Novel centrifugal tube for separating PBMC (peripheral blood mononuclear cells) from blood
By designing a transparent tube and a threaded cap, the problems of leakage and cell damage in existing centrifuge tubes were solved, enabling smooth operation and maintenance of cell viability.
Patent Information
- Application Number
- CN202423222366.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-26
- Publication Date
- 2025-12-23
- Estimated Expiration
- 2034-12-26
AI Technical Summary
Existing centrifuge tubes are prone to leakage when injecting and removing blood, and the threaded caps are difficult to unscrew under negative pressure, affecting the effectiveness of use. Furthermore, cells are easily attached and damaged during centrifugation.
A novel centrifuge tube was designed, comprising a transparent tube body, a conical tube, a threaded flared sleeve, a threaded cap, and a rubber stopper. The threaded flared sleeve increases the inner diameter at the top, the threaded cap has a through hole and a rubber stopper to maintain consistent air pressure, the inner smooth layer reduces cell adhesion, and the threaded cap reinforcement strip enhances stability.
It enables smoother blood injection and retrieval, avoids the effects of negative pressure, maintains cell activity, and has a good sealing effect, making it less prone to leakage.
Smart Images
Figure CN223697807U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of blood centrifuge tube technology, and more specifically, to a novel centrifuge tube for separating blood PBMCs. Background Technology
[0002] In biomedical research and clinical applications, peripheral blood mononuclear cells (PBMCs) are an important type of immune cell. Because they contain key cell types such as lymphocytes and monocytes, they play a crucial role in research in multiple fields such as immunology, oncology, and genetics. The isolation and purification of PBMCs is a fundamental step in related research, and their quality and purity directly affect the accuracy and reliability of subsequent experimental results.
[0003] Traditional PBMC separation methods mainly rely on density gradient centrifugation. This method utilizes the density differences of different cell components to separate cells into layers through centrifugation. In this process, the centrifuge tube, as the container for carrying blood samples and separation media, has a significant impact on the separation effect due to its design and material.
[0004] Existing centrifuge tubes have a consistent inner diameter from top to bottom, resulting in a relatively small inner diameter at the tube opening. This makes leakage prone to occur during blood injection and extraction. Furthermore, while the threaded cap provides some sealing, it lacks a permeable component to allow air to pass through, hindering the maintenance of consistent internal and external pressure. When the tube is under negative pressure, the cap becomes difficult to unscrew, affecting usability and causing inconvenience to the user. Therefore, we propose a novel centrifuge tube for blood separation of PBMCs. Utility Model Content
[0005] The purpose of this invention is to provide a novel centrifuge tube for separating blood PBMCs, in order to solve the defects mentioned in the background art.
[0006] To achieve the above objectives, this utility model provides the following technical solution:
[0007] A novel centrifuge tube for blood separation PBMCs includes a transparent tube body with a conical core integrally formed at the bottom. A threaded flared sleeve is fixedly installed at the top of the transparent tube body, and a threaded cap is threadedly connected to the threaded flared sleeve. Reinforcing strips are fixedly installed on the top surface and annular side surface of the threaded cap. A through hole communicating with the outside is provided on the top plate of the threaded cap, and a rubber plug is fixedly installed in the through hole. End fixing rings are fixedly installed at both ends of the rubber plug, and the two end fixing rings are respectively fixedly installed on the inner and outer surfaces of the top plate of the threaded cap.
[0008] Preferably, the conical tube is in the shape of a hollow frustum, and the inner diameter of the conical tube decreases from top to bottom.
[0009] Preferably, the surface of the transparent tube is provided with scale lines arranged along the height direction of the transparent tube, and the top inner diameter of the threaded flared sleeve is larger than the inner diameter of the transparent tube.
[0010] Preferably, the inner surface of the threaded flared sleeve is provided with an inclined surface, which is inclined downward at 45°~60°.
[0011] Preferably, an inner smooth layer is provided on the inner surface of both the transparent tube and the threaded flared sleeve, and anti-slip stripes are provided on the outer annular side of the threaded cap.
[0012] Preferably, an inner sealing gasket is fixedly installed on the top wall of the threaded cover, and the inner sealing gasket abuts against the top surface of the threaded flared sleeve.
[0013] Preferably, a cardboard limiting ring is fixedly installed on the smooth end of the bottom cylinder of the threaded flared sleeve, and the threaded cap abuts against the upper surface of the cardboard limiting ring.
[0014] Preferably, the reinforcing strip has a cross-shaped cross section, and the reinforcing strip and the threaded cap are integrally formed.
[0015] Compared with the prior art, the beneficial effects of this utility model are:
[0016] 1. This utility model increases the inner diameter of the top of the tube by setting a threaded flared sleeve, making it easier to inject and remove blood. In addition, the threaded cap with a rubber stopper allows an external needle to penetrate the rubber stopper when needed. This ensures that the air pressure inside and outside the transparent tube is consistent, avoiding negative pressure that would make it difficult to unscrew the cap. This achieves the effect of allowing air to pass through and making injection and liquid removal smoother.
[0017] 2. The inner smoothing layer of this utility model makes its inner surface smoother, thereby reducing cell adhesion and damage during centrifugation and maintaining the activity of PBMCs. In addition, the reinforcing strip makes the threaded cap more secure and less prone to deformation. Attached Figure Description
[0018] Figure 1 This is a schematic diagram of the overall structure of this utility model.
[0019] Figure 2 This is a schematic diagram of the exploded structure of this utility model.
[0020] Figure 3This is one of the structural schematic diagrams of this utility model.
[0021] Figure 4 This is the second partial structural schematic diagram of this utility model.
[0022] The meanings of the labels in the diagram are as follows:
[0023] 1. Transparent tube body; 10. Conical tube; 11. Scale lines; 12. Threaded flared sleeve; 121. Bevel; 13. Inner smooth layer; 14. Hard paper limiting ring;
[0024] 2. Threaded cap; 20. Anti-slip stripe; 21. Reinforcing strip; 22. Inner sealing gasket; 23. Through hole; 24. Rubber plug; 25. End fixing ring. Detailed Implementation
[0025] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0026] Please see Figures 1-4 This utility model provides a technical solution: a novel centrifuge tube for separating blood PBMCs, including a transparent tube body 1, with a conical tube 10 integrally formed at the bottom of the transparent tube body 1. The conical tube 10 is in the shape of a hollow frustum, and the inner diameter of the conical tube 10 decreases from top to bottom, which helps to improve the stratification effect of blood samples during centrifugation.
[0027] Specifically, a threaded flared sleeve 12 is fixedly installed at the top of the transparent tube 1, and a threaded cap 2 is threadedly connected to the threaded flared sleeve 12. The inner diameter of the top of the threaded flared sleeve 12 is larger than the inner diameter of the transparent tube 1, making the opening at the top larger, which facilitates the injection and liquid retrieval operations.
[0028] Specifically, a reinforcing strip 21 is fixedly installed on the top surface and the annular side of the threaded cap 2. The cross section of the reinforcing strip 21 is cross-shaped. The reinforcing strip 21 and the threaded cap 2 are integrally formed, making the structure of the threaded cap 2 more robust and stable, and preventing deformation under stress.
[0029] In this embodiment, the top plate of the threaded cap 2 is provided with a through hole 23 that communicates with the outside. A rubber plug 24 is fixedly installed in the through hole 23. Both ends of the rubber plug 24 are fixedly installed with end fixing rings 25. The two end fixing rings 25 are respectively fixedly installed on the inner and outer surfaces of the top plate of the threaded cap 2. By setting the end fixing rings 25, the fixed contact area between the plug and the threaded cap 2 can be increased, making the fixation more secure. At the same time, by setting the rubber plug 24, when necessary, a needle can be used to penetrate the rubber plug 24 to ensure that the internal and external air pressure inside the transparent tube 1 is consistent, and to avoid negative pressure inside the transparent tube 1 affecting the normal unscrewing of the threaded cap 2.
[0030] In this embodiment, a scale line 11 is provided on the surface of the transparent tube 1 along the height direction of the transparent tube 1, which facilitates accurate control of the amount of blood sample and separation medium added according to the scale line 11.
[0031] Specifically, the inner surface of the threaded flared sleeve 12 is provided with a bevel 121, which is inclined downward at 45°~60°, making it easier to add liquid along the bevel 121.
[0032] Furthermore, an inner smooth layer 13 is provided on the inner surface of both the transparent tube 1 and the threaded flared sleeve 12. The inner smooth layer 13 can be made by grinding or other processes to make its inner surface smoother, so as to reduce cell adhesion and damage during centrifugation and thus maintain the activity of PBMCs.
[0033] In addition, anti-slip stripes 20 are provided on the outer annular side of the threaded cap 2. The anti-slip stripes 20 can increase the friction and make it easier to rotate the threaded cap 2.
[0034] It is worth noting that an inner sealing gasket 22 is fixedly installed on the top wall of the threaded cover 2. The inner sealing gasket 22 abuts against the top surface of the threaded flared sleeve 12. The inner sealing gasket 22 can improve the sealing effect and make it less likely to leak.
[0035] It is worth noting that a cardboard limiting ring 14 is fixedly installed on the smooth end of the bottom cylinder of the threaded flared sleeve 12. The threaded cover 2 rests against the upper surface of the cardboard limiting ring 14 to limit the tightening depth of the threaded cover 2 and prevent the threaded cover 2 from being over-tightened and damaged.
[0036] In use, the novel centrifuge tube for blood separation of PBMCs is made by adding blood and the corresponding drug solution into the transparent tube body 1 through the threaded flared sleeve 12. The amount of blood sample and separation medium added is accurately controlled according to the scale line 11. After adding the solution, the threaded cap 2 is tightened on the threaded flared sleeve 12, and then the transparent tube body 1 is placed in an external centrifuge for centrifugation. After centrifugation, the transparent tube body 1 is removed, the threaded cap 2 is unscrewed, and then the liquid is collected.
[0037] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely preferred examples and are not intended to limit the utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed utility model. The scope of protection of this utility model is defined by the appended claims and their equivalents.
Claims
1. A novel centrifuge tube for separating blood PBMCs, comprising a transparent tube body (1), characterized in that: The bottom of the transparent tube (1) is integrally formed with a conical tube (10). The top of the transparent tube (1) is fixedly installed with a threaded flared sleeve (12). A threaded cap (2) is threadedly connected to the threaded flared sleeve (12). A reinforcing strip (21) is fixedly installed on the top surface and the annular side surface of the threaded cap (2). A through hole (23) communicating with the outside is provided on the top plate of the threaded cap (2). A rubber plug (24) is fixedly installed in the through hole (23). An end fixing ring (25) is fixedly installed at both ends of the rubber plug (24). The two end fixing rings (25) are respectively fixedly installed on the inner and outer surfaces of the top plate of the threaded cap (2).
2. The novel centrifuge tube for blood separation PBMCs according to claim 1, characterized in that: The conical tube (10) is in the shape of a hollow frustum, and the inner diameter of the conical tube (10) decreases from top to bottom.
3. The novel centrifuge tube for blood separation PBMCs according to claim 1, characterized in that: The transparent tube (1) has a scale line (11) set along the height direction of the transparent tube (1) on its surface, and the top inner diameter of the threaded flared sleeve (12) is larger than the inner diameter of the transparent tube (1).
4. The novel centrifuge tube for blood separation PBMCs according to claim 1, characterized in that: The inner surface of the threaded flared sleeve (12) is provided with an inclined surface (121), which is inclined downward at 45°~60°.
5. The novel centrifuge tube for blood separation PBMCs according to claim 1, characterized in that: The inner surfaces of the transparent tube (1) and the threaded flared sleeve (12) are provided with an inner smooth layer (13), and the outer annular side of the threaded cap (2) is provided with anti-slip stripes (20).
6. The novel centrifuge tube for blood separation PBMCs according to claim 1, characterized in that: An inner sealing gasket (22) is fixedly installed on the top wall of the threaded cover (2), and the inner sealing gasket (22) abuts against the top surface of the threaded flared sleeve (12).
7. The novel centrifuge tube for blood separation PBMCs according to claim 1, characterized in that: A cardboard limiting ring (14) is fixedly installed on the smooth end cylinder of the bottom of the threaded flared sleeve (12), and the threaded cap (2) abuts against the upper surface of the cardboard limiting ring (14).
8. The novel centrifuge tube for blood separation PBMCs according to claim 1, characterized in that: The cross section of the reinforcing strip (21) is cross-shaped, and the reinforcing strip (21) and the threaded cap (2) are integrally formed.