Sample processing device for microbial gene sequencing

Abstract

The utility model relates to the technical field of gene sequencing, and discloses a sample processing device for microbial gene sequencing, which comprises a console, the top of the console is fixedly connected with a fixing frame, the top of the fixing frame is provided with a shaking mechanism, and the top of the shaking mechanism is provided with a placing mechanism; and the shaking mechanism comprises a rotating disc and a motor, the motor is arranged in the control table, the bottom of the rotating disc is fixedly connected with the output end of the motor, the top of the rotating disc is fixedly connected with a protruding block, a sliding plate is arranged at the top of the rotating disc, and a fixing frame is fixedly connected to the bottom of the sliding plate. According to the utility model, the motor drives the turntable to rotate, so that the bump pushes the fixed frame to move, the fixed frame is connected to the bottom of the sliding plate and limited by the guide rod, and the sliding plate only shakes left and right, so that the top test tube swings along with the fixed frame, thereby realizing the effect of uniformly mixing samples, and ensuring that the samples in the test tubes are uniformly mixed through the matching of the structures; and the stability of experimental data is improved.

Description

Technical Field

[0001] This utility model relates to the field of gene sequencing technology, and in particular to a sample processing device for microbial gene sequencing. Background Technology

[0002] Gene sequencing technology is widely used in microbiology research, including pathogen identification, microbial community analysis, and detection of drug resistance genes. Before gene sequencing, microbial samples typically undergo a series of pretreatment procedures, such as culturing, lysis, DNA extraction, and purification, to ensure the accuracy and stability of subsequent sequencing data. Sample processing equipment is a key component of the gene sequencing workflow, enabling the processing of microbial samples under standardized conditions, improving experimental efficiency, and reducing human error.

[0003] Existing microbial gene sequencing sample processing devices typically include functional modules such as centrifugation, filtration, lysis, and extraction. For example, some devices use mechanical vibration or ultrasound to treat microbial cells to accelerate cell lysis and improve DNA release efficiency; others concentrate samples through membrane filtration or centrifugation to remove impurities and improve the purity of the target DNA. Furthermore, some devices integrate automated pipetting systems, reducing manual operation and improving sample throughput and consistency. These traditional sample pretreatment methods can improve experimental efficiency and sample processing quality to a certain extent, meeting the basic requirements of gene sequencing. However, during the period after sample processing, when the samples are placed in test tubes awaiting sequencing, they are in a static state and may separate into layers. Currently, researchers usually mix these layers by manually shaking the test tubes, which increases the number of steps and prevents the processing of samples from the same batch, thus affecting work efficiency. Therefore, a sample processing device for microbial gene sequencing is proposed to solve the above problems. Utility Model Content

[0004] To overcome the above shortcomings, this utility model provides a sample processing device for microbial gene sequencing, which aims to improve the problem of the existing technology that uses manual shaking of test tubes for mixing, which increases the number of operation steps.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] A sample processing device for microbial gene sequencing includes a control panel, a fixed frame fixedly connected to the top of the control panel, a shaking mechanism on the top of the fixed frame, and a placement mechanism on the top of the shaking mechanism.

[0007] The shaking mechanism includes a turntable and a motor. The motor is located inside the control panel. The bottom of the turntable is fixedly connected to the output end of the motor. A protrusion is fixedly connected to the top of the turntable. A sliding plate is provided on the top of the turntable. A fixed frame is fixedly connected to the bottom of the sliding plate. The protrusion is located inside the fixed frame. A guide rod is fixedly connected to the bottom of the sliding plate. A fixed block is fixedly connected to the top of the fixed frame. The side wall of the guide rod is slidably connected inside the fixed block.

[0008] As a further description of the above technical solution:

[0009] The placement mechanism includes a placement frame and a limiting frame. The placement frame is disposed on the top of the sliding plate, and the limiting frame is fixedly connected to the top of the placement frame.

[0010] As a further description of the above technical solution:

[0011] The placement rack has a sponge pad inside, and the groove inside the sponge pad corresponds to the groove inside the limiting frame.

[0012] As a further description of the above technical solution:

[0013] The limiting frame has multiple rubber rings fixedly connected inside, and the rubber rings are all set in the groove.

[0014] As a further description of the above technical solution:

[0015] The top of the limiting frame is provided with horizontal and vertical numbering lines.

[0016] As a further description of the above technical solution:

[0017] The side wall of the placement rack is fixedly connected to a side plate, and a fixing bolt is threaded inside the side plate. The bottom of the fixing bolt is threaded inside the sliding plate.

[0018] As a further description of the above technical solution:

[0019] Each guide rod has a limit plate fixedly connected to one end.

[0020] As a further description of the above technical solution:

[0021] The control panel is equipped with a switch, control knob and display screen, all of which are electrically connected to the control panel. The switch is electrically connected to the motor.

[0022] This utility model has the following beneficial effects:

[0023] 1. In this utility model, the motor drives the turntable to rotate, which in turn causes the protrusion to move in a circular motion, further pushing the fixed frame to move. Since the fixed frame is connected to the bottom of the sliding plate and is restricted by the guide rod, the sliding plate only wobbles left and right, causing the top test tube to swing accordingly, thereby achieving the effect of sample mixing. Through the cooperation between the above structures, the sample in the test tube is ensured to be mixed evenly, improving the stability of experimental data.

[0024] 2. In this utility model, the rubber ring inside the limiting frame increases the friction and ensures the stability of the test tube placement. The sponge pad inside the placement rack protects the bottom of the test tube and prevents damage. In addition, the modular test tube rack and sliding plate are designed separately for easy carrying. Attached Figure Description

[0025] Figure 1 This is a three-dimensional schematic diagram of a sample processing device for microbial gene sequencing proposed in this utility model;

[0026] Figure 2 This is a schematic diagram of the shaking mechanism of a sample processing device for microbial gene sequencing proposed in this utility model.

[0027] Figure 3 This is a cross-sectional view of the mounting frame of a sample processing device for microbial gene sequencing proposed in this utility model;

[0028] Figure 4 This is a schematic diagram of the placement mechanism of a sample processing device for microbial gene sequencing proposed in this utility model.

[0029] Legend:

[0030] 1. Control panel; 2. Fixing frame; 3. Turntable; 4. Motor; 5. Protrusion; 6. Sliding plate; 7. Fixing frame; 8. Fixing block; 9. Guide rod; 10. Limiting plate; 11. Placement rack; 12. Side plate; 13. Fixing bolt; 14. Limiting frame; 15. Sponge pad; 16. Rubber ring; 17. Numbering line; 18. Switch; 19. Control button; 20. Display screen. Detailed Implementation

[0031] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0032] Reference Figures 1-3This utility model provides an embodiment of a sample processing device for microbial gene sequencing, comprising a control panel 1, a fixed frame 2 fixedly connected to the top of the control panel 1, a shaking mechanism on the top of the fixed frame 2, which can shake the sample inside the test tube to even out the sample, improving the accuracy of subsequent gene sequencing; a placement mechanism on the top of the shaking mechanism ensures the stability of the test tube placement; the shaking mechanism includes a turntable 3 and a motor 4, the motor 4 is located inside the control panel 1, the bottom of the turntable 3 is fixedly connected to the output end of the motor 4, when the motor 4 is started, it drives the turntable 3 to rotate; a protrusion 5 is fixedly connected to the top of the turntable 3, the protrusion 5 is located on the outside of the turntable 3, and the protrusion 5 performs circular motion as the turntable 3 rotates; a sliding plate 6 is provided on the top of the turntable 3, a fixed frame 7 is fixedly connected to the bottom of the sliding plate 6, and the protrusion 5 is located inside the fixed frame 7. In the part, while the protrusion 5 is performing circular motion, it can further drive the sliding plate 6 to move through the fixed frame 7. The bottom of the sliding plate 6 is fixedly connected to the guide rod 9, and the top of the fixed frame 2 is fixedly connected to the fixed block 8. The side wall of the guide rod 9 is slidably connected to the inside of the fixed block 8, which has the effect of restricting the movement direction of the sliding plate 6, so that the sliding plate 6 can only move along the sliding direction of the guide rod 9. One end of the guide rod 9 is fixedly connected to the limit plate 10. The control panel 1 is equipped with a switch 18, a control button 19 and a display screen 20, all of which are electrically connected to the control panel 1. The switch 18 is electrically connected to the motor 4, and the start and stop of the motor 4 can be controlled by the switch 18. The control button 19 is used to adjust the running time of the motor 4, and the display screen 20 is used to display the remaining working time of the motor 4. The control panel 1 is a known technology and can achieve the effect of controlling the running time of the motor 4.

[0033] Reference Figure 4 The placement mechanism includes a placement rack 11 and a limiting rack 14. The placement rack 11 is located on top of the sliding plate 6, and the limiting rack 14 is fixedly connected to the top of the placement rack 11. The limiting rack 14 and the placement rack 11 are joined together to form a complete test tube rack. A sponge pad 15 is provided inside the placement rack 11 to protect the bottom of the test tubes and prevent them from being bumped. The groove inside the sponge pad 15 corresponds to the groove inside the limiting rack 14 to ensure smooth insertion of the test tubes. Multiple rubber rings 16 are fixedly connected inside the limiting rack 14. Each rubber ring 16... Set in the groove, the rubber ring 16 always fits against the outside of the test tube when it is inserted, increasing friction and ensuring the stability of the test tube after placement. The top of the limiting frame 14 is provided with horizontal and vertical numbering lines 17, which can be used by the staff to quickly find the corresponding test tube. The side plate 12 is fixedly connected to the side wall of the placement rack 11, and the fixing bolt 13 is threaded inside the side plate 12. The bottom of the fixing bolt 13 is threaded inside the sliding plate 6. The test tube rack and the sliding plate 6 are designed separately, which makes it more convenient to move the equipment.

[0034] Working principle: When using this device to shake the sample storage reagent tubes for gene sequencing, first insert the reagent tube downwards from the inside of the rubber ring 16, so that the lower end of the test tube is inserted into the inside of the sponge pad 15. At the same time, the rubber ring 16 will be tightly attached to the outer wall of the test tube to ensure its stability. After placement, adjust the device usage time through the control button 19. After the time is adjusted, press the switch 18 to start the motor 4, which drives the turntable 3 to rotate, thereby driving the protrusion 5 to perform a circular motion. When the protrusion 5 moves, it will further drive the fixed frame 7 to move. Since the fixed frame 7 is connected to the bottom of the sliding plate 6, and the sliding plate 6 is restricted by the guide rod 9, the fixed frame 7 cannot follow the protrusion 5 to perform a circular motion, but only achieves the effect of swaying left and right, so that the test tube placed on the top sways left and right to achieve the effect of shaking the sample.

[0035] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A sample processing device for sequencing of microbial genes, comprising a console (1), characterized in that: The top of the console (1) is fixedly connected with a fixed frame (2), the top of the fixed frame (2) is provided with a shaking mechanism, and the top of the shaking mechanism is provided with a placing mechanism. The shaking mechanism comprises a rotating disc (3) and a motor (4), the motor (4) is arranged in the console (1), the bottom of the rotating disc (3) is fixedly connected with the output end of the motor (4), the top of the rotating disc (3) is fixedly connected with a protruding block (5), the top of the rotating disc (3) is provided with a sliding plate (6), the bottom of the sliding plate (6) is fixedly connected with a fixed frame (7), the protruding block (5) is arranged in the fixed frame (7), the bottom of the sliding plate (6) is fixedly connected with a guide rod (9), the top of the fixed frame (2) is fixedly connected with a fixed block (8), and the sidewall of the guide rod (9) is slidably connected in the fixed block (8).

2. The sample processing device for microbial gene sequencing of claim 1, wherein: The placing mechanism comprises a placing frame (11) and a limiting frame (14), the placing frame (11) is arranged at the top of the sliding plate (6), and the limiting frame (14) is fixedly connected at the top of the placing frame (11).

3. A sample processing device for sequencing microorganisms according to claim 2, wherein: The inside of the placing frame (11) is provided with a sponge pad (15), and the inside groove of the sponge pad (15) corresponds to the inside groove of the limiting frame (14).

4. The sample processing device for microbial gene sequencing of claim 3, wherein: The inside of the limiting frame (14) is fixedly connected with a plurality of rubber rings (16), and the rubber rings (16) are arranged in the grooves.

5. The sample processing device for microbial gene sequencing of claim 3, wherein: The top of the limiting frame (14) is provided with transverse and longitudinal numbering lines (17).

6. The sample processing device for microbial gene sequencing of claim 2, wherein: The sidewall of the placing frame (11) is fixedly connected with a side plate (12), the inside of the side plate (12) is threadedly connected with a fixing bolt (13), and the bottom of the fixing bolt (13) is threadedly connected in the inside of the sliding plate (6).

7. The sample processing device for microbial gene sequencing of claim 1, wherein: One end of the guide rod (9) is fixedly connected with a limiting plate (10).

8. The sample processing device for microbial gene sequencing of claim 1, wherein: The inside of the console (1) is provided with a switch (18), a control knob (19) and a display screen (20), which are electrically connected with the console (1), and the switch (18) is electrically connected with the motor (4).

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