Intelligent platelet antibody detection device

Abstract

The utility model belongs to the technical field of platelet antibody detection devices, and discloses an intelligent platelet antibody detection device which comprises a rack, a sample carrier, a driving assembly and a detection module, and the rack forms a main body supporting structure of the device; the sample carrying frame is arranged on the rack and comprises a bottom frame piece and a carrying piece; first convex lugs are fixed to the four corners of the bottom frame part, dampers are vertically fixed to the tops of the first convex lugs, buffer springs are connected to the outer surfaces of the dampers in a sleeving mode, mounting parts are fixed to the tops of the dampers, and locking nuts are arranged on the outer surfaces of the tops of the mounting parts in a threaded sleeving mode. According to the utility model, quick separation and fixation of the carrying piece and the underframe piece are realized, and carrying pieces with different specifications can be replaced without disassembling the whole component. In the moving process of the sample carrier, the buffer spring and the damper can effectively absorb vibration generated by transmission, and the liquid sample in the sample test tube is prevented from shaking left and right.

Description

Technical Field

[0001] This utility model belongs to the technical field of platelet antibody detection devices, specifically relating to an intelligent platelet antibody detection device. Background Technology

[0002] Platelet antibody testing is a crucial step in clinical transfusion safety, pregnancy immune monitoring, and transplant rejection assessment. Its core lies in accurately identifying anti-platelet antibodies in a patient's blood to avoid transfusion ineffectiveness or organ rejection caused by antibodies. Current detection technologies mainly rely on enzyme-linked immunosorbent assays (ELISA) and flow cytometry, which require manual operation for sample dispensing, reagent addition, and result interpretation, resulting in low efficiency and high error rates. In recent years, some automated equipment has been introduced into laboratories. However, in these automated devices, the sample holder is often a single, integrated design, directly fixed to the drive mechanism. This necessitates disassembling the entire unit when changing the sample holder, and the sample holder is susceptible to vibrations caused by the motor's start and stop during movement, leading to lateral movement of the liquid sample in the test tube and affecting the detection results. Utility Model Content

[0003] To address the aforementioned technical problems, this utility model provides an intelligent platelet antibody detection device, which aims to solve to some extent the technical problems in the prior art where the sample carrier in automated equipment is mostly an integrated design, with the sample carrier directly fixed to the drive mechanism, which requires disassembling the entire component when replacing the sample carrier, and the sample carrier is easily affected by vibration caused by the start and stop of the motor during movement, resulting in the sample liquid in the test tube swaying from side to side.

[0004] The technical solution of this utility model is: an intelligent platelet antibody detection device, including a frame, a sample carrier, a drive component and a detection module, wherein the frame forms the main support structure of the device;

[0005] The sample carrier is mounted on the frame, and the sample carrier includes a base frame and a loading component;

[0006] The base frame is fixed with a first lug at each of its four corners. A damper is vertically fixed to the top of the first lug. A buffer spring is sleeved on the outer surface of the damper. A mounting piece is fixed to the top of the damper. A locking nut is threaded onto the outer surface of the top of the mounting piece.

[0007] The four corners of the load-bearing component are provided with second lugs, the top of the mounting component passes through the through hole of the second lug, and the bottom of the locking nut abuts against the top of the second lug;

[0008] The drive assembly is fixed to the frame and connected to the base frame of the sample carrier, and is used to drive the object to move.

[0009] The detection module is mounted on the rack and is used to detect platelet antibodies in sample tubes.

[0010] In some embodiments, the carrier is provided with mounting holes for fixing sample tubes.

[0011] In some embodiments, the inner wall of the mounting hole is provided with an elastic buffer layer, which is made of silicone or rubber and is used to adapt to sample tubes of different diameters.

[0012] In some embodiments, the bottom of the buffer spring abuts against the top of the first lug, and the top of the buffer spring abuts against the bottom of the mounting member.

[0013] In some embodiments, the drive assembly includes a servo motor fixed to a frame, a ball screw fixedly connected to the output end of the servo motor, a ball nut sleeved on the ball screw, and the bottom of the base frame fixed to the top of the ball nut.

[0014] In some embodiments, the drive assembly further includes a slide rod fixed to the frame, the slide rod being arranged parallel to the ball screw, a sliding sleeve being slidably fitted onto the outer surface of the slide rod, and the bottom of the base frame being fixed to the top of the sliding sleeve.

[0015] In some embodiments, a positioning sensor is also included, which is mounted on the frame and electrically connected to the detection module for detecting the real-time position of the sample tubes on the carrier.

[0016] One or more technical solutions provided in the embodiments of this application have at least the following technical effects or advantages:

[0017] The system utilizes dampers, buffer springs, mounting components, and locking nuts to achieve rapid separation and fixation of the carrier and the base frame, allowing for the replacement of carriers of different specifications without disassembling the entire assembly. During the movement of the sample carrier, the buffer springs and dampers effectively absorb vibrations generated by the transmission, preventing the liquid samples inside the test tubes from swaying from side to side. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of this application, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 This is a schematic diagram of the structure of this utility model;

[0020] Figure 2This is a schematic diagram of the sample carrier structure of this utility model;

[0021] Figure 3 This is a schematic diagram of the base frame structure of this utility model;

[0022] Figure 4 This is a schematic diagram of the structure of the carrier component of this utility model;

[0023] Figure 5 This is a bottom view of the structure of this utility model.

[0024] In the attached image:

[0025] 100. Rack;

[0026] 200. Sample carrier;

[0027] 210. Base frame component; 211. First lug; 212. Damper; 213. Buffer spring; 214. Mounting component; 215. Locking nut;

[0028] 220. Loading component; 221. Second lug; 222. Mounting hole;

[0029] 230. Sample tubes;

[0030] 300. Drive assembly; 310. Servo motor; 320. Ball screw; 330. Ball nut; 340. Slide bar; 350. Slide sleeve;

[0031] 400. Detection module. Detailed Implementation

[0032] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this application, and not all of the embodiments. Based on the embodiments of this application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of this application.

[0033] This application is described below with reference to the accompanying drawings and specific embodiments:

[0034] This invention provides an intelligent platelet antibody detection device. Through the arrangement of a damper 212, a buffer spring 213, a mounting component 214, and a locking nut 215, it achieves rapid separation and fixation of the sample carrier 220 and the base frame 210, allowing for replacement of different specifications of the sample carrier 220 without disassembling the entire assembly. During the movement of the sample carrier 200, the buffer spring 213 effectively absorbs vibrations generated by the transmission, preventing the liquid sample in the sample tube 230 from shaking or spilling.

[0035] Specifically: Please refer to Figure 1-5An intelligent platelet antibody detection device includes a frame 100, a sample carrier 200, a drive assembly 300, and a detection module 400. The frame 100 constitutes the main support structure of the device.

[0036] The sample carrier 200 is mounted on the frame 100. The sample carrier 200 includes a base frame 210 and a loading component 220. The base frame 210 has a first lug 211 fixed at each of its four corners. A damper 212 is vertically fixed to the top of the first lug 211. A buffer spring 213 is sleeved on the outer surface of the damper 212. A mounting component 214 is fixed to the top of the damper 212. A locking nut 215 is threaded onto the outer surface of the top of the mounting component 214.

[0037] The container 220 has second lugs 221 at its four corners. The top of the mounting piece 214 passes through the through hole of the second lug 221, and the bottom of the locking nut 215 abuts against the top of the second lug 221. The second lugs 221 on the container 220 are fitted onto the mounting piece 214 and fixed by the locking nut 215. The operator can directly replace the container 220 with one that is compatible with test tubes of different sizes without disassembling the drive assembly 300 or adjusting the frame 100.

[0038] The drive assembly 300 is fixed to the frame 100 and connected to the base frame 210 of the sample carrier 200, used to drive the movement of the carrier 220; the detection module 400 is mounted on the frame 100 and used to detect platelet antibodies in the sample tube 230. By controlling the operation of the detection module 400 and the drive assembly 300 through a preset program, fully automated operation of sample positioning and data acquisition is achieved, reducing errors caused by manual intervention.

[0039] The carrier 220 is provided with mounting holes 222 for fixing sample tubes 230. The inner wall of the mounting hole 222 is provided with an elastic buffer layer, which is made of silicone or rubber, to accommodate sample tubes 230 of different diameters. The mounting hole 222 can be used with sample tubes 230 with barcodes or RFID tags. Through the coordinate mapping between the preset hole position and the detection module 400, the automatic association and traceability of sample information can be realized.

[0040] The bottom of the buffer spring 213 abuts against the top of the first lug 211, and the top of the buffer spring 213 abuts against the bottom of the mounting member 214. The buffer spring 213 is constrained between the first lug 211 and the mounting member 214, preventing axial displacement even under high-frequency vibration. During the movement of the carrier 220, the buffer spring 213 absorbs vibrations from the transmission process, ensuring the stability of the sample tube 230 and preventing detection errors caused by the lateral swaying of the liquid sample.

[0041] The drive assembly 300 includes a servo motor 310, which is fixed to the frame 100. A ball screw 320 is fixedly connected to the output end of the servo motor 310, and a ball nut 330 is fitted onto the ball screw 320. The bottom of the base frame 210 is fixed to the top of the ball nut 330. The operation of the servo motor 310 drives the ball screw 320, and in conjunction with the ball nut 330, converts rotary motion into linear motion, thereby moving the base frame 210.

[0042] The drive assembly 300 also includes a slide bar 340, which is fixed to the frame 100. The slide bar 340 is arranged parallel to the ball screw 320, and a sliding sleeve 350 is slidably fitted onto the outer surface of the slide bar 340. The bottom of the base frame 210 is fixed to the top of the sliding sleeve 350. The low-friction sliding engagement between the sliding sleeve 350 and the slide bar 340 further eliminates minor backlash errors in the transmission of the ball screw 320, ensuring that the load 220 moves smoothly along a straight line.

[0043] An intelligent platelet antibody detection device further includes a positioning sensor mounted on a frame 100 and electrically connected to a detection module 400. The positioning sensor detects the real-time position of the sample tube 230 on a carrier 220. By detecting the real-time position of the carrier 220, the positioning sensor feeds back to the control system of a servo motor 310, dynamically correcting movement errors to ensure that the detection module 400 is always aligned with the center of the sample tube 230. When the carrier 220 moves to a preset detection position, the positioning sensor automatically triggers the detection module 400 to start platelet antibody detection.

[0044] The detection module 400 includes an optical sensing unit (laser emitter, photodetector array), a microfluidic chip, a temperature control module, and a data processing unit. The detection module 400 employs a double-antibody sandwich method: Step 1: A fluorescently labeled anti-human immunoglobulin (e.g., anti-IgG / IgM) antibody is added to the sample tube 230, allowing it to specifically bind to antibodies on the platelet surface (e.g., HLA antibodies, HPA antibodies). Step 2: Using flow cytometry principles, the fluorescent label is excited by a laser, and the fluorescence signal intensity is captured by a high-sensitivity photomultiplier tube to quantitatively analyze the platelet surface antibody concentration. The detection module 400 also incorporates a multi-wavelength LED light source (e.g., 488nm blue light, 640nm red light) and a spectrophotometer, enabling simultaneous detection of different fluorescent labels (e.g., FITC, PE, APC), achieving parallel analysis of multiple antibody targets (e.g., simultaneous detection of anti-GPⅡb / Ⅲa antibodies and anti-CD36 antibodies). By combining forward scattered light (FSC) and side scattered light (SSC), platelet morphology and aggregation status can be monitored in real time, distinguishing normal platelets from antibody-mediated abnormal aggregates and reducing the false positive rate.

[0045] An intelligent platelet antibody detection device also includes a network communication module, which is mounted on the rack 100 and electrically connected to the detection module 400. The network communication module is used to upload the detection results or related information to a remote server or to exchange data with other devices.

[0046] It should be noted that the terms “comprising,” “including,” or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0047] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An intelligent platelet antibody detection device, characterized in that, include: The frame (100) constitutes the main support structure of the device; A sample carrier (200) is disposed on the rack (100), and the sample carrier (200) includes: The base frame (210) has a first lug (211) fixed at its four corners. A damper (212) is vertically fixed at the top of the first lug (211). A buffer spring (213) is sleeved on the outer surface of the damper (212). A mounting piece (214) is fixed at the top of the damper (212). A locking nut (215) is threaded on the outer surface of the top of the mounting piece (214). The carrier (220) has a second lug (221) at its four corners. The top of the mounting part (214) passes through the through hole of the second lug (221), and the bottom of the locking nut (215) abuts against the top of the second lug (221).

2. The intelligent platelet antibody detection device as described in claim 1, characterized in that, The carrier (220) is provided with mounting holes (222) for fixing sample tubes (230).

3. The intelligent platelet antibody detection device as described in claim 2, characterized in that, The inner wall of the mounting hole (222) is provided with an elastic buffer layer to accommodate sample tubes (230) of different diameters.

4. The intelligent platelet antibody detection device as described in claim 1, characterized in that, The bottom of the buffer spring (213) abuts against the top of the first lug (211), and the top of the buffer spring (213) abuts against the bottom of the mounting member (214).

5. The intelligent platelet antibody detection device as described in claim 1, characterized in that, It also includes a drive assembly (300), which is fixed to the frame (100) and connected to the base frame (210) of the sample carrier (200) for driving the carrier (220) to move.

6. The intelligent platelet antibody detection device as described in claim 5, characterized in that, The drive assembly (300) includes a servo motor (310), which is fixed on the frame (100). The output end of the servo motor (310) is fixedly connected to a ball screw (320), and a ball nut (330) is sleeved on the ball screw (320). The bottom of the base frame (210) is fixed to the top of the ball nut (330).

7. The intelligent platelet antibody detection device as described in claim 6, characterized in that, The drive assembly (300) also includes a slide rod (340), which is fixed on the frame (100). The slide rod (340) is arranged parallel to the ball screw (320). A sliding sleeve (350) is slidably sleeved on the outer surface of the slide rod (340). The bottom of the base frame (210) is fixed to the top of the sliding sleeve (350).

8. The intelligent platelet antibody detection device as described in claim 1, characterized in that, It also includes a detection module (400), which is mounted on the rack (100) for detecting platelet antibodies in sample tubes (230).

9. The intelligent platelet antibody detection device as described in claim 8, characterized in that, It also includes a positioning sensor, which is mounted on the frame (100) and electrically connected to the detection module (400) for detecting the real-time position of the sample tube (230) on the carrier (220).

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