Rat thrombus forming device

Abstract

The utility model discloses a rat thrombus forming device which comprises a blood collecting assembly and a thrombus forming assembly, the blood collecting assembly is used for collecting blood of a rat, and the blood collected by the blood collecting assembly is conveyed to the thrombus forming assembly to prepare thrombus; the blood sampling assembly comprises a bottom plate and a top plate, the top plate is located over the bottom plate, a vertical supporting rod is connected between the center of the bottom plate and the center of the top plate, the top plate can rotate around the supporting rod, a plurality of round holes are formed in the top plate in the annular direction, and blood collecting pipes are vertically placed in the round holes; a liquid outlet is formed in the bottom of the blood collecting pipe, a blood transfer pipe is connected to the liquid outlet, and the head end of the blood transfer pipe extends to the thrombus forming assembly; the thrombus forming assembly comprises a rectangular forming groove, a forming template made of rubber is fixed to the bottom of the forming groove, pits with honeycomb holes are densely distributed in the forming template, the diameter of each pit is 2-3 mm, and the depth of each pit is 2-3 mm.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the medical experiment equipment technical field, concretely relates to a rat thrombus forming device. BACKGROUND

[0002] In the study of the construction of chronic thromboembolic pulmonary hypertension (CTEPH) model, the most commonly used method is the autologous thrombus method. This method simulates the pulmonary hypertension caused by autologous thrombus formation. Specifically, first, autologous thrombus is injected through the jugular vein to induce thromboembolism in the pulmonary vessels, and then to cause the formation of pulmonary hypertension. In this method, the common operation steps include: using a capillary glass tube to collect autologous blood from the orbital vein of the rat, and placing it in a culture dish, and standing overnight to promote blood coagulation. Then, use normal saline to flush out the coagulated blood clots. Finally, use ophthalmic scissors or a scalpel to cut the blood clots into small pieces about 1 to 3 millimeters in size as thrombus material, ready for jugular vein injection. This operation process simulates the process of autologous thrombus formation, providing an effective experimental platform for the study of the pathophysiology of CTEPH.

[0003] The existing process of preparing thrombus has the following problems:

[0004] 1. Because the inner diameter of the capillary glass tube is small, the blood clots are easily restricted by the tube wall and are not easy to flush out smoothly, so it is more laborious to flush the blood clots out of the tube, and the process is more complicated.

[0005] 2. Since the thrombus needs to be cut into blocks in millimeters, it requires the operator to accurately control the size and length of each thrombus. Obviously, this process requires high operating skill and patience, so it takes a long time and often affects the overall progress of the experiment.

[0006] 3. Due to the limitations of human operation, the size and length of the thrombus are difficult to be completely consistent. If the length of the thrombus is too long, it may cause fatal complications in the rat during intravenous infusion; if the thrombus is too small, it may not effectively induce pulmonary thromboembolism, and thus the pulmonary hypertension model cannot be successfully established. Therefore, the size and length of the thrombus need to be strictly controlled to ensure the effectiveness of the experiment and the safety of the animals.

[0007] 4. Since the amount of thrombus needs to be controlled for each rat thrombus injection, but the thrombus is too small to count.

[0008] 5. The eye blood collection causes great harm to the rat, and the rat needs to be anesthetized for each blood collection, increasing the risk of death of the rat. UTILITY MODEL CONTENT

[0009] The utility model provides a rat thrombus forming device, it is in order to solve the existing thrombus preparation process, the coagulation blood is difficult to discharge in the capillary glass tube, and it is difficult to prepare the thrombus piece of uniform size.

[0010] For this purpose, the utility model adopts the following technical scheme:

[0011] A rat thrombus forming device, including blood sampling component and thrombus forming component, blood sampling component is used for collecting the blood of rat, and the blood collected by blood sampling component is transported to thrombus forming component to prepare thrombus;

[0012] The blood sampling component includes a bottom plate and a top plate, the top plate is located directly above the bottom plate, a vertical support rod is connected between the center of the bottom plate and the top plate, the top plate can rotate around the support rod, a plurality of circular holes are opened in the top plate along the ring direction, and a blood collection tube is vertically placed in each circular hole; The bottom of the blood collection tube is provided with a liquid outlet, a blood transfer tube is connected to the liquid outlet, and the head end of the blood transfer tube extends to the position of the thrombus forming component;

[0013] The thrombus forming component includes a rectangular forming groove, a forming template made of rubber is fixed at the bottom of the forming groove, the forming template is densely covered with concave pits with honeycomb holes, the diameter of the concave pits is 2-3mm, and the depth of the concave pits is 2-3mm.

[0014] Further, a scraper is also connected in the forming groove, and the scraper is used to scrape the blood on the forming template into the concave pits.

[0015] Further, the top of the left and right side plates of the forming groove is slidingly connected with a sliding block, and the scraper is obliquely arranged and connected between the two sliding blocks.

[0016] The utility model has the advantages of:

[0017] 1. Compared with the problem that the original thrombus coagulation is difficult to discharge in the capillary glass tube, the thrombus coagulation of the utility model is in the forming template, because the forming template is made of rubber, it is easy to extrude and deform, so that the coagulated thrombus is convenient to take out;

[0018] 2. The size of the concave pits on the forming template is fixed, the blood forms a standard size thrombus piece after coagulation, without secondary processing, the size and volume of the formed thrombus piece are consistent, the consistency is high, and the subsequent experiment is convenient;

[0019] 3. The device can be used for rat tail blood sampling, the blood sampling speed is fast, the harm degree to the rat is reduced, and the risk of rat death is reduced. DRAWINGS

[0020] Figure 1 It is the structure schematic diagram of the utility model;

[0021] Figure 2It is the structure schematic view of the forming groove of the utility model;

[0022] In the figure: 1 - bottom plate, 2 - top plate, 3 - blood collection tube, 4 - blood collection needle, 5 - blood transfer tube, 6 - forming groove, 7 - side plate, 8 - scraper, 9 - sliding block, 10 - base. DETAILED DESCRIPTION

[0023] The utility model is further described below in combination with the drawings and specific embodiments:

[0024] As Figure 1 And 2 As shown in the figure, a rat thrombus forming device includes a blood collection assembly and a thrombus forming assembly, the blood collection assembly is used for collecting blood of a rat, and the blood collected by the blood collection assembly is transported to the thrombus forming assembly to prepare a thrombus.

[0025] The blood collection assembly includes a bottom plate 1 and a top plate 2, the top plate 2 is located directly above the bottom plate 1, a vertical support rod is connected between the center of the bottom plate 1 and the top plate 2, the top plate 2 can rotate around the support rod, a plurality of circular holes are formed in the top plate 2 in a ring direction, and a blood collection tube 3 is vertically placed in the circular hole. The bottom of the blood collection tube 3 is provided with a liquid outlet, the blood transfer tube 5 is connected to the liquid outlet, and the head end of the blood transfer tube 5 extends to the position of the thrombus forming assembly. Blood is collected from the tail of the rat and is transported to the blood collection tube 3 through the blood collection needle 4.

[0026] The thrombus forming assembly includes a rectangular forming groove 6, the bottom of the forming groove 6 is fixed with a forming template made of rubber material, the forming template is densely covered with honeycomb hole pits, the diameter of the pits is 1-3 mm, and the depth of the pits is 1-3 mm. The forming template is made of thin rubber material and has strong deformation capacity, and the thrombus block in the pit can be conveniently taken out by extruding the rubber. In addition, in order to more conveniently extrude and take out the thrombus block in the pit, the forming groove 6 adopts a separated structure, the base 10 is independently formed, and the frame and the forming template are integrated; when in use, the frame and the forming template are installed on the base 10; when the separated blood block is extruded, the frame and the forming template are taken off from the base 10, the back of the forming template can be directly extruded, and the thrombus block can be conveniently extruded.

[0027] The scraper 8 is further connected in the forming groove 6, and the scraper 8 is used for scraping the blood on the forming template into the pit. The top of the left and right side plates of the forming groove 6 is slidingly connected with the sliding block 9, and the scraper 8 is obliquely arranged and connected between the two sliding blocks 9.

[0028] The use method of the utility model is as follows:

[0029] The blood is drained into the blood collection tube 3 by the blood collection needle 4. The device can be used to collect blood from one mouse or from multiple mice. The collected blood is transferred to the forming groove 6 by the blood transfer tube 5. The blood is scraped into the recess by the scraper 8. After the blood is coagulated, the base 10 is removed, the forming template is shaken and pressed, and the thrombus falls off.

Claims

1. A rat thrombus molding device, characterized by, The application relates to a blood sampling and thrombus forming device, which comprises a blood sampling component and a thrombus forming component. The blood sampling component comprises a bottom plate (1) and a top plate (2), the top plate (2) is located directly above the bottom plate (1), a vertical supporting rod is connected between the centers of the bottom plate (1) and the top plate (2), the top plate (2) can rotate around the supporting rod, a plurality of circular holes are arranged in the top plate (2) in a ring shape, and blood collection tubes (3) are vertically arranged in the circular holes; the bottom of each blood collection tube (3) is provided with a liquid outlet, a blood transfer tube (5) is connected to the liquid outlet, and the head end of the blood transfer tube (5) extends to the position of the thrombus forming component. The thrombus forming component comprises a rectangular forming groove (6), a forming template made of rubber is fixed to the bottom of the forming groove (6), the forming template is densely provided with a plurality of concaves in the form of honeycomb holes, the diameter of each concave is 1-3 mm, and the depth of each concave is 1-3 mm.

2. The rat thrombus shaping device of claim 1, wherein, A scraper (8) is further connected to the forming groove (6), and the scraper (8) is used for scraping the blood on the forming template into the concaves.

3. The rat thrombus shaping device of claim 1, wherein, The top portions of the left and right side plates of the forming groove (6) are slidably connected with sliding blocks (9), and the scraper (8) is arranged in an inclined mode and connected between the two sliding blocks (9).

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