Ultrasonic DNA breaking instrument

Abstract

The utility model discloses an ultrasonic DNA (deoxyribonucleic acid) breaking instrument which comprises an outer shell, a water bath basin assembly is fixedly mounted in the outer shell, a rotating support is mounted in the water bath basin assembly, and a driver for driving the rotating support to rotate is mounted above the water bath basin assembly; the rotating support comprises a hand screw and a bi-pass stud which are connected through threads, and an inserting groove is formed in the hand screw. A supporting plate is installed at the connecting position of the hand screw and the two-way stud, a pressing plate is connected to a two-way stud body in a threaded mode, a hole plate is fixedly installed at the bottom end of the two-way stud, and bottom feet are fixedly installed at the bottom of the hole plate. The ultrasonic DNA breaking instrument provided by the utility model can be directly taken and placed, test tubes can be conveniently taken and placed, a transmission route can be simplified, transmission loss can be reduced, and the ultrasonic DNA breaking instrument can have two power supply modes so as to improve the application range.

Description

TECHNICAL FIELD

[0001] The utility model relates to an ultrasonic DNA breaking instrument belongs to experimental equipment technical field. BACKGROUND

[0002] The principle of the non-contact ultrasonic DNA breaking instrument is based on the mechanical vibration and cavitation effect generated by ultrasonic waves. The high-frequency electrical signal generated by the ultrasonic generator is converted into mechanical vibration by the transducer and acts on the liquid containing the DNA sample. Under the action of ultrasonic waves, small bubbles are generated in the liquid medium. These bubbles grow rapidly and break, generating strong shock waves and microjets that can penetrate cell membranes and nucleic acid molecules, accurately breaking DNA molecular chains. It can be applied to fields such as bacterial cell disruption, protein extraction, second-generation sequencing sample DNA fragmentation, immunoprecipitation experiments, mold spore disruption, emulsification, homogenization, accelerated dissolution, catalytic reactions, etc. It plays an important role in DNA sample processing in molecular biology research.

[0003] For example, patent number CN202323275485.9 discloses a sample adapter driving unit and a non-contact ultrasonic DNA breaking instrument, and patent number CN109266545A discloses an ultrasonic DNA breaking instrument. The technical solutions disclosed in the above patent documents still have the following technical problems according to practice:

[0004] 1. The driving unit drives the rotation of the test tube through a gear transmission, the transmission route is long, and there is a problem of high transmission loss;

[0005] 2. The driving unit needs to be directly connected to an external power supply. When there is no external power supply, it cannot be powered, and DNA breaking processing cannot be performed, resulting in limited application range;

[0006] 3. The test tube mounting rack cannot be placed vertically due to structural limitations, and must be operated with both hands during test tube taking and placing, resulting in inconvenient operation.

[0007] As can be seen from the above, the prior art has obvious inconvenience and defects in actual use, and therefore needs to be improved. UTILITY MODEL CONTENTS

[0008] The utility model provides an ultrasonic DNA breaking instrument, which can be directly taken and placed, facilitates test tube taking and placing operation, can simplify the transmission route and reduce transmission loss, and can have two power supply modes to improve the application range.

[0009] To solve the above technical problems, the utility model adopts the following technical solutions:

[0010] The utility model provides an ultrasonic DNA breaking instrument, including the shell, the water bath basin subassembly is fixedly installed inside the shell, the rotating support is installed inside the water bath basin subassembly, the driver that drives rotating support rotates is installed on the water bath basin subassembly top,

[0011] Rotating support includes by screwing hand screw and double -threaded stud, and the slot is arranged in the inside of hand screw, and the connecting portion of hand screw and double -threaded stud is equipped with the supporting plate, and the double -threaded stud main body is equipped with the pressing plate by screwing, and the double -threaded stud bottom end is fixedly installed with the hole plate, and the hole plate bottom is fixedly installed with the foot.

[0012] Further, the top of the shell is provided with a flip cover, and the side of the flip cover is provided with a touch screen.

[0013] Further, the water bath basin subassembly is located directly below the flip cover.

[0014] Further, a platform is installed between the driver and the water bath basin subassembly, the platform is fixedly installed above the water bath basin subassembly, the platform is provided with a through hole inside, the platform is used for supporting the rotating support inside, and the platform is used for supporting the driver on the top.

[0015] Further, the water bath basin subassembly includes a water bath basin, the water bath basin is provided with an opening at the top, the opening end of the water bath basin is fixedly connected to the bottom of the water bath basin mounting plate, and the periphery of the water bath basin mounting plate is fixedly connected to the inner wall of the shell.

[0016] Further, the water bath basin is provided with an ultrasonic transducer around and at the bottom.

[0017] Further, the driver includes a bottom plate, a motor is fixedly installed on the bottom plate, the output shaft of the motor is arranged through the bottom plate in the vertical direction, a protective cover is buckled outside the motor, and a lithium battery is installed inside the protective cover.

[0018] Further, the top of the protective cover is provided with a power switch, and the side is provided with a TYPC charging port.

[0019] Further, the feet are distributed in a circle.

[0020] Further, a water inlet and outlet joint is installed on the outer wall of the shell.

[0021] After the above technical scheme is adopted, compared with the prior art, the following advantages are obtained:

[0022] The rotating support and the installed test tube are placed in the water bath basin in the longitudinal direction, and are directly taken and placed; the foot is fixedly installed at the bottom of the hole plate, the rotating support can be placed longitudinally by setting the foot, and the test tube can be taken and placed by one hand;

[0023] The output shaft of the motor is directly inserted into the slot at the top of the rotating support, which solves the problem of long transmission route and high transmission loss in the prior art;

[0024] The driver has a built-in lithium battery to power the motor. The motor has two power supply modes: direct power supply and indirect power supply. The motor can be directly powered by an external power source or powered by the lithium battery to improve its applicability.

[0025] The present invention will now be described in detail with reference to the accompanying drawings and embodiments. Attached Figure Description

[0026] Figure 1 This is a schematic diagram of the structure of this utility model;

[0027] Figure 2 This is a schematic diagram of the internal structure of this utility model;

[0028] Figure 3 This is a cross-sectional schematic diagram of the present invention along its longitudinal direction;

[0029] Figure 4 This is a schematic diagram of the structure of the water bath assembly in this utility model;

[0030] Figure 5 This is a front view of the rotating bracket structure in this utility model;

[0031] Figure 6 This is a three-dimensional view of the rotating bracket in this utility model.

[0032] In the diagram, 1-outer shell, 2-flip cover, 3-touch screen; 4-water bath assembly, 41-water bath, 42-water bath mounting plate, 43-ultrasonic transducer; 5-driver, 51-protective cover, 52-lithium battery, 53-motor, 54-base plate; 6-rotating bracket, 61-hand screw, 62-double stud, 63-support plate, 64-pressure plate, 65-perforated plate, 66-foot; 7-platform. Detailed Implementation

[0033] To provide a clearer understanding of the technical features, objectives, and effects of this utility model, the specific embodiments of this utility model are now described with reference to the accompanying drawings.

[0034] like Figures 1-6 As shown in the figure, this utility model provides an ultrasonic DNA breaking device, including a housing 1, a flip cover 2 on the top of the housing 1, and a touch screen 3 on the side of the flip cover 2.

[0035] A water bath assembly 4 is fixedly installed inside the outer shell 1. The water bath assembly 4 is located directly below the flip cover 2. A rotating bracket 6 is installed inside the water bath assembly 4. A driver 5 that drives the rotating bracket 6 to rotate is installed above the water bath assembly 4. A platform 7 is installed between the driver 5 and the water bath assembly 4.

[0036] The platform 7 is fixedly installed above the water bath assembly 4. The platform 7 has a through hole inside, which is used to support the rotating bracket 6. The top of the platform 7 is used to support the driver 5.

[0037] The water bath assembly 4 includes a water bath 41 with an opening at the top. The opening end of the water bath 41 is fixed to the bottom of the water bath mounting plate 42, and the periphery of the water bath mounting plate 42 is fixed to the inner wall of the outer shell 1.

[0038] Ultrasonic transducers 43 are installed around the water bath 41 and at the bottom. The ultrasonic transducers 43 are used to generate high-frequency vibrations.

[0039] The driver 5 includes a base plate 54, on which a motor 53 is fixedly mounted. The output shaft of the motor 53 passes through the base plate 54 in a vertical direction. A protective cover 51 is fastened to the outside of the motor 53. A lithium battery 52 is installed inside the protective cover 51, and the lithium battery 52 supplies power to the motor 53.

[0040] The protective cover 51 has a power switch on the top and a TYPC charging port on the side. The motor 53 has two power supply modes: direct power supply and indirect power supply. The motor 53 can be directly powered by an external power source or powered by a lithium battery 52.

[0041] The rotating bracket 6 includes a hand screw 61 and a double stud 62 connected by threads. The hand screw 61 has a slot inside for longitudinal insertion of the output shaft of the motor 53.

[0042] A support plate 63 is installed at the connection between the hand-tightening screw 61 and the double-through stud 62, and the support plate 63 can be placed inside the platform 7.

[0043] A pressure plate 64 is threaded onto the main body of the double stud 62. The pressure plate 64 is used to press the open end of the test tube.

[0044] A perforated plate 65 is fixedly installed at the bottom of the double stud 62, and the perforated plate 65 has multiple test tube placement holes.

[0045] The bottom of the orifice plate 65 is fixedly installed with three feet 66 arranged in a circle. By setting the feet 66, the rotating bracket 6 can be placed vertically, and test tubes can be picked up and put in with one hand.

[0046] The outer wall of the outer casing 1 is equipped with a water inlet / outlet connector, through which circulating cooling water is introduced.

[0047] The specific working principle of this utility model is as follows:

[0048] When performing DNA fragmentation on a sample, the sample is first loaded into test tubes, and each test tube is installed in the well plate 65. The pressure plate 64 is tightened to press the open end of the test tube. The rotating support 6 and the assembled test tubes are placed longitudinally in the water bath 41, and the support plate 63 is placed on the platform 7. The driver 5 is installed longitudinally, so that the output shaft of the motor 53 extends into the hand screw 61. While the motor 53 drives the rotating support 6, the ultrasonic transducer 43 emits high-frequency vibrations, which act on the DNA-containing sample to perform fragmentation.

[0049] The above description provides examples of the preferred embodiments of this utility model. Any aspects not detailed herein are common knowledge to those skilled in the art. The scope of protection of this utility model is determined by the claims. Any equivalent modifications based on the technical teachings of this utility model are also within the scope of protection of this utility model.

Claims

1. An ultrasonic DNA fragmentation device, characterized in that: Includes an outer shell (1), inside which a water bath assembly (4) is fixedly installed, inside which a rotating bracket (6) is installed, and above the water bath assembly (4) a driver (5) is installed to drive the rotating bracket (6) to rotate. The rotating bracket (6) includes a hand screw (61) and a double stud (62) connected by threads. The hand screw (61) has a slot inside. A support plate (63) is installed at the connection between the hand screw (61) and the double stud (62). A pressure plate (64) is threadedly connected to the body of the double stud (62). A perforated plate (65) is fixedly installed at the bottom of the double stud (62). A foot (66) is fixedly installed at the bottom of the perforated plate (65).

2. The ultrasonic DNA disruptor as described in claim 1, characterized in that: The outer casing (1) has a flip cover (2) on the top and a touch screen (3) on the side of the flip cover (2).

3. The ultrasonic DNA disruptor as described in claim 2, characterized in that: The water bath assembly (4) is located directly below the flip cover (2).

4. The ultrasonic DNA disruptor as described in claim 1, characterized in that: A platform (7) is installed between the driver (5) and the water bath assembly (4). The platform (7) is fixedly installed above the water bath assembly (4). The platform (7) has a through hole inside. The inside of the platform (7) is used to support the rotating bracket (6). The top of the platform (7) is used to support the driver (5).

5. The ultrasonic DNA disruptor as described in claim 1, characterized in that: The water bath assembly (4) includes a water bath (41), with an opening at the top. The opening end of the water bath (41) is fixed to the bottom of the water bath mounting plate (42), and the periphery of the water bath mounting plate (42) is fixed to the inner wall of the outer shell (1).

6. The ultrasonic DNA disruptor as described in claim 5, characterized in that: Ultrasonic transducers (43) are installed around the water bath (41) and at the bottom.

7. The ultrasonic DNA disruptor as described in claim 1, characterized in that: The driver (5) includes a base plate (54), on which a motor (53) is fixedly mounted. The output shaft of the motor (53) passes through the base plate (54) in a vertical direction. A protective cover (51) is fastened to the outside of the motor (53), and a lithium battery (52) is installed inside the protective cover (51).

8. The ultrasonic DNA disruptor as described in claim 7, characterized in that: The protective cover (51) has a power switch on the top and a TYPC charging port on the side.

9. The ultrasonic DNA disruptor as described in claim 1, characterized in that: The base (66) is distributed in a circular pattern.

10. The ultrasonic DNA disruptor as described in claim 1, characterized in that: The outer wall of the outer shell (1) is equipped with water inlet and outlet connectors.

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