Non-sporogenic aspergillus solid state fermentation inoculation stirrer

Abstract

The utility model relates to the technical field of fermentation inoculation tools, in particular to a non-sporogenic aspergillus solid-state fermentation inoculation stirrer which comprises a shell, the shell is of a cylindrical structure, a liquid absorption ball is installed at one end of the shell, a liquid storage cavity is installed at the other end of the shell, a stirring rod is arranged in the shell, and a liquid absorption opening is formed in the outer side of the liquid storage cavity. A limiting plate is mounted on the outer wall of the stirring rod, a limiting plate is mounted at the position, close to the liquid suction ball, of the inner wall of the shell, a groove is formed in the outer end of the limiting plate, a clamping spring is arranged in the groove, a movable clamping block is mounted at the outer end of the clamping spring, a limiting opening is formed in the position, close to the liquid storage cavity, of the inner wall of the shell, and the outer end of the stirring rod slides after being stressed; and one end extends out of the liquid suction port to drive the movable clamping block to be clamped into the limiting port. According to the solid-state fermentation inoculation stirrer for the aspergillus sporogenes, the technical problems in the traditional inoculation and stirring process are effectively solved, the inoculation efficiency and the stirring uniformity are remarkably improved, and the pollution risk is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of fermentation inoculation tools, in particular to a non-spore-producing Aspergillus solid-state fermentation inoculation stirrer. Background Art

[0002] In the field of bioengineering and fermentation technology, Aspergillus niger is widely used because it can produce a variety of industrial enzymes. However, traditional Aspergillus niger produces melanin during the fermentation process, which increases the difficulty and cost of subsequent enzyme purification or product separation. In order to solve this problem, researchers interrupted the melanin gene of Aspergillus niger through genetic modification or mutagenesis technology and cultivated Aspergillus niger that does not produce spores and white mycelium.

[0003] During the solid-state fermentation preparation process of this new type of Aspergillus niger, it is necessary to first prepare the liquid fermentation seed liquid of mycelium, and then inoculate it into the solid-state fermentation medium. However, there are many challenges in the inoculation and stirring process. Due to the large diameter of the mycelium, the aperture of the common pipette tip on the market is too small to be directly absorbed. At the same time, it needs to be stirred evenly as soon as possible after inoculation to prevent contamination, but traditional stirring methods, such as using glass rods or stainless steel rods, not only increase the risk of contamination, but also waste inoculation time. Utility Model Content

[0004] The purpose of the utility model is to provide a non-spore-forming Aspergillus solid-state fermentation inoculation stirrer to solve the many challenges in the inoculation and stirring process proposed in the above-mentioned background technology. Due to the large diameter of the mycelium, the aperture of the common pipette tip on the market is too small to directly absorb. At the same time, it is necessary to stir evenly as soon as possible after inoculation to prevent contamination, but traditional stirring methods, such as using glass rods or stainless steel rods, not only increase the risk of contamination, but also waste inoculation time.

[0005] To achieve the above-mentioned purpose, the utility model provides a non-spore-producing Aspergillus solid-state fermentation inoculation agitator, comprising an outer shell, which has a cylindrical structure, a liquid absorption ball installed at one end, and a liquid storage cavity installed at the other end, a stirring rod is arranged inside the outer shell, a liquid absorption port is arranged on the outer side of the liquid storage cavity, an ear plate is arranged on the outer wall of the stirring rod, a limiting plate is arranged on the inner wall of the outer shell near the liquid absorption ball, a groove is arranged on the outer end of the ear plate, a clamping spring is arranged inside the groove, a movable clamping block is arranged on the outer end of the clamping spring, and a limiting port is arranged on the inner wall of the outer shell near the liquid storage cavity. The outer end of the stirring rod slides after being subjected to force, and one end extends out of the liquid absorption port, thereby driving the movable clamping block to be clamped into the limiting port.

[0006] Preferably, the outer end of the movable block is provided with a curved surface.

[0007] Preferably, a return spring is provided between the outer end of the liquid storage chamber and the ear plate.

[0008] Preferably, a guide cylinder is provided at one end of the liquid storage chamber close to the stirring rod, and the stirring rod and the guide cylinder are slidably arranged.

[0009] Preferably, a reset spring is installed on the outer wall of one side of the shell, one end of the reset spring is welded and fixed to the shell, and the other end is installed with a protrusion, which is located at the limit opening. After the reset spring is subjected to force, it drives the protrusion to move toward the inside of the limit opening, and then presses the movable block, so that the movable block is separated from the limit opening.

[0010] Preferably, the length of the stirring rod extending out of the liquid suction port is 50 mm.

[0011] Preferably, the diameter of the liquid suction port is 3.2 mm, and the diameter of the stirring rod is matched with the liquid suction port.

[0012] Preferably, the shell is made of stainless steel, and a cover is threadedly connected to one end. The liquid storage chamber is divided into two parts connected by threads, and the outer wall is provided with scale lines.

[0013] Compared with the prior art, the utility model has the following beneficial effects:

[0014] The non-spore-forming Aspergillus solid-state fermentation inoculation agitator effectively solves the technical difficulties in the traditional inoculation and stirring process, significantly improves the inoculation efficiency and stirring uniformity, and reduces the risk of contamination. Specifically, the agitator achieves the dual functions of quantitative inoculation and rapid stirring through a unique design, which is particularly suitable for the characteristics of non-spore-forming Aspergillus niger mycelium.

[0015] First, the aspiration port of the stirrer is reasonably designed with a moderate aperture, which can directly absorb the mycelium, avoiding the problem that the traditional pipette tip has a small aperture and cannot absorb. At the same time, the design of the stirring rod also fully considers the characteristics of the mycelium, and can quickly stir the mycelium and the culture medium evenly after inoculation, thereby improving the fermentation efficiency.

[0016] Secondly, the operation of the stirrer is simple and quick, which can greatly reduce the inoculation time and stirring time, thereby reducing the time of opening the solid-state fermentation medium and reducing the risk of contamination. In addition, the structure design of the stirrer is reasonable and easy to use, and it can be widely used in solid-state fermentation inoculation work of various scales.

[0017] Finally, the agitator is made of stainless steel material, which has good corrosion resistance and durability, can be used for a long time, and reduces the cost of equipment replacement. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a structural schematic diagram of the stirring rod retracted in the utility model;

[0019] Figure 2 This is a schematic diagram of the structure in which the stirring rod in the utility model is extended;

[0020] Figure 3 This is a schematic diagram of the structure of the limiting plate in the utility model;

[0021] Figure 4 It is a structural schematic diagram of the housing in the utility model;

[0022] Figure 5 It is a structural schematic diagram of the liquid storage chamber in the utility model.

[0023] The meaning of each number in the figure is:

[0024] 1. Shell; 11. Limit plate; 12. Limit port; 13. Reset spring; 14. Cover; 2. Stirring rod; 21. Ear plate; 22. Movable clamping block; 221. Arc surface; 23. Clamping spring; 3. Liquid suction ball; 4. Reset spring; 5. Liquid storage chamber; 51. Liquid suction port; 52. Guide cylinder. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0026] The utility model provides a non-spore-producing Aspergillus solid-state fermentation inoculation stirrer. Figure 1-Figure 5 As shown, it includes an outer shell 1, which has a cylindrical structure, with a liquid absorbing ball 3 installed at one end and a liquid storage chamber 5 installed at the other end. A stirring rod 2 is arranged inside the outer shell 1, and a liquid suction port 51 is arranged on the outer side of the liquid storage chamber 5 for sucking liquid. An ear plate 21 is installed on the outer wall of the stirring rod 2, and a limiting plate 11 is installed on the inner wall of the outer shell 1 near the liquid absorbing ball 3. A groove is arranged at the outer end of the ear plate 21, and a clamping spring 23 is arranged inside the groove. A movable clamping block 22 is installed on the outer end of the clamping spring 23, and a limiting port 12 is arranged on the inner wall of the outer shell 1 near the liquid storage chamber 5. The outer end of the stirring rod 2 slides after being subjected to force, and one end extends out of the liquid suction port 51, thereby driving the movable clamping block 22 to be clamped into the limiting port 12, so that the stirring rod 2 remains in an extended state for convenient stirring.

[0027] In this embodiment, the outer end of the movable block 22 is provided with a curved surface 221 to facilitate the movable block 22 to be inserted into the limiting opening 12 .

[0028] Specifically, a return spring 4 is provided between the outer end of the liquid storage chamber 5 and the ear plate 21 to facilitate the return and retraction of the stirring rod 2 .

[0029] Furthermore, a guide cylinder 52 is provided at one end of the liquid storage chamber 5 close to the stirring rod 2 , and the stirring rod 2 and the guide cylinder 52 are slidably arranged.

[0030] Furthermore, a reset spring 13 is installed on the outer wall of one side of the shell 1, one end of the reset spring 13 is welded and fixed to the shell 1, and the other end is installed with a protrusion, which is located at the limit opening 12. After the reset spring 13 is subjected to force, it drives the protrusion to move toward the inside of the limit opening 12, and then presses the movable block 22, so that the movable block 22 is separated from the limit opening 12, and the stirring rod 2 can be reset and retracted by pressing the reset spring 13.

[0031] Furthermore, the length of the stirring rod 2 extending out of the liquid suction port 51 is 50 mm.

[0032] Furthermore, the diameter of the liquid suction port 51 is 3.2 mm, and the diameter of the stirring rod 2 is matched with the liquid suction port 51 , so that the material on the outer wall can be scraped off through the liquid suction port 51 when the stirring rod 2 is retracted.

[0033] Furthermore, the housing 1 is made of stainless steel, and a cover 14 is threadedly connected to one end. The liquid storage chamber 5 is divided into two parts connected by threads, and the outer wall is provided with scale lines for easy observation of the scale of the liquid storage.

[0034] When the non-spore-forming Aspergillus solid-state fermentation inoculation stirrer of the utility model is used, first, when inoculation is required, the stirring rod 2 is pushed so that it slides inside the housing 1 after being subjected to force, and one end extends out of the liquid suction port 51. During the extension process, the movable clamping block 22 is clamped into the limit opening 12 under the action of the clamping spring 23, thereby keeping the stirring rod 2 in the extended state.

[0035] At this time, the stirring rod 2 can be extended into the solid fermentation medium for stirring, so that the mycelium and the medium are quickly mixed evenly. Since the diameter of the stirring rod 2 is compatible with the liquid suction port 51, when the stirring rod 2 is retracted, the material on its outer wall can be scraped off to avoid pollution and waste.

[0036] After the inoculation and stirring are completed, the reset spring 13 is pressed to drive the protrusion to move to the inside of the limit opening 12 after being subjected to force, and then the movable block 22 is pressed to separate the movable block 22 from the limit opening 12. At this time, the stirring rod 2 automatically resets and retracts under the action of the reset spring 4, and returns to the initial state, ready for the next inoculation and stirring. Then, the liquid fermentation seed liquid of the mycelium to be inoculated is sucked into the liquid storage chamber 5 by pressing the liquid absorption ball 3. Since the diameter of the liquid absorption port 51 is moderate, the mycelium with a larger diameter can be directly absorbed, avoiding the problem that the traditional pipette tip has a small aperture and cannot be absorbed.

[0037] During the whole process, the housing 1 is made of stainless steel, has good corrosion resistance and durability, and can be used for a long time. Meanwhile, the outer wall of the liquid storage chamber 5 is provided with scale lines, which is convenient for observing the scale of the liquid storage and realizing quantitative inoculation.

[0038] The above shows and describes the basic principle, main features and advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments. The above embodiments and descriptions are only preferred examples of the utility model and are not used to limit the utility model. Without departing from the spirit and scope of the utility model, the utility model may have various changes and improvements, which fall within the scope of the utility model to be protected. The scope of protection of the utility model is defined by the attached claims and their equivalents.

Claims

1. A non-spore-forming Aspergillus solid-state fermentation inoculation stirrer, comprising a housing (1), characterized in that: The shell (1) is a cylindrical structure, with a liquid absorbing ball (3) installed at one end and a liquid storage chamber (5) installed at the other end. A stirring rod (2) is arranged inside the shell (1), and a liquid suction port (51) is arranged outside the liquid storage chamber (5). An ear plate (21) is arranged on the outer wall of the stirring rod (2). A limiting plate (11) is arranged on the inner wall of the shell (1) near the liquid absorbing ball (3). A groove is arranged at the outer end of the ear plate (21), and a clamping spring (23) is arranged inside the groove. A movable clamping block (22) is arranged on the outer end of the clamping spring (23). A limiting port (12) is arranged on the inner wall of the shell (1) near the liquid storage chamber (5). The outer end of the stirring rod (2) slides after being subjected to force, and one end extends out of the liquid suction port (51), thereby driving the movable clamping block (22) to be clamped into the limiting port (12).

2. The non-spore-forming Aspergillus solid-state fermentation inoculation stirrer according to claim 1, characterized in that: The outer end of the movable clamping block (22) is provided with a curved surface (221).

3. The non-spore-forming Aspergillus solid-state fermentation inoculation stirrer according to claim 1, characterized in that: A return spring (4) is arranged between the outer end of the liquid storage chamber (5) and the ear plate (21).

4. The non-spore-forming Aspergillus solid-state fermentation inoculation stirrer according to claim 1, characterized in that: A guide cylinder (52) is provided at one end of the liquid storage chamber (5) close to the stirring rod (2), and the stirring rod (2) and the guide cylinder (52) are slidably arranged.

5. The non-spore-forming Aspergillus solid-state fermentation inoculation stirrer according to claim 1, characterized in that: A reset spring (13) is installed on one side outer wall of the housing (1); one end of the reset spring (13) is welded and fixed to the housing (1); the other end of the reset spring (13) is installed with a protrusion, which is located at the limiting opening (12); when the reset spring (13) is subjected to force, it drives the protrusion to move toward the inside of the limiting opening (12), thereby pressing the movable clamping block (22), so that the movable clamping block (22) is separated from the limiting opening (12).

6. The non-spore-forming Aspergillus solid-state fermentation inoculation stirrer according to claim 1, characterized in that: The length of the stirring rod (2) extending out of the liquid suction port (51) is 50 mm.

7. The non-spore-forming Aspergillus solid-state fermentation inoculation stirrer according to claim 6, characterized in that: The diameter of the liquid suction port (51) is 3.2 mm, and the diameter of the stirring rod (2) is compatible with the liquid suction port (51).

8. The non-spore-forming Aspergillus solid-state fermentation inoculation stirrer according to claim 1, characterized in that: The housing (1) is made of stainless steel and has a cover (14) threadedly connected to one end. The liquid storage chamber (5) is divided into two parts that are threadedly connected and the outer wall is provided with scale lines.

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