separation and purification. This is mainly because the dense triple helix structure of deer antler collagen requires high-temperature pre-denaturation treatment, which destroys the conformation of the active segment of ginseng protein.

Method used

Low-temperature gradient-assisted denaturation and stepwise directional enzymatic hydrolysis with complex proteases were employed. Mechanical energy was used to assist the reversible unfolding of deer antler collagen, maintaining the conformation of the active segment of ginseng protein unchanged. Stepwise directional enzymatic hydrolysis with alkaline protease, collagenase and aminopeptidase was used, combined with a three-step tandem separation of ultrafiltration, macroporous resin adsorption elution and reversed-phase chromatography.

Benefits of technology

This method enables the synergistic and targeted preparation and efficient separation and purification of ginseng protein and deer antler collagen in the same enzymatic hydrolysis system, improving the yield of the target dipeptide, avoiding the activity loss caused by high-temperature pre-denaturation, and solving the problem of difficult separation and purification.

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Abstract

The invention discloses a ginseng antler dipeptide directional enzymolysis preparation, separation and purification method, and belongs to the technical field of bioactive peptide preparation. Antler collagen is subjected to mechanical energy auxiliary treatment in an alkaline medium under the low-temperature condition lower than the thermal denaturation temperature of the antler collagen, so that a triple-helix structure is reversibly expanded to increase the accessible surface area of protease; meanwhile, the conformation of a ginseng protein active segment is reserved; the processed cornu cervi pantotrichum collagen and ginseng protein are prepared into a same substrate solution for synergetic enzymolysis; alkaline protease, collagenase and aminopeptidase are used for directional enzymolysis step by step, so that two characteristic dipeptides of prolyl-hydroxyproline and hydroxyprolyl-glycine are directionally enriched in a product; according to the method, low-temperature mechanical energy reversible expansion is used for replacing high-temperature pre-denaturation, the contradiction that treatment temperature windows of the two types of raw materials repel each other is relieved, the two types of raw materials are subjected to cooperative treatment in the same system, and the yield of the dipeptide higher than that of the dipeptide obtained through respective enzymolysis is obtained.
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