A high-pressure homogenizer for microbial cell wall disruption

Abstract

The utility model discloses a high pressure homogenizer for microorganism wall breaking relates to high pressure homogenizer field, including high pressure homogenizer, one side of high pressure homogenizer is installed the filter box, the inside of filter box is opened the filter storehouse, the inside installation of filter storehouse has two groups first filter and second filter, the bottom of second filter is connected with filter drum, the bottom of filter drum is provided with the collection of sewage groove, the bottom of filter box is connected with the connecting pipe. The utility model microorganism liquid will be in the filter box inside the general tendency of flowing down, and the liquid will flow through multiple groups first filter and second filter, and first filter and second filter carry out the filtration to microorganism liquid, and the impurity that blocks outside first filter and second filter can slide along the inner wall of filter storehouse and fall into the collection of sewage groove, thereby make microorganism liquid in the filtration process, and the impurity contained therein will not accumulate on the outer wall of first filter and second filter.

Description

Technical Field

[0001] This utility model relates to the field of high-pressure homogenizers, specifically a high-pressure homogenizer for microbial cell wall disruption. Background Technology

[0002] A high-pressure homogenizer is a device that uses high pressure generated by a high-pressure pump to subject materials to strong shearing, impact, and cavitation effects as they pass through narrow homogenizing valve gaps, thereby achieving material refinement and homogenization. In the field of microbial cell wall disruption, the high-pressure homogenizer can effectively destroy the cell walls of microorganisms, releasing active components such as proteins, enzymes, and nucleic acids, thus facilitating subsequent extraction and purification.

[0003] During microbial fermentation, the microbial liquid often contains various impurities, such as incompletely dissolved culture medium components, byproducts of microbial metabolism, and fragments of the microorganisms themselves. The presence of these impurities can affect the cell wall disruption effect of the high-pressure homogenizer on microorganisms. If no filtration is performed, the impurities may enter the homogenizer along with the microbial cells. Under high pressure, the impurities may hinder the homogenizer from effectively destroying the cell walls of microorganisms, reducing the cell wall disruption efficiency, and may also lead to a decrease in the purity of the extracted target product.

[0004] Currently, in order to filter microbial liquids, a filter screen is usually installed at the port of the feed pipe of a high-pressure homogenizer. However, in actual use, when the high-pressure homogenizer intermittently pumps the microbial liquid, due to the high-pressure characteristics of the suction force generated by the homogenizer, impurities in the microbial liquid will be adsorbed onto the filter screen pores. As the usage time increases, impurities accumulate continuously, which can easily cause local blockage of the filter screen pores, resulting in a significant reduction in the rate at which the microbial liquid passes through the filter screen, thus affecting the feeding efficiency of the high-pressure homogenizer. Utility Model Content

[0005] Based on this, the purpose of this utility model is to provide a high-pressure homogenizer for microbial cell wall disruption, so as to solve the technical problem that impurities in the liquid are easily accumulated on the surface of the filter screen when filtering microbial liquid.

[0006] To achieve the above objectives, this utility model provides the following technical solution: a high-pressure homogenizer for microbial cell wall disruption, comprising a high-pressure homogenizer, a cell wall disruption chamber installed on one side of the high-pressure homogenizer, a liquid suction pipe provided on the top of the cell wall disruption chamber, a filter box connected to the end of the liquid suction pipe, a filter chamber provided inside the filter box, two sets of first filters and second filters installed inside the filter chamber, a filter cylinder connected to the bottom of the second filter, a sludge collection tank provided at the bottom of the filter cylinder, and a connecting pipe connected to the bottom of the filter box.

[0007] By adopting the above technical solution, the problem that impurities in the liquid easily accumulate on the surface of the filter screen when filtering microbial liquid is solved. The microbial liquid generally flows downward inside the filter box, and the liquid flows through multiple sets of first and second filters. The first and second filters filter the microbial liquid, and the impurities blocked outside the first and second filters will slide down the inner wall of the filter chamber into the collection tank. Thus, the impurities contained in the microbial liquid will not accumulate on the outer wall of the first and second filters during the filtration process.

[0008] The present invention is further configured such that the outer wall of the first filter has multiple sets of first filter holes, and the first filter holes are arranged vertically upward.

[0009] Preferably, the microbial liquid enters the interior of the first filter through the first filter hole, which is vertically upward, so that the downward-flowing microbial liquid directly enters the first filter through the first filter hole, while impurities flow downward along the inclined wall of the first filter.

[0010] The present invention is further configured such that the outer wall of the second filter has multiple sets of second filter holes, and the second filter holes are inclined downward.

[0011] Preferably, the microbial liquid can enter the second filter through the second filter hole, and the second filter absorbs the downward flowing microbial liquid while impurities flow downward.

[0012] The present invention is further configured such that a connecting ring is connected to the bottom of the first filter, and one end of the connecting ring is connected to the second filter.

[0013] Preferably, the connecting ring connects and fixes the first filter and the second filter.

[0014] The present invention is further configured such that the sludge collection tank is inclined downward, and the bottom of the sludge collection tank is connected to a drain hole, and a drain pipe is installed at the end of the drain hole.

[0015] Preferably, impurities flow along the outer walls of the first and second filters and converge at the collection tank. Since the bottom wall of the collection tank is inclined to one side, the impurities collected in the collection tank flow into the drain hole. When the valve on the drain pipe is opened, the impurities collected in the drain hole are discharged through the drain pipe.

[0016] The present invention is further configured such that a connecting pipe is installed inside the filter box, and the bottom end of the connecting pipe is connected to a connecting pipe, and the outer wall of the connecting pipe is provided with multiple sets of through holes.

[0017] Preferably, the high-pressure homogenizer intermittently draws liquid from the filter box through the suction pipe, and the connecting pipe draws negative pressure into the first and second filters through the through hole, so that the liquid inside the first and second filters flows into the connecting pipe through the connecting pipe.

[0018] The present invention is further configured such that the through holes are evenly spaced, and the diameter of the through holes is larger than the diameter of the first filter hole.

[0019] Preferably, the multiple sets of through holes inside the connecting pipe result in a smaller suction difference between the first filter, the second filter, and the filter cartridge.

[0020] The present invention is further configured such that a guide block is fixed at the top of the connecting pipe, and the guide block is configured as a semi-circle.

[0021] Preferably, when the microbial liquid enters the top of the filter chamber, the guide block directs the microbial liquid to the surrounding areas, allowing the microbial liquid to flow evenly downwards along the filter chamber.

[0022] The present invention is further configured such that a second guide plate is fixed at the bottom end of the outer wall of the second filter, and the top wall surface of the second guide plate is inclined.

[0023] Preferably, the flow rate of the microbial liquid flowing through the second guide plate is increased, so that the liquid located below the second guide plate flows quickly to the top of the first filter located below the second guide plate.

[0024] The present invention is further configured such that a first guide plate is installed on the inner wall of the filter chamber, and the first guide plate is located at the top of the second filter.

[0025] Preferably, the liquid flow rate above the first guide plate is less than the liquid flow rate outside the first filter, thereby increasing the total amount of liquid filtered by the first filter.

[0026] In summary, the present invention has the following main advantages:

[0027] 1. This utility model solves the problem that impurities in microbial liquids easily accumulate on the filter screen surface when filtering microbial liquids by setting up a high-pressure homogenizer, a filter box, a first filter, a second filter, a connecting pipe, and a sludge collection tank. The microbial liquid generally flows downward inside the filter box and flows through multiple sets of first and second filters. The first and second filters filter the microbial liquid, and impurities blocked outside the first and second filters slide down the inner wall of the filter chamber into the sludge collection tank. Thus, impurities contained in the microbial liquid do not accumulate on the outer walls of the first and second filters during the filtration process.

[0028] 2. This utility model, by setting a first guide plate and a second guide plate, with the first and second guide plates installed on the outside of the second guide device, and the first and second guide plates being staggered and located on the inner wall of the filter chamber and the outer wall of the second filter respectively, increases the flow rate of the microbial liquid flowing through the first and second guide plates. This allows the liquid below the second guide plate to flow quickly to the top of the first filter located below the second guide plate, while the flow rate of the liquid above the first guide plate is less than the flow rate of the liquid outside the first filter, thereby increasing the total amount of liquid filtered by the first filter. Attached Figure Description

[0029] Figure 1 This is a schematic diagram of the overall device of this utility model;

[0030] Figure 2 This is a structural diagram of the internal structure of the filter box of this utility model;

[0031] Figure 3 This is a structural diagram of the connecting pipe of this utility model;

[0032] Figure 4 For the present utility model Figure 2 Enlarged view of Figure A;

[0033] Figure 5 This is a schematic diagram of the second filter structure of this utility model.

[0034] Explanation of reference numerals in the attached figures:

[0035] 1. High-pressure homogenizer; 101. Breaking chamber; 102. Suction pipe; 2. Filter box; 201. Filter compartment; 202. Feed pipe; 203. Drain pipe; 204. Connecting pipe; 205. Drain hole; 206. Sludge collection tank; 3. Connecting pipe; 301. Through hole; 302. Guide block; 4. First filter; 401. First filter hole; 402. Connecting ring; 5. Second filter; 501. Second filter hole; 502. Third filter hole; 6. Filter cylinder; 7. First guide plate; 701. Second guide plate. Detailed Implementation

[0036] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0037] The embodiments of this utility model will be described below based on its overall structure.

[0038] First embodiment:

[0039] Please see Figure 1 — Figure 4 The system includes a high-pressure homogenizer 1, a wall-breaking chamber 101 installed on one side of the high-pressure homogenizer 1, a suction pipe 102 installed on the top of the wall-breaking chamber 101, and a filter box 2 connected to the end of the suction pipe 102. The filter box 2 has a filter chamber 201 inside, and two sets of first filters 4 and second filters 5 are installed inside the filter chamber 201. The bottom of the second filter 5 is connected to a filter cylinder 6, and the bottom of the filter cylinder 6 is provided with a sludge collection tank 206. The bottom of the filter box 2 is connected to a connecting pipe 204. This system solves the problem that impurities in the liquid are easily accumulated on the surface of the filter screen when filtering microbial liquid. The microbial liquid tends to flow downwards inside the filter box 2. The liquid flows through multiple sets of first filters 4 and second filters 5. The first filters 4 and second filters 5 filter the microbial liquid. Impurities blocked outside the first filters 4 and second filters 5 will slide down the inner wall of the filter chamber 201 into the sludge collection tank 206. Thus, during the filtration process, the impurities contained in the microbial liquid will not accumulate on the outer walls of the first filters 4 and second filters 5.

[0040] For details regarding the above embodiments, please refer to [link / reference]. Figure 2 The outer wall of the first filter 4 has multiple sets of first filter holes 401, and the first filter holes 401 are set vertically upward. Microbial liquid enters the interior of the first filter 4 through the first filter holes 401. The first filter holes 401 are set vertically upward, so that the downward flowing microbial liquid directly enters the first filter 4 through the first filter holes 401, while impurities flow downward along the inclined wall of the first filter 4.

[0041] For details regarding the above embodiments, please refer to [link / reference]. Figure 2 The outer wall of the second filter 5 has multiple sets of second filter holes 501, and the second filter holes 501 are set at an angle downward. Microbial liquid can enter the interior of the second filter 5 through the second filter holes 501, and the second filter 5 absorbs the downward flowing microbial liquid, while impurities flow downward.

[0042] For details regarding the above embodiments, please refer to [link / reference]. Figure 2 The bottom of the first filter 4 is connected to a connecting ring 402, and one end of the connecting ring 402 is connected to the second filter 5. The connecting ring 402 connects and fixes the first filter 4 and the second filter 5.

[0043] For details regarding the above embodiments, please refer to [link / reference]. Figure 3The sludge collection tank 206 is inclined downwards, and the bottom of the sludge collection tank 206 is connected to a drain hole 205. A drain pipe 203 is installed at the end of the drain hole 205. Impurities flow along the outer wall of the first filter 4 and the second filter 5 and converge at the sludge collection tank 206. Since the bottom wall of the sludge collection tank 206 is inclined to one side, the impurities collected in the sludge collection tank 206 flow into the drain hole 205. When the valve on the drain pipe 203 is opened, the impurities collected in the drain hole 205 are discharged through the drain pipe 203.

[0044] For details regarding the above embodiments, please refer to [link / reference]. Figure 3 The filter box 2 is equipped with a connecting pipe 3, and the bottom end of the connecting pipe 3 is connected to the connecting pipe 204. The outer wall of the connecting pipe 3 is provided with multiple sets of through holes 301. The high pressure homogenizer 1 intermittently draws the liquid in the filter box 2 through the suction pipe 102, so that the connecting pipe 3 draws negative pressure into the first filter 4 and the second filter 5 through the through holes 301. The liquid inside the first filter 4 and the second filter 5 flows into the connecting pipe 204 through the connecting pipe 3.

[0045] For details regarding the above embodiments, please refer to [link / reference]. Figure 3 The through holes 301 are evenly spaced, and the diameter of the through holes 301 is larger than the diameter of the first filter hole 401. The multiple sets of through holes 301 inside the connecting pipe 3 make the suction difference between the first filter 4, the second filter 5 and the filter cylinder 6 smaller.

[0046] For details regarding the above embodiments, please refer to [link / reference]. Figure 2 A guide block 302 is fixed at the top of the connecting pipe 3, and the guide block 302 is set as a semi-circle. When the microbial liquid enters the top of the filter chamber 201, the guide block 302 guides the microbial liquid to the surrounding area, so that the microbial liquid can flow downward evenly along the filter chamber 201.

[0047] For details regarding the above embodiments, please refer to [link / reference]. Figure 4 The bottom of the outer wall of the second filter 5 is fixed with a second guide plate 701, and the top wall of the second guide plate 701 is inclined. The flow rate of the microbial liquid flowing through the second guide plate 701 is increased, so that the liquid below the second guide plate 701 flows quickly to the top of the first filter 4 below the second guide plate 701.

[0048] For details regarding the above embodiments, please refer to [link / reference]. Figure 4 The inner wall of the filter chamber 201 is equipped with a first guide plate 7, and the first guide plate 7 is located at the top of the second filter 5. The liquid flow rate above the first guide plate 7 is less than the liquid flow rate outside the first filter 4, thereby increasing the total amount of liquid filtered by the first filter 4.

[0049] Second embodiment:

[0050] Please see Figure 5 The outer wall of the second filter 5 has multiple sets of third filter holes 502, and the third filter holes 502 are staggered with the second filter holes 501, so that the microbial liquid located outside the second filter 5 can quickly enter the second filter 5, thereby accelerating the filtration rate of the microbial liquid by the second filter 5.

[0051] In practical operation, the microbial liquid is poured into the filter chamber 2 through the feed pipe 202. When the microbial liquid enters the top of the filter chamber 201, the guide block 302 guides the microbial liquid to the surrounding areas. The microbial liquid flows downward along the filter chamber 201. During the downward flow of the microbial liquid, it flows into the first filter 4 and the second filter 5 through the first filter hole 401 and the second filter hole 501, respectively. The high-pressure homogenizer 1 intermittently draws the liquid in the filter chamber 2 through the suction pipe 102, so that the connecting pipe 3 creates a negative pressure inside the first filter 4 and the second filter 5 through the through hole 301. The negative pressure inside the first filter 4 and the second filter 5 can draw the microbial liquid inside the filter chamber 201, thereby increasing the efficiency of the process. The microbial liquid enters the first filter 4 and the second filter 5 through the first filter hole 401 and the second filter hole 501. The liquid in the first filter 4 and the second filter 5 enters the connecting pipe 3 through the through hole 301, and finally flows into the suction pipe 102 through the connecting pipe 204. The impurities in the microbial liquid are blocked in the filter chamber 201 by the first filter 4 and the second filter 5. With the downward flow of the microbial liquid, the impurities flow along the outer wall of the first filter 4 and the second filter 5 and converge at the collection tank 206. Since the bottom wall of the collection tank 206 is inclined to one side, the impurities collected in the collection tank 206 flow into the drain hole 205. When the valve on the drain pipe 203 is opened, the impurities collected in the drain hole 205 are discharged through the drain pipe 203.

[0052] Although embodiments of the present invention have been shown and described, these specific embodiments are merely explanations of the present invention and are not intended to limit the invention. The specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. After reading this specification, those skilled in the art may make modifications, substitutions, and variations to the embodiments as needed without departing from the principles and spirit of the present invention, provided that such modifications, substitutions, and variations are within the scope of the claims of the present invention and are protected by patent law.

Claims

1. A high-pressure homogenizer for microbial cell disruption, comprising a high-pressure homogenizer (1), characterized in that: A wall-breaking box (101) is installed on one side of the high-pressure homogenizer (1). A suction pipe (102) is provided on the top of the wall-breaking box (101). A filter box (2) is connected to the end of the suction pipe (102). A filter chamber (201) is opened inside the filter box (201). Two sets of first filters (4) and second filters (5) are installed inside the filter chamber (201). A filter cylinder (6) is connected to the bottom of the second filter (5). A sludge collection tank (206) is provided at the bottom of the filter cylinder (6). A connecting pipe (204) is connected to the bottom of the filter box (2).

2. The high-pressure homogenizer for microbial cell wall disruption according to claim 1, characterized in that: The outer wall of the first filter (4) has multiple sets of first filter holes (401), and the first filter holes (401) are arranged vertically upward.

3. The high-pressure homogenizer for microbial cell wall disruption according to claim 1, characterized in that: The outer wall of the second filter (5) has multiple sets of second filter holes (501), and the second filter holes (501) are arranged at an angle downward.

4. A high-pressure homogenizer for microbial cell wall disruption according to claim 1, characterized in that: The bottom of the first filter (4) is connected to a connecting ring (402), and one end of the connecting ring (402) is connected to the second filter (5).

5. A high-pressure homogenizer for microbial cell wall disruption according to claim 1, characterized in that: The sludge collection tank (206) is inclined downward, and a drain hole (205) is connected to the bottom of the sludge collection tank (206). A drain pipe (203) is installed at the end of the drain hole (205).

6. The high-pressure homogenizer for microbial cell wall disruption according to claim 1, characterized in that: The filter box (2) is equipped with a connecting pipe (3) inside, and the bottom end of the connecting pipe (3) is connected to a connecting pipe (204). The outer wall of the connecting pipe (3) is provided with multiple sets of through holes (301).

7. A high-pressure homogenizer for microbial cell wall disruption according to claim 6, characterized in that: The through holes (301) are evenly spaced, and the diameter of the through holes (301) is larger than the diameter of the first filter hole (401).

8. A high-pressure homogenizer for microbial cell wall disruption according to claim 6, characterized in that: The top of the connecting pipe (3) is fixed with a guide block (302), and the guide block (302) is set as a semi-circle.

9. A high-pressure homogenizer for microbial cell wall disruption according to claim 1, characterized in that: The bottom of the outer wall of the second filter (5) is fixed with a second guide plate (701), and the top wall of the second guide plate (701) is inclined.

10. A high-pressure homogenizer for microbial cell wall disruption according to claim 1, characterized in that: The inner wall of the filter chamber (201) is equipped with a first guide plate (7), and the first guide plate (7) is located at the top of the second filter (5).

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