Explosion-proof device for cryopreservation tube

By designing an explosion-proof device for cryopreservation tubes, and utilizing pressure relief valves and buffer devices to release the pressure of liquid nitrogen gas, the problem of cryopreservation tubes bursting in a liquid nitrogen environment was solved, ensuring the safety and survival rate of cell fluid.

CN224234558UActive Publication Date: 2026-05-15JILIN NEW SAIER BIOTECHNOLOGY CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
JILIN NEW SAIER BIOTECHNOLOGY CO LTD
Filing Date
2025-05-28
Publication Date
2026-05-15

AI Technical Summary

Technical Problem

When traditional cryovials are used in a liquid nitrogen environment, they cannot effectively depressurize, causing the gas to expand rapidly and the cryovial to burst, increasing safety hazards and damaging the cell fluid.

Method used

An explosion-proof device for cryogenic tubes was designed, comprising a buffer device consisting of a tube body, a tube cap, an inner tube, a pressure relief valve, a moving ring, a guide rod, and a spring. The pressure relief valve releases gas, buffering nitrogen gas pressure changes to prevent the cryogenic tube from bursting.

Benefits of technology

It effectively prevents cryopreservation tubes from bursting, protects the integrity of the cell solution, reduces safety hazards, and ensures the survival rate of the cell solution.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model discloses a cryopreservation tube explosion-proof device, which relates to the technical field of cryopreservation tube explosion-proof appliances and comprises a tube body, internal threads are arranged on the inner wall of the upper end of the tube body, pins are arranged on a shell of the lower end of the tube body, a tube cover is arranged at the upper end of the tube body, a threaded groove is arranged on the outer side of the lower end of the tube cover, and the tube cover is in threaded connection with the tube body. According to the anti-freezing pipe, the movable ring, the guide rod, the spring and the inner pipe form a buffer device conveniently, the pressure at the upper end of the pipe body is gradually increased while liquid nitrogen is vaporized, the movable ring is extruded to move downwards to extrude the spring for compression, the moving space is increased for the upper end of the pipe body, the time is prolonged for gas exhaust, and the anti-freezing pipe is not prone to exploding; gas in the inner pipe can be conveniently discharged through the pressure release valve, the pressure in the inner pipe is reduced, the completeness of the cell sap is ensured, and the problems that the anti-freezing pipe is exploded, the cell sap is damaged and the like are finally solved.
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Description

Technical Field

[0001] This utility model relates to the technical field of explosion-proof equipment for cryopreservation tubes, and in particular to an explosion-proof device for cryopreservation tubes. Background Technology

[0002] Traditional cryoprotective tubes require storage in liquid nitrogen tanks after treatment to allow cell sap to enter a dormant state at low temperatures, ensuring cell survival. However, a small amount of liquid nitrogen can seep into the tube body and cap through the threaded gaps. Liquid nitrogen has a very low boiling point, and when its temperature rises to the boiling point, it produces a large amount of gas. Conventional cryoprotective tubes cannot effectively release the pressure in time to expel the gas, causing rapid gas expansion and potentially leading to the tube bursting. This increases safety hazards for researchers and damages the cell sap, rendering it unusable for experiments. Therefore, solutions to these technical problems are needed. Utility Model Content

[0003] The purpose of this invention is to address the shortcomings of existing technologies by proposing an explosion-proof device for cryopreservation tubes.

[0004] To achieve the above objectives, the present invention adopts the following technical solution: an explosion-proof device for cryopreservation tubes, comprising a tube body, wherein the inner wall of the upper end of the tube body is provided with an internal thread, the outer shell of the lower end is provided with a pin, and a tube cap is provided at the upper end of the tube body, wherein a threaded groove is provided on the outer side of the lower end of the tube cap, and the tube cap is threadedly connected to the tube body.

[0005] Preferably, a spline groove is vertically formed in the middle of the top surface of the tube cap and extends through the cap body, and a first sealing ring is provided at the upper end of the tube body, which is sleeved with the lower end of the tube cap.

[0006] Preferably, an inner tube is vertically installed in the middle of the inner tube body. The upper outer wall of the inner tube is threaded, the middle part is vertically splined, and the lower end has a trumpet-shaped structure. Two mounting grooves are opened at the lower outer end of the inner tube. A second sealing ring and a third sealing ring are respectively fitted into the two mounting grooves. The second sealing ring and the third sealing ring abut against the inner wall of the inner tube body.

[0007] Preferably, the spline in the middle of the inner tube is sleeved with the spline groove in the middle of the top surface of the tube cover, and a pressure relief valve is installed at the upper end of the inner tube, the pressure relief valve being threadedly connected to the inner tube.

[0008] Preferably, the tube body is provided with a movable ring inside, the outer side of the movable ring abuts against the inner wall of the tube body, and the movable ring is sleeved on the lower end of the inner tube. The movable ring has multiple guide holes axially opened in the middle and is sleeved on the inner tube. Multiple guide rods are axially installed at the lower end of the movable ring, and one end of the guide rod passes through the movable ring.

[0009] Preferably, the movable ring has multiple equidistant guide holes on its circumference, and a guide rod is inserted through the guide holes. The lower end of the guide rod is fixed to the top surface of the lower end of the inner tube, and a spring is sleeved on the outside of the guide rod. The spring is placed between the movable ring and the top surface of the lower end of the inner tube.

[0010] Compared with the prior art, the beneficial effects of this utility model are as follows: In this utility model, the moving ring, guide rod, spring and inner tube can be easily assembled into a buffer device, which allows the pressure at the upper end of the tube to gradually increase while the liquid nitrogen is vaporizing. The moving ring is squeezed downward to compress the spring, increasing the activity space at the upper end of the tube and increasing the time for gas to be discharged, making the antifreeze tube less likely to burst. The pressure relief valve facilitates the discharge of gas from the inner tube, reducing the pressure inside the inner tube and ensuring the integrity of the cell fluid. Ultimately, this solves the problems of the antifreeze tube bursting and the cell fluid being damaged. Attached Figure Description

[0011] The accompanying drawings, which are included to provide a further understanding of the present invention and form part of this application, illustrate exemplary embodiments of the present invention and, together with the description thereof, serve to explain the present invention and do not constitute an undue limitation thereof. In the drawings:

[0012] Figure 1 This is a three-dimensional schematic diagram of the overall structure proposed in this utility model;

[0013] Figure 2 This is a first cross-sectional view of the overall structure proposed in this utility model;

[0014] Figure 3 This is a second cross-sectional view of the overall structure proposed in this utility model;

[0015] Figure 4 This is a three-dimensional schematic diagram of the pipe cap and the first sealing ring structure proposed in this utility model;

[0016] Figure 5 This is a cross-sectional schematic diagram of the pipe cap and the first sealing ring proposed in this utility model.

[0017] The numbers in the diagram are: 1. Pipe body; 2. Pipe cap; 3. Pressure relief valve; 4. First sealing ring; 5. Second sealing ring; 6. Third sealing ring; 7. Spring; 8. Guide rod; 9. Moving ring; 10. Inner pipe. Detailed Implementation

[0018] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0019] Example: See Figure 1-5This utility model discloses an explosion-proof device for cryopreservation tubes, comprising a tube body 1, with an internal thread on the inner wall of the upper end of the tube body 1 and pins on the outer shell of the lower end. A tube cap 2 is provided at the upper end of the tube body 1, and a threaded groove is provided on the outer side of the lower end of the tube cap 2, which is threadedly connected to the tube body 1. A spline groove is vertically formed in the center of the top surface of the tube cap 2 and extends through the cap body. A first sealing ring 4 is provided at the upper end of the tube body 1, and the first sealing ring 4 is sleeved on the lower end of the tube cap 2. An inner tube 10 is vertically installed in the center of the inner side of the tube body 1. The inner tube 10 has threads on its upper outer wall, a spline vertically formed in the middle, and a trumpet-shaped structure at its lower end. Two mounting grooves are formed on the lower outer side of the inner tube 10, with a second sealing ring 5 and a third sealing ring 6 respectively fitted into each groove. The second sealing ring 5 and the third sealing ring 6 abut against the inner wall of the tube body 1. The spline in the middle of the inner tube 10 fits into the spline groove in the middle of the top surface of the tube cap 2. A pressure relief valve 3 is installed at the upper end of the inner tube 10, and the pressure relief valve 3 is threadedly connected to the inner tube 10. The inner tube 1 is equipped with a movable ring 9. The outer side of the movable ring 9 abuts against the inner wall of the inner tube 1 and is sleeved on the lower end of the inner tube 10. The middle of the movable ring 9 has multiple guide holes axially opened and is sleeved on the inner tube 10. Multiple guide rods 8 are axially installed on the lower end of the movable ring 9, and one end of the guide rod 8 passes through the movable ring 9. Multiple equidistant guide holes are opened on the periphery of the movable ring 9. The guide rods 8 pass through the guide holes and are fixed to the top surface of the lower end of the inner tube 10. A spring 7 is sleeved on the outer side of the guide rod 8 and is placed between the movable ring 9 and the top surface of the lower end of the inner tube 10. The spline groove opened on the top surface of the tube cover 2 facilitates the up and down movement of the inner tube 10 and restricts the rotation of the inner tube 10. The second sealing ring 5 and the third sealing ring 6 help prevent liquid nitrogen from entering the bottom of the inner tube 1. The movable ring 9, guide rods 8 and spring 7 cooperate to form a compression device. The pressure relief valve 3 helps to release the pressure inside the inner tube 10. The first sealing ring 4 helps to keep most of the liquid nitrogen out, while the funnel-shaped structure at the lower end of the inner tube 10 helps to isolate the infiltrated liquid nitrogen and cell fluid.

[0020] Working Principle: In the use of this invention, the collected cell fluid is first injected into the tube body 1, and the tube cap 2 is tightened. The cell fluid is then placed in a liquid nitrogen tank for cryogenic preservation, allowing the cell fluid to enter a dormant state. Since the antifreeze tube is in the liquid nitrogen tank for a long time, a small amount of liquid nitrogen will enter the tube body 1 through the thread gap and spline connection gap between the tube body 1 and the tube cap 2. When needed, the antifreeze tube needs to be removed and stored in the refrigerator to increase the internal temperature of the antifreeze tube, so that the cells are gradually awakened and facilitated for subsequent use. During the warming process of the antifreeze tube, the liquid nitrogen gradually vaporizes, causing the internal pressure of the tube body 1 to surge. As the gas increases, the moving ring 9 squeezes downward, causing the spring 7 to compress and increase the gas movement space, which increases the buffer time for gas discharge. The gas released by the liquid nitrogen is discharged through the thread gap and spline connection gap, reducing the internal pressure of the tube body 1. When the liquid nitrogen seeps into the cell fluid storage chamber, the liquid nitrogen temperature increases and gradually vaporizes, increasing the internal pressure of the inner tube 10, which is discharged through the pressure relief valve 3 installed at the top of the inner tube 10.

[0021] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. An explosion-proof device for cryopreservation tubes, comprising a tube body (1), characterized in that: The upper inner wall of the tube body (1) is provided with an internal thread, and the lower outer shell is provided with a pin. The upper end of the tube body (1) is provided with a tube cap (2), and the lower outer side of the tube cap (2) is provided with a threaded groove. The tube cap (2) is threadedly connected to the tube body (1). The upper end of the tube body (1) is provided with a first sealing ring (4). The inner tube (10) is vertically installed in the middle of the inner side of the tube body (1). The lower outer side of the inner tube (10) is provided with two mounting grooves. The two mounting grooves are respectively fitted with a second sealing ring (5) and a third sealing ring (6). The tube body (1) is provided with a moving ring (9). The outer side of the moving ring (9) abuts against the inner wall of the tube body (1). The moving ring (9) is fitted on the lower end of the inner tube (10).

2. The explosion-proof device for cryopreservation tubes according to claim 1, characterized in that: The top surface of the pipe cap (2) has a vertical spline groove that extends through the cap body, and the first sealing ring (4) is sleeved with the lower end of the pipe cap (2).

3. The explosion-proof device for cryopreservation tubes according to claim 1, characterized in that: The upper outer wall of the inner tube (10) is threaded, the middle part is vertically splined, and the lower end has a trumpet-shaped structure. The second sealing ring (5) and the third sealing ring (6) respectively abut against the inner wall of the tube body (1).

4. The explosion-proof device for cryopreservation tubes according to claim 3, characterized in that: The spline in the middle of the inner tube (10) is sleeved with the spline groove in the middle of the top surface of the tube cap (2), and a pressure relief valve (3) is installed at the upper end of the inner tube (10), and the pressure relief valve (3) is threadedly connected to the inner tube (10).

5. The explosion-proof device for cryopreservation tubes according to claim 1, characterized in that: The moving ring (9) has multiple guide holes axially opened in the middle and is sleeved with the inner tube (10). Multiple guide rods (8) are axially installed at the lower end of the moving ring (9), and one end of the guide rod (8) passes through the moving ring (9).

6. The explosion-proof device for cryopreservation tubes according to claim 5, characterized in that: The moving ring (9) has multiple equidistant guide holes on its circumference. A guide rod (8) is inserted through the guide hole. The lower end of the guide rod (8) is fixed to the top surface of the lower end of the inner tube (10). A spring (7) is sleeved on the outside of the guide rod (8). The spring (7) is placed between the moving ring (9) and the top surface of the lower end of the inner tube (10).