Portable experimental device for rinsing brain slices

Abstract

The utility model discloses a portable brain slice rinsing experimental device, which relates to the field of experimental consumed material cleaning, and comprises a filter basket, a plurality of fan blades are uniformly arranged on the outer wall surface of the filter basket, the fan blades are made of rubber materials, a handle is arranged at the top of the filter basket, and the handle is arranged at the top of the filter basket and can be folded for storage. The brain slice cleaning device can be placed in a hole cavity of a six-hole plate or an experiment hole plate with other hole numbers, the containing space in the filter cylinder is used for containing a brain slice to be cleaned, the handle of the brain slice cleaning device is slightly rotated to drive the filter cylinder to rotate, the fan blades arranged on the outer wall face of the filter cylinder disturb cleaning liquid, and the cleaning liquid generates vortex to wash the brain slice. The brain slice cleaning device is lifted through the handle after cleaning is finished, brain slices and cleaning liquid are rapidly separated, a large number of brain slices can be rapidly cleaned, a shaking table does not need to be used for cleaning, the brain slices and the cleaning liquid can be rapidly separated, the brain slice cleaning device is convenient to carry, and experiment preparation time is greatly saved.

Description

Technical Field

[0001] This utility model relates to the field of cleaning laboratory consumables, and in particular to a convenient experimental device for rinsing brain slices. Background Technology

[0002] Frozen sections of rodent brains are a commonly used specimen in neuroscience research. The brain tissue or spinal cord, fixed in paraformaldehyde, is removed intact from the animal, dehydrated, and then sliced ​​into thin sections of varying thickness (16–40 μm) using a cryostat, depending on the experimental requirements. Generally, about 360 slices of varying sizes and shapes are obtained from a whole brain, and these are divided into six aliquots and stored in six-well plastic plates containing 0.01M PBS or cryoprotectant for subsequent experiments. After slicing, the frozen brain slices need to be washed three times with 0.01M PBS solution for 10 minutes each time to remove excess OCT gel.

[0003] Currently, brain slice washing is generally performed on a shaker and requires repeated washing in the washing solution. On the one hand, the need for a shaker places high demands on laboratory equipment; on the other hand, the washed brain slices are not easily transferred, and using a pipette will draw up the washing waste solution containing residual OCT gel, affecting the washing effect. Based on this, a convenient experimental device for rinsing brain slices is proposed. Utility Model Content

[0004] The present invention aims to provide a convenient experimental device for rinsing brain slices, in order to solve the following problems: on the one hand, the need for a shaker for rinsing, which places high demands on laboratory equipment; on the other hand, the brain slices are not easy to transfer after rinsing, and the use of a pipette will draw up the cleaning waste liquid containing residual OCT gel, which affects the cleaning effect.

[0005] To achieve the above objectives, this utility model provides the following technical solution:

[0006] A portable experimental apparatus for rinsing brain slices includes a filter basket with an upward-facing internal receiving space. The filter basket has multiple small holes arrayed on it, and multiple fan blades are evenly arranged on its outer wall. A handle is provided on the top of the filter basket, which can be folded and stored. The filter basket can be placed inside the cavity of an experimental well plate.

[0007] Furthermore, a suction cup is provided at the bottom of the filter basket, and the suction cup is rotatably connected to the bottom of the filter basket.

[0008] Furthermore, the fan blades are provided with water channels.

[0009] Furthermore, the fan blades are made of rubber.

[0010] Furthermore, the number of fan blades is two or more.

[0011] Furthermore, the handle is made of curved rigid steel wire that can be folded to the side inside the filter basket, and the filter basket is provided with protrusions to limit the position of the handle.

[0012] The principle and beneficial effects of this technical solution:

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] This invention can be placed inside the pores of a six-well plate or other well plates. The internal space of the filter cartridge is used to hold the brain slices to be cleaned. Gently turning the handle of this invention rotates the filter cartridge, and the fan blades on the outer wall of the filter cartridge agitate the cleaning solution, creating a vortex to rinse the brain slices, thus achieving the cleaning effect. After cleaning, the invention can be lifted by the handle to quickly separate the brain slices from the cleaning solution. This invention can quickly clean a large number of brain slices without the need for a shaker, and can quickly separate the brain slices from the cleaning solution. It is portable and convenient, greatly saving experimental preparation time. Attached Figure Description

[0015] Figure 1 A schematic diagram of a portable experimental apparatus for rinsing brain slices;

[0016] Figure 2 A schematic diagram of a portable experimental apparatus for rinsing brain slices;

[0017] Figure 3 A cross-sectional view of a convenient experimental apparatus for rinsing brain slices.

[0018] The reference numerals in the accompanying drawings include:

[0019] 1. Filter basket; 2. Small holes; 3. Fan blades; 4. Handle; 5. Suction cup; 6. Water passage; 7. Protrusions; 8. Six-hole plate. Detailed Implementation

[0020] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments:

[0021] like Figure 1-3 As shown, a portable experimental apparatus for rinsing brain slices includes a filter basket 1 with an upward-facing internal receiving space. Multiple small holes 2 are arrayed on the filter basket 1, and multiple blades 3, made of rubber, are evenly arranged on the outer wall of the filter basket 1. There are two or more blades 3; in this embodiment, there are three blades 3. A handle 4 is located on the top of the filter basket 1, allowing it to be folded and stored, so that the filter basket 1 can be placed inside the cavity of an experimental well plate.

[0022] As one embodiment of this utility model, a suction cup 5 is provided at the bottom of the filter basket 1, and the suction cup 5 is rotatably connected to the bottom of the filter basket 1. In use, the suction cup 5 is fixed to the bottom of the cavity of the experimental plate. Since the suction cup 5 is rotatably connected to the bottom of the filter basket 1, it will not affect the normal rotation of the filter basket 1. The fixing of the suction cup 5 also makes the rotation of the filter basket 1 more stable.

[0023] As one embodiment of this utility model, a water channel 6 is provided on the fan blade 3. The design of the water channel 6 prevents the cleaning fluid from overflowing from above the fan blade 3 into the cavity of the experimental plate when the fan blade 3 rotates.

[0024] As an embodiment of this utility model, the handle 4 is an arc-shaped hard steel wire that can be folded to the side inside the filter basket 1. The filter basket 1 is provided with protrusions 7 to limit the handle 4.

[0025] The specific implementation process is as follows:

[0026] This invention can be placed inside the pores of a six-well plate 8 or other experimental plates with multiple wells. The internal space of the filter cartridge is used to hold the brain slices to be cleaned. Gently turning the handle 4 of this invention causes the filter cartridge to rotate, and the fan blades 3 on the outer wall of the filter cartridge agitate the cleaning solution, creating a vortex to rinse the brain slices, thus achieving the cleaning effect. After cleaning, the invention can be lifted by the handle 4 to quickly separate the brain slices from the cleaning solution. This invention can quickly clean a large number of brain slices without the need for a shaker, and can quickly separate the brain slices from the cleaning solution. It is portable and convenient, greatly saving experimental preparation time.

[0027] The above descriptions are merely embodiments of this utility model. Commonly known technical solutions and / or characteristics are not described in detail here. It should be noted that those skilled in the art can make various modifications and improvements without departing from the technical solution of this utility model. These modifications and improvements should also be considered within the scope of protection of this utility model, and will not affect the effectiveness of the implementation of this utility model or the practicality of the patent. The scope of protection claimed in this application should be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. A portable experimental apparatus for rinsing brain slices, characterized in that: Includes a filter basket (1), the filter basket (1) has an upward-facing accommodating space inside, the filter basket (1) has multiple small holes (2) arrayed on it, the filter basket (1) has multiple fan blades (3) evenly arranged on its outer wall, the filter basket (1) has a handle (4) on its top, the handle (4) is located on the top of the filter basket (1) and can be folded and stored, the filter basket (1) can be placed in the cavity of the experimental well plate.

2. The portable experimental apparatus for rinsing brain slices according to claim 1, characterized in that: The bottom of the filter basket (1) is provided with a suction cup (5), and the suction cup (5) is rotatably connected to the bottom of the filter basket (1).

3. The portable experimental apparatus for rinsing brain slices according to claim 1, characterized in that: The fan blade (3) is provided with a water channel (6).

4. The portable experimental apparatus for rinsing brain slices according to claim 1, characterized in that: The fan blade (3) is made of rubber.

5. The portable experimental apparatus for rinsing brain slices according to claim 1, characterized in that: The number of fan blades (3) is two or more.

6. The portable experimental apparatus for rinsing brain slices according to claim 1, characterized in that: The handle (4) is an arc-shaped hard steel wire that can be folded to the side inside the filter basket (1). The filter basket (1) is provided with protrusions (7) to limit the handle (4).

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