Digital microfluidic system containing quartz crystal microbalance sensor

Abstract

The utility model discloses a digital micro-fluidic system containing a quartz crystal microbalance sensor. The digital micro-fluidic system comprises an integrated system and a main body, the integrated system comprises a constant temperature system, a microfluidic system, a quartz crystal microbalance system, a control system and a data processing system, automatic control of subsystems of the instrument is realized by using an embedded system, and integrated control of the subsystems is completed by combining system programming. A main body is designed to surround a micro-fluid channel of a micro-fluidic chip and a layout of a quartz crystal sensor, the QCM sensor is connected with the micro-fluid channel of the micro-fluidic chip, it is ensured that fluid can be in contact with the sensor through the channel, fluid flowing is accurately controlled through a peristaltic pump and the micro-fluid channel, and a sample pool and a waste liquid pool are arranged; the container is used for storing samples and collecting waste liquid; mechanical support design is carried out on an instrument support structure, and internal structure protection design is carried out on each control and interface unit.

Description

TECHNICAL FIELD

[0001] The utility model relates to biological detection technical field especially, one kind contains digital microfluidics system of quartz crystal microbalance sensing. BACKGROUND

[0002] Digital quartz crystal microbalance technology solves the problems of large sample volume, slow analysis speed, and limitations in processing small mass changes in the traditional quartz crystal microbalance technology during sample processing and analysis with the characteristics of miniaturization, integration, and high throughput. Digital quartz crystal microbalance technology can distinguish the influence of mass and viscoelasticity on frequency change, thus having higher sensitivity and accuracy. In addition, by simultaneously measuring frequency change (Δf) and dissipation change (ΔD), real-time monitoring of sample mass change and viscoelastic properties, etc., more information about the sample is provided, which can play a greater value in biological molecular interaction, cell adhesion, polymer viscoelasticity analysis, etc.

[0003] The digital microfluidics system instrument containing quartz crystal microbalance sensing can provide multifunctional, high-sensitivity, and real-time monitoring analysis tools for the fields of biosensing and analytical chemistry, expanding the application range in the fields of biotechnology, medical devices, energy cleaning, food and pharmaceuticals, etc. Through precise control of sample flow rate and temperature by the microfluidic system, the accuracy of digital quartz crystal microbalance sample processing, as well as the efficiency and flexibility of detection, are improved, while the waste of scarce samples can be avoided. The integration of microfluidic technology can further improve the sensitivity of digital quartz crystal microbalance, enabling the system to detect weaker signals. The instrument can be suitable for various complex liquid environments, and by integrating microfluidic technology with digital quartz crystal microbalance, complex sensing and detection tasks can be completed on a compact platform.

[0004] Digital quartz crystal microbalance mainly consists of a constant temperature system, a microfluidic system, a quartz crystal microbalance system, a control system, a data processing system, etc. The microfluidic system mainly consists of a microfluidic chip and a microfluidic channel. The quartz crystal microbalance system mainly consists of a quartz crystal sensor and an oscillator circuit system. The control system is mainly used to control sample sampling in the sample area, control fluid flow in the microfluidic chip, and collect signals from the QCM sensor. The data processing system is used to analyze the signals from the quartz crystal sensor and the data collected from the microfluidic chip, and to complete detection, data collection, and analysis.

[0005] In view of the above-mentioned defects, the present design person actively researches and innovates to create a digital microfluidics system containing quartz crystal microbalance sensing, which has more industrial utilization value. UTILITY MODEL CONTENT

[0006] The utility model discloses a digital micro -fluidic system of quartz crystal micro -balance sensing to solve above -mentioned technical problem, and the purpose is to provide a kind of digital micro -fluidic system of quartz crystal micro -balance sensing.

[0007] A kind of digital micro -fluidic system of quartz crystal micro -balance sensing of the utility model is composed of integrated system and main part;

[0008] Integrated system includes control system, data processing system, quartz crystal micro -balance system, constant temperature system and micro -fluidic system, and control system is connected with data processing system by electric signal, and data control system is connected with quartz crystal micro -balance system, constant temperature system and micro -fluidic system by electric signal;

[0009] Main part includes instrument structure, power module and pipeline and connecting piece, power module, control system, data processing system, quartz crystal micro -balance system, constant temperature system and micro -fluidic system are fixed in instrument structure, power module is powered for control system, data processing system, quartz crystal micro -balance system, constant temperature system and micro -fluidic system, and pipeline and connecting piece are used to connect the intercommunication of power module and control system, data processing system, quartz crystal micro -balance system, constant temperature system and micro -fluidic system.

[0010] Further, the quartz crystal micro -balance system is installed with the chip memory that can eject, chip is placed in chip memory, and chip memory resets and makes chip link with quartz crystal sensor, and the press memory switch for controlling chip memory ejecting is installed on instrument structure, and handle is installed on the outer end surface of chip memory.

[0011] Further, the constant temperature system includes a support that places sample reagent and waste liquid bottle, and the thermostat in instrument structure provides temperature.

[0012] Further, micro -fluidic system controls peristaltic pump and sampler and transports reagent to micro -fluidic chip, and detection analysis subsystem in data processing system detects, data acquisition and analysis reagent reaction on micro -fluidic chip.

[0013] Further, quartz crystal micro -balance system sends instruction by control system, so that micro -fluidic system passes into chip memory by micro -flow pipeline, and quartz crystal sensor records chip change, and exports record data to data processing system.

[0014] Further, instrument structure is installed with the LED display screen of data display.

[0015] Further, instrument structure further includes backboard and side plate.

[0016] By the above scheme, the utility model at least has following advantages:

[0017] (1) Through microfluidic technology, the sample processing efficiency of QCM is improved, rapid processing and analysis of samples are realized;

[0018] (2) The accurate control of sample solution by microfluidic technology and the high sensitivity detection of QCM improve the accuracy of analysis;

[0019] (3) Through integrated design, it is convenient to carry and operate, and is suitable for on-site detection and laboratory research.

[0020] The above description is only a summary of the technical scheme of the present application, in order to more clearly understand the technical means of the present application, and can be implemented according to the content of the specification, the following will be described in detail with the preferred embodiment of the present application and the accompanying drawings. BRIEF DESCRIPTION OF DRAWINGS

[0021] In order to more clearly illustrate the technical scheme of the present application, the following will briefly introduce the drawings needed in the embodiment, it should be understood that the following drawings only show some embodiments of the present application, therefore should not be regarded as limiting the scope, for those skilled in the art, without creative labor, other related drawings can also be obtained according to these drawings.

[0022] Figure 1 It is the appearance structure schematic diagram of the present application;

[0023] Figure 2 It is the operation system schematic diagram of the present application;

[0024] Figure 3 It is the main body structure schematic diagram of the present application;

[0025] Figure 4 It is the main board circuit schematic diagram of the present application;

[0026] Figure 5 It is the control system schematic diagram of the present application;

[0027] Figure 6 It is the quartz crystal microbalance system schematic diagram of the present application;

[0028] In the figure: 1, integrated system; 2, main body; 11 control system; 12 data processing system; 13 quartz crystal microbalance system; 14, switch; 15, constant temperature system; 16, microfluidic system; 21, instrument structure; 22, power module; 23, pipeline and connecting piece; 91, waste liquid bottle; 111, sampling machine; 112, motor; 113, sensor; 119, system circuit; 121, detection and analysis subsystem; 129, LED display screen; 131, memory switch; 132, chip memory; 133, handle; 134, quartz crystal sensor; 135, chip; 139, microflow pipeline; 151, sample reagent; 152, thermostat, 161, peristaltic pump; 162, microfluidic chip; 213, back plate; 214, side plate. DETAILED DESCRIPTION

[0029] The specific embodiments of the present application will be further described in detail below with reference to the accompanying drawings and examples. The following examples are used to illustrate the present application, but not to limit the scope of the present application.

[0030] As shown in Figure 1 , Figure 2 , Figure 3 , a digital microfluidic system containing quartz crystal microbalance sensing includes integrated system 1 and main body 2, integrated system 1 includes control system 11, data processing system 12, quartz crystal microbalance system 13, constant temperature system 15, microfluidic system 16, main body 2 includes instrument structure 21, power module 22, pipeline and connecting piece 23.

[0031] Quartz crystal microbalance system 13 in integrated system 1, press memory switch 131 pop chip memory 132, place chip 135, push handle 133 make chip memory 132 home and quartz crystal sensor 134 link.

[0032] Constant temperature system 15 in integrated system 1, place sample reagent 151, set thermostat 152 temperature in control system 11.

[0033] Microfluidic system 16 in integrated system 1, press control system switch 14, send instructions to sampling machine 111 sampling in control system 11, through peristaltic pump 161 transmission sample liquid to microfluidic chip 162, detection and analysis subsystem 121 in data processing system 12 detects the reaction on the microfluidic chip, data acquisition and analysis.

[0034] The microfluidic system 16 in the integrated system 1 sends instructions to the sampler 111 to sample under the control of the control system 11, and transmits sample liquid to the microfluidic chip 162 through the peristaltic pump 161, the detection and analysis subsystem 121 in the data processing system 12 detects, collects data and analyzes the reaction on the microfluidic chip, and after the analysis is completed, the waste liquid is automatically transported to the waste liquid bottle 91.

[0035] The quartz crystal microbalance system 13 in the integrated system 1 sends instructions to the microfluidic system 16 to transmit sample liquid into the chip storage 132 through the microfluidic channel 139 under the control of the control system 11, and the quartz crystal sensor 134 outputs data to the data processing system 12 according to the change of the chip 135.

[0036] After the data processing system 12 processes data, the data is displayed on the LED display screen 129.

[0037] During the process that the sample liquid microfluidic system 16 flows to the quartz crystal microbalance system 13, the control system 11 adjusts the rotating speed of the motor 112 to control the delivery amount of the fluid, and the sensor 113 and the control system monitor and optimize the working state of the peristaltic pump in real time.

[0038] The system circuit 119 is maintained through the back plate 213, and the microfluidic system 16 is maintained through the side plate 214.

[0039] The working principle of the utility model is as follows:

[0040] A kind of digital quartz crystal microbalance, including integrated system 1 and main part 2.The integrated system 1 contains constant temperature system 15, microfluidic system 16, quartz crystal microbalance system 13, control system 11, data processing system 12, realize the automation control of each subsystem of instrument by using embedded system, and each subsystem integrated control is completed in combination with system programming.Main part design is around the layout of microfluidic channel of microfluidic chip and quartz crystal sensor 134, connect QCM sensor with the microfluidic channel of microfluidic chip, ensure that fluid can be contacted with sensor through channel, accurately control fluid flow by peristaltic pump 161 and microfluid channel, there is sample cell and waste liquid pool, for storing sample and collecting waste liquid;Mechanical support design is carried out to instrument support structure, and internal structure protection design is carried out to each control and interface unit.

[0041] Before the scheme detection, constant temperature system 15 needs to be started in advance, target temperature is set, after reaching target temperature, the system will issue a reminder.

[0042] When quartz crystal microbalance chip 135 needs to be cleaned, chip 135 can be directly cleaned through control system 11;If chip 135 needs to be replaced, chip storage can be opened through switch button.

[0043] 8 sample reagents can be placed at the same time, and the sampler is controlled by the control system 11 to take samples, and the peristaltic pump and the 161 microfluidic channel complete sample liquid transmission, and the process can control the order of the sampled reagents.

[0044] When the sample liquid is detected, the temperature setting of the constant temperature system 15 is completed by the control system 11, the sample reagent 151 is manually placed, the sample sampling and introduction are operated by the control system 11, the sample liquid processing of the microfluidic system is operated by the control system 11, the sample separation and waste liquid flowing into the waste liquid pool are completed, the sample liquid to be detected is introduced into the microfluidic system 16 for reaction by the control system 11, the separation and detection are completed, the data analysis is completed by the data processing system, and the detection result is displayed on the LED display screen 129, and after the detection is completed, the waste liquid pool liquid is manually treated.

[0045] The scheme mainly realizes automatic operation, multi-parameter detection and high-precision measurement through integrated design; when using the system, it is necessary to pay attention to regular equipment maintenance and calibration, to ensure that the microfluidic chip and the fluid control system are closely and reliably connected, and to avoid leakage, to ensure that the microfluidic chip and the fluid control system are closely and reliably connected, and to avoid leakage. The human-computer interaction system interface is not shown in the scheme, and the overall system design meets the ergonomic design specification, and in addition, the system software needs to be updated regularly to improve performance and compatibility and ensure system stability.

[0046] Finally, it should be pointed out that: first, in the description of the present application, it should be pointed out that unless otherwise specified and limited, the terms "installation", "connection", "connection" should be understood broadly, which can be mechanical connection or electrical connection, or the communication between two elements, or direct connection, "up", "down", "left", "right" and the like are only used to indicate the relative positional relationship, when the absolute position of the described object changes, the relative positional relationship may change;

[0047] Secondly: the utility model discloses the embodiment of the drawings, only relate to the structure involved in the embodiment of the present disclosure, other structures can refer to the usual design, in the case where there is no conflict, the same embodiment and different embodiments of the utility model can be combined with each other;

[0048] Finally: the preferred embodiments of the utility model are described above, and the utility model is not limited, it should be pointed out that for ordinary skilled persons in the technical field, on the premise of not departing from the technical principles of the present application, a plurality of improvements and modifications can be made, and these improvements and modifications should be regarded as the protection scope of the utility model.

Claims

1. A digital microfluidics system comprising a quartz crystal microbalance sensor, characterized in that: The integrated system (1) and the main body (2) are composed of; The integrated system (1) includes a control system (11), a data processing system (12), a quartz crystal microbalance system (13), a constant temperature system (15) and a microfluidic system (16), the control system (11) and the data processing system (12) are connected through electrical signals, the data processing system (12) and the quartz crystal microbalance system (13), the constant temperature system (15) and the microfluidic system (16) are connected through electrical signals; The main body (2) includes an instrument structure (21), a power module (22) and a pipeline and connector (23), the power module (22), the control system (11), the data processing system (12), the quartz crystal microbalance system (13), the constant temperature system (15) and the microfluidic system (16) are fixed in the instrument structure (21), the power module (22) powers the control system (11), the data processing system (12), the quartz crystal microbalance system (13), the constant temperature system (15) and the microfluidic system (16), the pipeline and connector (23) are used to connect the power module (22) and the control system (11), the data processing system (12), the quartz crystal microbalance system (13), the constant temperature system (15) and the microfluidic system (16).

2. A digital microfluidics system comprising a quartz crystal microbalance sensor according to claim 1, characterized in that: The quartz crystal microbalance system (13) is installed with a chip storage (132) which can be ejected, a chip (135) is placed in the chip storage (132), the chip (135) is linked with a quartz crystal sensor (134) after the chip storage (132) is reset, a press storage switch (131) is installed on the instrument structure (21) to control the ejection of the chip storage (132), a handle (133) is installed on the outer end surface of the chip storage (132).

3. The digital microfluidics system comprising a quartz crystal microbalance sensor of claim 1, wherein: The constant temperature system (15) includes a bracket for placing sample reagents (151) and waste liquid bottles (91), a thermostat (152) in the instrument structure (21) provides temperature.

4. The digital microfluidics system comprising a quartz crystal microbalance sensor of claim 1, wherein: The microfluidic system (16) controls a peristaltic pump (161) and a sampling machine (111) to sample and deliver reagents to a microfluidic chip (162), a detection and analysis subsystem (121) in the data processing system (12) detects, collects data and analyzes the reagent reaction on the microfluidic chip (162).

5. The digital microfluidics system comprising a quartz crystal microbalance sensor of claim 1, wherein: The quartz crystal microbalance system (13) sends instructions through the control system (11), so that the microfluidic system (16) delivers reagents into the chip storage (132) through a microfluidic pipeline (139), the quartz crystal sensor (134) records the changes of the chip (135) and outputs the recorded data to the data processing system (12).

6. The digital microfluidics system comprising a quartz crystal microbalance sensor of claim 1, wherein: An LED display screen (129) for data display is installed on the instrument structure (21).

7. A digital microfluidics system comprising a quartz crystal microbalance sensor according to claim 2, wherein: The instrument structure (21) further includes a back plate (213) and a side plate (214).

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