Dual-mode microscopic hyperspectral imaging system for floating algae detection

Abstract

The utility model provides a dual-mode microscopic hyperspectral imaging system for detecting floating algae, which is used for detecting and imaging algae samples and comprises a CMOS (complementary metal oxide semiconductor) camera, an eyepiece, an optical filter rotating wheel, a band-pass optical filter, a beam splitter, a microscope objective, a transparent bearing sheet, a collimation light source, a beam expander, a long-focus lens and a laser. The CMOS camera, the eyepiece, the optical filter rotating wheel, the beam splitter, the microscope objective, the transparent bearing sheet and the collimation light source are sequentially arranged from top to bottom; the device has two imaging modes, a fluorescence image of an algae sample can be obtained when the laser works, and a transmission spectrum image of the algae sample can be obtained when the collimation light source works. When the device is used for processing complex biological samples, the defect that an existing device has a single fluorescence imaging mode in the aspect of a measurement mode is overcome, the detection requirements of diversified samples are met, and efficient detection of different types of samples is realized.

Description

Technical Field

[0001] The utility model belongs to the technical field of phytoplankton detection, and specifically relates to a dual-mode microscopic hyperspectral imaging system for phytoplankton detection. Background Art

[0002] The rapid development of industry and urbanization has damaged my country's water ecological environment and caused imbalance in river basin ecosystems. Under the influence of climate change and human activities, the problem of algal blooms in lakes, especially eutrophic lakes, is difficult to solve in the short term and will continue to be an important lake ecological problem. Harmful algae such as red tides and algal blooms will cause serious economic losses. Therefore, it is crucial to establish methods for identifying and monitoring microalgae. In the Chinese utility model patent authorization announcement number "CN214122000U", the patent name is "A phytoplankton detection device". The device has a complex light source, a sampling component and a fluorescence detector. The fluorescence intensity distribution data of the algae group is obtained through the superposition effect of characteristic fluorescence intensity, and finally the detection of phytoplankton in the regional range is realized. However, the utility model patent is limited to a single fluorescence imaging mode in the detection mode, which is incapable of processing complex biological samples and cannot meet the detection needs of diversified samples. Therefore, a new solution is urgently needed in the prior art to solve the above problems. Utility Model Content

[0003] The problem to be solved by the utility model is: how to provide a device integrating transmission imaging and fluorescence imaging modes to realize efficient detection of algae samples.

[0004] A dual-mode microscopic hyperspectral imaging system for phytoplankton detection, used for detection and imaging of algae samples, comprising a CMOS camera, an eyepiece, a filter wheel, a bandpass filter, a beam splitter, a microscope objective, a transparent carrier, a collimated light source, a beam expander, a telephoto lens and a laser;

[0005] The CMOS camera, the eyepiece, the filter wheel, the beam splitter, the microscope objective lens, the transparent carrier sheet and the collimated light source are arranged in sequence from top to bottom;

[0006] Six bandpass filters are arranged on the filter wheel, the six bandpass filters are in the same plane, and the filter wheel is used to drive the bandpass filters to be located between the eyepiece and the beam splitter;

[0007] The algae sample is disposed on the transparent carrier sheet;

[0008] The laser is located on the side of the beam splitter, and a beam expander and a telephoto lens are arranged in sequence between the laser and the beam splitter; the laser is used to emit light, which passes through the beam expander and the telephoto lens, and then part of the light is reflected by the beam splitter and passes through the microscope objective lens to irradiate the algae sample to generate a fluorescent signal, and the fluorescent signal enters the microscope objective lens and is imaged on the CMOS camera;

[0009] The collimated light source is used to emit light that passes through the algae sample to generate a transmission signal that enters the microscope objective lens and is imaged by a CMOS camera.

[0010] The transparent carrier sheet is a microfluidic chip.

[0011] The six bandpass filters have the same diameter.

[0012] The six bandpass filters are bandpass filters with a full width at half maximum (FWHM) of 10 nm, and the wavelength bands thereof are 630 nm, 650 nm, 610 nm, 490 nm, 590 nm and 530 nm respectively.

[0013] The wavelength of the laser is 532 nm.

[0014] The beneficial effects of the present application are as follows: the present invention has two imaging modes, when the laser is working, a fluorescent image of the algae sample can be obtained, and when the collimated light source is working, a transmission spectrum image of the algae sample can be obtained. Through one device, two types of information required for the detection and identification of algae samples can be conveniently obtained, wherein the obtained spectrum image is for the purpose of identifying and classifying different types of algae, and the fluorescent image produced by laser excitation of algae is for distinguishing algae from impurity particles in the field of view. When processing complex biological samples, the present invention makes up for the deficiency of the existing device in the single fluorescence imaging mode in the measurement mode, meets the detection needs of diversified samples, and realizes efficient detection of different types of samples. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 The utility model is a schematic diagram of the structure of a dual-mode microscopic hyperspectral imaging system for phytoplankton detection. DETAILED DESCRIPTION

[0016] The utility model proposes a dual-mode microscopic hyperspectral imaging system for phytoplankton detection, which is used for detection and imaging of algae samples, such as Figure 1 As shown, it includes a CMOS camera 1, an eyepiece 2, a filter wheel 3, a bandpass filter 4, a beam splitter 5, a microscope objective 6, a transparent carrier 7, a laser 8, a beam expander 9, a telephoto lens 10 and a collimated light source 11;

[0017] The CMOS camera 1, the eyepiece 2, the filter wheel 3, the beam splitter 5, the microscope objective 6, the transparent carrier sheet 7 and the collimated light source 11 are arranged in sequence from top to bottom;

[0018] Six bandpass filters 4 are arranged on the filter wheel 3, and the six bandpass filters 4 are in the same plane. The filter wheel 3 is used to drive the bandpass filters 4 to be located between the eyepiece 2 and the beam splitter 5;

[0019] The algae sample is disposed on the transparent carrier sheet 7;

[0020] The laser 8 is located on the side of the beam splitter 5, and a beam expander 9 and a telephoto lens 10 are sequentially arranged between the laser 8 and the beam splitter 5; the laser 8 is used to emit light, which passes through the beam expander 9 and the telephoto lens 10, and then is reflected by the beam splitter 5, and part of the light passes through the microscope objective lens 6 to irradiate the algae sample to generate a fluorescent signal, and the fluorescent signal enters the microscope objective lens 6 and is imaged on the CMOS camera 1;

[0021] The collimated light source 11 is used to emit light that passes through the algae sample to generate a transmission signal, enters the microscope objective lens 6 , and is imaged on the CMOS camera 1 .

[0022] The transparent carrier sheet 7 is a microfluidic chip.

[0023] The six band-pass filters 4 have the same diameter.

[0024] The six bandpass filters 4 are bandpass filters with a full width at half maximum (FWHM) of 10 nm, and the wavelength bands thereof are 630 nm, 650 nm, 610 nm, 490 nm, 590 nm and 530 nm respectively.

[0025] The wavelength of the laser 8 is 532 nm.

[0026] The beam splitter 5 is preferably a product of the Lubang brand, model CM-3R. The beam splitter 5 is equipped with a beam splitter, which plays a semi-reflective and semi-transmissive role for the light beams emitted by the laser 8 and the collimated light source 11.

[0027] The microfluidic chip is produced by FluidicLab and has a model number of PDMS-FF-30.

[0028] The utility model has two imaging modes. In the fluorescence imaging mode, the laser 8 with a wavelength of 532nm is preferably used to obtain a better fluorescence effect. The transparent carrier sheet 7 is a microfluidic chip. The algae sample is placed on the microfluidic chip. When the algae sample fluorescence signal imaging needs to be obtained, the collimated light source 11 does not work. Figure 1As shown, the laser 8 is located on the side of the beam splitter 5. The light emitted by the laser 8 is reflected by the beam splitter 5 through the beam expander 9 and the telephoto lens 10, and then part of the light is irradiated on the algae sample in the microfluidic chip collection pool by the microscope objective lens 6 in a falling manner. The algae sample generates a fluorescent signal, which is collected by the microscope objective lens 6 and then emitted. Then, it passes through the beam splitter 5, and the transmitted part passes through the bandpass filter 4 to filter out the influence of the laser, and then enters the eyepiece 2. Finally, the light converges to the CMOS camera 1. The fluorescent image information of the algae can be obtained on the image plane. Using the fluorescent image information generated by the algae, the algae and impurity particles in the field of view can be quickly distinguished.

[0029] In the transmission imaging mode, the laser 8 does not work, and the collimated light source 11 is located below the transparent carrier 7. First, the optical axes of the CMOS camera 1, the eyepiece 2, a bandpass filter 4, the beam splitter 5, the microscope objective 6, the transparent carrier 7 and the collimated light source 11 need to be aligned to complete the system calibration. When starting work, the light emitted by the collimated light source 11 is incident on the algae sample in parallel and passes through, and passes through the microscope objective 6, the beam splitter 5, the bandpass filter 4, and the eyepiece 2 in sequence until the CMOS camera 1 captures an image, and then the filter wheel 3 is rotated to switch to the next bandpass filter 4, so as to quickly obtain spectral information of different bands for spectral analysis. In this way, the transmitted light signal passes through the filter wheel 3 composed of six bandpass filters 4 with a full width at half maximum (FWHM) of 10nm in six different bands, and then the light converges to the CMOS camera 1, and the spectral information of spatial information can be obtained on the image plane.

[0030] After obtaining the fluorescence image and spectral information of the algae, the U-Net convolutional neural network can be used to achieve efficient recognition and accurate classification of the algae. The proposed U-Net model can combine the spatial-spectral feature information of the algae obtained by the above mode to achieve accurate recognition of the algae in the field of view.

[0031] Finally, the present invention should explain that: the above embodiments are detailed descriptions of the technical solutions of the present invention and are not limited to the aforementioned embodiments. Those skilled in the art should understand that modifications or replacements of the features and parameters in the aforementioned embodiments do not deviate from the spirit and scope of the technical solutions of the aforementioned embodiments.

Claims

1. A dual-mode microscopic hyperspectral imaging system for phytoplankton detection, used for detection and imaging of algae samples, characterized in that: It comprises a CMOS camera (1), an eyepiece (2), a filter wheel (3), a bandpass filter (4), a beam splitter (5), a microscope objective lens (6), a transparent carrier plate (7), a laser (8), a beam expander (9), a telephoto lens (10) and a collimated light source (11); The CMOS camera (1), the eyepiece (2), the filter wheel (3), the beam splitter (5), the microscope objective lens (6), the transparent carrier plate (7) and the collimated light source (11) are arranged in sequence from top to bottom; Six bandpass filters (4) are arranged on the filter wheel (3), the six bandpass filters (4) are located in the same plane, and the filter wheel (3) is used to drive the bandpass filters (4) to be located between the eyepiece (2) and the beam splitter (5); The algae sample is arranged on the transparent carrier sheet (7); The laser (8) is located on the side of the beam splitter (5), and a beam expander (9) and a telephoto lens (10) are arranged in sequence between the laser (8) and the beam splitter (5); the laser (8) is used to emit light that passes through the beam expander (9) and the telephoto lens (10), and then passes through the beam splitter (5), and part of the light passes through the microscope objective lens (6) to irradiate the algae sample to generate a fluorescent signal, and the fluorescent signal enters the microscope objective lens (6) and is imaged on the CMOS camera (1); The collimated light source (11) is used to emit light that passes through the algae sample to generate a transmission signal that enters the microscope objective lens (6) and is imaged on the CMOS camera (1).

2. The dual-mode microscopic hyperspectral imaging system for phytoplankton detection according to claim 1, characterized in that: The transparent carrier sheet (7) is a microfluidic chip.

3. The dual-mode microscopic hyperspectral imaging system for phytoplankton detection according to claim 1, characterized in that: The diameters of the six bandpass filters (4) are the same.

4. The dual-mode microscopic hyperspectral imaging system for phytoplankton detection according to claim 1, characterized in that: The six bandpass filters (4) are bandpass filters with a FWHM of 10 nm, and their wavelength bands are respectively: 630 nm, 650 nm, 610 nm, 490 nm, 590 nm and 530 nm.

5. The dual-mode microscopic hyperspectral imaging system for phytoplankton detection according to claim 1, characterized in that: The wavelength of the laser (8) is 532 nm.

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