Micro-fluidic chip for observing chlamydomonas
By designing the introduction channel and shut-off valve structure of the microfluidic chip, the problem of Chlamydomonas and drug residues in Chlamydomonas observation is solved, and uncontrolled inclusion is avoided during the observation process and the accuracy of the observation results is ensured.
Patent Information
- Application Number
- CN202420117903.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-01-17
- Publication Date
- 2025-01-10
- Estimated Expiration
- 2034-01-17
AI Technical Summary
During the observation of Chlamydomonas, the remaining Chlamydomonas and drugs in the material channel enter the container cavity uncontrollably, affecting the observation results.
A microfluidic chip for observing Chlamydomonas is designed, including a substrate, a cover plate, a first shut-off valve and a second shut-off valve. By operating these valves, the Chlamydomonas culture fluid and medicine fluid are introduced into the open guide channel, and discharged through the lead-off channel. For gases and excess liquids, use Chlamydomonas or drug fluid to fix Chlamydomonas on the fixing seat to avoid uncontrolled mixing.
Effectively close the introduction channel to prevent uncontrolled access to the receptacle cavity of Chlamydomonas and drugs, ensuring the accuracy of observation results.
Smart Images
Figure CN222331926U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of biological experimental equipment, in particular to a microfluidic chip for observing Chlamydomonas. Background Art
[0002] Microfluidic chips are a hot area in the development of micro-total analysis systems. Microfluidic chips are currently mainly used in life sciences and are the focus of development in the field of micro-total analysis systems. The goal is to integrate the functions of the entire laboratory, including sampling, dilution, reagent addition, reaction, separation, detection, etc., on a microfluidic chip that can be used multiple times. Due to different application functions, the style of microfluidic chips will change.
[0003] The existing microfluidic chip can be found in the patent with application number CN202222130431.2, which introduces the sample into the central accommodating chamber through various material channels, and then the sample in the accommodating chamber can be observed through the cover plate. In the experimental process of observing Chlamydomonas, it is necessary to first introduce Chlamydomonas and drugs into the accommodating chamber, and then observe the changes of Chlamydomonas under the action of drugs. However, during the observation process, the Chlamydomonas and drugs remaining in the material channel will still enter the accommodating chamber uncontrollably, affecting the observation results.
[0004] Therefore, how to prevent the Chlamydomonas residues and drugs in the material channel from entering the containing cavity uncontrollably during the observation of Chlamydomonas is a technical problem that needs to be solved urgently. Utility Model Content
[0005] The purpose of the utility model is to overcome the above-mentioned technical deficiencies, propose a microfluidic chip for observing Chlamydomonas, and solve the technical problem in the prior art of how to prevent the Chlamydomonas residues and drugs in the material channel from entering the containing cavity uncontrollably during the observation of Chlamydomonas.
[0006] In order to achieve the above technical objectives, the technical solution of the utility model includes a microfluidic chip for observing Chlamydomonas, which includes:
[0007] A substrate, the upper surface of which is provided with a receiving cavity, and a first inlet channel, a second inlet channel and an outlet channel communicating with the receiving cavity;
[0008] A cover plate, which is disposed on the upper surface of the substrate and covers the receiving cavity, the first inlet channel, the second inlet channel and the outlet channel;
[0009] A first stop valve, which controls the opening and closing of the first introduction channel;
[0010] A second stop valve, which controls the opening and closing of the second introduction channel; and
[0011] A plurality of fixing seats are arranged in a staggered manner in the receiving cavity.
[0012] Preferably, the cover plate is provided with a first inlet, a second inlet and a second outlet, and the first inlet, the second inlet and the second outlet are connected to the first inlet channel, the second inlet channel and the second outlet channel respectively.
[0013] Preferably, the first inlet channel has a first buffer chamber surrounding the first inlet port, the second inlet channel has a second buffer chamber surrounding the second inlet port, and the outlet channel has a third buffer chamber surrounding the outlet port.
[0014] Preferably, the fixing seat is provided with an arc groove connecting the two ends of the fixing seat in the vertical direction.
[0015] Preferably, the opening of the arc groove faces away from the outlet channel.
[0016] Preferably, the bottom end of the fixing seat is connected to the base plate, and an overflow gap is reserved between the top end of the fixing seat and the cover plate.
[0017] Preferably, the first stop valve includes a first spring sheet, one end of which is connected to the cover plate, and the other end of which is suspended on the first inlet channel, and the first spring sheet has a first state of contacting the substrate to close the first inlet channel, and a second state of detaching from the substrate and opening the first inlet channel, and the first spring sheet has an elastic force to switch to the second state, and the cover plate is provided with a first air guide hole, and the first spring sheet is pushed to overcome its own elastic force by introducing air flow into the first air guide hole.
[0018] Preferably, the second stop valve includes a second spring piece, one end of which is connected to the cover plate, and the other end of which is suspended on the second inlet channel, and the second spring piece has a third state of contacting the substrate to close the first inlet channel, and a fourth state of detaching from the substrate and opening the second inlet channel, and the second spring piece has an elastic force to switch to the fourth state, and the cover plate is provided with a second air guide hole, and the air flow is introduced into the second air guide hole to push the second spring piece to overcome its own elastic force.
[0019] Preferably, the first stop valve is installed at one end of the first introduction channel close to the accommodating chamber, and the second stop valve is installed at one end of the second introduction channel close to the accommodating chamber.
[0020] Preferably, the cover plate is a transparent cover plate.
[0021] Compared with the prior art, the beneficial effects of the utility model include: first, the first stop valve and the second stop valve are manipulated to open the first introduction channel and the second introduction channel. Then, the Chlamydomonas culture solution and the drug solution are introduced into the receiving chamber through the first introduction channel and the second introduction channel respectively, and the gas and excess liquid in the receiving chamber are discharged through the outlet channel, and then the first stop valve and the second stop valve can be manipulated to close one of the first introduction channel and the second introduction channel. Under the push of the Chlamydomonas culture solution or the drug liquid flow, the Chlamydomonas can be fixed on the fixed seat. Finally, the changes of the Chlamydomonas in the receiving chamber under the action of the drug can be observed through the cover plate. The microfluidic chip for observing Chlamydomonas provided by the utility model can be used to close one of the first introduction channel and the second introduction channel during the observation of Chlamydomonas, so as to avoid uncontrolled mixing of Chlamydomonas culture solution or drugs during the observation of Chlamydomonas and affect the final observation result. BRIEF DESCRIPTION OF THE DRAWINGS
[0022] Figure 1 This is a schematic diagram of the structure of a microfluidic chip for observing Chlamydomonas according to an embodiment of the utility model;
[0023] Figure 2 This is a partial schematic diagram of a fixing seat of an embodiment of the utility model;
[0024] Figure 3 This is a partial schematic diagram of the first stop valve of the utility model embodiment;
[0025] Figure 4 It is a partial schematic diagram of the second stop valve of the embodiment of the utility model;
[0026] Among them, the substrate 100, the accommodating cavity 110, the first inlet channel 120, the first buffer cavity 121, the second inlet channel 130, the second buffer cavity 131, the outlet channel 140, the third buffer cavity 141, the cover plate 200, the first inlet port 210, the second inlet port 220, the outlet port 230, the first air guide hole 240, the second air guide hole 250, the first stop valve 300, the first spring clip 310, the second stop valve 400, the second spring clip 410, the fixing seat 500, and the arc groove 510. DETAILED DESCRIPTION
[0027] In order to make the purpose, technical solution and advantages of the utility model more clear, the utility model is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the utility model and are not used to limit the utility model.
[0028] See also Figures 1 to 4The embodiment of the utility model provides a microfluidic chip for observing Chlamydomonas, which belongs to the field of biological experimental equipment. The microfluidic chip for observing Chlamydomonas is used to observe the changes of Chlamydomonas under the action of drugs. By using the above-mentioned microfluidic chip for observing Chlamydomonas, the introduction channel of Chlamydomonas and drug solution can be closed to avoid mixing of Chlamydomonas and drugs during the observation of Chlamydomonas and affect the final observation result.
[0029] In some preferred embodiments, a microfluidic chip for observing Chlamydomonas includes a substrate 100, a cover plate 200, a first stop valve 300 and a second stop valve 400, wherein a receiving cavity 110 is provided on the upper surface of the substrate 100, and a first inlet channel 120, a second inlet channel 130 and an outlet channel 140 are provided thereon to communicate with the receiving cavity 110. The cover plate 200 is covered on the upper surface of the substrate 100, and covers the receiving cavity 110, the first inlet channel 120, the second inlet channel 130 and the outlet channel 140. The first stop valve 300 controls the on-off of the first inlet channel 120. The second stop valve 400 controls the on-off of the second inlet channel 130.
[0030] In the above embodiment, the first stop valve 300 and the second stop valve 400 are first operated to open the first introduction channel 120 and the second introduction channel 130. Then, the Chlamydomonas culture solution and the drug solution are introduced into the receiving chamber 110 through the first introduction channel 120 and the second introduction channel 130 respectively, and the gas and excess liquid in the receiving chamber 110 are discharged through the outlet channel 140. Then, the first stop valve 300 and the second stop valve 400 can be operated to close one of the first introduction channel 120 and the second introduction channel 130. Under the impetus of the Chlamydomonas culture solution or drug flow, the Chlamydomonas can be fixed on the fixing seat 500. Finally, the changes of the Chlamydomonas in the receiving chamber 110 under the action of the drug can be observed through the cover plate 200. Using the microfluidic chip for observing Chlamydomonas provided by the utility model, one of the first introduction channel 120 and the second introduction channel 130 can be closed during the observation of Chlamydomonas, so as to avoid uncontrolled mixing of Chlamydomonas culture solution or drugs during the observation of Chlamydomonas and affect the final observation result.
[0031] In some preferred embodiments, the cover plate 200 is provided with a first inlet 210, a second inlet 220 and an outlet 230, and the first inlet 210, the second inlet 220 and the outlet 230 are respectively connected to the first inlet channel 120, the second inlet channel 130 and the outlet channel 140. In the above embodiment, the Chlamydomonas culture solution enters the first inlet channel 120 through the first inlet 210, the drug solution enters the second inlet channel 130 through the second inlet 220, and at the same time, the gas and liquid in the outlet channel 140 can be discharged through the outlet 230.
[0032] Based on the above embodiments, in some preferred embodiments, the first inlet channel 120 has a first buffer cavity 121 surrounding the first inlet port 210 , the second inlet channel 130 has a second buffer cavity 131 surrounding the second inlet port 220 , and the outlet channel 140 has a third buffer cavity 141 surrounding the outlet port 230 .
[0033] In the above embodiment, since the first buffer chamber 121, the second buffer chamber 131 and the third buffer chamber 141 surround the first inlet 210, the second inlet 220 and the outlet 230 respectively, the first buffer chamber 121, the second buffer chamber 131 and the third buffer chamber 141 can buffer the materials at their respective joints.
[0034] In some preferred embodiments, the microfluidic chip for observing Chlamydomonas further comprises a plurality of fixing seats 500 for capturing Chlamydomonas, and the plurality of fixing seats 500 are staggeredly arranged in the receiving chamber 110. The fixing seats 500 can block and fix Chlamydomonas, so that Chlamydomonas is in a static state, so as to facilitate the observation of Chlamydomonas.
[0035] On the basis of the above embodiments, in some preferred embodiments, the fixing seat 500 is provided with an arc groove 510 connecting the two ends of the fixing seat 500 in the vertical direction. Thus, the arc groove 510 can be used to restrict the movement of Chlamydomonas.
[0036] In addition, in some preferred embodiments, the opening of the arc groove 510 is away from the outlet channel 140, so that the opening of the arc groove 510 can receive the fluid in the receiving chamber 110, so that the Chlamydomonas can enter the arc groove 510 driven by the fluid.
[0037] In some preferred embodiments, the top of the fixing seat 500 is connected to the cover plate 200, and an overflow gap is reserved between the bottom end of the fixing seat 500 and the substrate 100. Since an overflow gap is reserved between the bottom end of the fixing seat 500 and the substrate 100, the fluid in the receiving chamber 110 will pass through the overflow gap, so that the fluid can drive the Chlamydomonas into the arc groove 510. However, it should be emphasized that the height of the overflow gap needs to be small enough to prevent the Chlamydomonas from passing through the overflow gap. It can be understood that the fixing seat 500 can be connected not only to the cover plate 200, but also to the substrate 100, and the corresponding fixing seat 500 needs to retain an overflow gap with the cover plate 200.
[0038] As long as the implementation method of the first stop valve 300 can close the first inlet channel 120, it is feasible. In some preferred embodiments, the first stop valve 300 includes a first spring piece 310, one end of the first spring piece 310 is connected to the cover plate 200, and the other end is suspended on the first inlet channel 120, and the first spring piece 310 has a first state of contacting the substrate 100 to close the first inlet channel 120, and a second state of detaching from the substrate 100 and opening the first inlet channel, and the first spring piece 310 has an elastic force to switch to the second state, and the cover plate 200 is provided with a first air guide hole 240, and the air flow is introduced into the first air guide hole 240 to push the first spring piece 310 to overcome its own elastic force.
[0039] As long as the implementation method of the second stop valve 400 can close the second inlet channel 130, it is feasible. In some preferred embodiments, the second stop valve 400 includes a second spring piece 410, one end of the second spring piece 410 is connected to the cover plate 200, and the other end is suspended on the second inlet channel 130, and the second spring piece 410 has a third state of contacting the substrate 100 to close the first inlet channel 120, and a fourth state of detaching from the substrate 100 and opening the second inlet channel, and the second spring piece 410 has an elastic force to switch to the fourth state, and the cover plate 200 is provided with a second air guide hole 250, and the air flow is introduced into the second air guide hole 250 to push the second spring piece 410 to overcome its own elastic force.
[0040] In some preferred embodiments, the first stop valve 300 is installed at one end of the first introduction channel 120 close to the receiving chamber 110, and the second stop valve 400 is installed at one end of the second introduction channel 130 close to the receiving chamber 110. In this way, the Chlamydomonas remaining in the first introduction channel 120 can be prevented from entering the receiving chamber 110, and the Chlamydomonas remaining in the second introduction channel 130 can also be prevented from entering the receiving chamber 110.
[0041] First, the first stop valve 300 and the second stop valve 400 are operated to open the first introduction channel 120 and the second introduction channel 130. Then, the Chlamydomonas culture solution and the drug solution are introduced into the receiving chamber 110 through the first introduction channel 120 and the second introduction channel 130 respectively, and the gas and excess liquid in the receiving chamber 110 are discharged through the outlet channel 140. Then, the first stop valve 300 and the second stop valve 400 can be operated to close one of the first introduction channel 120 and the second introduction channel 130. Under the impetus of the Chlamydomonas culture solution or drug flow, the Chlamydomonas can be fixed on the fixing seat 500. Finally, the changes of the Chlamydomonas in the receiving chamber 110 under the action of the drug can be observed through the cover plate 200. Using the microfluidic chip for observing Chlamydomonas provided by the utility model, one of the first introduction channel 120 and the second introduction channel 130 can be closed during the observation of Chlamydomonas, so as to avoid uncontrolled mixing of Chlamydomonas culture solution or drugs during the observation of Chlamydomonas and affect the final observation result.
[0042] The specific implementation methods of the utility model described above do not constitute a limitation on the protection scope of the utility model. Any other corresponding changes and modifications made according to the technical concept of the utility model should be included in the protection scope of the claims of the utility model.
Claims
1. A microfluidic chip for observing Chlamydomonas, characterized in that: include: A substrate, the upper surface of which is provided with a receiving cavity, and a first inlet channel, a second inlet channel and an outlet channel communicating with the receiving cavity; A cover plate, which is disposed on the upper surface of the substrate and covers the receiving cavity, the first inlet channel, the second inlet channel and the outlet channel; A first stop valve, which controls the opening and closing of the first introduction channel; A second stop valve, which controls the on and off of the second introduction channel; as well as A plurality of fixing seats are arranged in a staggered manner in the receiving cavity.
2. The microfluidic chip for observing Chlamydomonas according to claim 1, characterized in that: The cover plate is provided with a first inlet, a second inlet and a second outlet, and the first inlet, the second inlet and the second outlet are connected to the first inlet channel, the second inlet channel and the second outlet channel respectively.
3. The microfluidic chip for observing Chlamydomonas according to claim 2, characterized in that: The first inlet channel has a first buffer chamber surrounding the first inlet port, the second inlet channel has a second buffer chamber surrounding the second inlet port, and the outlet channel has a third buffer chamber surrounding the outlet port.
4. The microfluidic chip for observing Chlamydomonas according to claim 1, characterized in that: The fixing seat is provided with an arc groove connecting the two ends of the fixing seat along the vertical direction.
5. The microfluidic chip for observing Chlamydomonas according to claim 4, characterized in that: The opening of the circular arc groove is away from the outlet channel.
6. The microfluidic chip for observing Chlamydomonas according to claim 1, characterized in that: The bottom end of the fixing seat is connected to the base plate, and an overflow gap is reserved between the top end of the fixing seat and the cover plate.
7. The microfluidic chip for observing Chlamydomonas according to claim 1, characterized in that: The first stop valve includes a first spring sheet, one end of which is connected to the cover plate, and the other end of which is suspended on the first introduction channel. The first spring sheet has a first state of contacting the substrate to close the first introduction channel, and a second state of detaching from the substrate and opening the first introduction channel. The first spring sheet has an elastic force to switch to the second state. The cover plate is provided with a first air guide hole, and the air flow is introduced into the first air guide hole to push the first spring sheet to overcome its own elastic force.
8. The microfluidic chip for observing Chlamydomonas according to claim 1, characterized in that: The second stop valve includes a second spring piece, one end of which is connected to the cover plate, and the other end of which is suspended on the second inlet channel. The second spring piece has a third state of contacting the substrate to close the first inlet channel, and a fourth state of detaching from the substrate and opening the second inlet channel. The second spring piece has an elastic force for switching to the fourth state. The cover plate is provided with a second air guide hole, and the air flow is introduced into the second air guide hole to push the second spring piece to overcome its own elastic force.
9. The microfluidic chip for observing Chlamydomonas according to claim 1, characterized in that: The first stop valve is installed at one end of the first introduction channel close to the receiving chamber, and the second stop valve is installed at one end of the second introduction channel close to the receiving chamber.
10. The microfluidic chip for observing Chlamydomonas according to claim 1, characterized in that: The cover plate is a transparent cover plate.
Citation Information
Patent Citations
Microfluidic chip
CN218459549U