A gravity chromatography pre-packed column with improved protein a / g binding capacity

Abstract

The utility model provides a gravity chromatography prepacked column of improving protein A / G binding capacity relates to prepacked column technical field, including the column body, flow regulation mechanism sets up at the bottom of column body for the flow rate of regulation and control liquid, flow regulation mechanism includes the shell, shaft hole, pivot and baffle, the utility model discloses in, when using prepacked column, the user can rotate adjusting wheel two, drives threaded rod push rod to draw out from the rotary disc, and the clamping block will be under the push of spring, with the separation of the inner wall of clamping ring, removes the limitation of pivot, at this moment, people only need to rotate the baffle through pivot, can regulate and control the flow rate of internal liquid, the device can ensure that the protein in prepacked column combines fully, improves the recovery efficiency and purity of target protein, also makes prepacked column not to need to replace equipment or filler to can adapt to different experimental conditions, greatly enhanced the operation flexibility.

Description

Technical Field

[0001] This utility model relates to the field of pre-packed column technology, and in particular to a gravity chromatography pre-packed column for improving the A / G binding ability of proteins. Background Technology

[0002] Pre-packed columns are chromatographic separation devices that are pre-filled with chromatographic media and packaged in a standardized manner. They can be used directly for the purification, desalting, or analysis of biomolecules. Their design goal is to simplify the operation process, reduce human error, and provide laboratories with ready-to-use separation tools.

[0003] Most pre-packed columns on the market today have the drawback of non-adjustable flow rates. Setting a fixed flow rate can easily lead to the following problems: some high-viscosity or high-concentration samples may not be separated sufficiently due to improper flow rate matching, while low-concentration or high-molecular-weight samples may have their separation time prolonged due to excessively slow flow rates, thus increasing the experimental cycle. In addition, the design of a single flow rate cannot meet the dual requirements of separation speed and accuracy in different experimental scenarios, which limits the applicability of pre-packed columns in complex sample systems. Utility Model Content

[0004] The purpose of this invention is to solve the problem that the pre-packed column cannot adjust the flow rate during use, which affects the separation effect and has low adaptability. Therefore, this invention proposes a gravity chromatography pre-packed column that improves the binding ability of protein A / G.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: a gravity chromatography pre-packed column for improving the A / G binding ability of proteins, comprising a column body;

[0006] The flow regulation mechanism is located at the bottom of the column and is used to regulate the flow rate of the liquid;

[0007] The flow regulation mechanism includes a housing, a shaft hole, a rotating shaft, and a baffle. The shaft hole is located at the output end of the housing, and the rotating shaft is rotatably connected inside the shaft hole. When the rotating shaft rotates, it drives the baffle to rotate inside the housing. By adjusting the angle of the baffle, the liquid flow rate can be regulated. When the rotating shaft is driven to rotate, it can drive the baffle fitted on the outer wall to rotate inside the housing. As the baffle rotates, its relative position with the housing changes, thereby adjusting the flow area of ​​the liquid discharged from the column. When the flow area increases, the flow rate decreases; when the flow area decreases, the flow rate increases. By adjusting the flow rate, the binding time between the protein and the pre-packed column packing material can be controlled, ensuring that the target protein is fully bound and reducing the low binding efficiency or incomplete elution caused by improper flow rate. This also makes the pre-packed column flexible to adapt to different experimental conditions without the need to change equipment or packing material.

[0008] Preferably, an adjusting wheel is fixedly installed on one side of the outer wall of the rotating shaft, and a clamping ring is fixedly installed on one side of the outer wall of the outer shell. A connecting seat is movably inserted into the inner surface of the clamping ring, and a rotating disk is fixedly installed on one side of the outer wall of the connecting seat. The outer surface of the rotating disk is movably inserted into the inside of the clamping ring. The adjusting wheel allows the user to easily drive the rotating shaft to rotate, and the clamping ring can cooperate with the rotating disk to fix or loosen the rotating shaft, thereby preventing the rotating shaft from rotating due to external force collision or vibration, which would cause changes in flow rate.

[0009] Preferably, the inner surface of the connecting seat is threaded with a threaded push rod, and an adjusting wheel is fixedly installed on one side of the outer wall of the threaded push rod. The interior of the rotating disk has a set of movable slots, and springs are movably inserted into the inner surface of each set of movable slots. The tail end of the threaded push rod is not threaded and is movably inserted into the connecting seat, which facilitates the movement of the threaded push rod and related devices. After the adjusting wheel is rotated, the threaded push rod is driven to rotate and, under the action of the thread, advances inside the connecting seat and extends into the rotating disk.

[0010] Preferably, a fixing ring is fixedly installed on one side of the outer wall of each set of springs, and the outer wall of each set of fixing rings is fixedly inserted into the interior of the movable groove. A locking block is movably inserted into the inner wall of each set of movable grooves, and one side of the outer wall of each set of springs is fixedly connected to one side of the outer wall of the locking block. The outer wall of each set of locking blocks is fixedly inserted into the interior of the fixing rings. When the threaded push rod advances, its first end will contact the arc-shaped surface of the locking block in the movable groove and push the locking block to move outward, pulling the spring and retracting it into the movable groove. At this time, the other end of the locking block will be in close contact with the inner wall of the locking ring, locking the rotating shaft on one side of the rotating disk to prevent it from rotating accidentally, ensuring that the binding time between the internal protein and the pre-filled column packing remains unchanged.

[0011] Preferably, the outer wall of the column is movably fitted with a top cover, the outer wall of the column is fixedly fitted with a fixing sleeve, and the outer wall of the fixing sleeve is movably fitted with a connecting rope. The top cover can prevent liquid splashing, protect the safety of operators, and avoid contamination of protein A / G packing material or samples.

[0012] Preferably, the inner surface of the connecting rope is movably inserted with a connector, and the outer surface of the connector is movably inserted into the interior of the column. The connecting rope connects the top cover to the column, which can effectively prevent the top cover from being lost.

[0013] Preferably, a piston is fixedly installed at the bottom of the top cover, and the outer wall of the piston is movably inserted into the inside of the column. The outer wall of the column is provided with scale lines, and the outer wall of the flow regulating mechanism is movably fitted with a lower plug. The piston can improve the sealing of the liquid inside the top cover, the scale lines allow people to know the volume of the liquid inside in a timely manner, and the lower plug prevents the buffer solution or elution solution from leaking from the bottom when the pre-installed column is not in use.

[0014] Compared with the prior art, the advantages and positive effects of this utility model are as follows:

[0015] 1. In this utility model, when using the pre-packed column, the user can rotate the second adjusting wheel to pull the threaded rod pusher out of the rotating disk. The locking block will then separate from the inner wall of the locking ring under the push of the spring, releasing the restriction on the rotating shaft. At this time, people only need to drive the baffle to rotate by rotating the shaft to adjust the flow rate of the internal liquid. This device can ensure that the protein in the pre-packed column is fully bound, improve the recovery efficiency and purity of the target protein, and also make the pre-packed column adaptable to different experimental conditions without changing equipment or packing materials, greatly enhancing the operational flexibility. Attached Figure Description

[0016] Figure 1 This invention provides a three-dimensional view of the main structure of a gravity chromatography pre-packed column for improving protein A / G binding ability;

[0017] Figure 2 A plan view of a gravity chromatography pre-packed column for improving protein A / G binding ability is proposed in this utility model;

[0018] Figure 3 A cross-sectional perspective view of a gravity chromatography pre-packed column for improving protein A / G binding capacity is provided in this utility model.

[0019] Figure 4 A top-down exploded view of a flow regulation mechanism in a gravity chromatography pre-packed column for improving protein A / G binding capacity, as proposed in this utility model;

[0020] Figure 5 A cross-sectional perspective view of a flow regulation mechanism in a gravity chromatography pre-packed column for improving protein A / G binding capacity is provided in this utility model.

[0021] Figure 6 This invention provides a cross-sectional view of a flow regulation mechanism in a gravity chromatography pre-packed column for improving protein A / G binding capacity.

[0022] Legend:

[0023] 1. Column; 2. Top cover; 3. Fixing sleeve; 4. Connecting rope; 5. Connector; 6. Piston; 7. Scale line; 8. Flow regulating mechanism; 801. Outer shell; 802. Shaft hole; 803. Rotating shaft; 804. Baffle; 805. Adjusting wheel one; 806. Clamping ring; 807. Connecting seat; 808. Rotary disk; 809. Threaded push rod; 810. Adjusting wheel two; 811. Movable groove; 812. Spring; 813. Fixing ring; 814. Clamping block; 9. Lower plug. Detailed Implementation

[0024] To better understand the above-mentioned objectives, features, and advantages of this utility model, the present utility model will be further described below with reference to the accompanying drawings and embodiments. It should be noted that, unless otherwise specified, the embodiments and features described in these embodiments can be combined with each other.

[0025] Many specific details are set forth in the following description in order to provide a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Therefore, the present invention is not limited to the specific embodiments disclosed in the following specification.

[0026] Please see Figure 1 and Figure 4 This utility model provides a technical solution: a gravity chromatography pre-packed column for improving the A / G binding ability of proteins, comprising a column body 1;

[0027] The flow regulation mechanism 8 is located at the bottom of the column 1 and is used to regulate the flow rate of the liquid;

[0028] The flow regulation mechanism 8 includes a housing 801, a shaft hole 802, a rotating shaft 803, and a baffle 804. The shaft hole 802 is located at the output end of the housing 801. The rotating shaft 803 is rotatably connected inside the shaft hole 802. When the rotating shaft 803 rotates, it drives the baffle 804 to rotate inside the housing 801. By adjusting the angle of the baffle 804, the liquid flow rate can be regulated. When the rotating shaft 803 is driven to rotate, it can drive the baffle 804, which is fitted on the outer wall, to rotate inside the housing 801. At this time, as the baffle 804 rotates, its relative position with the housing 801 changes, thereby adjusting the flow area of ​​the liquid discharged from the column 1. When the flow area increases, the flow rate decreases; when the flow area decreases, the flow rate increases. By adjusting the flow rate, the binding time between the protein and the pre-packed column packing can be controlled to ensure that the target protein is fully bound and to reduce the low binding efficiency or incomplete elution caused by improper flow rate. This also makes the pre-packed column flexible to adapt to different experimental conditions without the need to change equipment or packing.

[0029] like Figure 4 As shown, an adjusting wheel 805 is fixedly installed on one side of the outer wall of the rotating shaft 803, and a clamping ring 806 is fixedly installed on one side of the outer wall of the outer casing 801. A connecting seat 807 is movably inserted into the inner surface of the clamping ring 806. A rotating disk 808 is fixedly installed on one side of the outer wall of the connecting seat 807, and the outer surface of the rotating disk 808 is movably inserted into the inside of the clamping ring 806. The adjusting wheel 805 allows the user to easily drive the rotating shaft 803 to rotate. The clamping ring 806 can cooperate with the rotating disk 808 to fix or loosen the rotating shaft 803, thereby preventing the rotating shaft 803 from rotating due to external force collision or vibration, which would cause changes in flow rate.

[0030] like Figure 4 and Figure 5As shown, a threaded push rod 809 is threadedly connected to the inner surface of the connecting seat 807. An adjusting wheel 810 is fixedly installed on one side of the outer wall of the threaded push rod 809. A set of movable slots 811 are opened inside the rotating disk 808. A spring 812 is movably inserted into the inner surface of each set of movable slots 811. The tail end of the threaded push rod 809 is not threaded and is movably inserted into the connecting seat 807, which facilitates the movement of related devices by the threaded push rod 809. After the adjusting wheel 810 is rotated, the threaded push rod 809 is driven to rotate and, under the action of the thread, advances inside the connecting seat 807 and extends into the rotating disk 808.

[0031] like Figure 5 and Figure 6 As shown, a set of springs 812 are each fixedly mounted with a retaining ring 813 on one side of their outer wall, and the outer walls of the retaining rings 813 are fixedly inserted into the interior of the movable groove 811. A set of movable grooves 811 are each movably inserted with a locking block 814 on the inner wall, and the outer walls of the springs 812 are fixedly connected to the outer walls of the locking blocks 814. The outer walls of the locking blocks 814 are fixedly inserted into the interior of the retaining rings 813. When the threaded push rod 809 advances, its first end will contact the arc-shaped surface of the locking block 814 in the movable groove 811 and push the locking block 814 outward, pulling the spring 812 and retracting it into the movable groove 811. At this time, the other end of the locking block 814 will be in close contact with the inner wall of the clamping ring 806, clamping the rotating shaft 803 on one side of the rotating disk 808 to prevent it from rotating accidentally, thus ensuring that the binding time between the internal protein and the pre-filled column packing remains unchanged.

[0032] like Figure 2 As shown, the outer wall of the column 1 is movably fitted with a top cover 2, and the outer wall of the column 1 is fixedly fitted with a fixing sleeve 3. The outer wall of the fixing sleeve 3 is movably fitted with a connecting rope 4. The top cover 2 can prevent liquid splashing, protect the safety of operators, and avoid contamination of protein A / G packing material or samples.

[0033] like Figure 1 and Figure 2 As shown, a connector 5 is movably inserted into the inner wall of the connecting rope 4, and the outer wall of the connector 5 is movably inserted into the inside of the column 1. The connecting rope 4 connects the top cover 2 to the column 1, which can effectively prevent the top cover 2 from being lost.

[0034] like Figure 3 As shown, a piston 6 is fixedly installed at the bottom of the upper cover 2, and the outer wall of the piston 6 is movably inserted into the inside of the column 1. The outer wall of the column 1 is provided with scale lines 7, and the outer wall of the flow regulating mechanism 8 is movably fitted with a lower plug 9. The piston 6 can improve the sealing of the liquid inside the upper cover 2, the scale lines 7 allow people to know the volume of the liquid inside in a timely manner, and the lower plug 9 prevents the buffer solution or elution solution from leaking from the bottom when the pre-installed column is not in use.

[0035] The above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model in any other way. Any person skilled in the art may make changes or modifications to the above-disclosed technical content to create equivalent embodiments for application in other fields. However, any simple modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of the present utility model without departing from the technical solution of the present utility model shall still fall within the protection scope of the technical solution of the present utility model.

Claims

1. A gravity chromatography pre-packed column for improving protein A / G binding ability, characterized in that: Includes column (1); The flow regulation mechanism (8) is located at the bottom of the column (1) and is used to regulate the flow rate of the liquid; The flow regulating mechanism (8) includes a housing (801), a shaft hole (802), a rotating shaft (803), and a baffle (804). The shaft hole (802) is located at the output end of the housing (801). The rotating shaft (803) is rotatably connected inside the shaft hole (802). When the rotating shaft (803) rotates, it drives the baffle (804) to rotate inside the housing (801). By adjusting the angle of the baffle (804), the flow rate of the liquid can be regulated.

2. The gravity chromatography pre-packed column for improving protein A / G binding ability according to claim 1, characterized in that: An adjusting wheel (805) is fixedly installed on one side of the outer wall of the rotating shaft (803), and a clamping ring (806) is fixedly installed on one side of the outer wall of the outer shell (801). A connecting seat (807) is movably inserted into the inner surface of the clamping ring (806), and a rotating disk (808) is fixedly installed on one side of the outer wall of the connecting seat (807), with the outer surface of the rotating disk (808) movably inserted into the inside of the clamping ring (806).

3. The gravity chromatography pre-packed column for improving protein A / G binding ability according to claim 2, characterized in that: The inner wall of the connecting seat (807) is threaded with a threaded push rod (809), and an adjusting wheel (810) is fixedly installed on one side of the outer wall of the threaded push rod (809). The rotating disk (808) has a set of movable grooves (811) inside, and springs (812) are movably inserted into the inner wall of each set of movable grooves (811).

4. The gravity chromatography pre-packed column for improving protein A / G binding ability according to claim 3, characterized in that: A set of springs (812) are each fixedly installed with a fixing ring (813) on one side of their outer wall, and the outer wall of the set of fixing rings (813) is fixedly inserted into the interior of the movable groove (811). The inner wall of the set of movable grooves (811) is movably inserted with a locking block (814), and the outer wall of the set of springs (812) is fixedly connected to the outer wall of the locking block (814), while the outer wall of the set of locking blocks (814) is fixedly inserted into the interior of the fixing ring (813).

5. The gravity chromatography pre-packed column for improving protein A / G binding ability according to claim 1, characterized in that: The outer wall of the column (1) is movably fitted with a top cover (2), and the outer wall of the column (1) is fixedly fitted with a fixing sleeve (3), and the outer wall of the fixing sleeve (3) is movably fitted with a connecting rope (4).

6. The gravity chromatography pre-packed column for improving protein A / G binding ability according to claim 5, characterized in that: The inner wall of the connecting rope (4) is movably inserted with a connector (5), and the outer wall of the connector (5) is movably inserted into the interior of the column (1).

7. The gravity chromatography pre-packed column for improving protein A / G binding ability according to claim 5, characterized in that: A piston (6) is fixedly installed at the bottom of the upper cover (2), and the outer wall of the piston (6) is movably inserted into the inside of the column (1). The outer wall of the column (1) is provided with scale lines (7), and the outer wall of the flow regulating mechanism (8) is movably fitted with a lower plug (9).

Images