Detection swab filled with aniline blue staining solution

Abstract

The utility model relates to a detection swab filled with aniline blue staining fluid, which effectively solves the problems of multiple detection preparation procedures and low detection speed. Comprising a bulb, the bottom of the bulb is open, a plug is connected to the bottom of the bulb in a sealed mode, aniline blue staining fluid is contained in the bulb, a sealed glass tube is further placed in the bulb, a hydrogen peroxide solution is contained in the glass tube, a partition tube extends out of the upper end of the plug, a swab is detachably connected to the lower portion of the plug, and the partition tube and the swab are connected in the plug. After the partition tube is broken, the aniline blue staining fluid can enter the swab through the plug, and a test tube is in threaded connection below the plug; the device is simple and ingenious in structure, hydrogen peroxide and aniline blue solution are mixed after sampling, the mixed solution enters the end part of the swab to react with the sample, the sample is dyed, the detection result is judged according to the color, the detection is quick, and the use is convenient.

Description

Technical Field

[0001] This utility model relates to the field of detection technology, and in particular to a detection swab containing aniline blue staining solution. Background Technology

[0002] Aniline blue is a quinone-containing luminescent group produced by a redox reaction between oxygen and hydrogen donors. Due to cellular atypia, the intracellular heme protein (HP protein) undergoes a conformational change, exposing the protoheme (a / b / c / l / m) in its hydrophobic barrel to the hydrophilic ends of the polypeptide chain. Aniline blue has a strong affinity for the iron ions in protoheme under acidic hydrophilic conditions, thus staining it. The degree of protoheme staining is positively correlated with cellular atypia. Detecting cellular atypia through aniline blue staining is an important method for analyzing pathological changes in tissue cells.

[0003] In actual testing, hydrogen peroxide is used as a generator of an acidic hydrophilic environment. It is mixed with aniline blue and reacts with the sample to observe the color change.

[0004] Before use, aniline blue and hydrogen peroxide solution cannot be mixed and need to be separated. In the existing technology, the two solutions are stored separately and then mixed during the test. Moreover, the two solutions are placed in different containers, which requires them to be retrieved separately when used, which is not convenient and quick. Utility Model Content

[0005] To overcome the shortcomings of existing technologies, this invention provides a test swab containing aniline blue staining solution, which effectively solves the problems of numerous test preparation procedures and slow test speed.

[0006] The technical solution to the problem is as follows: a test swab containing aniline blue staining solution, comprising a bulb with an open bottom and a plug sealed to the bottom. The bulb contains aniline blue staining solution, and a sealed glass tube containing hydrogen peroxide solution is also placed inside the bulb. A septum tube extends from the upper end of the plug, and a swab is detachably connected to the lower end of the plug. The septum tube and the swab are connected inside the plug. After the septum tube breaks, the aniline blue staining solution can enter the swab through the plug. A test tube is threadedly connected to the lower end of the plug.

[0007] Preferably, the bulb is made of a light-shielding material, and the bulb includes an inner layer and an outer layer, the inner layer being made of EVA material and the outer layer being made of PVC material.

[0008] Preferably, the partition tube is hollow inside, made of ABS plastic, and has a pre-set indentation on the outer circumference, which allows the partition tube to be quickly broken through the indentation.

[0009] Preferably, the swab is made of rayon and has a sampling head at the end.

[0010] Preferably, the test tube is made of PE.

[0011] Preferably, the glass tube is a low-boron silica glass tube.

[0012] The utility model discloses simple and ingenious structure mixes hydrogen peroxide and aniline blue solution after sampling, and the mixed solution enters the end of swab and sampling and reacts, and the sample is dyed, and the detection result is judged according to the color, and the detection is quick, and convenient to use. BRIEF DESCRIPTION OF DRAWINGS

[0013] Fig. 1 It is the structure schematic diagram of the utility model.

[0014] Fig. 2 It is the cross section schematic diagram of the utility model.

[0015] Fig. 3 It is the explosion structure schematic diagram of the utility model. DETAILED DESCRIPTION

[0016] The specific implementation of the utility model is further explained in detail below in combination with the drawings.

[0017] By Figs. 1 to 3 It can be known that a detection swab 5 with aniline blue dye solution, characterized in that, comprising bulb 1, the bottom of bulb 1 is open, bulb 1 bottom sealingly connected with plug 2, bulb 1 is filled with aniline blue dye solution, bulb 1 still places sealed glass tube 3 in, glass tube 3 is filled with hydrogen peroxide solution, plug 2 upper end has the cut-off pipe 4 that stretches out, plug 2 below detachably connected with swab 5, cut-off pipe 4 and swab 5 are connected in plug 2 inside, after cut-off pipe 4 breaks, aniline blue dye solution can pass through plug 2 and enter swab 5, plug 2 below screw thread connection has test tube 6.

[0018] The utility model discloses in specific use,

[0019] First sampling is carried out, test tube 6 is unscrewed, and sampling is carried out using the sampling head 7 at the end of swab 5, after sampling is completed, test tube 6 is screwed with plug 2, then cut-off pipe 4 and glass tube 3 are broken, so that aniline blue dye solution and hydrogen peroxide solution are mixed, the mixed solution enters swab 5 through the pipeline on plug 2, reaches sampling head 7 along swab 5, and the sample is dyed, and then the detection result is judged according to the color.

[0020] The ball bubble 1 is made of light-proof material, can shield light for the solution, avoids reaction without use to cause invalidation, the ball bubble 1 includes inner layer and outer layer, the inner layer is made of EVA material, the outer layer is made of PVC material, soft texture, convenient for breaking off the partition pipe 4 and glass tube 3, the glass tube 3 uses low borosilicate glass tube, when the glass tube 3 breaks, the glass tube 3 will not produce broken glass slag and sharp, avoids pricking the outer wall of the ball bubble 1, there is no security risk.

[0021] The partition pipe 4 is empty inside, the partition pipe 4 is made of ABS plastic material, the outer side of the partition pipe 4 is provided with a preset indentation, the partition pipe 4 can be quickly broken off through the indentation, after the partition pipe 4 breaks, the aniline blue dyeing solution and the hydrogen peroxide mixed solution can enter the swab 5 through the cavity inside the partition pipe 4 and reach the end of the swab 5, and the sampling is detected.

[0022] The swab 5 is made of artificial silk material, the swab 5 is provided with a sampling head 7 at the end, the artificial silk material has strong liquid absorption, is soft and does not contain PCR inhibiting substances, and liquid detection is facilitated.

[0023] The test tube 6 is made of PE material, is transparent itself and is convenient for observing color change.

[0024] When the sample and the solution are mixed for minutes, the color change of the sampling head 7 is observed:

[0025] . No color change: negative, no atypia change is found in cells;

[0026] . Light blue: suspicious positive, cells may have atypia change;

[0027] . Blue-green, blue: positive, cells have atypia change;

[0028] . Dark blue: strong positive, cells have obvious atypia change.

[0029] If the reaction solution is immediately deep blue and then turns into dark yellow or brown red, it indicates that blood is mixed in the sample, and it should also be regarded as strong positive.

[0030] If the reaction solution slowly appears pink or yellow, it indicates that more mucus is mixed in the sample, and there is still a possibility of showing blue, and the observation time should be extended for one minute.

[0031] Compared with the prior art, the utility model has the following beneficial effects: two kinds of solutions are isolated and placed in the ball bubble, are mixed when being broken off the protective container, and are mixed with the sample, the sample is dyed, the detection result is judged according to the color change, it is fast and convenient, greatly simplifies the detection process, improves the detection speed, and optimizes the detection result.

Claims

1. A test swab equipped with an aniline blue staining solution, characterized by, The utility model relates to a kind of aniline blue dyeing liquid sampling device, including bulb (1), the bottom of bulb (1) is open, bulb (1) bottom sealingly connected with plug (2), bulb (1) inside contains aniline blue dyeing liquid, bulb (1) inside is also placed with sealed glass tube (3), glass tube (3) contains hydrogen peroxide solution, plug (2) upper end extends with partition pipe (4), plug (2) below detachably connected with swab (5), partition pipe (4) and swab (5) are connected in plug (2) inside, after partition pipe (4) breaks, aniline blue dyeing liquid can pass through plug (2) and enter swab (5), plug (2) below screw thread connection has test tube (6).

2. The test swab with aniline blue staining solution according to claim 1, characterized in that, The bulb (1) is made of light-shielding material, and the bulb (1) comprises an inner layer and an outer layer.

3. The test swab with aniline blue staining solution according to claim 1, characterized in that, The partition pipe (4) is hollow, and the partition pipe (4) is made of ABS plastic material.

4. The test swab with aniline blue staining solution according to claim 1, characterized in that, The swab (5) is made of artificial silk material, and the swab (5) is provided with a sampling head (7) at the end.

5. The test swab with aniline blue staining solution according to claim 1, characterized in that, The test tube (6) is made of PE material.

6. The test swab with aniline blue staining solution according to claim 1, characterized in that, The glass tube (3) is a low-boron-silicon glass tube.

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