Auxiliary sample adding device for pcr (printed circuit board)

Abstract

The utility model discloses a pcr plate auxiliary sample adding device, which comprises a frame, division bars, a bar cover, a magnet 1, a freezing box and a 96-well plate, the top surface and the right side surface of the frame are opened, the top surface of the frame is divided into 12 bar-shaped grids by the division bars, and the positions of the bar-shaped grids are in one-to-one correspondence with the positions of 12 rows of holes on the pcr plate; strip covers are arranged on the strip-shaped grids, one ends of the strip covers are connected with the frame through hinges, and the other ends of the strip covers are movably connected with the frame through overturning magnets; a freezing box and a 96-well plate are sequentially inserted into the frame from bottom to top. The auxiliary sample adding device disclosed by the utility model overcomes the defects in the prior art that the requirements of low-temperature preparation of reagents, aerosol pollution of adjacent discharge holes and wrong / disordered adding cannot be met in the sample adding process of a pcr tube.

Description

Technical Field

[0001] The utility model belongs to the technical field of auxiliary tools for biological laboratories, and particularly relates to a PCR plate auxiliary sample adding device. Background Art

[0002] PCR detection technology has become the mainstream technology for various pathogen detection today. An important part of PCR detection operation is to add reagents or nucleic acid samples to eight-row tubes or 96-well plates. Adding reagents or nucleic acid samples properly is the basis for improving the quality of nucleic acid detection. Especially for large quantities of samples, it is necessary to avoid errors or confusion in sample addition to ensure the accuracy of the test results. The existing eight-row tubes or 96-well plates have the following shortcomings when adding samples: 1. If a counter is used, the operation is cumbersome and it is easy to make mistakes in a hurry; 2. There is no direct freezing device during the sample addition process, which cannot meet the requirements of low-temperature preparation of reagents; 3. When adding samples, if the sampled wells are not covered in time, it is easy to cause aerosol contamination of the wrong wells or adjacent wells; 4. The existing sample addition device cannot assist in fastening the eight-row tube cover. Therefore, we need an auxiliary sample addition device with a freezing device that can cover the unoperated wells and assist in covering the eight-row tube cover. Utility Model Content

[0003] Aiming at the above-mentioned deficiencies of the prior art, the utility model invents a PCR plate auxiliary sample adding device.

[0004] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0005] A PCR plate auxiliary sample loading device comprises a frame, a spacer bar, a strip cover, a magnet, a freezing box and a 96-well plate, wherein the top surface and the right side surface of the frame are open, and the top surface of the frame is divided into 12 strip grids by the spacer bar, and the positions of the strip grids correspond one to one with the positions of the 12 rows of holes on the PCR plate; a strip cover is arranged on the strip grid, one end of the strip cover is hinged to the frame, and the other end of the strip cover is movably connected to the frame through a flip magnet; a freezing box and a 96-well plate are inserted into the frame in sequence from bottom to top.

[0006] The auxiliary sample adding device of the utility model makes up for the shortcomings of the prior art in the process of adding samples to PCR tubes, such as failure to meet the requirements of low-temperature preparation of reagents, aerosol contamination of adjacent holes, and wrong addition / random addition.

[0007] Furthermore, it also includes a limiting connecting strip and a second magnet. The frame includes a main frame and an upper frame. The upper frame is movably arranged on the top of the main frame through the limiting connecting strip and the second magnet. The upper frame can be moved up and down so that a PCR plate filled with PCR tubes can be directly inserted into the frame from the side during use. After insertion, the upper frame is pressed down so that the bottom surface of the partition bar is in direct compression contact with the PCR plate to achieve a sealing effect, thereby avoiding the PCR tube being blocked by the lower edge of the partition bar due to insufficient space when inserting from the side.

[0008] Furthermore, sealing strips are provided on the upper and lower surfaces of the spacer strips, and the sealing strips are soft rubber strips or sponge strips, which isolate and seal the positions of the PCR plates or 96-well plates that they contact.

[0009] Furthermore, the freezing box is a freezing box with adjustable height. There are two heights of the freezing box. The shortest height can be used to directly insert the PCR plate for use. When multiple independent PCR tubes or eight-well tube strips need to be added separately, the freezing box can be adjusted to the highest height and the 96-well plate can be placed on top to insert a single PCR tube or eight-well tube strip for use.

[0010] Furthermore, it also includes an arch spring piece. The left and right sides of the inner side of the frame are embedded with arch spring pieces according to the positions of the freezing box and the 96-well plate. The function of the arch spring piece is to squeeze and fix the inserted freezing box, 96-well plate and PCR plate to prevent them from loosening and falling out.

[0011] The advantages of the utility model are: 1. The bottom of the device is equipped with a flat-bottomed aluminum freezer box with adjustable height, which can directly contact the bottom of the PCR plate to ensure the requirement of low-temperature storage of reagents. The freezer box can also be raised to directly place a single PCR tube or an eight-row tube on a 96-well plate for direct operation; 2. The device has 12 flip-up strip covers, which are kept in the closed state when no sample is added, and are covered in time after one column is added to prevent wrong addition, over-addition, and omission. More importantly, it can prevent cross-contamination of aerosols from adjacent samples. Keeping the cover down can also prevent contamination of reagents by aerosols in the environment; 3. All structures of the device are detachable, and the selected materials are acid-resistant, alkali-resistant, high temperature and high pressure-resistant, and can be disinfected in time after use to prevent cross-contamination in the laboratory. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 The utility model is a three-dimensional PCR plate auxiliary sample adding device Figure 1 ;

[0013] Figure 2 The utility model is a three-dimensional PCR plate auxiliary sample adding device (without strip cover) Figure 2 ;

[0014] Figure 3 It is a stereoscopic diagram of a frame in a PCR plate auxiliary sample adding device of the utility model. DETAILED DESCRIPTION

[0015] The utility model is described in detail below with reference to the accompanying drawings:

[0016] In the description of the present utility model, it should be noted that the directions or positional relationships indicated by "up", "down", "left" and "right" are based on the directions or positional relationships shown in the accompanying drawings, or are the directions or positional relationships in which the utility model product is usually placed when in use. They are only for the convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, and a specific direction structure and operation, and therefore cannot be understood as a limitation on the present utility model.

[0017] like Figure 1-3 As shown, a PCR plate auxiliary sample loading device includes a frame 1, a spacer bar 2, a strip cover 3, a magnet 1 4, a freezing box 5, a 96-well plate 6, a limit connecting strip 8 and a magnet 2 9. The top surface and the right side surface of the frame 1 are open, and the frame is in a rectangular shape. The top surface of the frame 1 is divided into 12 strip grids 7 by the spacer bar 2. The positions of the strip grids 7 correspond to the positions of the 12 rows of holes on the PCR plate. When the PCR plate is placed, the rows of holes on the plate are just in the strip grids 7. The thickness of the spacer bar 2 is 1.0 mm and the height is 2.0 mm, so that the height of the strip grid 7 will not be too high to affect the sample loading; sealing strips are arranged on the upper and lower sides of the spacer bar 2, which have a better sealing and separation effect on the PCR plate and a better isolation effect on aerosols. The strip grid 7 is provided with a strip cover 3, one end of which is hingedly connected to the frame 1, and the other end of which is movably connected to the frame 1 through a flip magnet 4. When adding samples, the corresponding strip cover 3 can be opened and covered after adding samples, which can prevent wrong addition and can also play a blocking role in the diffusion of aerosols. A freezing box 5 and a 96-well plate 6 are inserted into the frame 1 from bottom to top. The 96-well plate 6 is mainly used for adding samples to a single PCR tube or an eight-row tube.

[0018] In order to facilitate the insertion of the PCR plate so that the top of the PCR tube above is not blocked by the outermost partition bar 2, the frame 1 includes a main frame 11 and an upper frame 12. The upper frame 12 is arranged on the top of the main frame 11 to move up and down through a limiting connecting bar 8 and a magnet 9. The height of the upper frame 12 is 1.0cm~2.0cm, and it is mainly used to lift the height of the partition bar 2. After the PCR board is inserted, the partition bar 2 can be put down to separate the PCR board.

[0019] The freezing box 5 is a freezing box with adjustable height. The structure of the freezing box adopts an upper and lower cover structure. The upper cover and the lower cover are partially overlapped. The height of the freezing box can be increased by pulling apart the overlapping part. The connection between the upper and lower covers needs to be sealed.

[0020] In order to fix the freezing box 5, 96-well plate 6 and PCR plate inserted into the frame 1, an arch spring piece 10 is also provided in the frame 1. The arch spring piece 10 is provided on the left and right sides of the inner side of the frame 1. The specific position is embedded according to the position of the freezing box 5 and the 96-well plate 6. No matter how the height of the freezing box 5 changes, the inserted freezing box 5, 96-well plate 6 or PCR plate will be squeezed and fixed. Three horizontal and two rows of arch spring pieces 10 are provided on the left and right sides of the inner side of the frame 1; the arch spring piece 10 is provided in the groove on the left side of the inner side of the frame 1, and the two ends of the arch spring piece 10 are fixed, and the arched part thereof protrudes out of the groove.

[0021] The freezing box and 96-well plate in this embodiment are installed in a detachable manner, which is convenient for removal and disinfection and sterilization separately, and can avoid cross contamination caused by the transfer of items between different areas.

[0022] Although the specific embodiments of the present invention have been described and illustrated in detail above, it should be noted that various changes and modifications may be made to the above embodiments without departing from the spirit of the present invention and the scope of the appended claims.

Claims

1. A PCR plate auxiliary sample loading device, It is characterized in that The invention comprises a frame (1), a spacer bar (2), a strip cover (3), a magnet (4), a freezing box (5) and a 96-well plate (6); the top surface and the right side surface of the frame (1) are open; the top surface of the frame (1) is divided into 12 strip grids (7) by the spacer bar (2); the positions of the strip grids (7) correspond one to one with the positions of the 12 rows of holes on the PCR plate; a strip cover (3) is arranged on the strip grid (7); one end of the strip cover (3) is hingedly connected to the frame (1), and the other end of the strip cover (3) is movably connected to the frame (1) by a flip magnet (4); a freezing box (5) and a 96-well plate (6) are inserted into the frame (1) in sequence from bottom to top.

2. A PCR plate auxiliary sample loading device according to claim 1, It is characterized in that It also comprises a limiting connection strip (8) and a second magnet (9), and the frame (1) comprises a main frame (11) and an upper frame (12), and the upper frame (12) is movably arranged on the top of the main frame (11) via the limiting connection strip (8) and the second magnet (9) so as to move up and down.

3. A PCR plate auxiliary sample loading device according to claim 2, It is characterized in that Sealing strips are arranged on the upper and lower surfaces of the partition strip (2).

4. A PCR plate auxiliary sample loading device according to claim 3, It is characterized in that The freezing box (5) is a freezing box with adjustable height.

5. A PCR plate auxiliary sample loading device according to claim 4, It is characterized in that It also includes an arch spring piece (10), and the arch spring piece (10) is embedded on the left and right side surfaces of the inner side of the frame (1) according to the positions of the freezing box (5) and the 96-well plate (6).

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